##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780704_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 97462 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.005119944183374 34.0 31.0 34.0 12.0 34.0 2 31.46403726580616 34.0 33.0 34.0 23.0 34.0 3 31.59449836859494 34.0 34.0 34.0 23.0 34.0 4 31.87196035377891 34.0 34.0 34.0 23.0 34.0 5 31.900699759906423 34.0 34.0 34.0 27.0 34.0 6 34.54356569740001 38.0 36.0 38.0 24.0 38.0 7 34.045781945784 38.0 34.0 38.0 23.0 38.0 8 32.91934292339578 37.0 34.0 38.0 12.0 38.0 9 33.713970573146455 37.0 34.0 38.0 21.0 38.0 10-11 34.095806570766044 37.5 34.0 38.0 23.0 38.0 12-13 34.44751800701812 38.0 35.0 38.0 25.0 38.0 14-15 34.03359770987667 38.0 34.0 38.0 22.5 38.0 16-17 33.89935564630318 37.5 34.0 38.0 22.0 38.0 18-19 33.897975621267776 37.5 34.0 38.0 22.0 38.0 20-21 33.984445219675365 38.0 34.0 38.0 22.5 38.0 22-23 33.99061685580021 37.5 34.0 38.0 22.5 38.0 24-25 34.3868533377111 38.0 35.0 38.0 24.0 38.0 26-27 34.418440007387495 38.0 35.5 38.0 24.0 38.0 28-29 34.570114506166504 38.0 36.0 38.0 25.5 38.0 30-31 34.410036732264885 38.0 35.0 38.0 24.0 38.0 32-33 34.12101126592928 38.0 34.0 38.0 23.0 38.0 34-35 34.021162093944305 37.0 34.0 38.0 23.0 38.0 36-37 34.01297428741458 37.5 34.0 38.0 22.5 38.0 38-39 34.53430567811044 38.0 35.5 38.0 25.0 38.0 40-41 34.06488169748209 37.5 34.5 38.0 23.0 38.0 42-43 34.38059448810819 38.0 35.0 38.0 24.0 38.0 44-45 33.45371016396134 37.0 34.0 38.0 21.5 38.0 46-47 34.1891865547598 37.0 34.0 38.0 24.0 38.0 48-49 33.9039574398227 37.0 34.0 38.0 23.0 38.0 50-51 33.83991709589378 37.0 34.0 38.0 22.0 38.0 52-53 33.86034557058136 37.0 34.0 38.0 23.0 38.0 54-55 33.61567072294844 37.0 34.0 38.0 21.5 38.0 56-57 33.664069073074636 37.0 34.0 38.0 22.0 38.0 58-59 33.56918593913525 37.0 33.5 38.0 22.0 38.0 60-61 33.57498307032485 37.0 34.0 38.0 21.5 38.0 62-63 33.68550306786234 37.0 34.0 38.0 22.5 38.0 64-65 33.661401366686505 37.0 34.0 38.0 22.0 38.0 66-67 33.60321971640229 37.0 34.0 38.0 22.0 38.0 68-69 33.169219798485564 37.0 32.5 38.0 20.5 38.0 70-71 33.02825716689581 37.0 32.5 38.0 20.0 38.0 72-73 33.14118836059182 37.0 32.0 38.0 20.5 38.0 74-75 32.71511973897519 37.0 31.5 38.0 19.5 38.0 76 31.991955839198866 36.0 30.0 38.0 18.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.3337814498002096 1101 2 -1.2197010069303857 1101 3 -0.5123510601653649 1101 4 -2.759389991156066 1101 5 -0.6764951553500289 1101 6 -2.7992785809375995 1101 7 -2.0406956950237287 1101 8 -1.9000137689910908 1101 9 -1.6032185324993335 1101 10-11 -1.717666586651731 1101 12-13 -1.9191787447129443 1101 14-15 -0.8989622767822922 1101 16-17 -2.4582636207395225 1101 18-19 -0.5471995207637619 1101 20-21 -1.5775764940956591 1101 22-23 -1.2963010239668336 1101 24-25 -1.8437681567511461 1101 26-27 -2.257009470159197 1101 28-29 -1.3243805383153102 1101 30-31 -1.9818886065552448 1101 32-33 -0.37183773697532274 1101 34-35 -1.6867018534310745 1101 36-37 -0.8447404279366566 1101 38-39 -0.9920539025954156 1101 40-41 -0.3852643260602022 1101 42-43 -0.34740120539159136 1101 44-45 -0.3022550454056798 1101 46-47 0.09575673299313792 1101 48-49 -1.6810211634176895 1101 50-51 -1.353883540156879 1101 52-53 -0.5732307055855799 1101 54-55 -1.66997418593132 1101 56-57 -0.968725840673244 1101 58-59 -1.0597646962643523 1101 60-61 -0.6689126657992475 1101 62-63 -2.556418904702845 1101 64-65 -1.1158404653600584 1101 66-67 -1.551799379255776 1101 68-69 -0.16785131053211444 