##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780706_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8021 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60740556040394 34.0 34.0 34.0 31.0 34.0 2 33.08876698666002 34.0 34.0 34.0 32.0 34.0 3 33.38436603914724 34.0 34.0 34.0 33.0 34.0 4 33.450317915471885 34.0 34.0 34.0 34.0 34.0 5 33.463283879815485 34.0 34.0 34.0 34.0 34.0 6 37.33574367285875 38.0 37.0 38.0 36.0 38.0 7 36.82433611769106 38.0 37.0 38.0 35.0 38.0 8 36.92020945019324 38.0 37.0 38.0 35.0 38.0 9 36.58384241366413 38.0 37.0 38.0 34.0 38.0 10-11 36.313115571624486 38.0 37.0 38.0 33.0 38.0 12-13 36.000623363670364 38.0 36.5 38.0 32.5 38.0 14-15 36.11332751527241 38.0 36.5 38.0 32.5 38.0 16-17 35.95368407929186 38.0 36.0 38.0 32.0 38.0 18-19 35.78537588829323 38.0 36.0 38.0 31.0 38.0 20-21 35.67522752773968 38.0 36.0 38.0 31.5 38.0 22-23 35.92619374142875 38.0 36.5 38.0 32.5 38.0 24-25 34.57854382246603 37.0 34.0 38.0 28.0 38.0 26-27 34.919648422889914 37.0 34.0 38.0 29.0 38.0 28-29 34.76785936915597 37.0 34.0 38.0 29.0 38.0 30-31 34.0923201595811 37.0 34.0 38.0 25.5 38.0 32-33 34.74760004986909 37.0 35.0 38.0 27.0 38.0 34-35 33.94102979678344 37.0 34.0 38.0 24.0 38.0 36-37 34.01340231891285 37.0 34.0 38.0 24.0 38.0 38-39 28.709761875077923 34.0 23.0 37.0 11.0 38.0 40-41 32.00211943647924 35.0 30.5 37.0 17.0 38.0 42-43 34.606595187632465 37.0 34.5 38.0 28.5 38.0 44-45 35.172048373020814 37.0 35.5 38.0 31.0 38.0 46-47 35.24292482234135 37.5 36.0 38.0 28.5 38.0 48-49 34.93722727839422 37.5 35.0 38.0 28.0 38.0 50-51 34.6001745418277 37.0 34.0 38.0 28.0 38.0 52-53 34.17017828200973 37.0 34.0 38.0 24.5 38.0 54-55 34.73232763994514 37.0 34.5 38.0 27.0 38.0 56-57 34.7813863608029 37.0 34.5 38.0 27.0 38.0 58-59 34.07106345842165 37.0 34.0 38.0 24.0 38.0 60-61 33.97550180775464 37.0 34.0 38.0 24.5 38.0 62-63 34.75969330507418 37.0 34.5 38.0 27.0 38.0 64-65 34.91179404064331 37.0 35.5 38.0 28.0 38.0 66-67 32.93255205086648 37.0 31.0 38.0 21.5 38.0 68-69 29.854506919336742 34.0 24.0 37.5 11.0 38.0 70-71 26.27029048747039 28.5 17.0 35.5 10.5 37.5 72-73 20.973382371275402 21.5 11.0 28.0 10.5 35.5 74-75 20.3042638075053 21.0 11.0 26.0 11.0 34.0 76 20.596309687071436 21.0 11.0 27.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8602904020112732 1101 2 0.9860970340609185 1101 3 0.8045372288572779 1101 4 -0.5555604036792019 1101 5 0.4973050313818206 1101 6 -0.838398820817666 1101 7 0.46302521621534964 1101 8 -0.11919254070411256 1101 9 1.0110506131278498 1101 10-11 0.7526771222093132 1101 12-13 1.7067396737818683 1101 14-15 1.7867610353558518 1101 16-17 -0.7588636555135295 1101 18-19 0.23208906728586243 1101 20-21 -0.16584956109383597 1101 22-23 0.6875038641798241 1101 24-25 -2.905238269776511 1101 26-27 0.02048891197099323 1101 28-29 2.7295373666790113 1101 30-31 2.980308523887409 1101 32-33 2.79933316650429 1101 34-35 2.400575817078831 1101 36-37 1.8725068366615645 1101 38-39 1.348234533152059 1101 40-41 3.6868249662158696 1101 42-43 1.6160109281673485 1101 44-45 1.4599700232133443 1101 46-47 1.6902977242479977 1101 48-49 -3.7486989186882838 1101 50-51 -2.3315847843223665 1101 52-53 -2.5984454748346764 1101 54-55 -0.9787248893284257 1101 56-57 -1.242569903594557 1101 58-59 -1.2429277471285403 1101 60-61 -2.4745973876923166 1101 62-63 0.24102942782727155 1101 64-65 -0.4619690020185274 1101 66-67 -2.4628980925790245 1101 68-69 -0.39729013587276185 1101 70-71 -3.6088358760032975 1101 72-73 -2.18188409129446 1101 74-75 0.03510558496843785 1101 76 1.2389439090842878 1102 1 -2.264215526842481 1102 2 0.025622725760520382 1102 3 -0.8318264075063553 1102 4 -0.33421653016141306 1102 5 -0.05210208324267995 1102 6 -0.40954506587694794 1102 7 -0.5369747837846504 1102 8 0.45392998894015335 1102 9 0.6632245261713265 1102 10-11 0.3841000471105005 1102 12-13 0.359901729117837 1102 14-15 0.4883420630238362 1102 16-17 0.5158399017987279 