##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780704_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 97462 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.07307463421641 34.0 34.0 34.0 32.0 34.0 2 33.39580554472512 34.0 34.0 34.0 33.0 34.0 3 33.523209045576735 34.0 34.0 34.0 34.0 34.0 4 33.526492376515975 34.0 34.0 34.0 34.0 34.0 5 33.537871170302274 34.0 34.0 34.0 34.0 34.0 6 37.29895754242679 38.0 38.0 38.0 36.0 38.0 7 36.89109601690915 38.0 37.0 38.0 35.0 38.0 8 36.9382938991607 38.0 37.0 38.0 35.0 38.0 9 36.75726949990766 38.0 37.0 38.0 34.0 38.0 10-11 36.4702858549999 38.0 37.0 38.0 34.0 38.0 12-13 36.35764708296567 38.0 37.0 38.0 34.0 38.0 14-15 36.57887176540601 38.0 37.0 38.0 34.0 38.0 16-17 36.719844657404934 38.0 37.0 38.0 34.0 38.0 18-19 36.72223533274507 38.0 37.0 38.0 34.0 38.0 20-21 36.4693880691962 38.0 37.0 38.0 34.0 38.0 22-23 36.540502965258256 38.0 37.0 38.0 34.0 38.0 24-25 35.91038558617718 38.0 36.5 38.0 32.5 38.0 26-27 36.12291970203772 38.0 37.0 38.0 33.5 38.0 28-29 35.97636514744208 38.0 37.0 38.0 33.0 38.0 30-31 35.72525702325009 38.0 36.5 38.0 31.5 38.0 32-33 36.221937780878704 38.0 37.0 38.0 34.0 38.0 34-35 36.2579261660955 38.0 37.0 38.0 34.0 38.0 36-37 36.38401633457143 38.0 37.0 38.0 34.0 38.0 38-39 36.02815456280396 38.0 36.5 38.0 32.5 38.0 40-41 35.594677925755676 38.0 36.0 38.0 31.0 38.0 42-43 35.987446389362006 38.0 36.5 38.0 32.5 38.0 44-45 36.16232993371776 38.0 37.0 38.0 34.0 38.0 46-47 36.46061028913833 38.0 37.0 38.0 34.0 38.0 48-49 36.261050460692374 38.0 37.0 38.0 34.0 38.0 50-51 36.400463770495165 38.0 37.0 38.0 34.0 38.0 52-53 36.33351460056227 38.0 37.0 38.0 34.0 38.0 54-55 36.48249574193019 38.0 37.0 38.0 34.0 38.0 56-57 36.52787240155138 38.0 37.0 38.0 34.0 38.0 58-59 36.5771480166629 38.0 37.0 38.0 34.0 38.0 60-61 36.28463914140896 38.0 37.0 38.0 34.0 38.0 62-63 36.55483675688986 38.0 37.0 38.0 34.0 38.0 64-65 36.543334838193346 38.0 37.0 38.0 34.0 38.0 66-67 35.991058053395165 38.0 37.0 38.0 33.0 38.0 68-69 35.41345857872812 38.0 36.0 38.0 30.0 38.0 70-71 35.407025302169046 38.0 36.0 38.0 31.0 38.0 72-73 35.13580164576963 38.0 36.0 38.0 28.5 38.0 74-75 35.72626767355482 38.0 36.5 38.0 31.5 38.0 76 35.202283967084604 38.0 36.0 38.0 28.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15045539987485768 1101 2 0.18680258041426612 1101 3 0.171529003866695 1101 4 0.010928140551207832 1101 5 0.11551715476216629 1101 6 -1.3804977770154707 1101 7 -0.0253754889293063 1101 8 0.2067337676857548 1101 9 0.10103333323650077 1101 10-11 -0.14722356200672948 1101 12-13 0.28184806478201097 1101 14-15 0.37096163320471476 1101 16-17 -0.2119291002941921 1101 18-19 -0.7731267163958933 1101 20-21 -0.20025039836317404 1101 22-23 0.41498551966949293 1101 24-25 -2.440690727248203 1101 26-27 -0.3216496222745775 1101 28-29 0.48544950644401297 1101 30-31 0.8683330652224655 1101 32-33 -0.008620874222202701 1101 34-35 0.1894046368283142 1101 36-37 0.1240787395130738 1101 38-39 0.4602133601741798 1101 40-41 0.9409277430930487 1101 42-43 0.4983475385980114 1101 44-45 -0.20320725504723214 1101 46-47 0.3740325000334579 1101 48-49 -1.6572115885429 1101 50-51 -1.6736047935860299 1101 52-53 -1.9304883296734445 1101 54-55 -1.2198190169276018 1101 56-57 -1.1693100939613785 1101 58-59 -1.273302342465719 1101 60-61 -1.9653813393642636 1101 62-63 -1.101688886750793 1101 64-65 -1.444463410575068 1101 66-67 -1.4263308928070302 1101 68-69 -1.5582201881248139 1101 70-71 -0.9371356597973204 