1101 70-71 -0.5631781174740667 1101 72-73 -1.204281345452177 1101 74-75 -2.6511974193916785 1101 76 -2.8810395233710935 1102 1 0.9203724184615432 1102 2 -0.44083948118089467 1102 3 0.2751505115685724 1102 4 0.02793195384222713 1102 5 0.0769312246323679 1102 6 1.1902132550272668 1102 7 1.768792189152954 1102 8 1.5562381395429767 1102 9 1.4536333293317867 1102 10-11 -0.2057842780889274 1102 12-13 -3.556817730899649 1102 14-15 -1.0222490867910992 1102 16-17 -2.9698226011781728 1102 18-19 -0.6866796809908138 1102 20-21 0.327966786966293 1102 22-23 -0.3137607426713629 1102 24-25 0.7555474653860514 1102 26-27 -5.75897638291449 1102 28-29 0.0729268569174053 1102 30-31 0.8044184473866665 1102 32-33 -6.239363019438365 1102 34-35 -1.073339420566569 1102 36-37 0.6641331328041247 1102 38-39 0.7981420701731707 1102 40-41 0.5353945448061381 1102 42-43 0.5799038546935478 1102 44-45 -5.556498727035255 1102 46-47 0.1516386104517693 1102 48-49 0.14311528105070437 1102 50-51 -5.651911238599517 1102 52-53 -1.4453544324810892 1102 54-55 0.7152712740029372 1102 56-57 -0.7744864700853284 1102 58-59 1.2773228358439255 1102 60-61 0.3646236457453682 1102 62-63 -3.9585133524404483 1102 64-65 -0.1706715264151839 1102 66-67 0.48997281811808335 1102 68-69 -7.091375227831961 1102 70-71 0.07410161524134651 1102 72-73 0.5735383452305811 1102 74-75 -5.695035324225433 1102 76 -1.2840321047869097 1103 1 -0.030895446914204427 1103 2 -0.3343234215528028 1103 3 0.4664368186225154 1103 4 0.3261799944139163 1103 5 0.6847843059294298 1103 6 -0.6858586675176852 1103 7 1.0494437951157636 1103 8 2.0104720414947224 1103 9 0.48542985614906087 1103 10-11 0.7686970497118963 1103 12-13 -0.667759793293996 1103 14-15 -0.47017439799441973 1103 16-17 -0.2646128270887331 1103 18-19 -0.23274545630970067 1103 20-21 -0.44842786494702835 1103 22-23 -0.27633950400531404 1103 24-25 0.6534420404590549 1103 26-27 0.3659583028085791 1103 28-29 0.9710980571632888 1103 30-31 0.6773706527040133 1103 32-33 -4.158826673964256 1103 34-35 -0.7009635176927418 1103 36-37 0.6172605340643145 1103 38-39 1.1572245966830863 1103 40-41 1.1081700173741424 1103 42-43 0.9284285204381391 1103 44-45 -3.9739722171228493 1103 46-47 0.05176923900563679 1103 48-49 0.31029677790025545 1103 50-51 0.7155007792274333 1103 52-53 0.3048837725289033 1103 54-55 0.6792562132990767 1103 56-57 1.7642948423474394 1103 58-59 1.206637420137767 1103 60-61 1.3782013313147488 1103 62-63 1.1364382381542981 1103 64-65 1.6756939261743398 1103 66-67 0.37528094782454957 1103 68-69 -3.222925865606662 1103 70-71 0.7026708483749005 1103 72-73 1.370802829631991 1103 74-75 -0.9688020369962942 1103 76 1.2290614867299183 1104 1 -1.6561184801664375 1104 2 -0.20096305388586444 1104 3 0.18760470183419287 1104 4 -0.1696026495439078 1104 5 -0.028408448869161163 1104 6 -0.45033555316245355 1104 7 0.869422167648878 1104 8 1.6905477376288118 1104 9 0.09172885587871349 1104 10-11 -0.5557555050326286 1104 12-13 -0.5866432753161632 1104 14-15 0.009022682297555207 1104 16-17 -0.6587328595299269 1104 18-19 0.766511099516201 1104 20-21 0.871928988256542 1104 22-23 0.4245255946700013 1104 24-25 0.37601570901811954 1104 26-27 1.1674857426571776 1104 28-29 -0.12846939349905284 1104 30-31 -0.5421075846574297 1104 32-33 -1.0514598180572534 1104 34-35 0.3650408078006393 1104 36-37 0.2610831888358689 1104 38-39 0.1159026177698621 1104 40-41 0.7018845348112848 1104 42-43 0.7484609616384574 1104 44-45 -2.963834342021471 1104 