FastQCFastQC Report
Wed 25 May 2016
SRR1780700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37277
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTAC150.002208836469.99328631
CCGGAGT150.002208836469.99328631
TTTCCGG150.002208836469.99328628
GTATCCA150.002208836469.9932869
ATGGTTC150.002208836469.99328617
CGGAGTA150.002208836469.99328632
TTTTCCG150.002208836469.99328627
GTTCTTT252.362144E-455.9946320
AATTTTC200.00690222852.49496523
TTCCGGA200.00690222852.49496529
TTTTTCC200.00690222852.49496526
ATGTATC200.00690222852.4949657
TGGTTCT200.00690222852.49496518
TATAGGG200.00690222852.4949652
TGGGAAT200.00690222852.49496570
TATGTAT200.00690222852.4949656
AAATGGT200.00690222852.49496515
TATCCAA200.00690222852.49496510
GGTTCTT305.808791E-446.66219319
GTACTAT503.2388616E-642.279731
Produced by FastQC (version 0.11.5)