##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780700_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37277 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.934356305496685 34.0 34.0 34.0 31.0 34.0 2 33.30689164900609 34.0 34.0 34.0 33.0 34.0 3 33.458513292378676 34.0 34.0 34.0 34.0 34.0 4 33.50562008745339 34.0 34.0 34.0 34.0 34.0 5 33.51128041419642 34.0 34.0 34.0 34.0 34.0 6 37.23950425195161 38.0 37.0 38.0 36.0 38.0 7 36.830646242991655 38.0 37.0 38.0 35.0 38.0 8 36.880784397886096 38.0 37.0 38.0 35.0 38.0 9 36.65316414947555 38.0 37.0 38.0 34.0 38.0 10-11 36.36255599967809 38.0 37.0 38.0 33.5 38.0 12-13 36.207379885720414 38.0 37.0 38.0 33.5 38.0 14-15 36.45395283955254 38.0 37.0 38.0 34.0 38.0 16-17 36.594066046087406 38.0 37.0 38.0 34.0 38.0 18-19 36.622206722643995 38.0 37.0 38.0 34.0 38.0 20-21 36.34907583764788 38.0 37.0 38.0 34.0 38.0 22-23 36.410065187649224 38.0 37.0 38.0 34.0 38.0 24-25 35.721691659736564 38.0 36.5 38.0 31.5 38.0 26-27 35.93675724977868 38.0 37.0 38.0 32.5 38.0 28-29 35.81696488451324 38.0 36.5 38.0 31.5 38.0 30-31 35.570244386619095 38.0 36.0 38.0 31.5 38.0 32-33 36.07011025565362 38.0 37.0 38.0 33.5 38.0 34-35 36.026973737156965 38.0 36.5 38.0 33.0 38.0 36-37 36.1682404699949 38.0 37.0 38.0 34.0 38.0 38-39 35.63954985647987 38.0 36.5 38.0 31.5 38.0 40-41 35.247538696783536 38.0 36.0 38.0 29.0 38.0 42-43 35.78449177777182 38.0 36.5 38.0 32.5 38.0 44-45 35.98425302465327 38.0 37.0 38.0 33.0 38.0 46-47 36.255519489229286 38.0 37.0 38.0 34.0 38.0 48-49 36.090068943316254 38.0 37.0 38.0 33.5 38.0 50-51 36.22208063953644 38.0 37.0 38.0 34.0 38.0 52-53 36.14696729887062 38.0 37.0 38.0 34.0 38.0 54-55 36.337782010354914 38.0 37.0 38.0 34.0 38.0 56-57 36.38557287335354 38.0 37.0 38.0 34.0 38.0 58-59 36.435724441344526 38.0 37.0 38.0 34.0 38.0 60-61 36.10742549024867 38.0 37.0 38.0 33.0 38.0 62-63 36.39186361563431 38.0 37.0 38.0 34.0 38.0 64-65 36.391380744158596 38.0 37.0 38.0 34.0 38.0 66-67 35.70826515009255 38.0 36.5 38.0 31.0 38.0 68-69 35.05125144190788 38.0 36.0 38.0 28.0 38.0 70-71 34.97798910856561 38.0 36.0 38.0 27.0 38.0 72-73 34.552767121817745 38.0 35.5 38.0 24.5 38.0 74-75 35.17034632615285 38.0 36.0 38.0 28.5 38.0 76 34.54280119108297 37.0 35.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.32763838896283914 1101 2 0.3072641562522662 1101 3 -0.33213577123959226 1101 4 0.27546842070072586 1101 5 0.21761377424100914 1101 6 -1.788597341592272 1101 7 0.3220465131931647 1101 8 0.7004458496298298 1101 9 0.46297734654326206 1101 10-11 -0.33883167763867306 1101 12-13 0.5760272688513766 1101 14-15 0.7428295467409072 1101 16-17 0.19151254783248106 1101 18-19 -0.47338939091825694 1101 20-21 0.2048353404010541 1101 22-23 0.3607225336790094 1101 24-25 -2.5532259524392273 1101 26-27 -0.5324659396431883 1101 28-29 -0.36807201581614635 1101 30-31 0.36909591569299494 1101 32-33 -0.07631507812182292 1101 34-35 0.26635445944800296 1101 36-37 0.3471297547179759 1101 38-39 0.6452750701121701 1101 40-41 1.2729107704480214 1101 42-43 0.15401832330827858 1101 44-45 0.27708547609717726 1101 46-47 