##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780700_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37277 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.405799822947124 34.0 27.0 34.0 12.0 34.0 2 30.646564905974195 34.0 32.0 34.0 21.0 34.0 3 30.89379510153714 34.0 32.0 34.0 21.0 34.0 4 31.15435791506827 34.0 33.0 34.0 21.0 34.0 5 30.956058695710492 34.0 32.0 34.0 21.0 34.0 6 34.01161574161011 37.0 34.0 38.0 22.0 38.0 7 33.25884593717306 37.0 34.0 38.0 21.0 38.0 8 32.320116962201894 37.0 31.0 38.0 11.0 38.0 9 33.024894707192104 37.0 34.0 38.0 21.0 38.0 10-11 33.40818735413257 37.0 34.0 38.0 21.0 38.0 12-13 33.759918984896856 37.0 34.0 38.0 21.5 38.0 14-15 33.34985379724763 37.0 34.0 38.0 21.0 38.0 16-17 33.20083429460526 37.0 34.0 38.0 21.0 38.0 18-19 33.16236553370712 37.0 33.5 38.0 16.0 38.0 20-21 33.28973629852187 37.0 34.0 38.0 21.0 38.0 22-23 33.24154304262682 37.0 34.0 38.0 21.0 38.0 24-25 33.67368618719318 37.5 34.0 38.0 21.0 38.0 26-27 33.76172975293076 38.0 34.0 38.0 21.0 38.0 28-29 33.8709525981168 38.0 34.0 38.0 21.5 38.0 30-31 33.68192182847332 37.5 34.0 38.0 21.0 38.0 32-33 33.447581618692496 37.0 34.0 38.0 21.0 38.0 34-35 33.31432250449339 37.0 34.0 38.0 21.0 38.0 36-37 33.27806690452558 37.0 34.0 38.0 21.0 38.0 38-39 33.84034391179547 37.5 34.0 38.0 21.0 38.0 40-41 33.27566596024358 37.0 34.0 38.0 21.0 38.0 42-43 33.699184483729915 37.0 34.0 38.0 21.0 38.0 44-45 32.701960994715236 37.0 31.5 38.0 15.5 38.0 46-47 33.46957909703034 37.0 34.0 38.0 21.0 38.0 48-49 33.168763580760256 37.0 34.0 38.0 21.0 38.0 50-51 33.07699117418247 37.0 33.0 38.0 16.0 38.0 52-53 33.12460766692598 37.0 33.5 38.0 21.0 38.0 54-55 32.88310486358881 37.0 32.5 38.0 20.0 38.0 56-57 32.97798910856561 37.0 33.0 38.0 16.0 38.0 58-59 32.798790138691416 37.0 32.0 38.0 16.0 38.0 60-61 32.87764573329399 37.0 32.5 38.0 15.5 38.0 62-63 32.921211470880166 37.0 32.0 38.0 19.5 38.0 64-65 32.94826568661641 37.0 32.0 38.0 19.0 38.0 66-67 32.8688065026692 37.0 32.0 38.0 19.0 38.0 68-69 32.40346594414787 37.0 31.0 38.0 15.0 38.0 70-71 32.17006465112536 37.0 31.0 38.0 11.0 38.0 72-73 32.22583630656973 37.0 31.0 38.0 11.0 38.0 74-75 31.868082195455642 37.0 30.0 38.0 11.0 38.0 76 31.00694798401159 36.0 27.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.5419150772765313 1101 2 -0.6551950486491442 1101 3 -0.0823867720656466 1101 4 -2.3653822066051085 1101 5 -1.9354180888072676 1101 6 -3.244814003677817 1101 7 -0.3403506797821336 1101 8 -1.7071332936249526 1101 9 -2.417336416553244 1101 10-11 -0.8249842357769523 1101 12-13 -0.871641447451772 1101 14-15 -0.5110926761272836 1101 16-17 -2.423702179856317 1101 18-19 -0.013365617660817009 1101 20-21 -0.7878220108943736 1101 22-23 -0.687067283821726 1101 24-25 -1.3507323957251174 1101 26-27 -0.49267656072720456 1101 28-29 0.2680972599311673 1101 30-31 -1.068726298855907 1101 32-33 -0.8342906551754368 1101 34-35 -0.9132818510071914 1101 36-37 -0.5463348758482169 1101 38-39 -0.9269071764842778 1101 40-41 -0.22645440948854656 1101 42-43 -0.6095064071877587 1101 44-45 0.2815524276105421 1101 46-47 0.22499793773255305 1101 48-49 -0.6953821187654228 1101 50-51 0.2595977768011508 1101 52-53 -0.0042400138468750015 1101 54-55 -0.7700815800152725 1101 56-57 -0.9260225429006326 1101 58-59 -0.14384874147486926 1101 60-61 0.9166071504271827 1101 62-63 -1.9721347672425296 1101 64-65 -0.0470556490089038 1101 66-67 -1.5585355153937925 1101 68-69 0.37373659736616105 