FastQCFastQC Report
Wed 25 May 2016
SRR1780703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636765
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT127992.0100036905294734No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT51410.8073622136895087Illumina PCR Primer Index 11 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC15630.24545947091941298No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC13680.21483592848225014No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG12320.19347797067992115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCAT250.002353463635.19541526
CCGTCTT6400.034.71422644
GCCGTCT7150.031.07287443
ATTAGCC300.005737839729.3387243
ACTGGAC300.005737839729.3387243
GACGGTG300.005746656629.3295127
ACTGTGC300.005746656629.3295128
CGTGCGA554.94308E-628.00954810
ATTCGGT554.94308E-628.00954811
ATGCCGT21600.028.00561144
CAATTCG554.9521514E-628.0029479
TTCGTAG554.961237E-627.99635135
TGCCGTC8000.027.77138142
CTGGCTA407.0144574E-427.50937810
TCGACAT407.0338085E-427.49641629
TTCGACA407.0338085E-427.49641628
ATGATTC1850.027.3477940
AGCGGAT1850.027.3477935
GATAATT655.9061676E-727.08615711
TCCACGA655.9061676E-727.08615712