0.5561349094161798 1101 48-49 -1.5014073445222706 1101 50-51 -1.5837996701318815 1101 52-53 -1.9529294666481576 1101 54-55 -1.2352192469626573 1101 56-57 -1.067173245457198 1101 58-59 -1.415056186462003 1101 60-61 -1.5344423320219889 1101 62-63 -1.0469860518323557 1101 64-65 -0.3233706166271517 1101 66-67 -1.498906245386003 1101 68-69 -0.934425668145991 1101 70-71 -0.3516021459410794 1101 72-73 -2.019979617553574 1101 74-75 -0.5140537796223583 1101 76 -0.6102182598964561 1102 1 -1.4168152182311289 1102 2 -0.014382185211147203 1102 3 0.04413455396365862 1102 4 0.23608833939991314 1102 5 -0.13223378673460218 1102 6 0.08843517873292939 1102 7 -0.015351860790573824 1102 8 0.3528848740200772 1102 9 0.8133330376001737 1102 10-11 0.578851249190599 1102 12-13 0.5612914964936522 1102 14-15 -0.44212980285258396 1102 16-17 -0.034224240785412974 1102 18-19 -0.41901947221907676 1102 20-21 0.3114156249538951 1102 22-23 0.4064542409960836 1102 24-25 -4.8276161963416655 1102 26-27 -2.010616346147245 1102 28-29 -0.03664417028769407 1102 30-31 1.5384302652864932 1102 32-33 0.4461696779757389 1102 34-35 0.5055566952203563 1102 36-37 -3.007799107070646 1102 38-39 -0.32271273476587936 1102 40-41 0.48886605500086944 1102 42-43 0.6818384452595012 1102 44-45 0.5983507199996154 1102 46-47 0.746810722424307 1102 48-49 -3.4472914908637335 1102 50-51 -0.42716857257090624 1102 52-53 0.18337642766078233 1102 54-55 0.5753752652324664 1102 56-57 0.5766275675509362 1102 58-59 0.1770423907737637 1102 60-61 -5.386449445843127 1102 62-63 -2.787204547767324 1102 64-65 -0.023827933700324877 1102 66-67 0.5513986326627744 1102 68-69 -0.5963921315606271 1102 70-71 -0.6784568207378285 1102 72-73 -6.257911528122676 1102 74-75 -2.2517316251508177 1102 76 0.11386710595720473 1103 1 0.7464441275632652 1103 2 0.2712486559103482 1103 3 -0.559740056628236 1103 4 0.29381809017872484 1103 5 0.3400208861625913 1103 6 0.44162832515037564 1103 7 0.9457073292405767 1103 8 -0.009264658690206318 1103 9 0.37476216532603246 1103 10-11 0.3113278847046175 1103 12-13 -0.3968471327898442 1103 14-15 -0.7871453791765788 1103 16-17 0.24240153803079068 1103 18-19 0.6874836430457236 1103 20-21 -0.3687342971644796 1103 22-23 0.6763140540801942 1103 24-25 -3.1313545140986747 1103 26-27 -0.3168858165522366 1103 28-29 -0.5149833447425181 1103 30-31 0.5509400004889784 1103 32-33 1.2357432761065823 1103 34-35 -0.2616259371783869 1103 36-37 -0.7158403843292049 1103 38-39 -0.6305398375696214 1103 40-41 -0.32869625029508853 1103 42-43 -0.7730388911891026 1103 44-45 0.7430645050463482 1103 46-47 1.015161579122129 1103 48-49 -1.9510882198356967 1103 50-51 -0.11656693705688781 1103 52-53 0.7751307111498846 1103 54-55 0.31880400355022687 1103 56-57 0.45727398499641225 1103 58-59 0.10466116024417715 1103 60-61 -4.199874290079883 1103 62-63 -1.101408208203459 1103 64-65 0.09445477658004364 1103 66-67 -0.018110713131619605 1103 68-69 0.708952494607594 1103 70-71 0.8133169331562584 1103 72-73 -4.988295095100874 1103 74-75 -2.420880768452996 1103 76 -2.1437263519867216 1104 1 0.5989585912926785 1104 2 0.482641624312663 