1101 72-73 -2.2428328022793877 1101 74-75 -1.561897241034636 1101 76 -1.6774084688927928 1102 1 -1.154263486670498 1102 2 -0.2915254539303689 1102 3 0.2430922953469974 1102 4 0.4420143434216399 1102 5 -0.01913104627607254 1102 6 0.19191874145914056 1102 7 -0.6386032017420789 1102 8 0.40935272549143775 1102 9 0.1551369060122738 1102 10-11 0.3648383732917253 1102 12-13 0.3148993841403893 1102 14-15 -0.36385541696503054 1102 16-17 -0.22194329080630837 1102 18-19 -0.3775976258460574 1102 20-21 0.6340888446897566 1102 22-23 0.42264659994469156 1102 24-25 -4.380892986952542 1102 26-27 -1.3996345695541308 1102 28-29 0.09494996449219428 1102 30-31 1.5051008617412975 1102 32-33 0.3739571286877492 1102 34-35 0.6101982277463804 1102 36-37 -2.362754620764271 1102 38-39 -0.29036593604826066 1102 40-41 0.8771602519396708 1102 42-43 0.32534004816312745 1102 44-45 0.6208842827372507 1102 46-47 0.9173854126809786 1102 48-49 -2.7481206794519935 1102 50-51 -0.5918387035211836 1102 52-53 -0.12435048411651195 1102 54-55 0.44837447699202926 1102 56-57 0.5809907808208266 1102 58-59 0.022982976641181097 1102 60-61 -5.944032701833052 1102 62-63 -2.51871031622035 1102 64-65 -0.19079453449954542 1102 66-67 0.6239286678259788 1102 68-69 -0.8404048084837044 1102 70-71 -0.9693427334981664 1102 72-73 -5.847393525097374 1102 74-75 -1.9565820859269465 1102 76 0.048840385986764545 1103 1 0.6170289676095067 1103 2 0.34428213789382056 1103 3 -0.1704623381246435 1103 4 0.24455007417314079 1103 5 0.27419921344422704 1103 6 0.31103661451891895 1103 7 0.7365773730235574 1103 8 0.7046654656174525 1103 9 0.14196647416964225 1103 10-11 0.2008764860933212 1103 12-13 -0.5068003238663721 1103 14-15 -0.34738853514545553 1103 16-17 0.1893215009564102 1103 18-19 0.6591243658551846 1103 20-21 -0.01304501115778578 1103 22-23 0.5107286654126426 1103 24-25 -2.644009630567105 1103 26-27 -0.23812388874884505 1103 28-29 -0.2218857508912393 1103 30-31 0.8366591835485835 1103 32-33 1.289286773685447 1103 34-35 0.4638635112871867 1103 36-37 -0.31971635428201495 1103 38-39 0.1397708397316677 1103 40-41 0.06709416925947664 1103 42-43 -0.11928112903066079 1103 44-45 1.0777691759291983 1103 46-47 0.7873562133571781 1103 48-49 -1.6499725313038454 1103 50-51 -0.09378276376400407 1103 52-53 0.45854485935974765 1103 54-55 -0.0756150727236573 1103 56-57 0.42220024754896457 1103 58-59 0.04495162578825074 1103 60-61 -4.230508804491727 1103 62-63 -1.0500534851153844 1103 64-65 0.15228983617818415 1103 66-67 -0.31322362969977036 1103 68-69 0.06890823900361198 1103 70-71 0.7737109010492418 1103 72-73 -3.9775610370076215 1103 74-75 -1.7886168177542103 1103 76 -2.489625881110207 1104 1 0.16214927039404614 1104 2 0.29997286511739674 1104 3 0.394014547520122 1104 4 -0.27726663304503063 1104 5 -0.2411833223762656 1104 6 0.006711121142330967 1104 7 0.31846436950909407 1104 8 0.47306232963475736 1104 9 0.8764622838479355 1104 10-11 -0.8203995344527684 1104 12-13 0.2754651532896162 1104 14-15 0.5403378773984855 1104 16-17 0.001108470407615414 1104 18-19 0.4678834899427784 1104 20-21 -0.30946296195529754 1104 22-23 0.035383977659201094 1104 24-25 0.1570041569430245 1104 26-27 0.7773803015728689 1104 28-29 1.0974032461349807 1104 30-31 0.25166323869862595 1104 32-33 0.29216593021423876 1104 34-35 -0.20742602642570773 1104 36-37 0.9059932969750761 1104 38-39 0.830011445300741 1104 40-41 1.1176348559315485 1104 42-43 -0.061278966283978775 1104 44-45 0.37747179825956323 1104 46-47 0.7640931692912076 