1101 70-71 0.6858698427524104 1101 72-73 -0.8401213557754232 1101 74-75 -1.7986927740638734 1101 76 -2.2451931547373896 1102 1 0.2032576869511118 1102 2 -0.3320243169418262 1102 3 1.0919018458205336 1102 4 0.7067722649396089 1102 5 1.2665636185098066 1102 6 2.203864858110798 1102 7 2.5714887511121773 1102 8 2.743069958407567 1102 9 1.3913526078369998 1102 10-11 -0.3763053739883304 1102 12-13 -4.587469699484291 1102 14-15 -1.0807064972654885 1102 16-17 -2.305307870913225 1102 18-19 -0.11943777213235762 1102 20-21 0.11004384276416346 1102 22-23 -0.4450703326022136 1102 24-25 1.0763509376082112 1102 26-27 -5.494200950971106 1102 28-29 0.09317347944336518 1102 30-31 0.5245155710627856 1102 32-33 -6.036526427533158 1102 34-35 -0.49267717621044227 1102 36-37 0.732120408704624 1102 38-39 0.9604149779872699 1102 40-41 1.339602501080563 1102 42-43 0.9551531456577749 1102 44-45 -7.51138456425938 1102 46-47 0.6334074905780795 1102 48-49 1.2742571088768528 1102 50-51 -6.962836166288284 1102 52-53 -1.8134371683184192 1102 54-55 1.0139631354318865 1102 56-57 -0.5597877868030707 1102 58-59 1.8331583723462614 1102 60-61 1.463863247988158 1102 62-63 -3.7532577347222045 1102 64-65 -0.32347841323655047 1102 66-67 0.04639334639482939 1102 68-69 -7.483732914828963 1102 70-71 -1.2626819865158865 1102 72-73 0.6124955141432764 1102 74-75 -6.3442974488606225 1102 76 -0.8938212035178772 1103 1 -0.6284206619585468 1103 2 -0.638099083296968 1103 3 0.056660412798724025 1103 4 0.27024578518883047 1103 5 -0.08244339083598717 1103 6 -2.483245733789513 1103 7 -0.13713663829592804 1103 8 -0.571961194240405 1103 9 -0.04293477534056933 1103 10-11 -0.3040701714961216 1103 12-13 -0.8642901823503379 1103 14-15 -0.42669559383870137 1103 16-17 -1.2303468117231944 1103 18-19 -0.3845760120077486 1103 20-21 -0.8660075591049932 1103 22-23 -0.326057482134928 1103 24-25 0.3338470435272143 1103 26-27 -0.7564673061112899 1103 28-29 0.2662167037424297 1103 30-31 0.280259340533199 1103 32-33 -6.0758566456016965 1103 34-35 -1.255771023562474 1103 36-37 0.06467492583858103 1103 38-39 0.6125275169280755 1103 40-41 0.017562002638186414 1103 42-43 0.4633793917636879 1103 44-45 -5.6029928197110905 1103 46-47 -0.2815555156524425 1103 48-49 0.3621173113690688 1103 50-51 0.17292123246561175 1103 52-53 -0.3474130250162375 1103 54-55 0.773114225774556 1103 56-57 0.956129269271699 1103 58-59 0.5659757399475183 1103 60-61 6.623134087178073E-4 1103 62-63 -0.08040921447297933 1103 64-65 1.1866461973553513 1103 66-67 0.41798602552254494 1103 68-69 -2.2386978680999903 1103 70-71 0.009952359278507572 1103 72-73 0.6233502649220881 1103 74-75 -1.49358677907869 1103 76 -0.03137961473283113 1104 1 -1.7654923130488882 1104 2 0.5227375878200782 1104 3 -0.0015505351318481075 1104 4 0.6770103601777038 1104 5 -0.09801971482352556 1104 6 0.2127934295393743 1104 7 1.6360720844455088 1104 8 2.332355672693282 1104 9 1.3184359411703355 1104 10-11 0.3111946260116696 1104 12-13 -0.3373208899604805 1104 14-15 0.8377458836868854 1104 16-17 0.5714778433724845 1104 18-19 1.8949967516771622 1104 20-21 1.3546866999070204 1104 22-23 0.9342451435882637 1104 24-25 1.2945056995129818 1104 26-27 1.2135371442669864 1104 28-29 0.8019532413481301 1104 30-31 -0.31982966703244387 1104 32-33 0.43847357246684027 1104 34-35 0.8589597285514543 1104 36-37 0.9868823134665305 1104 38-39 0.3361590256063067 1104 40-41 1.5979358344138888 1104 42-43 1.3719686218482465 1104 44-45 -2.7462059928308094 1104 46-47 0.7159967762923714 1104 48-49 1.796429727924469 1104 50-51 1.5771935956164853 1104 52-53 0.9993604507291991 1104 54-55 0.7109869449556854 1104 56-57 0.4829205465302664 1104 58-59 1.2818190866319839 1104 60-61 1.323982295607209 1104 62-63 1.0882601224206496 1104 64-65 0.8408073010491677 1104 66-67 0.36097667972816083 1104 68-69 -3.5560543434003904 1104 70-71 -0.4137236531825508 1104 72-73 1.554013371286132 1104 74-75 0.3100180273298605 1104 76 1.3308811774345024 1105 1 -0.867344164900743 1105 2 0.5105682756507655 1105 3 0.4090314754501634 1105 4 0.5262167093840517 1105 5 0.6056839888801768 1105 6 0.8728463395922788 1105 7 1.3897757881492154 1105 8 1.1365884769260859 1105 9 1.883250755985145 1105 10-11 1.6306390704561124 1105 12-13 -0.8079558105954021 1105 14-15 0.8243860953270996 1105 16-17 1.3405254624201106 1105 18-19 1.0258168574972686 1105 20-21 -0.25338208316176036 1105 22-23 0.8367583711014888 1105 24-25 0.8946379746452493 1105 26-27 0.6377434934733373 1105 28-29 -0.9386552242603443 1105 30-31 0.22011742291464742 1105 32-33 1.0468069058001745 1105 34-35 1.3875311571228934 1105 36-37 1.1534167050009287 1105 38-39 0.84409553354282 1105 40-41 0.9321950936731511 1105 42-43 1.544852219731844 1105 44-45 2.3832913616665508 1105 46-47 1.4441713794669724 1105 48-49 1.194974701469448 1105 50-51 0.09677031519319712 1105 52-53 -0.05302050165175132 1105 54-55 0.4653520243207723 1105 56-57 0.19773536134508163 1105 58-59 0.5443852241981162 1105 60-61 0.32265954428444843 1105 62-63 0.8885246726852074 1105 64-65 0.19041047565234237 1105 66-67 0.8369026056540818 1105 68-69 1.042887455541404 1105 70-71 1.6047948653359683 1105 72-73 1.2403890326617883 1105 74-75 -0.3865428192309892 1105 76 -0.2722934257400986 1106 1 0.6309989150212871 1106 2 -0.30834010641551046 1106 3 -0.634633241898765 1106 4 0.12563191406241359 1106 5 0.7932083553519114 1106 6 0.13259292828623614 1106 7 1.895721207252528 1106 8 2.0882453970040586 1106 9 1.9219447131001601 1106 10-11 0.40242269618710225 1106 12-13 0.45140091455079556 1106 14-15 0.7269689413310019 1106 16-17 0.49227984838501726 1106 18-19 0.24311705242904225 1106 20-21 -0.12350878881478167 1106 22-23 0.33700203581884125 1106 24-25 0.9624254990117294 1106 26-27 0.30777273323941756 1106 28-29 -1.2441620468724253 1106 30-31 -0.3729624991126528 1106 32-33 0.9823332215896485 1106 34-35 0.3998118588772712 1106 36-37 0.7961554964239284 1106 38-39 0.35871541658376316 1106 40-41 0.5558305712559957 1106 42-43 1.2782342860086473 1106 44-45 0.8999092953897474 1106 46-47 1.2969491572447538 1106 48-49 0.2001891264207174 1106 50-51 0.7433590091503177 1106 52-53 -0.8372310279675403 1106 54-55 -0.5546772154453095 1106 56-57 0.8864293184600882 1106 58-59 0.04472635480240683 1106 60-61 0.9763632479881608 1106 62-63 0.6276084933479709 1106 64-65 1.0510829902722278 1106 66-67 1.1715029955176348 1106 68-69 1.9093591904341949 1106 70-71 1.5634693292735875 1106 72-73 1.105642443967831 1106 74-75 -1.2223127997378143 1106 76 -0.5869133087810354 1107 1 -1.3348138469721924 1107 2 -0.18379422844624926 1107 3 0.5109652676494427 1107 4 0.5965215274764795 1107 5 -0.10096956733089968 1107 6 1.0543442091432453 1107 7 1.6543611699912333 1107 8 1.5000758581125808 1107 9 0.6045716343856711 1107 10-11 -0.27140124419482703 1107 12-13 -0.00584174963178441 1107 14-15 -0.41606785419764947 1107 16-17 0.5566021585852994 1107 18-19 0.9953760184281109 1107 20-21 0.5984287985163732 