1104 3 0.5337178872969943 1104 4 -0.013614041552472145 1104 5 -0.16705521530602851 1104 6 -0.23180291650516693 1104 7 0.5989338534951401 1104 8 0.7885991597343605 1104 9 0.9654163709335108 1104 10-11 -0.1437677984284491 1104 12-13 0.4845057822079326 1104 14-15 0.6602511495283636 1104 16-17 -0.01353971697588463 1104 18-19 0.7827662420666428 1104 20-21 -0.43099508933181596 1104 22-23 0.35050186004370687 1104 24-25 0.4533361846107198 1104 26-27 1.3530741300432254 1104 28-29 1.5879094011408839 1104 30-31 0.5397695510007736 1104 32-33 0.3183422970233565 1104 34-35 0.5875507428394044 1104 36-37 1.4585699405484078 1104 38-39 1.4695491699960286 1104 40-41 1.6466041502389643 1104 42-43 0.18049915954522078 1104 44-45 0.34195191047580664 1104 46-47 0.8255309605195436 1104 48-49 0.5339585091362693 1104 50-51 0.6807183321909989 1104 52-53 0.8888823800417427 1104 54-55 0.27314312237532334 1104 56-57 0.21650851993188525 1104 58-59 -0.2287611806548 1104 60-61 0.8994136493949654 1104 62-63 0.5010394998517285 1104 64-65 0.3449220662996737 1104 66-67 0.49604148980563423 1104 68-69 2.0001852493917553 1104 70-71 1.628537226881221 1104 72-73 1.8461063290201807 1104 74-75 0.6513933748491851 1104 76 0.40731948690958575 1105 1 0.933879226213314 1105 2 0.6638585555295933 1105 3 0.12631047988958954 1105 4 -0.09377277171120113 1105 5 -0.2607060089568236 1105 6 0.44491666021441034 1105 7 0.5864999910612738 1105 8 -0.3785966074614038 1105 9 0.8561571116742499 1105 10-11 0.791814212153561 1105 12-13 -0.34380109609894305 1105 14-15 0.35416649344370654 1105 16-17 0.563576685140518 1105 18-19 0.8749620092624113 1105 20-21 0.2934758101390855 1105 22-23 0.29534312988496936 1105 24-25 0.5890504703250059 1105 26-27 0.5333651353342361 1105 28-29 1.0738882371197178 1105 30-31 1.3781293393605623 1105 32-33 1.0891094927905485 1105 34-35 -0.1767349714463009 1105 36-37 0.7340990410775063 1105 38-39 0.5407131911600445 1105 40-41 0.560625314260129 1105 42-43 0.5273245563706155 1105 44-45 1.0699942385181345 1105 46-47 0.9800283150169022 1105 48-49 0.20432887950663314 1105 50-51 0.8454008718735366 1105 52-53 0.1272421684015299 1105 54-55 0.5863706356028331 1105 56-57 0.5743127527361125 1105 58-59 0.7482229463293208 1105 60-61 1.1430644430457662 1105 62-63 0.1311982300104617 1105 64-65 0.9125146588922703 1105 66-67 1.0027875215516602 1105 68-69 0.47941805362456336 1105 70-71 1.065177438521431 1105 72-73 1.2565560644699119 1105 74-75 0.46991189336770844 1105 76 0.7504411800312809 1106 1 -0.12635469191533844 1106 2 0.2731178147888542 1106 3 0.3863494662443614 1106 4 0.2866365850139445 1106 5 -0.29637852357670624 1106 6 0.2915053541715267 1106 7 -1.2171062467554918 1106 8 0.018674347704290994 1106 9 -1.0766888922243822 1106 10-11 0.567009143927443 1106 12-13 1.4058090403532972 1106 14-15 0.20085265328776813 1106 16-17 0.5503700574602064 1106 18-19 0.6456735102370672 1106 20-21 0.10258887056793498 1106 22-23 0.5051384515223987 1106 24-25 -0.2749846173942956 1106 26-27 0.8207433029755578 1106 28-29 0.672621180589509 1106 30-31 1.0873885986198246 1106 32-33 0.27297888850205965 1106 