##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780702_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40546 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.038598135451096 34.0 34.0 34.0 32.0 34.0 2 33.38474818724412 34.0 34.0 34.0 33.0 34.0 3 33.5281655403739 34.0 34.0 34.0 34.0 34.0 4 33.533295516203815 34.0 34.0 34.0 34.0 34.0 5 33.54340748779165 34.0 34.0 34.0 34.0 34.0 6 37.27060622502836 38.0 38.0 38.0 36.0 38.0 7 36.890963350268834 38.0 37.0 38.0 35.0 38.0 8 36.943668919252204 38.0 37.0 38.0 35.0 38.0 9 36.75418043703448 38.0 37.0 38.0 34.0 38.0 10-11 36.48190943619593 38.0 37.0 38.0 34.0 38.0 12-13 36.33950328022493 38.0 37.0 38.0 34.0 38.0 14-15 36.56276821388053 38.0 37.0 38.0 34.0 38.0 16-17 36.68392689784443 38.0 37.0 38.0 34.0 38.0 18-19 36.669918117693484 38.0 37.0 38.0 34.0 38.0 20-21 36.39021111823608 38.0 37.0 38.0 34.0 38.0 22-23 36.5107408868939 38.0 37.0 38.0 34.0 38.0 24-25 35.836321708676564 38.0 36.5 38.0 32.5 38.0 26-27 36.06255857544517 38.0 37.0 38.0 33.0 38.0 28-29 35.94728209934395 38.0 37.0 38.0 32.5 38.0 30-31 35.68176885512751 38.0 36.5 38.0 31.5 38.0 32-33 36.16930153406008 38.0 37.0 38.0 34.0 38.0 34-35 36.10702658708627 38.0 37.0 38.0 33.0 38.0 36-37 36.255055985793916 38.0 37.0 38.0 34.0 38.0 38-39 35.6874907512455 38.0 36.5 38.0 32.5 38.0 40-41 35.43910620036502 38.0 36.0 38.0 30.5 38.0 42-43 35.89705519656687 38.0 36.5 38.0 32.5 38.0 44-45 36.08765352932471 38.0 37.0 38.0 33.5 38.0 46-47 36.37230552952202 38.0 37.0 38.0 34.0 38.0 48-49 36.18377891777241 38.0 37.0 38.0 34.0 38.0 50-51 36.30393873625019 38.0 37.0 38.0 34.0 38.0 52-53 36.18215113698022 38.0 37.0 38.0 34.0 38.0 54-55 36.385673062694224 38.0 37.0 38.0 34.0 38.0 56-57 36.423112020914516 38.0 37.0 38.0 34.0 38.0 58-59 36.469442115128494 38.0 37.0 38.0 34.0 38.0 60-61 36.17187885364771 38.0 37.0 38.0 33.0 38.0 62-63 36.470416317269276 38.0 37.0 38.0 34.0 38.0 64-65 36.45207912001184 38.0 37.0 38.0 34.0 38.0 66-67 35.79224831056084 38.0 36.5 38.0 31.5 38.0 68-69 35.103351748631184 38.0 36.0 38.0 29.0 38.0 70-71 34.908338677058154 38.0 36.0 38.0 26.0 38.0 72-73 34.43390223449909 38.0 35.5 38.0 24.0 38.0 74-75 34.93365560104573 38.0 36.0 38.0 26.0 38.0 76 34.43555467863661 37.0 35.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6380674503179051 1101 2 0.4964242123498508 1101 3 0.32537708258224995 1101 4 0.3881492301592431 1101 5 0.23460519564232385 1101 6 -0.6599872408256857 1101 7 -0.29780334916227247 1101 8 0.4509545628615541 1101 9 0.3349455333126201 1101 10-11 -0.006558878977749316 1101 12-13 0.6372256543250359 1101 14-15 0.8788840293816023 1101 16-17 -0.16524814895761608 1101 18-19 -0.02578757350934069 1101 20-21 0.3382573087489362 1101 22-23 0.7526144061185747 1101 24-25 -1.8416146455961595 1101 26-27 -0.823176773863814 1101 28-29 0.35626335824248656 1101 30-31 0.7165506593194877 1101 32-33 -0.07804241189671757 1101 34-35 0.2852909961684844 1101 36-37 0.1685966029006849 1101 38-39 -0.030532105971865064 1101 40-41 1.201150536212431 1101 42-43 0.37595077590725623 1101 44-45 0.11713919691644037 1101 46-47 0.5650945454122649 1101 48-49 -1.1913827969245432 1101 50-51 -1.4074941685524678 1101 52-53 -1.94392164289615 1101 54-55 -0.976288253068752 1101 56-57 -1.598834955224298 1101 58-59 -1.4503718278582198 1101 60-61 -1.1295207878288167 1101 62-63 -0.6846047803727799 1101 64-65 -0.7660740814255789 1101 66-67 -1.1787309008568911 1101 68-69 -1.262159902360196 1101 70-71 -1.0190942616963383 1101 72-73 -2.489794450586011 1101 