1107 22-23 -1.1976975449915983 1107 24-25 -0.6095819532472433 1107 26-27 0.6678565711527895 1107 28-29 -1.0457922285212362 1107 30-31 0.34245436162326115 1107 32-33 1.1098600031453074 1107 34-35 0.7695370568279003 1107 36-37 -0.603425314009236 1107 38-39 0.7103688865418363 1107 40-41 0.271387161847521 1107 42-43 -0.40127200183485456 1107 44-45 -0.18164636366940812 1107 46-47 0.6898695112270516 1107 48-49 0.3815119023871816 1107 50-51 1.090215087399038 1107 52-53 0.5974219762243536 1107 54-55 1.0701946988537117 1107 56-57 1.1248084521349853 1107 58-59 0.39349207441116363 1107 60-61 -0.6130172829852967 1107 62-63 -1.3028060385570086 1107 64-65 0.7126572799787922 1107 66-67 1.2937200425600182 1107 68-69 1.8716321294188276 1107 70-71 0.4033854913602184 1107 72-73 0.4670770038187939 1107 74-75 0.31937327385324465 1107 76 -0.9438765132523912 1108 1 -1.552158979715557 1108 2 -0.1620243169418245 1108 3 0.03273517915386748 1108 4 -0.014894401727058693 1108 5 0.07531361850980645 1108 6 0.4846981914441315 1108 7 1.4394054177788433 1108 8 2.1914032917409045 1108 9 0.1851026078370026 1108 10-11 1.0495279593449993 1108 12-13 1.1598219671823742 1108 14-15 0.9201268360678441 1108 16-17 0.10385879575343893 1108 18-19 0.5928538945343078 1108 20-21 0.0025438427641617523 1108 22-23 -0.3219453326022119 1108 24-25 -1.2107323957251168 1108 26-27 0.823299049028897 1108 28-29 0.7100484794433655 1108 30-31 0.5999322377294547 1108 32-33 0.9418069058001706 1108 34-35 -0.13413550954378195 1108 36-37 0.25878707537129486 1108 38-39 1.386873311320599 1108 40-41 0.2979358344138916 1108 42-43 1.1424448123244346 1108 44-45 1.0206987690739595 1108 46-47 1.4469491572447453 1108 48-49 0.8457154422101851 1108 50-51 1.6736221670450462 1108 52-53 0.6469794983482515 1108 54-55 1.0097964687652095 1108 56-57 1.7345872131969315 1108 58-59 -0.8407999609870664 1108 60-61 1.3363632479881602 1108 62-63 1.3644505986111284 1108 64-65 1.3881882534301226 1108 66-67 0.3509766797281557 1108 68-69 1.487517085171035 1108 70-71 2.332943013484112 1108 72-73 1.553537180809947 1108 74-75 1.4071608844727166 1108 76 0.7299287964821239 1109 1 -0.21517784764008496 1109 2 -0.5065526188286178 1109 3 1.3391502734934875 1109 4 1.3396339001597362 1109 5 1.1787098449248994 1109 6 1.1728113989912998 1109 7 -1.4664436388249307 1109 8 2.1023466879673194 1109 9 1.7303856267049227 1109 10-11 2.2671694687789596 1109 12-13 1.3047276275597355 1109 14-15 1.1476740058791606 1109 16-17 0.07008521084777897 1109 18-19 1.6076652152890247 1109 20-21 1.9815061069151056 1109 22-23 2.1778659881525044 1109 24-25 0.9983242080484729 1109 26-27 1.6720726339345546 1109 28-29 1.3051428190660062 1109 30-31 1.6339888415030472 1109 32-33 2.5821842642907384 1109 34-35 1.881241848946786 1109 36-37 1.7525606602769557 1109 38-39 1.8765902924526756 1109 40-41 1.6752943249799301 1109 42-43 1.753010850060285 1109 44-45 1.1609817879418785 1109 46-47 0.7809114213957002 1109 48-49 0.31071544221018854 1109 50-51 1.5901316010073216 1109 52-53 1.5932059134425884 1109 54-55 0.8508342046142658 1109 56-57 1.5873230622535317 1109 58-59 1.2294830578808558 1109 60-61 1.7339104177994784 1109 62-63 0.8380355042715095 1109 64-65 1.3856410836188005 1109 66-67 0.9015427174640038 1109 68-69 2.148932179510659 1109 70-71 0.9416222587671328 1109 72-73 0.8342918977910756 1109 74-75 2.5011231486236625 1109 76 1.0631363436519372 1110 1 -1.8439236855979075 1110 2 -0.5428086306673165 1110 3 -0.9068726639833891 