FastQCFastQC Report
Wed 25 May 2016
SRR1780700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37277
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT7482.0065992435013547No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT3510.9415993776323202RNA PCR Primer, Index 30 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1040.27899240818735416No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG590.1582745392601336No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT570.15290930064114602No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC470.12608310754620813No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC420.11267001099873916No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT410.10998739168924537No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG400.1073047723797516No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA200.006899610652.538
GGATATC200.006899610652.535
AGAGTAT200.006899610652.58
ATCGTAT200.006899610652.539
TGTATGG200.006899610652.52
ATATCGT200.006899610652.537
ATGAGCG200.006899610652.518
TTGAAAA2350.049.14893758
TCTTCTG2500.047.650
TGCTTGA2400.046.66666455
CTTGAAA2400.046.66666457
TTCTGCT2500.046.252
TCTGCTT2500.046.253
CTGCTTG2500.046.254
CTTCTGC2500.046.251
CCGTCTT2600.045.76923447
GTCTTCT2600.045.76923449
CGTCTTC2600.045.76923448
GCCGTCT2700.044.07407446
GTATGCC2550.043.9215742