74-75 -1.5433887167733928 1101 76 -1.241680197046172 1102 1 -1.065141105831831 1102 2 0.23468921532074205 1102 3 0.03571576350916672 1102 4 0.31298583146643466 1102 5 -0.02475309312772822 1102 6 0.136507114492197 1102 7 -0.30523056247184144 1102 8 0.46848278627213347 1102 9 0.3928777971272339 1102 10-11 0.49893725887132945 1102 12-13 0.5956332597914695 1102 14-15 0.3766558653887344 1102 16-17 0.40233949215943454 1102 18-19 0.06289335340687074 1102 20-21 0.48338505681785904 1102 22-23 0.858377923646799 1102 24-25 -4.108251603409592 1102 26-27 -0.919730546888168 1102 28-29 0.34660798094004974 1102 30-31 1.5871686034668429 1102 32-33 0.8365446350432677 1102 34-35 0.6417972350276671 1102 36-37 -1.9950991784421532 1102 38-39 0.43574834560269693 1102 40-41 1.5231945053152245 1102 42-43 0.8625817919500349 1102 44-45 1.4005616568094936 1102 46-47 1.1368414259826807 1102 48-49 -3.342897948439692 1102 50-51 -0.41492138186203675 1102 52-53 0.46724888592144254 1102 54-55 0.8277818598248885 1102 56-57 0.6579980810205015 1102 58-59 0.20069769085836242 1102 60-61 -4.716270639284552 1102 62-63 -1.7933391832248233 1102 64-65 -0.07564033216829813 1102 66-67 1.1753986297432277 1102 68-69 0.5038828783884668 1102 70-71 -0.7734020454158852 1102 72-73 -4.696568069124336 1102 74-75 -1.103994777379448 1102 76 0.12017363539589354 1103 1 0.8941682816399705 1103 2 0.3053989151298637 1103 3 -0.10717856236277612 1103 4 0.2249240580845182 1103 5 0.11553842170829398 1103 6 0.4001718724307679 1103 7 0.8066035517048036 1103 8 0.35664867209548845 1103 9 -0.13783537309660687 1103 10-11 -0.008480752581043305 1103 12-13 -1.0158538129137185 1103 14-15 -0.3930387381163811 1103 16-17 0.2020531829454839 1103 18-19 0.3403963900197482 1103 20-21 -0.42503937490172916 1103 22-23 0.45771854485103347 1103 24-25 -2.7966517422261816 1103 26-27 -0.5086599239487342 1103 28-29 -0.40564778862441386 1103 30-31 1.06534837094906 1103 32-33 1.0769142342103706 1103 34-35 0.14101639171688873 1103 36-37 -0.7569419686555818 1103 38-39 -0.1769967524365157 1103 40-41 -0.5469946322867187 1103 42-43 -0.9915740296706517 1103 44-45 1.132558880477717 1103 46-47 0.5605356824463286 1103 48-49 -1.9018949985871885 1103 50-51 -0.49482768066473426 1103 52-53 -0.27975788138725477 1103 54-55 -0.38274217279700906 1103 56-57 -0.2853907789482619 1103 58-59 -1.055896097099307 1103 60-61 -4.024443465937338 1103 62-63 -1.3535508785224124 1103 64-65 -0.23232955653060827 1103 66-67 -0.5483563589779266 1103 68-69 -0.3332245309469499 1103 70-71 -0.6935825069810448 1103 72-73 -4.661690401243028 1103 74-75 -3.1010449248720775 1103 76 -2.5610687730719164 1104 1 0.03622291803858957 1104 2 0.3713473568382142 1104 3 0.15515310366261303 1104 4 -0.36160922394703476 1104 5 -0.5835766225394963 1104 6 0.12806721679398692 1104 7 0.5685119780226202 1104 8 0.533870680560284 1104 9 0.5829033725748047 1104 10-11 -1.0646177283409415 1104 12-13 0.0013451097488399455 1104 14-15 -0.008467749274522873 1104 16-17 0.21244179394971496 1104 18-19 0.5531320575842003 1104 20-21 -0.17667461922646766 1104 22-23 -0.015194113863856273 1104 24-25 0.6927287887472744 1104 26-27 0.8287775520035652 1104 28-29 1.1888500951770524 1104 30-31 0.68277815163394 1104 32-33 0.29472877826577815 1104 34-35 -0.19784470870634863 1104 36-37 0.7043893126064447 1104 38-39 0.3925684649547847 1104 40-41 1.110065775562454 1104 42-43 0.4141589445502092 1104 44-45 0.5071260216858704 1104 46-47 0.5059377601685213 1104 48-49 -0.13275302090346486 1104 50-51 0.39070521660344326 1104 