FastQCFastQC Report
Wed 25 May 2016
SRR1780705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61640
Sequences flagged as poor quality0
Sequence length50
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCCTGTCTCTTATACACATCTGACGCTGCCGACG1240.2011680726800779No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG870.14114211550940947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC551.8189894E-1243.9674842
AATGTTA207.8268774E-443.9674835
CTTTACA553.3105607E-936.0026631
TTCTACA250.002344014135.1739852
ATTTCGG250.002344014135.17398542
CAGATGT250.00235339335.145415
TCAGATG250.00235339335.145414
TGCCGGC459.962387E-734.30850219
AGATTTC353.1965852E-431.40534240
TAGATTT353.1965852E-431.40534239
GACGCCT300.00563336429.40728414
TCCCAGA300.00570116329.3355031
GATTTCG300.005723905729.31165141
GTCAGAT300.005723905729.3116513
TCTACAC300.005723905729.3116513
GTAGATT300.005723905729.31165138
TATAACA300.005723905729.3116513
CTATACA406.9529435E-427.5020351
ACGAGAA857.908966E-925.88426824
TTGCGTG609.6131025E-625.64769632
Produced by FastQC (version 0.11.5)