FastQCFastQC Report
Wed 25 May 2016
SRR1780704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97462
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT12691.3020459255915127No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT6110.6269110012107283RNA PCR Primer, Index 15 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1940.19905193819129505No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1200.12312491022141964No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTC207.892317E-570.036
CTAGAAC200.00693305552.53
GCGACTC200.00693305552.520
AACCAGG200.00693305552.57
TGACGTT200.00693305552.529
TTGACGT200.00693305552.528
CTTGACG200.00693305552.527
GGCGCTG200.00693305552.563
TGGCGTG352.0733072E-550.023
GCGTGAA352.0733072E-550.025
CGCCATT352.0733072E-550.017
CATATCG305.8528094E-446.66666458
AATTTAC305.8528094E-446.66666453
GGCACCG305.8528094E-446.66666467
ACCGATT305.8528094E-446.66666470
GCACCGA305.8528094E-446.66666468
GGGCACC305.8528094E-446.66666466
TTGGCGT404.5637687E-543.7522
ATTGGCG404.5637687E-543.7521
AGAATAT404.5637687E-543.755
Produced by FastQC (version 0.11.5)