52-53 0.33732561226415214 1104 54-55 0.02441442589479692 1104 56-57 -0.05491752000251182 1104 58-59 -0.8067618146829858 1104 60-61 0.22823063948782618 1104 62-63 -0.022154187487402055 1104 64-65 0.5376589005682746 1104 66-67 -0.491950048858655 1104 68-69 1.3499613097610137 1104 70-71 1.1098034106795893 1104 72-73 0.2755546930239987 1104 74-75 -1.0547194150606032 1104 76 -1.9966209085086248 1105 1 1.0077644200148725 1105 2 0.5610706769963159 1105 3 0.3314376886428505 1105 4 0.2628967049067228 1105 5 -0.038122077084949524 1105 6 0.08142690058845403 1105 7 0.5072471558882299 1105 8 -0.5167222048152098 1105 9 0.795551593918681 1105 10-11 0.5727340503151837 1105 12-13 -0.1996430325436478 1105 14-15 -0.01202506152748839 1105 16-17 -0.2506016843111496 1105 18-19 0.46360636588459414 1105 20-21 0.24684316733479506 1105 22-23 0.14251339601756285 1105 24-25 0.6654560614745435 1105 26-27 0.1758131251260906 1105 28-29 0.527980529959656 1105 30-31 0.917560760329593 1105 32-33 0.6694323355780227 1105 34-35 -0.6662241553466615 1105 36-37 0.6514248857289715 1105 38-39 0.30936688392711886 1105 40-41 0.29761518267707743 1105 42-43 -0.07101892106243923 1105 44-45 0.6383513181285636 1105 46-47 0.8393369696546955 1105 48-49 -0.362594918136665 1105 50-51 0.2324048213465275 1105 52-53 0.011633912659412715 1105 54-55 0.3787622519817546 1105 56-57 0.4531852467959041 1105 58-59 0.21932514183875185 1105 60-61 0.5315903232822947 1105 62-63 -0.8482411440091369 1105 64-65 0.7147339993825028 1105 66-67 0.5722792001532042 1105 68-69 -0.00912959932988855 1105 70-71 0.5192895766874983 1105 72-73 0.17081161002004563 1105 74-75 -0.5600553834400586 1105 76 0.4855925302265547 1106 1 -1.4822445808852187 1106 2 0.2310976796965889 1106 3 0.33827837271125816 1106 4 0.28143855909213755 1106 5 -0.1479330581830638 1106 6 0.2789066485632503 1106 7 -0.5074243112584824 1106 8 -0.0025907916738958647 1106 9 -0.4676347247131858 1106 10-11 0.5334451214222895 1106 12-13 0.7428862203816493 1106 14-15 -0.46585044406574383 1106 16-17 -0.17089871401412182 1106 18-19 0.1048754927972837 1106 20-21 -0.20099661664360013 1106 22-23 0.23306245092305744 1106 24-25 -0.6052910132329217 1106 26-27 0.8494404878623669 1106 28-29 0.5477825101576741 1106 30-31 0.9116201662701897 1106 32-33 -0.054690076663192144 1106 34-35 0.13422588965783433 1106 36-37 0.5468344266830698 1106 38-39 0.3626072079595204 1106 40-41 1.1077411952783365 1106 42-43 0.6177749583254908 1106 44-45 0.7171992029170369 1106 46-47 0.6612541613738614 1106 48-49 1.2445607974348931 1106 50-51 -0.08906232536814684 1106 52-53 0.4203647857467203 1106 54-55 0.47430680643719825 1106 56-57 -0.27575264699347457 1106 58-59 0.10348355768033457 1106 60-61 0.42263442769273496 1106 62-63 0.3824519252977865 1106 64-65 -1.5570031743348665 1106 66-67 -0.11687471523833182 1106 68-69 0.43015932956298997 1106 70-71 0.1298206297928104 1106 72-73 0.6383183606951164 1106 74-75 0.3299986219604847 1106 76 0.3946834393174612 1107 1 0.8999167121671618 1107 2 0.3837590996847382 1107 3 0.17774693495209704 1107 4 0.31122615323617 1107 5 0.07454303558016306 1107 6 0.3866328057943562 1107 7 0.7915517401928156 1107 8 0.2264013383083352 1107 9 -0.7637335653664792 1107 10-11 0.4538140813952154 1107 12-13 -0.1187961016967165 1107 14-15 0.3373144878120655 1107 16-17 0.5216205379110761 1107 18-19 0.7030391553173843 1107 20-21 0.49482296531459014 1107 22-23 -0.2710841175799459 1107 24-25 -0.052570356551875363 1107 26-27 0.06493511424807963 1107 28-29 0.9596044615835879 1107 30-31 