1102 18-19 -0.11474887737816886 1102 20-21 1.4803954981946958 1102 22-23 0.3011402278161839 1102 24-25 -5.860771866614456 1102 26-27 -2.008167214511218 1102 28-29 -0.652873700514661 1102 30-31 2.182877693847878 1102 32-33 0.4574359333027118 1102 34-35 0.7503781886203313 1102 36-37 -2.1610900012830996 1102 38-39 1.4351910548911917 1102 40-41 2.5000660729352298 1102 42-43 0.8818212048471921 1102 44-45 1.688230892778563 1102 46-47 0.5756732183191389 1102 48-49 -4.804034887067729 1102 50-51 -1.1290156143618972 1102 52-53 -0.8365877673247937 1102 54-55 0.8779944782605114 1102 56-57 1.7811455114252084 1102 58-59 0.4744635572192877 1102 60-61 -8.142581577415637 1102 62-63 -2.1463223508288536 1102 64-65 0.08249740114352733 1102 66-67 3.803900326393311 1102 68-69 0.34184029890984746 1102 70-71 0.6717965350638941 1102 72-73 -1.8093544470256866 1102 74-75 1.6635640829921492 1102 76 -0.6207398853821147 1103 1 0.9455746829477292 1103 2 1.0011472012850007 1103 3 0.6122295365495845 1103 4 0.30564360969872695 1103 5 -0.2618922930328935 1103 6 -0.11933527566716151 1103 7 0.6168713700615029 1103 8 0.502881037891207 1103 9 0.5093783723251732 1103 10-11 0.4767573897678403 1103 12-13 0.20255907177517685 1103 14-15 -1.2057138810321106 1103 16-17 0.27458116053998793 1103 18-19 -1.233629996259289 1103 20-21 -2.0755485577493573 1103 22-23 -0.5432653665894023 1103 24-25 -4.57580683164942 1103 26-27 -1.618307074651085 1103 28-29 -1.242034539675501 1103 30-31 -0.322367061396875 1103 32-33 1.3979953738621518 1103 34-35 0.2626159508580912 1103 36-37 -0.04220888240197951 1103 38-39 -0.160962791262655 1103 40-41 -2.146787073917917 1103 42-43 -0.020276697250707798 1103 44-45 0.9959232004708696 1103 46-47 0.7312676239135527 1103 48-49 -2.819769152801996 1103 50-51 -1.0381065234528037 1103 52-53 0.48683880610177255 1103 54-55 -0.5573202070541683 1103 56-57 -0.5143090340293384 1103 58-59 -0.7563056735499387 1103 60-61 -5.663560598394657 1103 62-63 -3.3718468263533303 1103 64-65 -1.940229871583746 1103 66-67 -2.544001771508789 1103 68-69 0.14953260660215406 1103 70-71 0.7941741574415175 1103 72-73 -2.415997803669047 1103 74-75 -2.271750602322534 1103 76 -2.0018587665009946 1104 1 1.123230360603408 1104 2 0.3546270547648547 1104 3 0.8045372288572779 1104 4 0.5272553313104424 1104 5 -1.0824051135457111 1104 6 0.06015190382002089 1104 7 1.2725490257391598 1104 8 1.0340165690267327 1104 9 1.3775102404570418 1104 10-11 -0.043389130378677976 1104 12-13 -1.1152064959903853 1104 14-15 1.4472165218982909 1104 16-17 0.24527713123595873 1104 18-19 0.8625238498945578 1104 20-21 -2.4546694368702333 1104 22-23 1.6160752927512547 1104 24-25 0.021262765420175356 1104 26-27 -0.4919334482774502 1104 28-29 0.856866559225594 1104 30-31 -0.4762132152430283 1104 32-33 0.3210722969390716 1104 34-35 1.4906379288800693 1104 36-37 2.968780128587035 1104 38-39 3.006619626319761 1104 40-41 3.128853951723105 1104 42-43 -1.1127675397415544 1104 44-45 0.6763261308738038 1104 46-47 2.3310844737303995 1104 48-49 -0.3170219000547405 1104 50-51 0.35475061940433505 1104 52-53 1.3311611504241228 1104 54-55 0.1496394999055397 1104 56-57 -0.12145189117219957 1104 58-59 -2.454107871352143 1104 60-61 0.7448643100302448 1104 62-63 0.7627685582620529 1104 64-65 1.964532033178159 1104 66-67 0.07229859479157597 1104 68-69 4.3894593465288985 1104 70-71 0.7886796519470103 1104 72-73 -0.9251553128265577 1104 74-75 -0.5528861334580668 1104 76 -3.943250707892936 1105 1 1.3235587678283665 1105 2 0.8751525063247882 1105 3 0.5976406771331426 1105 4 -0.14926355210499054 1105 5 0.06702017381061154 1105 6 0.24405994979702683 1105 7 1.221645905870524 1105 8 -1.1761640549469945 1105 9 0.6977072847920169 1105 10-11 0.29240725714184634 1105 12-13 0.6820020425974604 1105 14-15 1.0933577370050216 1105 16-17 -0.043721226727917895 1105 18-19 0.15562729817041543 1105 20-21 1.1512418931790265 1105 22-23 0.8771590365936177 1105 24-25 