FastQCFastQC Report
Wed 25 May 2016
SRR1780702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40546
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT11662.875746066196419No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT4801.183840576135747Illumina PCR Primer Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1670.41187786711389535No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1280.3156908203028659No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1050.2589651260296947No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT710.17510975188674593No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT560.1381147338825038No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT480.1183840576135747No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC480.1183840576135747No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT440.10851871947911015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAAT150.002209396370.051
GTGTTGA150.002209396370.011
GCGAAGT150.002209396370.032
GGCGAAG150.002209396370.031
CGGGAGG150.002209396370.038
TTAAGGC150.002209396370.027
CCTCACG150.002209396370.033
ATTTTGT252.3635043E-456.00000438
TGTTGTC200.00690395152.557
GTCAAAA200.00690395152.561
TGTCAAA200.00690395152.560
GGCCTCA200.00690395152.531
ACGGGAG200.00690395152.537
AGTTGGA200.00690395152.533
TTTGGCC200.00690395152.528
TTGGCCT200.00690395152.529
CGGTTGT200.00690395152.555
CTCACGG200.00690395152.534
TTAAAAT200.00690395152.521
GTTGTCA200.00690395152.558