0.45858640135522677 1107 32-33 1.013643679789368 1107 34-35 -0.48238577150827666 1107 36-37 1.0901195244236064 1107 38-39 0.3871058116660535 1107 40-41 1.0010728361347319 1107 42-43 0.6094395093959903 1107 44-45 0.22358830336554547 1107 46-47 0.9703004506181756 1107 48-49 0.8451362395944955 1107 50-51 1.1690404079821093 1107 52-53 0.501688302713795 1107 54-55 0.7253434485629455 1107 56-57 0.7488728924835542 1107 58-59 0.39539351790712374 1107 60-61 0.9423517840437512 1107 62-63 0.8321007363327411 1107 64-65 0.38388706853557153 1107 66-67 -0.10841233053832866 1107 68-69 0.3730382328379349 1107 70-71 -0.9219691645712373 1107 72-73 0.4157999550083957 1107 74-75 -1.1851913585760343 1107 76 0.6339996786336997 1108 1 -0.30290751674402117 1108 2 -0.17647873011830484 1108 3 0.016022668880005142 1108 4 0.3514342543138369 1108 5 0.0946842470257181 1108 6 -0.7762806092929679 1108 7 -0.5619228045860751 1108 8 -0.7096075803092816 1108 9 -0.29535749699041247 1108 10-11 0.057121402093834206 1108 12-13 0.9665625010531897 1108 14-15 0.3045757289863502 1108 16-17 -0.5310364669198435 1108 18-19 0.3879146662798476 1108 20-21 0.9798471199039653 1108 22-23 -0.24562889647255304 1108 24-25 0.34055487570379483 1108 26-27 -0.27122244799643624 1108 28-29 -0.6937586004751282 1108 30-31 -0.2780914135834536 1108 32-33 0.403424430439685 1108 34-35 0.5543292043371295 1108 36-37 0.882650182171659 1108 38-39 0.9708293345200119 1108 40-41 -0.04210813748102282 1108 42-43 -0.49453670762370194 1108 44-45 0.6506042825554417 1108 46-47 0.9276768906033084 1108 48-49 0.7281165443139344 1108 50-51 0.42983565138604973 1108 52-53 -0.48876134425758977 1108 54-55 -0.7016725306269436 1108 56-57 -0.3201349113068588 1108 58-59 0.15410775053440062 1108 60-61 0.9064915090530477 1108 62-63 0.8343675516430338 1108 64-65 0.2246154223074086 1108 66-67 0.3863108207065693 1108 68-69 1.0760482662827542 1108 70-71 -0.11628354584214406 1108 72-73 -0.22009748088904502 1108 74-75 -0.785154197669307 1108 76 0.8816399610565924 1109 1 -0.9635521944051888 1109 2 0.3991584512909867 1109 3 0.3993060272008435 1109 4 0.43891551368415094 1109 5 0.07159579125360693 1109 6 0.004154173315725984 1109 7 -0.6767653832967255 1109 8 0.21070726226943037 1109 9 0.856366640940621 1109 10-11 0.39726383087944583 1109 12-13 0.6067049298387985 1109 14-15 0.7284138099458701 1109 16-17 0.8209500398267835 1109 18-19 0.736490378423774 1109 20-21 1.09731338677053 1109 22-23 0.5023096342620761 1109 24-25 0.2780736163334794 1109 26-27 0.10520183985963882 1109 28-29 -0.8125367114196536 1109 30-31 0.7485952430882108 1109 32-33 0.7176298277410353 1109 34-35 -0.3521175722745653 1109 36-37 1.0282273935659632 1109 38-39 1.1764515234255484 1109 40-41 0.7081167500751988 1109 42-43 1.1139340569939833 1109 44-45 0.9287901896019193 1109 46-47 0.8435689445763188 1109 48-49 0.7763549251235276 1109 50-51 -0.04220332912369429 1109 52-53 -0.36211216884529307 1109 54-55 -0.8031342997423891 1109 56-57 0.05302850698399908 1109 58-59 0.5494975556318522 1109 60-61 1.185352078768183 1109 62-63 0.2965864421977571 1109 64-65 1.0420067266552309 1109 66-67 0.09155819701841494 1109 68-69 0.46720268907136386 1109 70-71 0.6045560343677536 1109 72-73 1.135074932904061 1109 74-75 0.7264649927354938 1109 76 1.1102006806967708 1110 1 -0.5825499385237833 1110 2 -0.5053694249578058 1110 3 -0.42030402758484087 1110 4 0.06935379921428364 1110 5 0.22016169521751294 1110 6 -0.17107947154408976 1110 7 0.7051231117081116 1110 8 0.5048580840547956 1110 9 0.9214649329161304 