FastQCFastQC Report
Wed 25 May 2016
SRR1780701_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780701_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257740
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT4080.15829906106929464No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT3970.1540311942267401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCT207.87723E-443.963481
TGCGTGT301.3031578E-436.64335333
GCTTTTC250.002332091335.2529614
CGCGCCT250.00233878435.2323839
AGTCATC506.0197635E-835.1298458
TTAAGTC250.002372476735.1298453
CAGGCTA250.002372476735.1298456
CGATTAG401.7935634E-533.05608419
TTGCGAT401.7959559E-533.0496516
GATCGTT401.8031495E-533.03035739
TTCGTCC1200.032.9406172
CATCGTT551.3719E-731.98586832
GTCATCA551.3907447E-731.936229
TGGATCC353.1790455E-431.48198520
GCGTGTA353.1899576E-431.46360634
CCTTTAG1200.031.1045467
GTTCGTC1250.029.8951681
AAGTTGT300.005689288529.38318819
TGCCCCA300.00569470729.37746617
CATTGCG454.015824E-529.37746614
Produced by FastQC (version 0.11.5)