1104 48-49 -0.3284126502549114 1104 50-51 0.3670309696774723 1104 52-53 0.3353899526281978 1104 54-55 -0.03825362368337437 1104 56-57 -0.17465025258620415 1104 58-59 -0.8510743271720074 1104 60-61 0.08233250397001513 1104 62-63 0.2218172907699909 1104 64-65 0.4470586926258733 1104 66-67 0.046968314068820405 1104 68-69 1.368502723999562 1104 70-71 1.1012724041581876 1104 72-73 0.9777647267123015 1104 74-75 0.05940967589271651 1104 76 -1.0996285845435665 1105 1 0.7904969810775242 1105 2 0.24636967998136328 1105 3 0.17256796490566018 1105 4 -0.2417125520894885 1105 5 -0.14041358116856628 1105 6 0.2929221364044423 1105 7 0.6449275413737254 1105 8 -0.3319675310155432 1105 9 0.6122021644053319 1105 10-11 0.269832715049553 1105 12-13 -0.5907060477721018 1105 14-15 0.1618707241138111 1105 16-17 -0.03236200072709039 1105 18-19 0.5673061840370082 1105 20-21 0.0755071773943996 1105 22-23 0.20234482702880285 1105 24-25 0.3513439047864253 1105 26-27 0.2270949664700126 1105 28-29 0.6116832726777801 1105 30-31 0.7844369613263567 1105 32-33 0.901508995907669 1105 34-35 -0.8421970947734252 1105 36-37 0.27412202955636644 1105 38-39 0.013979593940419477 1105 40-41 0.6241312062965108 1105 42-43 0.43341247366294056 1105 44-45 0.6955806237406534 1105 46-47 0.8324740584750216 1105 48-49 -0.37539340672471866 1105 50-51 0.48059434061310924 1105 52-53 0.34379738461227305 1105 54-55 0.48909873199013987 1105 56-57 0.33294098828970675 1105 58-59 0.2880829389195654 1105 60-61 0.6245753705924457 1105 62-63 -0.4391780642399681 1105 64-65 0.5416837755721247 1105 66-67 0.5940154275392899 1105 68-69 0.1693122794076558 1105 70-71 1.181219318557659 1105 72-73 0.9590719596253763 1105 74-75 -0.1528929120303033 1105 76 0.6306434791591542 1106 1 -1.0461828608197123 1106 2 -0.04169356112535638 1106 3 0.20766677913490383 1106 4 0.19971037281170112 1106 5 -0.03148077484445366 1106 6 0.0348984209894212 1106 7 -0.47333332819148666 1106 8 0.07799294329275597 1106 9 -0.9255053454760898 1106 10-11 0.3017694739428336 1106 12-13 0.9736417783148568 1106 14-15 -0.6152043747004257 1106 16-17 0.1445549953203411 1106 18-19 0.4257251563690261 1106 20-21 -0.06322800047121291 1106 22-23 0.32511162544777505 1106 24-25 -0.6189327750554767 1106 26-27 0.721640421015465 1106 28-29 0.704252442638257 1106 30-31 0.505543680693954 1106 32-33 -0.0357242056733611 1106 34-35 0.6277633795348834 1106 36-37 0.8469283536670389 1106 38-39 0.006232558367301522 1106 40-41 0.5167794276403868 1106 42-43 0.5401318412518776 1106 44-45 0.8949482126734551 1106 46-47 0.6716044932576253 1106 48-49 0.9751994786507723 1106 50-51 0.11474453824155972 1106 52-53 0.5130463964699814 1106 54-55 0.16941493752374015 1106 56-57 0.13428486180749388 1106 58-59 0.5041303697495962 1106 60-61 0.6418085721734812 1106 62-63 0.6081737144161679 1106 64-65 -0.8492253153369731 1106 66-67 0.15093242358672398 1106 68-69 1.397454571897775 1106 70-71 0.10643670986200959 1106 72-73 0.4676885604158869 1106 74-75 -0.1498889594611299 1106 76 0.013726483111724974 1107 1 0.6439010952115609 1107 2 -0.21097603999209724 1107 3 0.029051043870488513 1107 4 0.2117844618454825 1107 5 0.058086750616432425 1107 6 0.19284250800364333 1107 7 0.6505811578302669 1107 8 -0.052485115620719114 1107 9 -0.32791727819586214 1107 10-11 0.4715712684669384 1107 12-13 0.36123157664727756 1107 14-15 0.1297539357926425 1107 16-17 0.5905245288017795 1107 18-19 0.6485404242493544 1107 20-21 0.5135297387746292 1107 22-23 -0.20731675226780766 1107 