FastQCFastQC Report
Wed 25 May 2016
SRR1780705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61640
Sequences flagged as poor quality0
Sequence length50
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT1565325.394224529526284No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTT26474.294289422452953TruSeq Adapter, Index 3 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG23723.848150551589877No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC20463.319273199221285No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG19053.090525632706035No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT14252.311810512654121No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT13492.188513951979234No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGT13462.1836469824789098No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGC9301.508760545100584No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCG4590.744646333549643No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCCT3420.554834523036989No Hit
CTTTACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC3280.5321219987021415No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGTCTT3220.5223880597014926No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCCT3060.49643088903309535No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGGCTT2960.48020765736534715No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGT2920.47371836469824785No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGG2860.46398442569759896No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGG2810.4558728098637248No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCG2790.4526281635301752No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGC2740.44451654769630106No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCC2700.4380272550292018No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGTCTT2670.4331602855288773No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT2670.4331602855288773No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCC2560.4153147306943543No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCC2470.4007138221933809No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT2250.3650227125243348No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT2190.35528877352368593No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCG2140.34717715768981183No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCC2060.33419857235561323No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC1940.3147306943543154No Hit
TATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCT1730.28066190785204415No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGC1690.27417261518494485No Hit
CTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC1500.24334847501622323No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGCCTT1480.24010382868267358No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCTTATGCCGT1460.23685918234912393No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCTCTT1360.22063595068137573No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCCT1270.20603504218040233No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG1240.2011680726800779No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGTCGT1180.19143413367942894No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCCCGTATGCCGT1150.18656716417910446No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCC1030.16709928617780662No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCCC950.15412070084360804No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGTCTT920.1492537313432836No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCCG910.14763140817650877No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGG860.13951979234263467No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCG840.13627514600908502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCC810.13140817650876055No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGG810.13140817650876055No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGC800.12978585334198572No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGC720.11680726800778714No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGCCTT700.11356262167423752No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGG680.11031797534068787No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCC650.10545100584036339No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG650.10545100584036339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA207.75106E-444.054471
GCCGCCT351.4117177E-744.01868443
CCCTCTT207.782194E-444.01868444
TTGCCCT302.481478E-644.01868444
ATGTCTT207.813427E-443.98295237
CTTTACA1100.042.0521
TTTACAC1100.042.0178342
ATGCCCC700.040.87449344
CCGCCTT557.4578566E-1140.01698744
CCGTCTT3700.039.8547544
TCTTTAT452.2646418E-839.1595271
CTTTATA452.2810127E-839.127722
TGCCGCC452.2810127E-839.1277242
TTTGCCC404.0270425E-738.51634643
CATGTCT357.1977465E-637.69967736
GCCGTCT3450.037.00121343
TTACACA1250.036.9756933
CTGATGC301.2836275E-436.6822441
ATCCCGG301.2897873E-436.65246237
ATGCCCT2350.036.52614244
Produced by FastQC (version 0.11.5)