1110 10-11 -0.5704690083084429 1110 12-13 0.4240188196228729 1110 14-15 0.9931576062719998 1110 16-17 0.4909871007355804 1110 18-19 -0.10253636988431225 1110 20-21 0.8115415530612182 1110 22-23 -0.4099929761149781 1110 24-25 -1.411944108448985 1110 26-27 0.4921257844294047 1110 28-29 0.6074197822961054 1110 30-31 0.2614859939744534 1110 32-33 0.5755495828167696 1110 34-35 1.0934190052310768 1110 36-37 1.4004478254061183 1110 38-39 0.9678630606475949 1110 40-41 -0.8805071622676977 1110 42-43 0.09274488522472524 1110 44-45 0.8547489391990837 1110 46-47 0.9048000112859569 1110 48-49 1.2817126434606223 1110 50-51 -0.19117206905279005 1110 52-53 0.3474028166526111 1110 54-55 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-1.5521149278593853 2116 44-45 0.43886108873179097 2116 46-47 0.09638879633268971 2116 48-49 0.26302105467556913 2116 50-51 -0.5790225363425137 2116 52-53 0.07170188207317807 2116 54-55 -0.0833872807285232 2116 56-57 0.4574758160397465 2116 58-59 -2.186282334796765 2116 60-61 -0.6709160488502519 2116 62-63 0.11998315505627488 2116 64-65 -1.2103297219428981 2116 66-67 0.03612796820758035 2116 68-69 -0.7369545780894526 2116 70-71 1.0151264500944706 2116 72-73 1.0686875658399302 2116 74-75 0.47388684255824387 2116 76 -0.5585875887199236 2117 1 0.7289765412269915 2117 2 0.44505336097900283 2117 3 0.4387969960021607 2117 4 0.13735557936559672 2117 5 0.1973757584128819 2117 6 0.31605893522048945 2117 7 0.344909493550567 2117 8 0.05643796834496584 2117 9 -1.1334527350856476 2117 10-11 -0.3815949540138206 2117 12-13 -1.0721538550544736 2117 14-15 0.32693597743355696 2117 16-17 -0.9386969224063932 2117 18-19 -1.4534932012149753 2117 20-21 -0.40358973309810864 2117 22-23 0.37238352588769885 2117 24-25 -1.2310807350622497 2117 26-27 -2.5583860090730397 2117 28-29 -1.4672575652784374 2117 30-31 -0.2681535253846974 2117 32-33 -1.3058923397466486 2117 34-35 0.7588840697615638 2117 36-37 -0.43039329608921406 2117 38-39 0.8325270570872974 2117 40-41 0.11255024761214827 2117 42-43 0.7785481785046571 2117 44-45 -0.35125907148182733 2117 46-47 0.03782181813954111 2117 48-49 -0.30956461510636046 2117 50-51 -0.19811465917295124 2117 52-53 0.9406382416637413 2117 54-55 0.28114320436270646 2117 56-57 0.3572978009084622 2117 58-59 -0.2901986676850612 2117 60-61 0.1257461674381375 2117 62-63 -0.1672887630567601 2117 64-65 -0.4675170828685822 2117 66-67 0.03310171097571413 2117 68-69 0.7832946430943508 2117 70-71 -0.04174938435146203 2117 72-73 1.1608549000633346 2117 74-75 1.1341004607157856 2117 76 1.0518262964603196 2118 1 -0.7773127669491089 2118 2 0.3238494076995764 2118 3 -0.40046625646863987 2118 4 0.32001505825149223 2118 5 0.40698941519634957 2118 6 0.6032107770893091 2118 7 0.6540739140357417 2118 8 0.5940839701419094 2118 9 -1.516112213971546 2118 10-11 -0.5571115757568563 2118 12-13 -0.3052176466088241 2118 14-15 0.8141327429322089 2118 16-17 -2.257205457896056 2118 18-19 -0.43673668728865067 2118 20-21 -0.51199045187618 2118 22-23 0.7558067064806835 2118 24-25 1.1870684113887862 2118 26-27 -1.3607220378241678 2118 28-29 0.2921314733558802 2118 30-31 0.1996362320277072 2118 32-33 0.6955901400377229 2118 34-35 -0.13123273167599336 2118 36-37 -2.9096385791080834 2118 38-39 -0.41756279017236153 2118 40-41 -0.36507778473287544 2118 42-43 0.8616568936888385 2118 44-45 -1.90530219816646 2118 46-47 0.17587213260495105 2118 48-49 0.2765989069062158 2118 50-51 -0.5389502386877822 2118 52-53 -1.335192845488102 2118 54-55 -1.2023698234899456 