24-25 -0.14226591604967354 1107 26-27 -0.08028393200378048 1107 28-29 0.5590820782517696 1107 30-31 0.4651668883336626 1107 32-33 0.8888812586813941 1107 34-35 0.3728659443772102 1107 36-37 0.8621976765371215 1107 38-39 0.3482596204832191 1107 40-41 0.5446421552016218 1107 42-43 0.7106188439350092 1107 44-45 0.7917053749018947 1107 46-47 0.9629982787802618 1107 48-49 0.6314413643436225 1107 50-51 0.9929433784365926 1107 52-53 0.7094775438690775 1107 54-55 0.44897265368888384 1107 56-57 0.7322840539831361 1107 58-59 0.4372468407112038 1107 60-61 0.9801427229481234 1107 62-63 0.7747369987991846 1107 64-65 0.47625577291783827 1107 66-67 0.14550846005421647 1107 68-69 0.6367508991820401 1107 70-71 -0.152377230878308 1107 72-73 0.8117063325517151 1107 74-75 -1.0227071124284421 1107 76 0.5098604665513236 1108 1 -0.4242527635598634 1108 2 0.20703702761500153 1108 3 -0.06559342426696446 1108 4 0.3278209855339398 1108 5 -0.11751947153288711 1108 6 -0.22533458814442042 1108 7 0.06961940381840037 1108 8 -0.599287925976391 1108 9 -0.07971475762394675 1108 10-11 0.45601589516531504 1108 12-13 1.1495459100079444 1108 14-15 0.596702184787965 1108 16-17 -0.2538328893889883 1108 18-19 0.18365620106118286 1108 20-21 0.9524564453549047 1108 22-23 0.16684601872101013 1108 24-25 0.9112962370983055 1108 26-27 -0.22898947343465892 1108 28-29 -0.606675596917043 1108 30-31 0.19514857181325596 1108 32-33 0.6967149884170283 1108 34-35 0.6874590169051658 1108 36-37 1.098323595780407 1108 38-39 0.9980313474303699 1108 40-41 0.8779139778320939 1108 42-43 -0.10540264380385622 1108 44-45 0.7244400040607033 1108 46-47 0.7111597922169324 1108 48-49 0.9578718299113049 1108 50-51 0.49396172229509006 1108 52-53 -0.04545014688245175 1108 54-55 -0.3515618059737662 1108 56-57 0.3043267560799663 1108 58-59 0.46399032741121715 1108 60-61 0.9650384281341573 1108 62-63 0.845098408351113 1108 64-65 0.7161475140808022 1108 66-67 0.7339881888603657 1108 68-69 1.718471285195875 1108 70-71 -0.20920628580052636 1108 72-73 0.39343355308808725 1108 74-75 -0.33503795799557423 1108 76 1.3094585966259586 1109 1 -1.008522426481214 1109 2 0.14336661562998643 1109 3 0.3126837292910878 1109 4 0.21920675332420103 1109 5 -0.012650557675208063 1109 6 -0.3639113671706369 1109 7 -0.20753415423403254 1109 8 0.36325889050301186 1109 9 0.6169144558591952 1109 10-11 0.5440308764387183 1109 12-13 0.404227557948019 1109 14-15 0.8195486267355321 1109 16-17 0.7742569982514595 1109 18-19 0.6574389725967578 1109 20-21 0.935602512770636 1109 22-23 0.32040407115546543 1109 24-25 -0.025033350916672248 1109 26-27 0.2953550958537292 1109 28-29 -1.1759639864301548 1109 30-31 0.5041373358581964 1109 32-33 0.8502730408514907 1109 34-35 0.20805826784149417 1109 36-37 0.886713108889019 1109 38-39 1.089791647055847 1109 40-41 0.7505731538620566 1109 42-43 1.0668820003908976 1109 44-45 0.8105823261580838 1109 46-47 0.756103612441656 1109 48-49 0.61517520069782 1109 50-51 0.28422389457973907 1109 52-53 -0.3769108210397505 1109 54-55 -0.7523108696441767 1109 56-57 -0.24623504167283983 1109 58-59 0.04451467198050807 1109 60-61 1.0193455442390231 1109 62-63 0.26457406378182213 1109 64-65 0.5888066901107649 1109 66-67 0.26769605402890306 1109 68-69 -0.3583077410213562 1109 70-71 0.4555877216901081 1109 72-73 0.3391264369832214 1109 74-75 -0.7189330890817232 1109 76 -0.07630919363621302 1110 1 -1.031399966740004 1110 2 -0.49505467184010143 1110 3 0.10270252348787068 1110 4 0.2798890232304956 1110 5 