FastQCFastQC Report
Wed 25 May 2016
SRR1780701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257740
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT119114.621323814696981No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT41111.5950182354310547TruSeq Adapter, Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC21160.820982385349577No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG11000.42678668425545124No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC8800.341429347404361No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC4110.1594630247536277No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT4000.1551951579110732No Hit
TTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATT2680.10398075580041902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACT254.435165E-543.99674
GCTACTA207.848001E-443.996744
CCGTCTT5500.039.99744
ATGATTC850.038.8206240
GCCGTCT5850.037.6040243
CGCAAAG301.2959036E-436.67815412
CAAAGGC301.2959036E-436.67815414
TTCAGCC900.036.66391844
CCCAACG301.2988677E-436.66391821
CCAACGT301.2988677E-436.66391822
AGCGGAT1050.035.6163835
ACTTAGT250.00234352935.217861
TTCTACA250.00235023535.1973572
GGCTCCC250.00235023535.19735717
GCGTGAA1150.034.432225
GGATCAC451.0336207E-634.21965418
TGAAGGT1100.033.9974528
CACGATG401.8106624E-533.01033812
TGGCGTG1200.032.99752423
GTCTATC401.8154795E-532.99752427
Produced by FastQC (version 0.11.5)