46-47 0.03046618412594171 1104 48-49 1.0376014548399652 1104 50-51 1.3326270295653657 1104 52-53 0.03376995798443261 1104 54-55 0.18998884373973368 1104 56-57 0.5303262021744146 1104 58-59 0.905650666961229 1104 60-61 0.5688744862214818 1104 62-63 1.0213530800034434 1104 64-65 0.48791344496319056 1104 66-67 0.4870273657753472 1104 68-69 -3.2061645787723876 1104 70-71 -0.17809422326608626 1104 72-73 0.592998017520614 1104 74-75 -0.8967420207756973 1104 76 0.8484451039237513 1105 1 -1.3704914065101654 1105 2 -0.5255018727312546 1105 3 -0.8481086359229408 1105 4 -0.45220384396991875 1105 5 -0.09710121595609067 1105 6 0.0038383021792824934 1105 7 1.0079623136342803 1105 8 0.17929359031627 1105 9 -0.4639977532785551 1105 10-11 0.6132425042573573 1105 12-13 -1.9008237663579663 1105 14-15 -0.4874471252671455 1105 16-17 0.2822558597799585 1105 18-19 0.1604195268552857 1105 20-21 -0.6458016023207662 1105 22-23 0.3901924825266718 1105 24-25 0.04610197311898645 1105 26-27 0.203769750620026 1105 28-29 -1.3774541313889017 1105 30-31 -0.5400704247370598 1105 32-33 0.16807568293809538 1105 34-35 0.13987823314900538 1105 36-37 0.9745669313707097 1105 38-39 -0.06105823159974477 1105 40-41 0.22261446181858702 1105 42-43 0.5606507426603571 1105 44-45 1.2122904091397757 1105 46-47 0.8388736161100212 1105 48-49 0.23793987554335416 1105 50-51 -0.3263510726244121 1105 52-53 -0.9223216146764841 1105 54-55 -1.3891949651521003 1105 56-57 -0.15028428223168788 1105 58-59 -0.057773354273010114 1105 60-61 -0.29886937575594885 1105 62-63 -0.021440549724488278 1105 64-65 -0.1836326731522675 1105 66-67 0.2485469410905452 1105 68-69 1.2634041007233066 1105 70-71 1.2970816227856687 1105 72-73 0.34299138182054634 1105 74-75 -1.3722363804306354 1105 76 -0.5477061900377578 1106 1 0.23915286226453958 1106 2 0.6733914079011569 1106 3 0.0067530241561115645 1106 4 0.5379937844885809 1106 5 0.6779185468897602 1106 6 0.4119015432860067 1106 7 1.4857488748991017 1106 8 1.2221394401186387 1106 9 2.0337097566028675 1106 10-11 1.1934006070241523 1106 12-13 0.42676516644836227 1106 14-15 0.5292327166300836 1106 16-17 1.1586194961435936 1106 18-19 0.8126724912821572 1106 20-21 -0.30437867741958513 1106 22-23 0.1350936682974293 1106 24-25 0.7426237122494186 1106 26-27 0.49539030397970407 1106 28-29 -0.91350156221894 1106 30-31 0.024040247199693 1106 32-33 1.3781152086297936 1106 34-35 0.9137122252438701 1106 36-37 0.8149621882877085 1106 38-39 0.7639220055544058 1106 40-41 0.8255393630043599 1106 42-43 0.5176467900911845 1106 44-45 1.2629623458986714 1106 46-47 1.0875692682839286 1106 48-49 0.6524062787054064 1106 50-51 0.7243208641344765 1106 52-53 0.4662949861140291 1106 54-55 0.177287248286639 1106 56-57 0.8782532671754311 1106 58-59 0.1853887010629549 1106 60-61 1.2693519681175687 1106 62-63 1.0693104384178085 1106 64-65 0.9728100145947707 1106 66-67 1.5254639371379781 1106 68-69 0.9924159584308114 1106 70-71 0.54862312476196 1106 72-73 0.6277344648245062 1106 74-75 -0.8039359851737231 1106 76 -0.5990895892472459 1107 1 -0.8750965823562176 1107 2 -0.13891925826542817 1107 3 0.07811565073930282 1107 4 -0.4214274670621556 1107 5 -0.4809631933947074 1107 6 0.3963797753047018 1107 7 0.055553554510190395 1107 8 1.409525839818592 1107 9 -0.43016895434026736 1107 10-11 -0.5831277678063458 1107 12-13 -0.09394254538915447 1107 14-15 -0.4946269527389475 1107 16-17 0.09491677550656874 1107 18-19 0.5457081798081731 1107 20-21 0.260615119643397 