1.2199491365203343 1105 26-27 0.5991995566486565 1105 28-29 0.8494773966640281 1105 30-31 2.88585575027421 1105 32-33 2.027968848663207 1105 34-35 0.8600960569588878 1105 36-37 0.14612003006486418 1105 38-39 0.2800186410980885 1105 40-41 -1.4458586919550491 1105 42-43 -0.11112550361676199 1105 44-45 1.2313343410544206 1105 46-47 0.5913471995103663 1105 48-49 -0.6347558902025199 1105 50-51 1.0222383041334027 1105 52-53 0.7613746151203458 1105 54-55 1.3803455754392004 1105 56-57 1.7529323452810104 1105 58-59 2.508946315839978 1105 60-61 0.91070995863452 1105 62-63 -1.2717142003586375 1105 64-65 0.3183908180714283 1105 66-67 -0.7117736547979163 1105 68-69 -1.106435563159117 1105 70-71 0.09656142534602807 1105 72-73 -0.20512247210405832 1105 74-75 -2.06684344051882 1105 76 -0.7790470954134605 1106 1 -3.019626782253738 1106 2 0.3070080071458037 1106 3 0.13787056219061355 1106 4 0.5748743789294934 1106 5 -0.17764320878380602 1106 6 0.1553899990581158 1106 7 -2.3464985933084606 1106 8 0.795921330931499 1106 9 -0.051061188114388756 1106 10-11 0.7423251553356067 1106 12-13 2.1228887421048483 1106 14-15 -0.5051644304826652 1106 16-17 0.34051522647404653 1106 18-19 1.1482381356088496 1106 20-21 0.4500924678916647 1106 22-23 0.4732181498941088 1106 24-25 1.3784056225630295 1106 26-27 1.769971313627309 1106 28-29 1.1187713211303674 1106 30-31 1.0237867847569717 1106 32-33 0.20202467789145118 1106 34-35 0.8477807860229234 1106 36-37 1.778303938110838 1106 38-39 -2.3267137070135675 1106 40-41 0.5336158564850173 1106 42-43 1.006280079306066 1106 44-45 2.1048975594452344 1106 46-47 1.75965590230183 1106 48-49 2.754406671373829 1106 50-51 -0.12143985678613234 1106 52-53 0.25973257899555335 1106 54-55 -0.4694081191420807 1106 56-57 1.3309290612087565 1106 58-59 1.2601778429335724 1106 60-61 1.1734357386016754 1106 62-63 -0.21342191792842158 1106 64-65 -1.1545155858694542 1106 66-67 1.0008700233629995 1106 68-69 2.0085069655765153 1106 70-71 1.2172510805184409 1106 72-73 -0.8061076937789373 1106 74-75 -1.2433623239342566 1106 76 0.29484453020230106 1107 1 1.479420836793885 1107 2 0.8365318166696127 1107 3 0.8045372288572779 1107 4 0.454874378929496 1107 5 0.5842615531209532 1107 6 0.6068185704866877 1107 7 0.6630252162153525 1107 8 -0.08788819287802596 1107 9 -1.5767754738286754 1107 10-11 0.7213727743832266 1107 12-13 -1.9028255436094312 1107 14-15 1.0319784266601957 1107 16-17 1.4576580836169057 1107 18-19 1.7425238498945532 1107 20-21 1.1567591345583352 1107 22-23 -0.4324961358201804 1107 24-25 0.29650086065826997 1107 26-27 -1.2090763054203109 1107 28-29 1.682580844939885 1107 30-31 1.843786784756972 1107 32-33 2.7610722969390693 1107 34-35 2.284923643165783 1107 36-37 2.5316372714441755 1107 38-39 2.9351910548911917 1107 40-41 1.395520618389778 1107 42-43 1.7681848412108252 1107 44-45 1.6582308927785618 1107 46-47 2.6120368546827777 1107 48-49 1.388692385659546 1107 50-51 2.2318934765471994 1107 52-53 1.3606849599479318 1107 54-55 2.7534490237150564 1107 56-57 2.4556909659706605 1107 58-59 0.554463557219286 1107 60-61 1.5610547862207298 1107 62-63 1.5827685582620532 1107 64-65 1.6597701284162483 1107 66-67 0.007536690029674276 1107 68-69 0.6618402989098477 1107 70-71 -1.24274891948156 1107 72-73 -1.0775362652075096 1107 74-75 -2.8909813715533055 1107 76 -3.40801261265484 1108 1 -0.6698099324368876 1108 2 -0.4219297217919262 1108 3 0.18915261347266465 1108 4 0.34410514816025994 1108 5 0.507338476197873 1108 6 0.3037416474097654 1108 7 0.8091790623691963 1108 8 -0.34327280826263973 1108 9 0.08630144924824634 1108 10-11 0.6306035436139936 1108 12-13 1.56794368715979 1108 14-15 0.563516888198663 1108 16-17 -0.47541883946001207 1108 18-19 0.22790846527917097 1108 20-21 1.328297596096803 1108 22-23 -1.1201884435124825 1108 24-25 1.3088085529659637 1108 26-27 -0.42599938234339163 1108 28-29 -1.4728037704447274 1108 30-31 0.10070986168005192 1108 32-33 