1110 4 -0.006463029178039648 1110 5 -0.3333138324705871 1110 6 0.05312956399315283 1110 7 1.583130907974919 1110 8 1.8337562329173736 1110 9 -0.9266620980453553 1110 10-11 -0.8358641975177505 1110 12-13 -0.11841332693526851 1110 14-15 0.36169546351882076 1110 16-17 0.41405487418481357 1110 18-19 0.23687350237744198 1110 20-21 -0.09461301998093319 1110 22-23 0.4198193732801343 1110 24-25 0.27671858466703725 1110 26-27 -0.9317009509711056 1110 28-29 -0.03642210879192476 1110 30-31 -0.5287932524666203 1110 32-33 1.2845520038393872 1110 34-35 0.4071390002601447 1110 36-37 -0.1453305716875306 1110 38-39 0.7150105662225599 1110 40-41 0.5136221089236983 1110 42-43 0.9193075574224778 1110 44-45 1.4353066122112068 1110 46-47 -0.35403123491211375 1110 48-49 0.5656174029944978 1110 50-51 0.8358770690058392 1110 52-53 -0.018706776161558025 1110 54-55 -0.5583407861367462 1110 56-57 -0.1219814142540514 1110 58-59 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-0.22745417757455044 2116 44-45 1.163173516548703 2116 46-47 0.12725218754778211 2116 48-49 0.007685139179884004 2116 50-51 0.322359540782422 2116 52-53 1.0277875791563318 2116 54-55 0.4034833374520801 2116 56-57 -0.2938471302374168 2116 58-59 -0.3718605670476762 2116 60-61 -0.9559599843350739 2116 62-63 0.4886930228535533 2116 64-65 0.749753909995782 2116 66-67 -0.49053847178699783 2116 68-69 0.4048403174942692 2116 70-71 -1.853117592576492 2116 72-73 0.9554563727291381 2116 74-75 -0.08940477209294428 2116 76 -1.3025964560431298 2117 1 0.8836304939686528 2117 2 0.4495546304265936 2117 3 0.8285246528380767 2117 4 -0.39015755962179455 2117 5 -0.7962653288586132 2117 6 -1.9621439138190269 2117 7 -2.72533142432642 2117 8 -1.8696493398380447 2117 9 0.07983944994226277 2117 10-11 0.04452795934499676 2117 12-13 1.051400914550797 2117 14-15 0.15854788869941672 2117 16-17 -0.09193067793076892 2117 18-19 0.564169684007986 2117 20-21 0.029122790132582566 2117 22-23 0.46858098318726604 2117 24-25 0.7413728674327729 2117 26-27 1.244614838502578 2117 28-29 0.24531163733810502 2117 30-31 0.011248027203144773 2117 32-33 1.434964800537017 2117 34-35 0.6945487009825371 2117 36-37 -0.5143708193655456 2117 38-39 0.17450489026796845 2117 40-41 -0.7020641655861084 2117 42-43 -0.10597624030713604 2117 44-45 1.2367514006529063 2117 46-47 0.2653702098763304 2117 48-49 -0.4787582420003318 2117 50-51 1.317043219676627 2117 52-53 1.2101373930850912 2117 54-55 -1.1336245838663643 2117 56-57 -0.40830752364517764 2117 58-59 0.00262109164451374 2117 60-61 1.0289948269355236 2117 62-63 -0.06186519086254805 2117 64-65 0.45634614816696484 2117 66-67 -1.6127075307981578 2117 68-69 0.21462234832893046 2117 70-71 0.6582061713788505 2117 72-73 0.9266950755467818 2117 74-75 0.24610825289376237 2117 76 -0.6711238350968252 2118 1 0.24131040803954562 2118 2 1.3771593565275602 2118 3 0.5821229342559064 2118 4 0.7416362105178393 2118 5 0.08225239402000994 2118 6 1.3012288036890283 2118 7 0.891854397370679 2118 8 -2.997576300095833 2118 9 -0.7802035146119763 2118 10-11 0.05779326546745267 2118 12-13 1.1177811508558406 2118 14-15 -0.0802813271974614 2118 16-17 0.566715938610578 2118 18-19 0.3664253231057373 2118 20-21 -1.0800071776439992 2118 22-23 0.048870993928396445 2118 24-25 0.14926760427488261 2118 26-27 0.7417684367839996 2118 28-29 -0.38546172463826167 2118 30-31 0.3920750948723182 2118 32-33 1.1207864976369066 2118 34-35 1.1749461231092795 2118 36-37 0.6691952386365969 