2118 56-57 0.31116123307378274 2118 58-59 -0.12218429212351367 2118 60-61 -0.4485125926696796 2118 62-63 -0.49635435155630603 2118 64-65 0.17408219835334648 2118 66-67 0.6242517558993512 2118 68-69 -1.1490952940125752 2118 70-71 -0.20643941130563803 2118 72-73 0.9374496889222783 2118 74-75 0.006068115702305477 2118 76 0.20600419403444192 2119 1 0.40490246715292244 2119 2 -0.41182494589930485 2119 3 -0.6249596177544561 2119 4 -0.7970359550259403 2119 5 -0.40765069661357245 2119 6 -0.07917916001760972 2119 7 -0.6244026757616012 2119 8 -0.10123398932699246 2119 9 0.008346206713291338 2119 10-11 0.009013511594645252 2119 12-13 0.20919535129473843 2119 14-15 -0.04409576859818998 2119 16-17 -0.6496757583852286 2119 18-19 -0.40878420650597747 2119 20-21 -1.4311029606113337 2119 22-23 -0.568092664588498 2119 24-25 0.6806917517102349 2119 26-27 0.6347356840486498 2119 28-29 0.14372127070039653 2119 30-31 1.0814496492184773 2119 32-33 0.49476903591472876 2119 34-35 0.47594756182505193 2119 36-37 -0.03131922201513504 2119 38-39 -0.5844041598439205 2119 40-41 -0.5391693291074304 2119 42-43 0.2686275435840244 2119 44-45 0.582206536983783 2119 46-47 0.6109699662876835 2119 48-49 -0.7086386891804324 2119 50-51 0.6177583567000582 2119 52-53 -0.01967921865372091 2119 54-55 -0.0962377480182468 2119 56-57 -0.5655857969751352 2119 58-59 -0.3047489322353272 2119 60-61 0.30061389230585434 2119 62-63 0.19249959673160078 2119 64-65 -0.17095623630773105 2119 66-67 -0.2262898234158115 2119 68-69 0.42218353198324365 2119 70-71 -0.20722557482765325 2119 72-73 0.5678654820739197 2119 74-75 0.03952374113906387 2119 76 0.17246121709523976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 11.0 24 16.0 25 40.0 26 59.0 27 128.0 28 210.0 29 372.0 30 593.0 31 973.0 32 1511.0 33 2346.0 34 3593.0 35 5267.0 36 9002.0 37 16425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.63473585557145 23.70887387165195 11.715089034676664 29.94130123809994 2 16.428254328417108 18.786070142554138 41.05707098110788 23.72860454792088 3 15.831401371282 25.514230750258964 32.787451289892964 25.866916588566074 4 11.752084052680905 22.10082375573423 39.50328022492971 26.643811966655157 5 11.1552310955458 37.87303309820944 38.68199082523554 12.289744981009223 6 25.265130962363735 41.996744438415625 17.503576185073744 15.234548414146895 7 24.091155724362455 30.858777684605137 23.639816504710698 21.410250086321707 8 21.171015636560945 38.884230256992055 20.773935776648745 19.17081832979825 9 21.632220194347163 14.460119370591427 22.840724115819068 41.066936319242345 10-11 23.00720169683816 26.599417945050067 28.888176392245846 21.50520396586593 12-13 22.843190450352687 22.395550732501356 27.73023232871307 27.03102648843289 14-15 20.643220046367087 24.48330291520742 23.368519706012925 31.50495733241257 16-17 22.696443545602527 28.964632762787947 24.59552113648695 23.74340255512258 18-19 23.145316430720662 27.208602574853252 24.996300498199574 24.64978049622651 20-21 20.44344694914418 26.555023923444978 25.145513737483355 27.856015389927492 22-23 21.90228382577813 28.32955162038179 25.71030434568145 24.057860208158637 24-25 23.029398707640706 29.0953484930696 25.511764415725345 22.36348838356435 26-27 21.976273861786613 28.61318009174764 27.557588911359936 21.852957135105804 28-29 21.739505746559463 25.730035021950375 28.89557539584669 23.634883835643468 30-31 22.676712869333596 26.620381788585807 29.137276180141075 21.565629161939526 32-33 20.894786168795935 27.18887189858432 28.055788487150394 