1.9172261430929112 1108 34-35 -0.2566148183726753 1108 36-37 1.3424065022134002 1108 38-39 1.7428833625834983 1108 40-41 3.0455206183897765 1108 42-43 -0.020276697250714903 1108 44-45 2.3613078158554828 1108 46-47 -0.057193914547994495 1108 48-49 -0.05053838357122942 1108 50-51 0.884970399624116 1108 52-53 -0.09008427082130055 1108 54-55 -1.4996278993618617 1108 56-57 -0.5912321109524186 1108 58-59 1.5513866341423679 1108 60-61 1.2402855554514929 1108 62-63 1.1473839428774397 1108 64-65 1.5213085899547139 1108 66-67 0.5136905361835176 1108 68-69 2.2456864527560008 1108 70-71 -1.9365950733277124 1108 72-73 -1.9352285728998133 1108 74-75 -0.8679044484763807 1108 76 -4.117243381885611 1109 1 0.4883097256827682 1109 2 0.9313466314844305 1109 3 0.5452779695980183 1109 4 0.5378373418924554 1109 5 0.5472245160839151 1109 6 0.2823741260422423 1109 7 0.46302521621534964 1109 8 0.7165562515664163 1109 9 1.1076689706157694 1109 10-11 0.80581721882767 1109 12-13 0.540878160094266 1109 14-15 1.0742006488824174 1109 16-17 1.5680284539872744 1109 18-19 1.3995608869315959 1109 20-21 1.2278702456694504 1109 22-23 0.631948308624267 1109 24-25 1.5609453051027131 1109 26-27 -0.8490763054203114 1109 28-29 -2.7092710069119654 1109 30-31 0.9867497477199336 1109 32-33 2.932183408050186 1109 34-35 -0.5358170975749559 1109 36-37 2.8338594936663952 1109 38-39 1.324079943780081 1109 40-41 1.2955206183897765 1109 42-43 1.4533700263960156 1109 44-45 1.6974901520378225 1109 46-47 2.212036854682779 1109 48-49 1.413136830103987 1109 50-51 0.8785601432138677 1109 52-53 -1.4333891141261432 1109 54-55 -0.17840282813678954 1109 56-57 -0.30134607106637645 1109 58-59 0.7337228164785472 1109 60-61 2.054388119554055 1109 62-63 0.05906485455835053 1109 64-65 1.6523627210088492 1109 66-67 0.2601292826222661 1109 68-69 1.564062521132069 1109 70-71 0.1802140434814028 1109 72-73 0.029871142199901612 1109 74-75 0.9682778877059555 1109 76 -0.1443089089511389 1110 1 0.1646382280982337 1110 2 -0.9269464441999524 1110 3 0.19584157668336388 1110 4 0.4444395963207981 1110 5 0.5407832922513904 1110 6 0.33551422266059916 1110 7 0.984764346650131 1110 8 0.7938509375567548 1110 9 1.3153984392148033 1110 10-11 -0.1168880951819915 1110 12-13 1.0980440216079543 1110 14-15 1.2215436440515006 1110 16-17 1.0237450401386425 1110 18-19 0.5146977629380416 1110 20-21 2.160237395427899 1110 22-23 0.2744603859189567 1110 24-25 0.11650086065827026 1110 26-27 1.3248367380579538 1110 28-29 -0.18350611158184904 1110 30-31 1.980308523887409 1110 32-33 1.0819418621564623 1110 34-35 2.552749730122308 1110 36-37 3.111637271444174 1110 38-39 3.065625837499887 1110 40-41 0.8172597488245579 1110 42-43 0.8986196238195205 1110 44-45 1.8947526319090002 1110 46-47 0.8642107677262629 1110 48-49 2.5339097769638883 1110 50-51 1.0162413026341497 1110 52-53 1.4885110469044562 1110 54-55 0.4995359802367929 1110 56-57 2.8009083572750058 1110 58-59 -0.9820581819111496 1110 60-61 0.48192435143811707 1110 62-63 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-4.8640420540313265 2116 70-71 2.33489813934197 2116 72-73 -0.20106567697220967 2116 74-75 0.7678421578584604 2116 76 0.4461050344039812 2117 1 0.30530318973505643 2117 2 -0.17758583038921216 2117 3 0.6280666406219808 2117 4 0.3984037906941964 2117 5 0.40779096488565614 2117 6 0.3738773940160982 2117 7 0.46302521621534964 2117 8 0.15446474829844448 2117 9 -1.3367754738286735 2117 10-11 -1.226862519734425 2117 12-13 -2.2510608377270813 2117 14-15 0.08727254430725395 2117 16-17 -2.6258713281478023 2117 18-19 -3.5198290912819132 2117 20-21 -0.8538291007357799 2117 22-23 0.8051509230033531 2117 24-25 -2.20702855110644 2117 26-27 -4.290252776008543 2117 28-29 -4.033889743295408 2117 30-31 -3.8879779211253833 2117 32-33 -5.678927703060928 2117 34-35 -1.5891940038930379 2117 36-37 -3.358950963849942 2117 38-39 -2.9177501215793953 2117 40-41 -2.1750676169043395 2117 42-43 0.23877307650494117 