0.21778792851308992 1110 6 -0.0397723171564337 1110 7 0.27995215574852494 1110 8 0.5050131056762837 1110 9 0.7320052494069742 1110 10-11 -0.2754839899061423 1110 12-13 0.4267603125823456 1110 14-15 0.8680079082293304 1110 16-17 0.6144184390497429 1110 18-19 0.2333120914869582 1110 20-21 0.9959535180573482 1110 22-23 -0.14966042403781188 1110 24-25 -0.20281428359559328 1110 26-27 1.2413319208513727 1110 28-29 0.6742300399898866 1110 30-31 0.23014093244549372 1110 32-33 0.8434584543914241 1110 34-35 0.9182558097228082 1110 36-37 1.3038496304753053 1110 38-39 1.0115050287943745 1110 40-41 -0.3720420789020409 1110 42-43 0.48247712742073645 1110 44-45 0.9801424878693012 1110 46-47 0.8901373338278304 1110 48-49 1.177872100331399 1110 50-51 0.06446700224749691 1110 52-53 0.6835348312811291 1110 54-55 0.2201062803984115 1110 56-57 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-3.7066546555310396 2116 40-41 -1.0978067781996117 2116 42-43 -1.1800477095646826 2116 44-45 -0.2646942176969844 2116 46-47 -0.4718599795799463 2116 48-49 0.18211187868754308 2116 50-51 -0.930173805968991 2116 52-53 -0.06768253082113063 2116 54-55 -0.7586038754799063 2116 56-57 0.09123556193311799 2116 58-59 -2.474299020206587 2116 60-61 -1.2330497174977566 2116 62-63 0.44228070954439147 2116 64-65 -1.3508109390156164 2116 66-67 -0.6836801327144144 2116 68-69 -1.2286369806346258 2116 70-71 0.7300847167253792 2116 72-73 0.046556103388589065 2116 74-75 -0.22884076262931785 2116 76 -1.154656027535701 2117 1 0.7917778055162401 2117 2 0.421445130846287 2117 3 0.058957364979271176 2117 4 -0.3581939654130082 2117 5 0.1506574530603615 2117 6 0.4596795894776875 2117 7 0.7921929075864611 2117 8 0.4461259544463658 2117 9 -0.6169329110455308 2117 10-11 -0.4397293018808881 2117 12-13 -0.9297123046730889 2117 14-15 -0.013837777910481464 2117 16-17 -0.5312063143082497 2117 18-19 -1.136219763699465 2117 20-21 0.22129297054861752 2117 22-23 0.31601431543513314 2117 24-25 -1.4947068865275241 2117 26-27 -2.2049330055579617 2117 28-29 -1.1031198189674214 2117 30-31 -0.19305291721614992 2117 32-33 -1.782215700448603 2117 34-35 0.23255446945182712 2117 36-37 -1.2148805468146335 2117 38-39 0.2228717544115284 2117 40-41 0.28469800791594935 2117 42-43 0.35336094624299363 2117 44-45 -0.2964473482873231 2117 46-47 -0.9858181756066884 2117 48-49 -0.6104688575896446 2117 50-51 -0.2856331161406729 2117 52-53 0.16362808023244924 2117 54-55 -0.39287403208788874 2117 56-57 0.028325603674318245 2117 58-59 -1.0828261519895293 2117 60-61 -0.2603933449025817 2117 62-63 -0.3191118147000367 2117 64-65 -0.6140394485721572 2117 66-67 -0.3146706232520273 2117 68-69 0.14578633167118227 2117 70-71 -0.5327593343683574 2117 72-73 0.5720715915723744 2117 74-75 0.6701251225666809 2117 76 1.0733670713564294 2118 1 0.07012925891204702 2118 2 0.1995463909108821 2118 3 -0.612409903667718 2118 4 0.2416786634365593 2118 5 0.38809850599520246 2118 6 0.24283020586307202 2118 7 0.6014546098109079 2118 8 0.42692930248803407 2118 9 -1.5740967800958572 2118 10-11 -0.9316245542728936 2118 12-13 -1.1126263746362497 2118 14-15 0.413181585537032 2118 16-17 -2.8811737133590327 2118 18-19 -0.7320741360174665 2118 20-21 -0.20859224378367003 2118 22-23 0.46085350935771174 2118 24-25 0.7371389337275218 2118 26-27 -1.8113864771799726 2118 28-29 -0.2209078066548713 2118 30-31 -0.4602821397972363 2118 32-33 -0.2733224647664869 2118 34-35 -0.5709679493869686 2118 36-37 -2.81366142294619 2118 38-39 -0.3502832593111975 