FastQCFastQC Report
Wed 25 May 2016
SRR1780703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636765
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGC301.3073503E-436.63366341
CCGAGTT250.002339688435.2375413
CGACAGT250.002359639935.1766127
GTTAAGC451.0492968E-634.167253
TTGCGAT803.6379788E-1233.06122616
CGAGTTC300.00570216529.3761814
TTATTGT300.005788287629.2862194
GTACAAT852.6739144E-1028.44272631
AACGATT407.0008705E-427.51849639
ACGACAG407.055805E-427.48172626
ATTTCGA407.055805E-427.48172627
GGTACAA905.16593E-1026.8731330
TGTTCGT508.306173E-526.41359710
GCTATAC508.4269785E-526.3575973
CATCGTT858.305506E-925.85702332
CGAATTT609.610207E-625.7122618
TGCGATT959.440555E-1025.51089517
CCAGATT450.00138310924.48786518
TTCAACG450.001392022224.46088436
ATGAACT450.001394577624.45318423
Produced by FastQC (version 0.11.5)