2117 44-45 -3.326474989574379 2117 46-47 -3.40561020414075 2117 48-49 -0.9724840849286878 2117 50-51 -2.9057535822763327 2117 52-53 -1.153432687110893 2117 54-55 -2.414786270402587 2117 56-57 -1.0584266810881644 2117 58-59 -3.8196540898395384 2117 60-61 -3.7201216843675056 2117 62-63 -0.09017261820854117 2117 64-65 -2.087288695113159 2117 66-67 -1.4501103687938581 2117 68-69 -0.3934538187372105 2117 70-71 1.1584275511066764 2117 72-73 -2.2598892063839777 2117 74-75 0.41490098138787346 2117 76 -3.024483200890135 2118 1 1.0369208367938825 2118 2 -0.13346818333038613 2118 3 0.7420372288572779 2118 4 0.5748743789294934 2118 5 0.5842615531209532 2118 6 0.2893185704866852 2118 7 1.0255252162153496 2118 8 0.8971118071219735 2118 9 -2.3992754738286735 2118 10-11 -1.1386272256167729 2118 12-13 -0.5378255436094364 2118 14-15 0.945728426660196 2118 16-17 -2.3023419163830923 2118 18-19 -1.2312261501054422 2118 20-21 -1.851990865441664 2118 22-23 -0.3437461358201759 2118 24-25 0.24150086065827026 2118 26-27 -1.5365763054203114 2118 28-29 0.11133084493988576 2118 30-31 0.4925367847569717 2118 32-33 2.3210722969390716 2118 34-35 0.6424236431657846 2118 36-37 -2.700862728555826 2118 38-39 -3.9398089451088083 2118 40-41 -0.9544793816102235 2118 42-43 0.1431848412108252 2118 44-45 -4.280519107221437 2118 46-47 -0.06921314531722089 2118 48-49 1.2499423856595442 2118 50-51 -1.8506065234528037 2118 52-53 -2.484315040052067 2118 54-55 -2.389050976284942 2118 56-57 -0.20180903402933836 2118 58-59 -2.4317864427807123 2118 60-61 -1.1851952137792736 2118 62-63 -1.424731441737947 2118 64-65 0.30977012841625395 2118 66-67 -0.5512133099703291 2118 68-69 0.9043402989098475 2118 70-71 1.3735010805184409 2118 72-73 -0.19003626520750672 2118 74-75 -5.415981371553304 2118 76 -1.5980126126548413 2119 1 1.3851351225081672 2119 2 -1.0977538976161014 2119 3 0.44739437171442376 2119 4 -1.567982763927649 2119 5 0.4414044102638073 2119 6 -0.5588957152275995 2119 7 -2.822689069498935 2119 8 0.4149689499791194 2119 9 0.8775102404570418 2119 10-11 -0.10291293990248818 2119 12-13 -0.591396972180867 2119 14-15 -0.46945014476837343 2119 16-17 -1.088056202097377 2119 18-19 0.6125238498945578 2119 20-21 -1.9189551511559486 2119 22-23 -0.5267818501058912 2119 24-25 0.6165008606582703 2119 26-27 0.972352266008258 2119 28-29 1.2140094163684552 2119 30-31 2.0237867847569717 2119 32-33 1.392500868367641 2119 34-35 1.8834950717372152 2119 36-37 -0.8526484428415344 2119 38-39 -1.6362375165373777 2119 40-41 -2.668765095895939 2119 42-43 0.1253276983536864 2119 44-45 1.7596594642071324 2119 46-47 -0.4308202881743597 2119 48-49 -1.4955933286261711 2119 50-51 1.8904649051186269 2119 52-53 0.640684959947933 2119 54-55 1.7448775951436275 2119 56-57 -4.157166176886484 2119 58-59 -0.9183935856378511 2119 60-61 0.9710547862207264 2119 62-63 -0.022945727452238884 2119 64-65 0.7383415569876846 2119 66-67 1.881822404315379 2119 68-69 0.6275545846241322 2119 70-71 -1.0327489194815591 2119 72-73 -2.948964836636076 2119 74-75 3.5661614855895536 2119 76 1.0091302444880164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 0.0 23 4.0 24 14.0 25 32.0 26 77.0 27 146.0 28 207.0 29 323.0 30 494.0 31 643.0 32 997.0 33 1277.0 34 1650.0 35 1553.0 36 432.0 37 171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 2.5308565016830817 15.322279017578854 11.781573369904002 70.36529111083406 2 1.4337364418401695 3.216556539084902 83.96708639820471 11.382620620870215 3 1.408801895025558 15.78356813364917 74.19274404687695 8.614885924448323 4 1.2342600673232764 62.04961974816108 20.95748659768109 15.75863358683456 5 0.9599800523625484 27.440468769480113 68.44533100610897 3.154220172048373 6 1.8077546440593444 69.41777833187882 10.273033287619999 18.50143373644184 7 2.368781947388106 27.71474878444084 8.440344096746042 61.47612517142501 8 2.1568382994639075 69.11856377010348 14.636578980177035 14.088018950255579 