2118 40-41 -0.5134717899581744 2118 42-43 0.39085203784408407 2118 44-45 -2.0002790970628865 2118 46-47 0.016403237909820234 2118 48-49 -0.306173113908919 2118 50-51 -0.899296704671201 2118 52-53 -1.1315924689798251 2118 54-55 -0.7373295962359379 2118 56-57 0.18260390963801854 2118 58-59 -0.8187812081693053 2118 60-61 -0.4750364782328802 2118 62-63 -1.154901591648887 2118 64-65 -0.0853506132600188 2118 66-67 0.41001815140718634 2118 68-69 -0.8751616736056249 2118 70-71 0.008022178618951159 2118 72-73 1.002047785297826 2118 74-75 0.35784593713551516 2118 76 0.09223013220648113 2119 1 0.7544502062755569 2119 2 -0.49899433359841794 2119 3 0.05222092605239226 2119 4 -0.14304789724226197 2119 5 -0.18925552760387632 2119 6 -0.8537244120678338 2119 7 -0.8834164797503732 2119 8 0.2682738873171644 2119 9 -0.08967637199602763 2119 10-11 0.06608318656973466 2119 12-13 -0.02949895610857567 2119 14-15 -0.2704944211144067 2119 16-17 -0.2613623779432359 2119 18-19 -0.17635281256804802 2119 20-21 -1.3949567984637667 2119 22-23 -0.2122081718395421 2119 24-25 1.0693144819271296 2119 26-27 0.7870874221471169 2119 28-29 0.39785452880754235 2119 30-31 1.0984618575919214 2119 32-33 0.32416459756741745 2119 34-35 0.4089534144683711 2119 36-37 0.0231374954183039 2119 38-39 -0.8069634464217046 2119 40-41 -0.6933791671474694 2119 42-43 0.10749772451167416 2119 44-45 0.1644527474675428 2119 46-47 0.16842275707931975 2119 48-49 0.07138139523680564 2119 50-51 0.8044268566447883 2119 52-53 0.28021383123618904 2119 54-55 0.2637271740874638 2119 56-57 -0.8671419992621594 2119 58-59 -0.0039879777156741625 2119 60-61 0.3630212400756605 2119 62-63 -0.07887629132408591 2119 64-65 0.2780851335502419 2119 66-67 0.36666725703759084 2119 68-69 0.5745329508523938 2119 70-71 0.11571950716572843 2119 72-73 0.8662994209607149 2119 74-75 0.21976646179089698 2119 76 0.33099806233531126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 5.0 22 3.0 23 9.0 24 28.0 25 76.0 26 139.0 27 238.0 28 406.0 29 715.0 30 1226.0 31 2015.0 32 3154.0 33 5079.0 34 7825.0 35 12446.0 36 22411.0 37 41685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.745603414664174 23.247009090722536 12.048798506084422 27.958588988528867 2 16.77269089491289 19.866204264226056 39.6010752908826 23.760029549978455 3 17.24364367650982 25.829554082616813 30.376967433461243 26.54983480741212 4 12.505386714822187 19.508115983665427 39.448195194024336 28.538302107488043 5 11.892840286470625 37.534628880999776 37.7624099649094 12.810120867620201 6 26.973589706757505 40.854897293304056 17.551455952063368 14.620057047875068 7 25.348340891834763 30.42929552030535 24.175576121975745 20.04678746588414 8 22.349223287024685 37.52334243089614 20.63983911678398 19.48759516529519 9 23.268555950011287 14.847838131784696 22.975108247316903 38.908497670887115 10-11 24.292544786686094 26.505715047916112 27.8139172190187 21.3878229463791 12-13 24.102727216761405 23.17159508321192 26.879706962713673 25.845970737313007 14-15 21.764379963472944 24.889700601259978 23.78824567523753 29.55767376002955 16-17 22.688842831052103 28.254601793519523 25.18366132441362 23.872894051014754 18-19 22.461574767601732 26.965381379409408 26.573433748537887 23.999610104450966 20-21 21.343703186883094 27.065420368964315 26.23791836818452 25.35295807596807 22-23 22.24354107241797 27.882661960558984 26.757095072951508 23.116701894071536 24-25 22.626767355482137 28.39465637889639 26.332827153146866 22.645749112474604 26-27 21.570458229874205 