1107 22-23 -1.002481704600072 1107 24-25 -0.31194049536144064 1107 26-27 0.2952229689345529 1107 28-29 -0.8711701234260545 1107 30-31 -0.3651002853873635 1107 32-33 0.9540146344974829 1107 34-35 0.09679263261815407 1107 36-37 -0.3283328695582881 1107 38-39 0.11042816521512577 1107 40-41 0.22925679758500905 1107 42-43 -0.20774341792358797 1107 44-45 -0.18828689676599453 1107 46-47 0.18375085565878635 1107 48-49 0.24745546943850627 1107 50-51 0.9913861536529609 1107 52-53 0.5209962353566979 1107 54-55 0.15714212841127306 1107 56-57 0.3934648883058074 1107 58-59 0.5771835136765588 1107 60-61 -0.292439382391656 1107 62-63 -0.4184279418943646 1107 64-65 0.2433878975179411 1107 66-67 0.7187791905928691 1107 68-69 0.9106237423955008 1107 70-71 0.015336433668224458 1107 72-73 0.09664765255710961 1107 74-75 -0.03907778719905508 1107 76 -1.2354965019156623 1108 1 -0.11616386139610313 1108 2 0.03400783104811467 1108 3 -0.17288902407411655 1108 4 0.051398471317789074 1108 5 0.09231655728190802 1108 6 -0.25289986601956116 1108 7 0.9979248604507589 1108 8 0.7660692116986283 1108 9 0.12458924025226281 1108 10-11 0.13592551413137954 1108 12-13 0.7272048342549056 1108 14-15 1.032260058934419 1108 16-17 -0.4355241878877294 1108 18-19 0.04825745671568882 1108 20-21 0.5259791263138922 1108 22-23 -0.26801657433406945 1108 24-25 -0.5955800153045772 1108 26-27 -0.3544120675617961 1108 28-29 0.7340610201262479 1108 30-31 0.8327181350177923 1108 32-33 0.8414022066016997 1108 34-35 0.7131230407758409 1108 36-37 0.15450564434312497 1108 38-39 0.8767626740863363 1108 40-41 0.5243373082605274 1108 42-43 0.9901638512745876 1108 44-45 0.39743851945642916 1108 46-47 0.559738444165859 1108 48-49 0.8553862493942219 1108 50-51 0.7110272045291453 1108 52-53 0.32019796312399507 1108 54-55 0.32268109885880136 1108 56-57 0.9635665860011997 1108 58-59 -0.7250937492338565 1108 60-61 1.3962412813771792 1108 62-63 0.07707494227414458 1108 64-65 0.33145755497167073 1108 66-67 0.16895552127440538 1108 68-69 1.7417016832905432 1108 70-71 2.10575033235326 1108 72-73 1.0864915520622915 1108 74-75 1.1737697482721217 1108 76 1.3974759686275462 1109 1 -0.21728745690172246 1109 2 0.03775314940017438 1109 3 0.523740189409029 1109 4 0.14128611176722572 1109 5 0.49681093930437825 1109 6 0.810770545965461 1109 7 -0.7211762631447485 1109 8 1.5301141555188522 1109 9 1.7463120866942106 1109 10-11 1.368135251959096 1109 12-13 0.7833846095358012 1109 14-15 0.9180278491965979 1109 16-17 0.124400905745226 1109 18-19 0.9864597039066965 1109 20-21 1.1420839952277575 1109 22-23 1.33123436199552 1109 24-25 1.1628469509875572 1109 26-27 0.8347265092172336 1109 28-29 0.4194542785532178 1109 30-31 0.49938480168445665 1109 32-33 0.9537617571635053 1109 34-35 0.5071305314125425 1109 36-37 0.7668651949049234 1109 38-39 0.8655267190301572 1109 40-41 0.7602923644403106 1109 42-43 0.984545873746498 1109 44-45 0.46110893144144427 1109 46-47 0.11591821944675473 1109 48-49 0.3684948636264309 1109 50-51 1.108030949847496 1109 52-53 1.3052166897157562 1109 54-55 1.260883346049809 1109 56-57 1.4036414923682443 1109 58-59 0.6812732919646507 1109 60-61 0.8250802326880375 1109 62-63 0.42913486736778594 1109 64-65 0.8314575549716707 1109 66-67 0.9330004650946293 1109 68-69 1.9364582375976624 1109 70-71 0.802379545836402 1109 72-73 0.4048436119873813 1109 74-75 2.28800195800995 1109 76 0.3937306502754865 1110 1 0.12237370671595826 1110 2 0.8071794531630658 1110 3 0.18232129515681095 