9 2.082034659020072 14.374766238623613 2.9796783443460915 80.56352075801023 10-11 2.3438474005734946 38.87295848397955 40.0448821842663 18.73831193118065 12-13 8.334372272783943 6.052861239246977 40.94252586959232 44.67024061837676 14-15 10.198229647176163 9.475127789552424 9.705772347587583 70.62087021568382 16-17 37.66986660017454 33.58683455928188 2.3313801271661885 26.411918713377386 18-19 38.77945393342476 16.48796908116195 1.9324273781324026 42.800149607280886 20-21 11.625732452312679 5.604039396583968 2.3002119436479243 80.47001620745543 22-23 10.528612392469768 42.56327141254207 2.3438474005734946 44.564268794414666 24-25 37.42675476873208 42.94975688816856 2.7926692432365043 16.830819099862858 26-27 38.71088392968458 47.562648048871715 8.527615010597183 5.198853010846528 28-29 5.329759381623239 46.858247101358934 5.261189377883057 42.550804139134776 30-31 2.1630719361675603 12.866226156339607 41.89003864854756 43.080663258945265 32-33 8.30943772596933 36.16132651789054 8.15983044508166 47.36940531105847 34-35 4.594190250592196 13.333748909113577 40.91759132277771 41.15446951751652 36-37 35.307318289490084 33.505797282134395 14.32489714499439 16.861987283381126 38-39 11.80027428001496 13.819972571998504 34.764991896272285 39.61476125171425 40-41 4.743797531479865 9.905248722104476 45.20009973818726 40.1508540082284 42-43 41.10460042388729 4.4134147861862605 43.94713876075302 10.53484602917342 44-45 7.5988031417528985 34.9083655404563 41.59082408677222 15.902007231018574 46-47 41.71549682084528 5.7100112205460665 16.849520009973816 35.72497194863483 48-49 36.09275651415035 2.6991646926817108 11.120807879316793 50.08727091385114 50-51 9.169679591073432 2.736566512903628 45.19386610148361 42.89988779453933 52-53 7.36815858371774 3.303827452936043 76.5428250841541 12.785188879192122 54-55 3.721481112080788 2.8737065203839918 50.698167310809126 42.706645056726096 56-57 40.923824959481365 3.1666874454556786 18.987657399326768 36.92183019573619 58-59 7.174915845904501 16.138885425757387 65.98927814486971 10.696920583468396 60-61 34.52811370153348 48.57249719486349 13.290113452188008 3.609275651415036 62-63 4.6378257075177665 89.33424760004986 2.1630719361675603 3.8648547562648052 64-65 1.3776337115072934 92.78144869716994 1.8326891908739558 4.008228400448822 66-67 1.7267173669118563 90.64331130781697 2.0571001122054606 5.572871213065703 68-69 1.7516519137264681 85.08914100486223 2.992145617753397 10.167061463657898 70-71 2.5495574117940407 77.02281511033537 5.385862111956116 15.041765365914475 72-73 4.076798404189004 61.05223787557661 9.045006857000375 25.825956863234012 74-75 4.868470265552924 58.284503179154726 9.817977808253335 27.029048747039024 76 3.378631093379878 60.40393965839671 7.96658770726842 28.25084154095499 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 2.5 23 3.5 24 2.5 25 1.0 26 2.0 27 2.5 28 3.5 29 5.0 30 6.0 31 5.0 32 6.5 33 10.0 34 13.5 35 17.5 36 19.0 37 20.0 38 39.5 39 137.0 40 648.0 41 1538.0 42 1995.0 43 1923.0 44 1528.0 45 926.5 46 648.0 47 486.0 48 251.0 49 148.5 50 119.0 51 84.0 52 38.0 53 30.5 54 34.0 55 30.5 56 23.0 57 25.0 58 31.0 59 25.0 60 14.0 61 11.0 62 13.0 63 12.0 64 10.5 65 5.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 8021.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.23700286747288 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79844458846404 14.7737189876574 2 10.434219053791317 4.014462037152475 3 5.055087491898899 2.9173419773095626 4 1.7498379779650033 1.346465527989029 5 0.9721322099805573 0.9350455055479366 6 0.712896953985742 0.8228400448821843 7 0.38885288399222295 0.5236254831068445 8 0.38885288399222295 0.5984291235506795 9 0.32404406999351915 0.5610273033287619 >10 2.5275437459494494 9.736940531105848 >50 0.12961762799740764 1.333998254581723 >100 0.38885288399222295 15.584091759132276 >500 0.06480881399870382 8.353073182894901 >1k 0.06480881399870382 