28.558309905399028 27.18905829964499 22.682173565081776 28-29 21.453489565163856 28.51572920728079 27.77031047998194 22.260470747573414 30-31 21.446307278734274 27.882148940099732 28.074531612320698 22.597012168845293 32-33 21.85159344154645 28.901520592641234 26.966920440787177 22.27996552502514 34-35 21.625864439473848 27.169563522193265 29.45199154542283 21.752580492910056 36-37 21.88801789415362 28.09710451252796 27.379388890028935 22.635488703289486 38-39 21.577127495844532 27.269602511748168 27.898565594795922 23.254704397611377 40-41 21.10514867332909 26.032197164022904 29.02977570745521 23.832878455192795 42-43 22.152223430670414 25.987051363608384 28.898955490344957 22.96176971537625 44-45 20.30534977734912 26.97615480905379 29.020028318729352 23.69846709486774 46-47 21.716669060762143 25.72694999076563 28.875869569678436 23.680511378793785 48-49 20.95996388335967 25.850074900987053 29.02721060515893 24.162750610494346 50-51 19.686647103486486 25.937288379060558 29.601280499066302 24.774784018386654 52-53 19.643040364449735 26.09222055775584 30.875110299398738 23.389628778395682 54-55 18.72473374238165 26.29486363916193 30.42160021341651 24.558802405039913 56-57 19.947774517247748 25.73823644086926 29.03644497342554 25.277544068457452 58-59 18.65496295992284 26.925878804046704 29.99989739590815 24.419260840122302 60-61 19.813363156922698 27.780570889167063 27.81186513718167 24.59420081672857 62-63 18.413330323613305 28.876895610596954 27.28704520736287 25.422728858426876 64-65 19.26340522460036 29.434035829348876 26.042970593667274 25.259588352383496 66-67 18.939689314809875 29.147770413084075 25.86597853522398 26.04656173688207 68-69 19.273665633785477 29.11545012415095 25.594077691818352 26.016806550245224 70-71 19.50657692228766 28.857400833145224 25.365270566990212 26.270751677576904 72-73 19.467587367384212 28.48751308202171 25.23496337033921 26.809936180254866 74-75 19.44757956947323 29.27910365065359 25.37655701709384 25.896759762779343 76 19.579938847961255 29.091338162565922 25.405799183271427 25.922923806201393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 1.5 16 2.0 17 4.0 18 3.0 19 4.0 20 9.0 21 12.0 22 18.5 23 50.0 24 80.5 25 86.0 26 171.0 27 265.0 28 380.0 29 486.0 30 590.0 31 814.5 32 1196.0 33 1457.0 34 1602.0 35 2191.5 36 3051.0 37 3466.0 38 4066.0 39 5146.5 40 5919.0 41 6371.5 42 6532.0 43 6676.0 44 7181.5 45 7592.0 46 7641.0 47 7531.5 48 7242.0 49 6469.0 50 5876.0 51 5384.5 52 4315.0 53 3416.5 54 3096.0 55 2981.0 56 2336.0 57 1573.0 58 1340.0 59 1071.0 60 683.5 61 463.0 62 361.0 63 282.5 64 156.0 65 83.5 66 48.0 67 37.0 68 26.5 69 10.0 70 2.0 71 0.0 72 0.5 73 1.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 97462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.50267796679732 #Duplication Level Percentage of deduplicated Percentage of total 1 56.89178338834865 19.629188812049826 2 14.057156451660868 9.700190843610844 3 8.472358521426235 8.769571730520614 4 5.5401909180123114 7.64605692475016 5 3.407975733785351 5.879214463072787 6 2.3403812412644602 4.844965217212863 7 1.7753590864483897 4.2878249984609385 8 1.263865346299105 3.4885391229402227 9 0.9902756713355338 3.075044632779955 >10 5.20117762512267 29.42069729740822 >50 0.047580813037142775 1.0075721819786172 >100 0.005947601629642847 0.3221768484127147 >500 0.0029738008148214234 0.6269110012107283 >1k 0.0029738008148214234 1.3020459255915127 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 1269 