FastQCFastQC Report
Wed 25 May 2016
SRR1780704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97462
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG150.00221981569.9917945
GGTGACG150.00221981569.9917910
GTGACGG150.00221981569.9917911
CCTGCAG150.00221981569.9917969
ACGACGG150.00221981569.9917970
AGGGAAA252.3815368E-455.99342716
TCAAAAG200.006936271752.49384336
TCCGATC200.006936271752.49384360
GAGTCGA200.006936271752.4938439
CGCGAGC200.006936271752.4938438
CGTTCAG200.006936271752.49384321
GGTGGCG200.006936271752.49384335
GATCTAG200.006936271752.49384370
TGTGTAC200.006936271752.49384322
TGAGAAC200.006936271752.49384358
TGCGTTC200.006936271752.49384319
AGTCGGT200.006936271752.49384369
GCGAGCC200.006936271752.4938439
GTACATG305.622782E-447.047421
GACTTTC305.856194E-446.66119463
Produced by FastQC (version 0.11.5)