2118 38-39 -0.04649403561818133 2118 40-41 -0.564309063545295 2118 42-43 0.9001999143652526 2118 44-45 0.6656987690739555 2118 46-47 0.2500103817345476 2118 48-49 -0.5413253741163473 2118 50-51 -0.3626023227508668 2118 52-53 -1.7622041751211377 2118 54-55 0.8154087136631745 2118 56-57 1.1975463968704005 2118 58-59 0.9382816716659903 2118 60-61 -1.4175143030322488 2118 62-63 1.324654680243782 2118 64-65 -0.21803623636579772 2118 66-67 -2.1655539325167403 2118 68-69 1.3153742280281762 2118 70-71 -0.26746514978119507 2118 72-73 -1.91503424776149 2118 74-75 0.7865486395747538 2118 76 0.30707165362498046 2119 1 -0.4959887669495977 2119 2 0.6626565341220036 2119 3 0.6340117748985463 2119 4 -0.09553269959939925 2119 5 0.29914340574384823 2119 6 0.6661875531462584 2119 7 -1.9327222417956271 2119 8 0.12161605769834694 2119 9 -0.1957484559927849 2119 10-11 0.04878327849393571 2119 12-13 0.08152409484195289 2119 14-15 -0.985405078825778 2119 16-17 -0.9963539702040052 2119 18-19 -1.3600184458912246 2119 20-21 -1.3035199870230727 2119 22-23 0.7314589227169392 2119 24-25 -0.2124345233846867 2119 26-27 0.18000117668846372 2119 28-29 0.6081335858263444 2119 30-31 0.5721662802826515 2119 32-33 -0.2696824559019504 2119 34-35 0.4070347032221804 2119 36-37 -0.5320639884584892 2119 38-39 -0.5377011567645056 2119 40-41 -0.24993650601163608 2119 42-43 -0.7801083791649219 2119 44-45 -1.222599103266468 2119 46-47 -0.4615614810531241 2119 48-49 -1.4286462599174712 2119 50-51 0.7464945074705795 2119 52-53 -0.7870630548432374 2119 54-55 -1.3800971482560627 2119 56-57 0.35809785149480433 2119 58-59 1.12781706028953 2119 60-61 0.6433845245839009 2119 62-63 -0.14831535883567426 2119 64-65 0.024039317259912707 2119 66-67 0.6982107222813525 2119 68-69 0.0714532553838012 2119 70-71 -0.63535485885631 2119 72-73 -1.858803244721976 2119 74-75 0.46077790574931043 2119 76 1.9499287964821228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 5.0 13 57.0 14 191.0 15 318.0 16 484.0 17 481.0 18 454.0 19 469.0 20 428.0 21 375.0 22 338.0 23 351.0 24 413.0 25 461.0 26 506.0 27 642.0 28 714.0 29 917.0 30 1112.0 31 1364.0 32 1725.0 33 2208.0 34 3185.0 35 4606.0 36 7352.0 37 8121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.38944608147928 22.570354951204347 11.318969479062474 31.7212294882539 2 16.92732784290581 20.334254365962927 34.91429031306168 27.824127478069588 3 18.445690372079298 25.095903640314404 28.30163371515948 28.156772272446815 4 14.926093838023446 18.32497250315208 37.04965528341873 29.699278375405747 5 13.211900099256916 34.50384955870912 33.583711135552754 18.70053920648121 6 26.453309010918257 38.055637524478904 17.259972637283045 18.231080827319797 7 25.64047536014164 31.925852402285592 21.925047616492744 20.508624621080024 8 22.214770501918075 33.56761541969579 21.58167234487754 22.635941733508595 9 22.48839767148644 17.783083402634333 23.027604152694693 36.70091477318454 10-11 23.872629235185236 25.821552163532473 26.92947393835341 23.376344662928883 12-13 23.345494540869705 24.130160688896638 25.942270032459692 26.582074737773965 14-15 21.116237894680363 25.762534538723607 24.165034739920056 28.956192826675963 16-17 21.910293210290526 27.671218177428443 25.961048367626148 24.457440244654883 18-19 21.38181720632025 27.01665906591196 26.205166724790086 25.39635700297771 20-21 20.728599404458514 27.250046945837912 26.861067145961314 25.160286503742253 22-23 22.858599136196585 26.590122595702443 