38.49894028176038 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 3088 38.49894028176038 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 670 8.353073182894901 RNA PCR Primer, Index 42 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 398 4.961974816107717 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 280 3.49083655404563 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT 236 2.9422765241241744 No Hit CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 114 1.4212691684328638 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT 111 1.3838673482109463 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT 111 1.3838673482109463 No Hit TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT 54 0.6732327639945145 RNA PCR Primer, Index 42 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 53 0.6607654905872086 RNA PCR Primer, Index 32 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG 37 0.4612891160703154 No Hit CTTATACACATCTCCGAGCCCACGCGACATCACTCGATCTCGTATGCCGT 34 0.423887295848398 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 32 0.3989527490337863 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC 31 0.38648547562648045 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGTCTT 31 0.38648547562648045 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGACATCGTATGCCGT 27 0.33661638199725724 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT 27 0.33661638199725724 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG 27 0.33661638199725724 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC 26 0.3241491085899514 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTT 26 0.3241491085899514 No Hit CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT 25 0.31168183518264553 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT 25 0.31168183518264553 No Hit CTTATACACATCTCCGAGCACACGAGACATCACTCGATCTCGTATGCCGT 25 0.31168183518264553 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC 23 0.2867472883680339 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT 23 0.2867472883680339 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTT 20 0.24934546814611644 No Hit CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT 19 0.23687819473881064 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG 19 0.23687819473881064 No Hit CTTATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGT 18 0.2244109213315048 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT 18 0.2244109213315048 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTAGTATGCCGT 18 0.2244109213315048 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCCCGTATGCCGT 17 0.211943647924199 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCGT 17 0.211943647924199 No Hit ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT 17 0.211943647924199 RNA PCR Primer, Index 42 (95% over 24bp) ATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTGCTT 17 0.211943647924199 RNA PCR Primer, Index 32 (96% over 29bp) CTTATACACATATCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 16 0.19947637451689315 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT 15 0.18700910110958732 No Hit TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 15 0.18700910110958732 RNA PCR Primer, Index 32 (95% over 21bp) CTTATCACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 15 0.18700910110958732 RNA PCR Primer, Index 32 (95% over 21bp) CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 14 0.17454182770228152 No Hit CTTATAAACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 13 0.1620745542949757 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCC 13 0.1620745542949757 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT 13 0.1620745542949757 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCACGTATGCCGT 12 0.14960728088766986 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATTTCGTATGCCGT 12 0.14960728088766986 No