FastQCFastQC Report
Wed 25 May 2016
SRR1780702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40546
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTTG150.00221047869.99135634
GAGTGGA150.00221047869.99135613
TTGGATG150.00221047869.99135620
GTGGGGC150.00221047869.99135637
ATGAGGG150.00221047869.9913567
TGACAAC150.00221047869.99135664
CTTGAGT150.00221047869.99135610
ACCGTTA150.00221047869.99135638
ACTGGGG150.00221047869.99135631
TTCTATG200.00690732252.4935245
AGACTGG200.00690732252.4935229
GGAGCAC200.00690732252.4935266
ATAGGCG200.00690732252.493523
CTTTTAG200.00690732252.4935221
TTTCGAA200.00690732252.4935222
ATGTGGT200.00690732252.493527
ATTAACT200.00690732252.4935252
GAATATC200.00690732252.4935226
GCTTGAG200.00690732252.493529
TTTTTAG200.00690732252.4935227
Produced by FastQC (version 0.11.5)