1110 4 0.14394974972880092 1110 5 0.2688062339946846 1110 6 0.24169849253044617 1110 7 1.0546049129122608 1110 8 1.7129857465355016 1110 9 -0.6283955215752357 1110 10-11 -0.6914112535371899 1110 12-13 0.2989202441441705 1110 14-15 0.5791035803449347 1110 16-17 0.7106378748767739 1110 18-19 1.1617257296777268 1110 20-21 0.23247867336022665 1110 22-23 0.7211869032683893 1110 24-25 0.39342064722465864 1110 26-27 -0.9266142336267791 1110 28-29 0.4043377950961684 1110 30-31 0.3502413573436769 1110 32-33 0.898420284185228 1110 34-35 0.9363075078454344 1110 36-37 0.06658531010388913 1110 38-39 0.424593228853162 1110 40-41 0.19683828853338525 1110 42-43 0.5970406343571071 1110 44-45 1.2827465955526378 1110 46-47 0.6511742396741766 1110 48-49 0.8833747294980654 1110 50-51 0.49271029921674625 1110 52-53 0.3125585953661769 1110 54-55 -0.5169665437540019 1110 56-57 0.23247909157861812 1110 58-59 -3.0341501183032022 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0.15004699323186088 2116 50-51 -0.3630952586709242 2116 52-53 0.556261894837256 2116 54-55 -0.006101237174654273 2116 56-57 -0.17050274593852066 2116 58-59 -1.0417691259431976 2116 60-61 -1.9618503482718097 2116 62-63 0.268608780789954 2116 64-65 0.20614181592454628 2116 66-67 -0.9109808954140348 2116 68-69 -0.18524283373758976 2116 70-71 -3.1791551636836743 2116 72-73 0.2694677736457791 2116 74-75 -1.913504879373555 2116 76 -1.4458739137868797 2117 1 0.028978597170365106 2117 2 0.4941816016890641 2117 3 0.7065177902824296 2117 4 0.12721463229066288 2117 5 -0.971550976721641 2117 6 -0.645642743091237 2117 7 -1.453090317944664 2117 8 -1.4104156478140233 2117 9 0.5300692323673815 2117 10-11 -0.14405598672534126 2117 12-13 0.7444909189567142 2117 14-15 -0.49385860852073193 2117 16-17 0.3479827644542297 2117 18-19 0.10145007525424887 2117 20-21 -0.05256641645400606 2117 22-23 0.1428387835940299 2117 24-25 0.8886047335164875 2117 26-27 1.0302033170535907 2117 28-29 0.5383795086552681 2117 30-31 0.40879965255406603 2117 32-33 1.2823414172038312 2117 34-35 0.20444209034445038 2117 36-37 -0.7717783023429519 2117 38-39 0.21887551886032242 2117 40-41 -0.884834930893966 2117 42-43 -0.25360026948539627 2117 44-45 0.9117677738802996 2117 46-47 0.3384613967504322 2117 48-49 0.0679141118333817 2117 50-51 0.7938108706954239 2117 52-53 0.6915834276496113 2117 54-55 -0.8264492897748497 2117 56-57 -0.5829011390590679 2117 58-59 -0.301137358689644 2117 60-61 1.1559116327092696 2117 62-63 0.20249761737157002 2117 64-65 -0.1427346238331637 2117 66-67 -0.48172909865918 2117 68-69 0.27542471169128646 2117 70-71 1.2098383397529204 2117 72-73 1.075991013767549 2117 74-75 -0.13137513641149923 2117 76 -1.0405291565449346 2118 1 -0.3071750973511591 2118 2 0.5059179434076633 2118 3 0.08928326057008107 2118 4 0.12630483835898687 2118 5 -0.5406422442162189 2118 6 0.24522747480440898 2118 7 0.6608462087653706 2118 8 -3.9343053201365734 2118 9 -1.482152332781446 2118 10-11 -0.8921643735090683 2118 12-13 0.5436842350039655 2118 14-15 -1.1793504279569618 2118 16-17 -0.03290246504128902 2118 18-19 -1.4685964758685799 2118 20-21 -2.9103504617010856 2118 22-23 -0.614458521899607 2118 24-25 -0.25850136361918885 2118 26-27 0.33098119086517386 2118 28-29 0.001851282298531487 2118 30-31 0.014366075092695496 2118 32-33 0.04739471596499811 2118 34-35 0.5108758497646022 2118 36-37 -0.3305130822486291 2118 38-39 -0.37979163302976815 2118 40-41 -1.5561870363087564 2118 42-43 -0.4199485082759722 2118 44-45 -0.3403891958993768 2118 46-47 -0.6687259753097976 2118 48-49 -1.1914302300065245 2118 50-51 -0.7178117467817131 2118 52-53 -1.7696896773254451 2118 54-55 -0.6585923093809072 2118 56-57 0.723866211469371 2118 58-59 0.7018725429009791 2118 60-61 -1.6955190182482909 2118 62-63 0.20254310706815915 2118 64-65 -0.18352371843656812 2118 66-67 -2.3291718195495648 2118 68-69 0.5057466271107742 2118 70-71 -0.025335809968836998 2118 72-73 -1.1944073243422046 2118 74-75 0.5651555160623829 2118 76 0.7570265304252999 2119 1 -0.34518774751355963 2119 2 0.2604053320971147 2119 3 0.2751505115685724 2119 4 -0.03845808765154857 2119 5 0.04788558147884103 2119 6 0.3852340019152294 2119 7 -2.0112907983989103 2119 8 0.6350763138168318 2119 9 -0.5878604466018231 2119 10-11 0.530730244732645 2119 12-13 0.15687521515844338 2119 14-15 -0.7234939000691085 2119 16-17 -0.3764616053275489 2119 18-19 -1.1514099714472437 2119 20-21 -1.6907054122038332 2119 22-23 0.19038863492199454 2119 24-25 -0.308767887311042 2119 26-27 -0.20088509660743625 2119 28-29 0.4878646162535034 2119 30-31 -0.20180561900338034 2119 32-33 -0.3472468368657502 2119 34-35 0.822926139599403 2119 36-37 -0.8171946680257491 2119 38-39 -0.40725212069819605 2119 40-41 -0.9334851232436563 2119 42-43 -1.41802145650977 2119 44-45 -1.429942710437743 2119 46-47 -0.23840288332415582 2119 48-49 -1.049830777040576 2119 50-51 0.39580660372413234 2119 52-53 -1.2752299511532854 2119 54-55 -1.7079652405198829 2119 56-57 0.05331435979019261 2119 58-59 0.6715137072132293 2119 60-61 -0.0689863127608561 2119 62-63 -0.464737418830488 2119 64-65 -0.3594682069131139 2119 66-67 0.1559479218525226 2119 68-69 -0.4544457672510447 2119 70-71 -0.9466452727254548 2119 72-73 -2.202395264727926 2119 74-75 -0.3029191416528221 2119 76 0.4296608412711791 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 19.0 13 122.0 14 310.0 15 558.0 16 685.0 17 867.0 18 881.0 19 917.0 20 888.0 21 780.0 22 758.0 23 779.0 24 863.0 25 1034.0 26 1197.0 27 1422.0 28 1761.0 29 2073.0 30 2696.0 31 3326.0 32 4213.0 33 5631.0 34 7773.0 35 11892.0 36 20137.0 37 25878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.703423233295055 23.17536182406919 12.567373245192059 28.553841697443694 2 17.076399006792393 21.63509880774045 37.35301963842318 23.935482547043975 3 16.78500338593503 27.008475097986906 29.481233711600417 26.72528780447764 4 12.14319427058751 18.84734563214381 39.1937370462334 29.815723051035274 5 11.994418337403296 37.02673862633642 37.21758223717962 13.761260799080668 6 26.451334879234984 39.43588270300219 18.716012394574296 15.396770023188525 7 25.2816482321315 31.46764892983932 23.479920379224723 19.770782458804455 8 22.107077630255894 35.66928649114527 20.968172210707763 21.255463667891075 9 23.45426935626193 15.974431060310684 22.449775297038848 38.12152428638854 10-11 24.20379224723482 25.92805401079395 27.849315630707355 22.01883811126388 12-13 23.599454146231352 24.081693377931913 26.37386878988734 25.944983685949396 14-15 21.691531058258605 25.33808048264965 23.86109458045187 29.109293878639882 16-17 21.7951611910283 27.801604727996555 26.304098007428532 24.09913607354661 18-19 21.57610145492602 26.85713406250641 26.96640742032792 24.60035706223964 20-21 21.071802343477458 27.93396400648458 26.5046890069976 24.489544643040364 22-23 22.570335104963988 26.833535121380642 26.645256612833716 23.950873160821654 24-25 21.81362992756151 27.536373150561243 