26.25345387236097 24.29782439574 24-25 21.47570888215253 27.938138798723074 26.544518067441047 24.041634251683345 26-27 20.386565442498057 28.317729431016442 27.154813960350886 24.140891166134615 28-29 21.970652144754137 26.98446763419803 28.037395713174345 23.00748450787349 30-31 21.415349947688924 27.01531775625721 27.974354159401237 23.594978136652628 32-33 20.287308528046786 28.348579553075627 27.294310164444564 24.06980175443303 34-35 21.655444375888617 27.52099149609679 27.396249698205327 23.427314429809265 36-37 21.68495318829305 27.368082195455646 27.003245969364485 23.94371864688682 38-39 21.147088016739545 27.56793733401293 27.597446146417358 23.687528502830162 40-41 21.203423022238916 25.85240228559165 28.756337688118677 24.187837004050756 42-43 20.487163666604072 26.100544571719826 28.976312471497167 24.43597929017893 44-45 20.158542801191082 25.393674383668213 29.00313866459211 25.444644150548594 46-47 20.29803900528476 25.313195804383398 29.514177643050676 24.874587547281166 48-49 19.324248195938516 26.327225903372053 30.056066743568422 24.292459157121012 50-51 19.09756686428629 26.170292673766664 29.880355178796574 24.85178528315047 52-53 18.08085414598814 26.282962684765405 29.771709096762077 25.864474072484374 54-55 17.769670306086862 26.506961397108135 29.346513936207312 26.37685436059769 56-57 17.859538052954903 26.739007967379347 29.36126834240953 26.04018563725622 58-59 18.201572014915364 26.853019288032836 28.585991361965824 26.35941733508598 60-61 17.907825200525792 26.104568500684067 28.677200418488614 27.310405880301523 62-63 18.621401936851143 26.09249671379135 28.396866700646513 26.889234648711003 64-65 19.423505110389787 25.908737291091022 27.51965018644204 27.148107412077156 66-67 19.41277463315181 26.038844327601467 26.743031896343588 27.80534914290313 68-69 19.71188668616037 25.716930010462214 26.136759932397997 28.434423370979424 70-71 20.508624621080024 25.48622474984575 26.531104970893583 27.474045658180646 72-73 21.345601845642083 25.54658368430936 25.817528234568233 27.29028623548032 74-75 21.702390213804758 26.51769187434611 24.833006947984014 26.94691096386512 76 22.118196206776297 25.584140354642273 25.804115138020762 26.493548300560665 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 1.0 15 2.0 16 1.5 17 1.0 18 4.5 19 7.5 20 7.0 21 7.0 22 15.5 23 24.5 24 34.5 25 44.0 26 64.5 27 89.5 28 135.5 29 177.0 30 196.5 31 291.5 32 438.5 33 510.0 34 565.5 35 755.5 36 1013.0 37 1136.0 38 1270.0 39 1579.0 40 1895.5 41 2133.0 42 2229.0 43 2416.5 44 2658.0 45 2707.0 46 2702.0 47 2770.0 48 2781.5 49 2605.0 50 2485.0 51 2235.5 52 1854.5 53 1577.5 54 1432.0 55 1335.5 56 1060.5 57 783.0 58 684.0 59 588.0 60 414.0 61 283.5 62 231.0 63 204.0 64 152.0 65 93.0 66 59.5 67 60.0 68 47.0 69 28.5 70 18.5 71 14.0 72 14.5 73 10.0 74 8.0 75 11.0 76 9.0 77 5.5 78 4.5 79 5.0 80 3.5 81 1.0 82 1.0 83 2.0 84 2.5 85 2.5 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 3.0 92 6.0 93 3.5 94 0.5 95 0.0 96 0.0 97 1.5 98 1.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7672291225152238 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 37277.