1.3020459255915127 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 611 0.6269110012107283 RNA PCR Primer, Index 15 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 194 0.19905193819129505 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 120 0.12312491022141964 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013338531940653793 0.0 2 0.0 0.0 0.0 0.018468736533212946 0.0 3 0.0 0.0 0.0 0.022572900207260264 0.0 4 0.0 0.0 0.0 0.026677063881307587 0.0 5 0.0 0.0 0.0 0.026677063881307587 0.0 6 0.0 0.0 0.0 0.026677063881307587 0.0 7 0.0 0.0 0.0 0.028729145718331246 0.0 8 0.0 0.0 0.0 0.029755186636843078 0.0 9 0.0 0.0 0.0 0.04309371857749687 0.0 10 0.0 0.0 0.0 0.04719788225154419 0.0 11 0.0 0.0 0.0 0.053354127762615174 0.0 12 0.0 0.0 0.0 0.059510373273686155 0.0 13 0.0 0.0 0.0 0.06156245511070982 0.0 14 0.0 0.0 0.0 0.07695306888838727 0.0 15 0.0 0.0 0.0 0.08618743715499375 0.0 16 0.0 0.0 0.0 0.08618743715499375 0.0 17 0.0 0.0 0.0 0.09234368266606473 0.0 18 0.0 0.0 0.0 0.09849992817713571 0.0 19 0.0 0.0 0.0 0.10363013276969485 0.0 20 0.0 0.0 0.0 0.10773429644374218 0.0 21 0.0 0.0 0.0 0.11799470562886048 0.0 22 0.0 0.0 0.0 0.12004678746588414 0.0 23 0.0 0.0 0.0 0.12312491022141964 0.0 24 0.0 0.0 0.0 0.12825511481397878 0.0 25 0.0 0.0 0.0 0.1354374012435616 0.0 26 0.0 0.0 0.0 0.14056760583612074 0.0 27 0.0 0.0 0.0 0.1528800968582627 0.0 28 0.0 0.0 0.0 0.19699985635427142 0.0 29 0.0 0.0 0.0 0.22675504299111449 0.0 30 0.0 0.0 0.0 0.2575362705464694 0.0 31 0.0 0.0 0.0 0.29549978453140713 0.0 32 0.0 0.0 0.0 0.32320288933122654 0.0 33 0.0 0.0 0.0 0.38476534444193633 0.0 34 0.0 0.0 0.0 0.43709343128603967 0.0 35 0.0 0.0 0.0 0.5314891957891281 0.0 36 0.0 0.0 0.0 0.6679526379512015 0.0 37 0.0 0.0 0.0 0.9644784634011204 0.0 38 0.0 0.0 0.0 1.3800250353984116 0.0 39 0.0 0.0 0.0 1.8961236174098623 0.0 40 0.0 0.0 0.0 2.545607518827851 0.0 41 0.0 0.0 0.0 3.304877798526605 0.0 42 0.0 0.0 0.0 4.104163674047321 0.0 43 0.0 0.0 0.0 5.010157805093267 0.0 44 0.0 0.0 0.0 5.726334366214525 0.0 45 0.0 0.0 0.0 6.56460979663869 0.0 46 0.0 0.0 0.0 7.405963349818391 0.0 47 0.0 0.0 0.0 8.229874207383391 0.0 48 0.0 0.0 0.0 8.932712236563994 0.0 49 0.0 0.0 0.0 9.721737702899592 0.0 50 0.0 0.0 0.0 10.43278405942829 0.0 51 0.0 0.0 0.0 11.335700067718701 0.0 52 0.0 0.0 0.0 12.108308879358109 0.0 53 0.0 0.0 0.0 12.931193696004597 0.0 54 0.0 0.0 0.0 13.652500461718413 0.0 55 0.0 0.0 0.0 14.31737497691408 0.0 56 0.0 0.0 0.0 14.993535942213375 0.0 57 0.0 0.0 0.0 15.651228170979458 0.0 58 0.0 0.0 0.0 16.21144651248692 0.0 59 0.0 0.0 0.0 16.833227309105087 0.0 60 0.0 0.0 0.0 17.41294042806427 0.0 61 0.0 0.0 0.0 18.036773306519464 0.0 62 0.0 0.0 0.0 18.65650202130061 0.0 63 0.0 0.0 0.0 19.25673595863003 0.0 64 0.0 0.0 0.0 19.933922964847838 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGGTC 20 7.892317E-5 70.0 36 CTAGAAC 20 0.006933055 52.5 3 GCGACTC 20 0.006933055 52.5 20 AACCAGG 20 0.006933055 52.5 7 TGACGTT 20 0.006933055 52.5 29 TTGACGT 20 0.006933055 52.5 28 CTTGACG 20 0.006933055 52.5 27 GGCGCTG 20 0.006933055 52.5 63 TGGCGTG 35 2.0733072E-5 50.0 23 GCGTGAA 35 2.0733072E-5 50.0 25 CGCCATT 35 2.0733072E-5 50.0 17 CATATCG 30 5.8528094E-4 46.666664 58 AATTTAC 30 5.8528094E-4 46.666664 53 GGCACCG 30 5.8528094E-4 46.666664 67 ACCGATT 30 5.8528094E-4 46.666664 70 GCACCGA 30 5.8528094E-4 46.666664 68 GGGCACC 30 5.8528094E-4 46.666664 66 TTGGCGT 40 4.5637687E-5 43.75 22 ATTGGCG 40 4.5637687E-5 43.75 21 AGAATAT 40 4.5637687E-5 43.75 5 >>END_MODULE