26.652951919722558 23.997045002154685 26-27 20.892245182737888 28.228437750097473 27.19521454515606 23.684102522008576 28-29 21.598674355133284 27.274219695881474 28.240750241119617 22.88635570786563 30-31 21.99216104738257 27.07824588044571 27.51533931173175 23.414253760439966 32-33 20.734747901746324 28.084792021505816 27.243438468326115 23.937021608421745 34-35 21.85518458476124 27.334243089614414 27.483532043257885 23.32704028236646 36-37 22.324085284521146 26.671933676715025 27.060290164371754 23.943690874392072 38-39 21.581744679977838 27.40350085161396 28.076070673698467 22.938683794709732 40-41 21.741807063265682 26.600110812419196 27.598961646590464 24.05912047772465 42-43 21.17184133303236 26.42773593810921 28.029385811906177 24.37103691695225 44-45 20.413084073792863 26.39490262871683 28.521372432332605 24.670640865157704 46-47 20.85325562783444 25.885986333134962 28.901520592641234 24.35923744638936 48-49 20.424370523896492 26.382077117235436 29.273460425601773 23.9200919332663 50-51 20.216597237897847 26.626274855841253 28.35207568077815 24.80505222548275 52-53 19.59430342082042 26.70271490427038 28.9158851655004 24.787096509408794 54-55 19.170548521475038 27.207527036178202 28.485974020643944 25.13595042170282 56-57 19.249040651741193 26.708871149781455 28.880999774271 25.16108842420636 58-59 19.604563830005542 26.412345324331532 28.546510434836144 25.43658041082678 60-61 19.7656522542119 26.324618825798773 28.149945619831318 25.759783300158013 62-63 19.831831893455913 26.199954854199586 28.151997701668343 25.816215550676162 64-65 20.54544335228089 26.41798854938335 27.040282366460776 25.996285731874984 66-67 20.132974903039134 26.798136709691985 26.99411052512774 26.07477786214114 68-69 20.33561798444522 26.48673329092364 26.81147524163264 26.3661734829985 70-71 21.270341261209495 26.35899119656892 26.699636781514847 25.671030760706735 72-73 21.81619502985779 26.177894974451583 25.633067246721797 26.37284274896883 74-75 22.206090578892287 26.314871437072913 25.90086392645339 25.578174057581414 76 22.09373909831524 25.985512302230614 25.97730397488252 25.943444624571626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 0.5 10 1.0 11 1.0 12 0.0 13 0.5 14 1.0 15 3.0 16 4.5 17 4.0 18 6.5 19 15.5 20 23.0 21 24.0 22 32.0 23 58.0 24 82.5 25 89.0 26 159.5 27 278.0 28 402.0 29 478.0 30 592.0 31 794.0 32 1160.0 33 1438.0 34 1646.0 35 2152.5 36 2638.0 37 2825.0 38 3322.0 39 4195.5 40 5143.5 41 6019.5 42 6324.0 43 6442.0 44 7014.5 45 7403.5 46 7338.0 47 7312.0 48 7088.0 49 6540.5 50 6191.0 51 5778.0 52 4887.0 53 3977.0 54 3545.0 55 3350.0 56 2727.0 57 2028.0 58 1757.0 59 1411.5 60 919.5 61 649.5 62 526.0 63 414.0 64 251.0 65 171.0 66 116.0 67 90.0 68 67.0 69 39.0 70 31.0 71 28.0 72 25.5 73 18.5 74 14.0 75 14.0 76 9.5 77 8.0 78 7.0 79 3.0 80 3.5 81 2.5 82 1.5 83 2.0 84 2.5 85 1.5 86 0.5 87 1.0 88 0.5 89 1.5 90 2.0 91 2.0 92 3.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.5 98 1.0 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8198066938909523 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 97462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.85532823048983 #Duplication Level Percentage of deduplicated Percentage of total 1 73.60979625437332 36.69840553241263 2 9.557522123893806 9.52986805113788 3 5.272689853879399 7.886150499681928 4 3.169376414900185 6.320412058032875 5 1.9962955340605062 4.976298454782377 6 1.3315497015846882 3.9830908456629253 7 