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.79185556777638 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0543818780625 51.466051452638354 2 11.707767692184111 15.639670574348793 3 5.173106273596273 10.365641011884003 4 2.5504056550726966 6.813853046114227 5 1.3615551449915655 4.547039729591973 6 0.8273756928267331 3.3157174665343243 7 0.48999919672262826 2.2909568903076964 8 0.3172945618121938 1.695415403600075 9 0.20081934291911 1.2071786892722054 >10 0.3172945618121938 2.6584757357083455 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.021460954475950318 0.0 2 0.0 0.0 0.0 0.026826193094937896 0.0 3 0.0 0.0 0.0 0.026826193094937896 0.0 4 0.0 0.0 0.0 0.03487405102341927 0.0 5 0.0 0.0 0.0 0.04023928964240685 0.0 6 0.0 0.0 0.0 0.04023928964240685 0.0 7 0.0 0.0 0.0 0.045604528261394425 0.0 8 0.0 0.0 0.0 0.061700244118357166 0.0 9 0.0 0.0 0.0 0.07511334066582612 0.0 10 0.0 0.0 0.0 0.09925691445127022 0.0 11 0.0 0.0 0.0 0.11535263030823296 0.0 12 0.0 0.0 0.0 0.12340048823671433 0.0 13 0.0 0.0 0.0 0.13144834616519568 0.0 14 0.0 0.0 0.0 0.13949620409367708 0.0 15 0.0 0.0 0.0 0.15022668133165223 0.0 16 0.0 0.0 0.0 0.1609571585696274 0.0 17 0.0 0.0 0.0 0.16363977787912118 0.0 18 0.0 0.0 0.0 0.16900501649810876 0.0 19 0.0 0.0 0.0 0.1797354937360839 0.0 20 0.0 0.0 0.0 0.20119644821203422 0.0 21 0.0 0.0 0.0 0.20119644821203422 0.0 22 0.0 0.0 0.0 0.2065616868310218 0.0 23 0.0 0.0 0.0 0.21192692545000938 0.0 24 0.0 0.0 0.0 0.22265740268798453 0.0 25 0.0 0.0 0.0 0.2360704992354535 0.0 26 0.0 0.0 0.0 0.25484883440191003 0.0 27 0.0 0.0 0.0 0.28435764680634174 0.0 28 0.0 0.0 0.0 0.32459693644874854 0.0 29 0.0 0.0 0.0 0.35142312954368643 0.0 30 0.0 0.0 0.0 0.3997102771145747 0.0 31 0.0 0.0 0.0 0.4265364702095126 0.0 32 0.0 0.0 0.0 0.45872790192343804 0.0 33 0.0 0.0 0.0 0.5445717198272393 0.0 34 0.0 0.0 0.0 0.6089545832550902 0.0 35 0.0 0.0 0.0 0.7484507873487674 0.0 36 0.0 0.0 0.0 0.8879469914424444 0.0 37 0.0 0.0 0.0 1.3252139388899322 0.0 38 0.0 0.0 0.0 1.9019770904310969 0.0 39 0.0 0.0 0.0 2.5297100088526436 0.0 40 0.0 0.0 0.0 3.254017222415967 0.0 41 0.0 0.0 0.0 4.050755157335622 0.0 42 0.0 0.0 0.0 5.126485500442632 0.0 43 0.0 0.0 0.0 6.4141427689996515 0.0 44 0.0 0.0 0.0 7.4710947769402045 0.0 45 0.0 0.0 0.0 8.479759637309868 0.0 46 0.0 0.0 0.0 9.660112133487138 0.0 47 0.0 0.0 0.0 10.961182498591626 0.0 48 0.0 0.0 0.0 12.109343563054967 0.0 49 0.0 0.0 0.0 13.06703865654425 0.0 50 0.0 0.0 0.0 14.000590176248089 0.0 51 0.0 0.0 0.0 14.840250020119646 0.0 52 0.0 0.0 0.0 15.972315368726024 0.0 53 0.0 0.0 0.0 17.005123802881133 0.0 54 0.0 0.0 0.0 18.040614856345737 0.0 55 0.0 0.0 0.0 19.04123185878692 0.0 56 0.0 0.0 0.0 19.980148617109744 0.0 57 0.0 0.0 0.0 20.88419132440915 0.0 58 0.0 0.0 0.0 21.812377605494003 0.0 59 0.0 0.0 0.0 22.504493387343402 0.0 60 0.0 0.0 0.0 23.242213697454194 0.0 61 0.0 0.0 0.0 23.96115567239853 0.0 62 0.0 0.0 0.0 24.806180754889073 0.0 63 0.0 0.0 0.0 25.731684416664432 0.0 64 0.0 0.0 0.0 26.670601174987258 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTTAC 15 0.0022088364 69.993286 31 CCGGAGT 15 0.0022088364 69.993286 31 TTTCCGG 15 0.0022088364 69.993286 28 GTATCCA 15 0.0022088364 69.993286 9 ATGGTTC 15 0.0022088364 69.993286 17 CGGAGTA 15 0.0022088364 69.993286 32 TTTTCCG 15 0.0022088364 69.993286 27 GTTCTTT 25 2.362144E-4 55.99463 20 AATTTTC 20 0.006902228 52.494965 23 TTCCGGA 20 0.006902228 52.494965 29 TTTTTCC 20 0.006902228 52.494965 26 ATGTATC 20 0.006902228 52.494965 7 TGGTTCT 20 0.006902228 52.494965 18 TATAGGG 20 0.006902228 52.494965 2 TGGGAAT 20 0.006902228 52.494965 70 TATGTAT 20 0.006902228 52.494965 6 AAATGGT 20 0.006902228 52.494965 15 TATCCAA 20 0.006902228 52.494965 10 GGTTCTT 30 5.808791E-4 46.662193 19 GTACTAT 50 3.2388616E-6 42.27973 1 >>END_MODULE