23.860553445469343 34-35 21.125388447689044 30.71079761258817 27.288758447195775 20.875055492527007 36-37 23.131751590785772 28.203768559167365 25.97543530804518 22.689044542001678 38-39 21.355990726582153 26.107384205593647 28.185271050165245 24.351354017658956 40-41 20.25353919005574 25.648645982341044 29.963498248902482 24.134316578700734 42-43 22.960341340699454 25.01849750900212 29.774823657080844 22.24633749321758 44-45 19.497607655502392 27.23449908745622 29.424604153307353 23.84328910373403 46-47 22.38075272529966 24.71637152863414 29.036156464262813 23.866719281803384 48-49 21.423814926256597 24.818724411779215 28.425738667192817 25.33172199477137 50-51 19.323731070882456 25.0308291816702 30.465397326493367 25.18004242095398 52-53 18.984610072510236 25.371183347309227 32.87130666403591 22.772899916144627 54-55 18.089330636807578 25.577122280866178 30.911803877077887 25.421743205248358 56-57 20.77640211118236 25.361318009174767 28.70690080402506 25.155379075617816 58-59 18.405021457110443 26.674641148325357 30.93523405514724 23.98510333941696 60-61 20.48290830168204 29.11877867113895 26.849750900212104 23.548562126966903 62-63 17.957381739259112 31.290386227987966 26.249198441276572 24.50303359147635 64-65 18.327331919301535 32.54575050559858 25.394613525378585 23.732304049721304 66-67 18.382824446307897 32.29541755043654 24.91737779312386 24.404380210131702 68-69 18.55793419819464 32.08947861687959 25.016031174468505 24.336556010457258 70-71 18.922951709169833 31.157204163172693 24.869284269718346 25.05055985793913 72-73 18.742909288215852 29.436935825975436 25.258965126029693 26.56118975977902 74-75 19.438415626695605 29.23716272875253 25.221970108025456 26.102451536526416 76 19.387855768756474 29.295121590292506 25.750998865486114 25.566023775464902 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 1.0 18 0.5 19 1.5 20 6.0 21 9.0 22 8.5 23 15.5 24 38.5 25 54.0 26 66.0 27 97.5 28 154.0 29 191.0 30 243.0 31 348.0 32 495.0 33 589.0 34 692.5 35 866.0 36 1038.5 37 1141.0 38 1327.0 39 1978.5 40 2799.0 41 3246.5 42 3339.0 43 3318.5 44 3255.0 45 3229.0 46 3246.0 47 3181.0 48 2960.5 49 2614.0 50 2423.0 51 2160.5 52 1693.5 53 1369.5 54 1250.0 55 1123.5 56 793.5 57 530.0 58 470.0 59 374.5 60 231.5 61 141.0 62 98.0 63 74.0 64 40.0 65 20.5 66 7.0 67 3.0 68 2.5 69 1.5 70 0.5 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 40546.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.573669412519116 #Duplication Level Percentage of deduplicated Percentage of total 1 56.416023342046074 22.890050806491395 2 16.217859096711447 13.16036107137572 3 9.573886085952221 11.65343067133626 4 5.859826150385994 9.510185961623835 5 3.7140599355662265 7.5346520001973065 6 2.4132271594431947 5.874808859073645 7 1.3859339857759407 3.936269915651359 8 1.2643608291289283 4.103980663937257 9 0.8206188073673333 2.9965964583436095 >10 2.2916540027961827 12.980318650421744 >50 0.012157315664701233 0.31322448576924977 >100 0.024314631329402467 2.1703743895822027 >500 0.0 0.0 >1k 0.006078657832350617 2.875746066196419 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 1166 2.875746066196419 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 480 1.183840576135747 Illumina PCR Primer Index 9 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 167 0.41187786711389535 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 128 0.3156908203028659 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 105 0.2589651260296947 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT 71 0.17510975188674593 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 56 0.1381147338825038 No Hit TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT 48 0.1183840576135747 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC 48 0.1183840576135747 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 44 0.10851871947911015 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0369950180042421 0.0 2 0.0 0.0 0.0 0.04439402160509051 0.0 3 0.0 0.0 0.0 0.05179302520593893 0.0 4 0.0 0.0 0.0 0.06412469787401963 0.0 5 0.0 0.0 0.0 0.06412469787401963 0.0 6 0.0 0.0 0.0 0.06412469787401963 0.0 7 0.0 0.0 0.0 0.07645637054210033 0.0 8 0.0 0.0 0.0 0.08878804321018102 0.0 9 0.0 0.0 0.0 0.16277807921866522 0.0 10 0.0 0.0 0.0 0.18744142455482662 0.0 11 0.0 0.0 0.0 0.212104769890988 0.0 12 0.0 0.0 0.0 0.212104769890988 0.0 13 0.0 0.0 0.0 0.21950377349183645 0.0 14 0.0 0.0 0.0 0.229369111626301 0.0 15 0.0 0.0 0.0 0.2417007842943817 0.0 16 0.0 0.0 0.0 0.2490997878952301 0.0 17 0.0 0.0 0.0 0.2564987914960785 0.0 18 0.0 0.0 0.0 0.2762294677650076 0.0 19 0.0 0.0 0.0 0.30089281310116905 0.0 20 0.0 0.0 0.0 0.30829181670201744 0.0 21 0.0 0.0 0.0 0.3230898239037143 0.0 22 0.0 0.0 0.0 0.3304888275045627 0.0 23 0.0 0.0 0.0 0.352685838307108 0.0 24 0.0 0.0 0.0 0.35761850737434026 0.0 25 0.0 0.0 0.0 0.3896808563113501 0.0 26 0.0 0.0 0.0 0.4044788635130469 0.0 27 0.0 0.0 0.0 0.4291422088492083 0.0 28 0.0 0.0 0.0 0.458738223252602 0.0 29 0.0 0.0 0.0 0.5055985793913086 0.0 30 0.0 0.0 0.0 0.53026192472747 0.0 31 0.0 0.0 0.0 0.5894539535342573 0.0 32 0.0 0.0 0.0 0.6363143096729641 0.0 33 0.0 0.0 0.0 0.705371676614216 0.0 34 0.0 0.0 0.0 0.7448330291520742 0.0 35 0.0 0.0 0.0 0.8730824249001135 0.0 36 0.0 0.0 0.0 1.0925861983919498 0.0 37 0.0 0.0 0.0 1.3466186553544122 0.0 38 0.0 0.0 0.0 1.7634291915355398 0.0 39 0.0 0.0 0.0 2.219701080254526 0.0 40 0.0 0.0 0.0 2.7302323287130665 0.0 41 0.0 0.0 0.0 3.230898239037143 0.0 42 0.0 0.0 0.0 3.847481872441178 0.0 43 0.0 0.0 0.0 4.397474473437577 0.0 44 0.0 0.0 0.0 5.02145711044246 0.0 45 0.0 0.0 0.0 5.623242736644799 0.0 46 0.0 0.0 0.0 6.407537118334731 0.0 47 0.0 0.0 0.0 6.979726730133676 0.0 48 0.0 0.0 0.0 7.7936171262270015 0.0 49 0.0 0.0 0.0 8.402801756030188 0.0 50 0.0 0.0 0.0 8.96265969516105 0.0 51 0.0 0.0 0.0 9.549647314161692 0.0 52 0.0 0.0 0.0 10.200759631036354 0.0 53 0.0 0.0 0.0 10.782814580969763 0.0 54 0.0 0.0 0.0 11.318009174764464 0.0 55 0.0 0.0 0.0 11.831006757756622 0.0 56 0.0 0.0 0.0 12.252749963004982 0.0 57 0.0 0.0 0.0 12.780545553198836 0.0 58 0.0 0.0 0.0 13.273812459922064 0.0 59 0.0 0.0 0.0 13.784343708380606 0.0 60 0.0 0.0 0.0 14.395994672717407 0.0 61 0.0 0.0 0.0 14.901593252108716 0.0 62 0.0 0.0 0.0 15.441720514970651 0.0 63 0.0 0.0 0.0 15.9078577418241 0.0 64 0.0 0.0 0.0 16.3049376017363 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGAAT 15 0.0022093963 70.0 51 GTGTTGA 15 0.0022093963 70.0 11 GCGAAGT 15 0.0022093963 70.0 32 GGCGAAG 15 0.0022093963 70.0 31 CGGGAGG 15 0.0022093963 70.0 38 TTAAGGC 15 0.0022093963 70.0 27 CCTCACG 15 0.0022093963 70.0 33 ATTTTGT 25 2.3635043E-4 56.000004 38 TGTTGTC 20 0.006903951 52.5 57 GTCAAAA 20 0.006903951 52.5 61 TGTCAAA 20 0.006903951 52.5 60 GGCCTCA 20 0.006903951 52.5 31 ACGGGAG 20 0.006903951 52.5 37 AGTTGGA 20 0.006903951 52.5 33 TTTGGCC 20 0.006903951 52.5 28 TTGGCCT 20 0.006903951 52.5 29 CGGTTGT 20 0.006903951 52.5 55 CTCACGG 20 0.006903951 52.5 34 TTAAAAT 20 0.006903951 52.5 21 GTTGTCA 20 0.006903951 52.5 58 >>END_MODULE