Hit ATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGTCTT 12 0.14960728088766986 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTACGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 11 0.13714000748036406 No Hit CTTATACACATCTCCGAGCCCAAGAGACATCACTCGATCTCGTATGCCGT 11 0.13714000748036406 No Hit ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 10 0.12467273407305822 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGTCTT 9 0.1122054606657524 No Hit CTTATACACATCTCCGAGCCCACGAGAAATCACTCGATCTCGTATGCCGT 9 0.1122054606657524 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT 9 0.1122054606657524 No Hit CTTATACACATCTCCGAGCCCACGAGACAACACTCGATCTCGTATGCCGT 9 0.1122054606657524 No Hit CTTATACACATCTCCGAGCCCACGAGACACCACTCGATCTCGTATGCCGT 9 0.1122054606657524 RNA PCR Primer, Index 32 (95% over 23bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.04986909362922329 0.0 4 0.0 0.0 0.0 0.04986909362922329 0.0 5 0.0 0.0 0.0 0.07480364044383493 0.0 6 0.0 0.0 0.0 0.07480364044383493 0.0 7 0.0 0.0 0.0 0.07480364044383493 0.0 8 0.0 0.0 0.0 0.08727091385114076 0.0 9 0.0 0.0 0.0 0.08727091385114076 0.0 10 0.0 0.0 0.0 0.09973818725844658 0.0 11 0.0 0.0 0.0 0.12467273407305822 0.0 12 0.0 0.0 0.0 0.12467273407305822 0.0 13 0.0 0.0 0.0 0.12467273407305822 0.0 14 0.0 0.0 0.0 0.14960728088766986 0.0 15 0.0 0.0 0.0 0.14960728088766986 0.0 16 0.0 0.0 0.0 0.17454182770228152 0.0 17 0.0 0.0 0.0 0.17454182770228152 0.0 18 0.0 0.0 0.0 0.2368781947388106 0.0 19 0.0 0.0 0.0 0.4612891160703154 0.0 20 0.0 0.0 0.0 0.5485600299214561 0.0 21 0.0 0.0 0.0 0.5859618501433737 0.0 22 0.0 0.0 0.0 0.5859618501433737 0.0 23 0.0 0.0 0.0 0.5984291235506795 0.0 24 0.0 0.0 0.0 0.635830943772597 0.0 25 0.0 0.0 0.0 0.6482982171799028 0.0 26 0.0 0.0 0.0 0.6607654905872086 0.0 27 0.0 0.0 0.0 0.6732327639945144 0.0 28 0.0 0.0 0.0 0.6981673108091261 0.0 29 0.0 0.0 0.0 0.6981673108091261 0.0 30 0.0 0.0 0.0 0.7106345842164319 0.0 31 0.0 0.0 0.0 0.7231018576237377 0.0 32 0.0 0.0 0.0 0.7480364044383493 0.0 33 0.0 0.0 0.0 0.7854382246602668 0.0 34 0.0 0.0 0.0 0.8228400448821843 0.0 35 0.0 0.0 0.0 0.910110958733325 0.0 36 0.0 0.0 0.0 1.1843909736940532 0.0 37 0.0 0.0 0.0 1.333998254581723 0.0 38 0.0 0.0 0.0 1.5584091759132277 0.0 39 0.0 0.0 0.0 1.683081909986286 0.0 40 0.0 0.0 0.0 1.8825582845031792 0.0 41 0.0 0.0 0.0 2.0072310185762374 0.0 42 0.0 0.0 0.0 2.169305572871213 0.0 43 0.0 0.0 0.0 2.4061837676100235 0.0 44 0.0 0.0 0.0 2.543323775090388 0.0 45 0.0 0.0 0.0 2.7178656027926693 0.0 46 0.0 0.0 0.0 2.830071063458422 0.0 47 0.0 0.0 0.0 2.992145617753397 0.0 48 0.0 0.0 0.0 3.079416531604538 0.0 49 0.0 0.0 0.0 3.2664256327141254 0.0 50 0.0 0.0 0.0 3.378631093379878 0.0 51 0.0 0.0 0.0 3.590574741304077 0.0 52 0.0 0.0 0.0 3.6404438349333 0.0 53 0.0 0.0 0.0 3.7526492955990527 0.0 54 0.0 0.0 0.0 3.7900511158209698 0.0 55 0.0 0.0 0.0 3.9022565764867223 0.0 56 0.0 0.0 0.0 3.9521256701159455 0.0 57 0.0 0.0 0.0 4.0269293105597805 0.0 58 0.0 0.0 0.0 4.051863857374392 0.0 59 0.0 0.0 0.0 4.139134771225533 0.0 60 0.0 0.0 0.0 4.301209325520508 0.0 61 0.0 0.0 0.0 4.351078419149732 0.0 62 0.0 0.0 0.0 4.400947512778956 0.0 63 0.0 0.0 0.0 4.413414786186261 0.0 64 0.0 0.0 0.0 4.4508166064081784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 35 3.1268428E-9 70.00001 1 TTACACA 35 3.1268428E-9 70.00001 3 AAAACCT 15 0.0021447965 70.00001 68 TTTACAC 35 3.1268428E-9 70.00001 2 AACACCA 15 0.0021447965 70.00001 70 TCGATAT 40 1.09139364E-10 70.0 34 GAAAAAC 20 7.458006E-5 70.0 60 CTCGATA 40 1.09139364E-10 70.0 33 ATCGTAT 65 0.0 64.61539 39 TATCGTA 55 0.0 63.63636 38 CGATATC 45 3.092282E-10 62.222225 35 GATATCG 45 3.092282E-10 62.222225 36 ATATCGT 45 3.092282E-10 62.222225 37 CTTATAC 620 0.0 53.629032 1 TTATACA 625 0.0 53.199997 2 TATACAC 625 0.0 52.639996 3 TTCTGCC 20 0.0067036054 52.5 52 CCTGAAA 20 0.0067036054 52.5 57 CATCACC 20 0.0067036054 52.5 28 TGCCTGA 20 0.0067036054 52.5 55 >>END_MODULE