0.9837415105988887 3.433132913340584 8 0.7367771146326405 2.9385811906178816 9 0.6174109899156205 2.770310479981942 >10 2.7063181724634697 20.9538076378486 >50 0.018522329697468615 0.5099423365003797 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011286450103630132 0.0 2 0.0 0.0 0.0 0.016416654696189283 0.0 3 0.0 0.0 0.0 0.018468736533212946 0.0 4 0.0 0.0 0.0 0.023598941125772096 0.0 5 0.0 0.0 0.0 0.023598941125772096 0.0 6 0.0 0.0 0.0 0.023598941125772096 0.0 7 0.0 0.0 0.0 0.023598941125772096 0.0 8 0.0 0.0 0.0 0.024624982044283927 0.0 9 0.0 0.0 0.0 0.035911432147914056 0.0 10 0.0 0.0 0.0 0.04001559582196138 0.0 11 0.0 0.0 0.0 0.04719788225154419 0.0 12 0.0 0.0 0.0 0.052328086844103346 0.0 13 0.0 0.0 0.0 0.054380168681127 0.0 14 0.0 0.0 0.0 0.0677187006217808 0.0 15 0.0 0.0 0.0 0.07695306888838727 0.0 16 0.0 0.0 0.0 0.07900515072541094 0.0 17 0.0 0.0 0.0 0.08516139623648192 0.0 18 0.0 0.0 0.0 0.09234368266606473 0.0 19 0.0 0.0 0.0 0.09644784634011204 0.0 20 0.0 0.0 0.0 0.09849992817713571 0.0 21 0.0 0.0 0.0 0.10978637828076583 0.0 22 0.0 0.0 0.0 0.11286450103630133 0.0 23 0.0 0.0 0.0 0.11491658287332499 0.0 24 0.0 0.0 0.0 0.12107282838439597 0.0 25 0.0 0.0 0.0 0.12722907389546695 0.0 26 0.0 0.0 0.0 0.13646344216207343 0.0 27 0.0 0.0 0.0 0.14774989226570356 0.0 28 0.0 0.0 0.0 0.19494777451724773 0.0 29 0.0 0.0 0.0 0.2205987974800435 0.0 30 0.0 0.0 0.0 0.2503539841168866 0.0 31 0.0 0.0 0.0 0.2893435390203361 0.0 32 0.0 0.0 0.0 0.3180726847386674 0.0 33 0.0 0.0 0.0 0.3827132626049127 0.0 34 0.0 0.0 0.0 0.435041349449016 0.0 35 0.0 0.0 0.0 0.525332950278057 0.0 36 0.0 0.0 0.0 0.6576922287660832 0.0 37 0.0 0.0 0.0 0.9419055631938602 0.0 38 0.0 0.0 0.0 1.3615562988651988 0.0 39 0.0 0.0 0.0 1.8632903080174836 0.0 40 0.0 0.0 0.0 2.4860971455541647 0.0 41 0.0 0.0 0.0 3.2104820340235167 0.0 42 0.0 0.0 0.0 3.983090845662925 0.0 43 0.0 0.0 0.0 4.829574603435185 0.0 44 0.0 0.0 0.0 5.492397036793827 0.0 45 0.0 0.0 0.0 6.281422503129424 0.0 46 0.0 0.0 0.0 7.081734419568653 0.0 47 0.0 0.0 0.0 7.855369272126572 0.0 48 0.0 0.0 0.0 8.523321910077774 0.0 49 0.0 0.0 0.0 9.260019289569268 0.0 50 0.0 0.0 0.0 9.940284418542612 0.0 51 0.0 0.0 0.0 10.807288994685107 0.0 52 0.0 0.0 0.0 11.550142619687673 0.0 53 0.0 0.0 0.0 12.337116004186248 0.0 54 0.0 0.0 0.0 13.011224887648519 0.0 55 0.0 0.0 0.0 13.628901520592642 0.0 56 0.0 0.0 0.0 14.271203135581047 0.0 57 0.0 0.0 0.0 14.888879768525168 0.0 58 0.0 0.0 0.0 15.436785619010486 0.0 59 0.0 0.0 0.0 16.01547269705116 0.0 60 0.0 0.0 0.0 16.556196261106894 0.0 61 0.0 0.0 0.0 17.15437811659929 0.0 62 0.0 0.0 0.0 17.7361433173955 0.0 63 0.0 0.0 0.0 18.29225749522891 0.0 64 0.0 0.0 0.0 18.924298701032196 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGGCG 15 0.002219815 69.99179 45 GGTGACG 15 0.002219815 69.99179 10 GTGACGG 15 0.002219815 69.99179 11 CCTGCAG 15 0.002219815 69.99179 69 ACGACGG 15 0.002219815 69.99179 70 AGGGAAA 25 2.3815368E-4 55.993427 16 TCAAAAG 20 0.0069362717 52.493843 36 TCCGATC 20 0.0069362717 52.493843 60 GAGTCGA 20 0.0069362717 52.493843 9 CGCGAGC 20 0.0069362717 52.493843 8 CGTTCAG 20 0.0069362717 52.493843 21 GGTGGCG 20 0.0069362717 52.493843 35 GATCTAG 20 0.0069362717 52.493843 70 TGTGTAC 20 0.0069362717 52.493843 22 TGAGAAC 20 0.0069362717 52.493843 58 TGCGTTC 20 0.0069362717 52.493843 19 AGTCGGT 20 0.0069362717 52.493843 69 GCGAGCC 20 0.0069362717 52.493843 9 GTACATG 30 5.622782E-4 47.04742 1 GACTTTC 30 5.856194E-4 46.661194 63 >>END_MODULE