34-35 1.032914151360714 1106 36-37 0.9407754543328721 1106 38-39 0.0068925283920151514 1106 40-41 1.0969800900885858 1106 42-43 0.918845024206874 1106 44-45 1.2672651936838264 1106 46-47 0.7681374768102742 1106 48-49 1.2655374565046884 1106 50-51 0.26016695374488563 1106 52-53 0.7189575680116675 1106 54-55 0.22652658102194323 1106 56-57 0.13956616404215794 1106 58-59 0.851439320598331 1106 60-61 0.2901404664375775 1106 62-63 0.8200871188993446 1106 64-65 -0.5077095126476934 1106 66-67 0.6599512642417196 1106 68-69 1.192917078965685 1106 70-71 0.40898835470076733 1106 72-73 0.4240511911755718 1106 74-75 -0.1731680286595889 1106 76 0.06847236911509924 1107 1 0.7770454012378991 1107 2 -0.07909457459167868 1107 3 0.1000895687129244 1107 4 0.11542093527011588 1107 5 -0.09600591062840635 1107 6 0.2569455032167056 1107 7 0.49644754923892265 1107 8 -0.10065494898876892 1107 9 -0.6345836290664906 1107 10-11 0.3277450545003333 1107 12-13 -0.03955658610222201 1107 14-15 0.1825014655839965 1107 16-17 0.8319722931378948 1107 18-19 0.837802061704231 1107 20-21 0.5526121588772099 1107 22-23 0.30579052418192276 1107 24-25 0.5794634496760338 1107 26-27 0.16065946506219575 1107 28-29 0.892882009653313 1107 30-31 0.6528753242835421 1107 32-33 0.8727460054093683 1107 34-35 -0.37658747882093735 1107 36-37 1.3023963206579694 1107 38-39 0.555052751959785 1107 40-41 0.23573183671178555 1107 42-43 0.9715231797727739 1107 44-45 0.5912987288491749 1107 46-47 1.0128044539877266 1107 48-49 0.8295337303752035 1107 50-51 0.753832533623779 1107 52-53 0.6748495102566565 1107 54-55 0.28679672540945944 1107 56-57 1.154983024778069 1107 58-59 0.901369455670519 1107 60-61 1.1295442857203 1107 62-63 0.9404411012002285 1107 64-65 1.0643910928483464 1107 66-67 0.37606677425569757 1107 68-69 1.3661355233066317 1107 70-71 0.12929576038311552 1107 72-73 0.7874885456236385 1107 74-75 -0.8037688670387269 1107 76 0.6653051590545473 1108 1 -0.9505652182311266 1108 2 0.14311781478885166 1108 3 -0.1562821127030034 1108 4 0.21400500606657857 1108 5 0.13151621326539953 1108 6 0.03676851206626708 1108 7 0.2713148058760879 1108 8 0.09288487402007917 1108 9 0.28541637093351113 1108 10-11 0.5988512491905951 1108 12-13 1.0771248298269853 1108 14-15 0.6995368638140746 1108 16-17 -0.10068257411874271 1108 18-19 0.4751471944476009 1108 20-21 0.9049572916205619 1108 22-23 0.05145424099607965 1108 24-25 0.8973838036583288 1108 26-27 -0.6247830128139142 1108 28-29 -0.3568525036210204 1108 30-31 0.42738859861982803 1108 32-33 0.9392946779757381 1108 34-35 1.0865983618870274 1108 36-37 1.4426175595960231 1108 38-39 1.1797872652341184 1108 40-41 1.2130327216675383 1108 42-43 0.026213445259500645 1108 44-45 1.1564757199996194 1108 46-47 1.3107690557576461 1108 48-49 1.1839585091362679 1108 50-51 1.0359564274290918 1108 52-53 -0.366831905672548 1108 54-55 -0.1589997347675336 1108 56-57 0.7074609008842643 1108 58-59 0.2843340574404323 1108 60-61 1.0475088874902099 1108 62-63 1.0600871188993466 1108 64-65 0.6299220662996774 1108 66-67 1.0388986326627716 1108 68-69 1.1042328684393752 1108 70-71 -0.3107484874044957 1108 72-73 -0.3443698614560091 1108 74-75 -1.2452732918174831 1108 76 0.746367105957205 1109 1 -1.180376538985847 1109 2 0.45613668271337815 1109 3 0.32617071748567383 1109 4 0.32079745889676303 1109 5 0.3766105528880388 1109 6 0.21186285168890606 1109 7 -0.32038330733145415 1109 8 0.5557150626993206 1109 9 1.0163597671599263 1109 10-11 0.9349833246622978 1109 12-13 0.5491059619024554 1109 14-15 0.8330274298518177 1109 16-17 0.8962985579567331 1109 18-19 0.9870339869004283 1109 20-21 1.192410121809246 1109 22-23 0.9465485806187246 1109 24-25 0.3374781432809755 1109 26-27 0.5809717041672116 1109 28-29 -0.03949401305498412 1109 30-31 0.731728221261335 1109 32-33 0.2719361874097004 1109 34-35 0.18961722981155305 1109 36-37 0.7974288803507434 1109 38-39 1.4449759444794026 1109 40-41 0.581240268837341 1109 42-43 1.1379115584670458 1109 44-45 1.1363813803769744 1109 46-47 1.0368067916067005 1109 48-49 0.9801849242306062 1109 50-51 0.7570885029007925 1109 52-53 -0.45626586793670043 1109 54-55 0.08864177466642786 1109 56-57 0.22953637258238047 1109 58-59 -0.018024433125603423 1109 60-61 0.9884522837166188 1109 62-63 0.03895504342764866 1109 64-65 0.5392616889411883 1109 66-67 0.4558797647382491 1109 68-69 0.11262909485446926 1109 70-71 0.7076477390105964 1109 72-73 1.0735546668458724 1109 74-75 0.43095312327685775 1109 76 1.0207067285987108 1110 1 -0.7458593358781869 1110 2 -0.9249214008974249 1110 3 0.06312965200287834 1110 4 0.3065540256744228 1110 5 0.4026926838536298 1110 6 -0.14440795852196686 1110 7 0.7983736294055035 1110 8 0.3675907563730192 1110 9 1.0026712728942968 1110 10-11 -0.3010507115937102 1110 12-13 0.7487914964936522 1110 14-15 0.6510074520493774 1110 16-17 0.16461154352831642 1110 18-19 0.06259817483974928 1110 20-21 1.2038788602480182 1110 22-23 -0.3361928178274525 1110 24-25 -1.2246750198710714 1110 26-27 1.1523738499311804 1110 28-29 0.1566769081436874 1110 30-31 0.18150624567864782 1110 32-33 0.8992946779757389 1110 34-35 1.190912087377221 1110 36-37 1.490362657635238 1110 38-39 0.6883166769988307 1110 40-41 -0.6349084548030604 1110 42-43 0.7789585432987138 1110 44-45 0.9828482690192288 1110 46-47 0.5786121930125461 1110 48-49 1.0594487052146917 1110 50-51 -0.22404357257090624 1110 52-53 0.4110112315823571 1110 54-55 -0.02909777398322433 1110 56-57 0.7935393322568132 1110 58-59 -0.6994894719713329 1110 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2116 40-41 -0.8425733389385286 2116 42-43 -0.8741905951445403 2116 44-45 -0.1757970072731183 2116 46-47 -0.9863016513130631 2116 48-49 0.3087059838837405 2116 50-51 -0.8503061988335361 2116 52-53 -0.015013723854359284 2116 54-55 -0.49107044183824655 2116 56-57 -0.15001384659048256 2116 58-59 -2.158342710236333 2116 60-61 -1.7837032337219156 2116 62-63 0.42412752293974876 2116 64-65 -1.7368961155185119 2116 66-67 -0.3015054077412671 2116 68-69 -1.4848075356010284 2116 70-71 0.7920292903732857 2116 72-73 0.9152260981399465 2116 74-75 0.10290852636433812 2116 76 -1.7857541061640063 2117 1 0.8104874133478219 2117 2 0.19943360426253776 2117 3 0.5126652557180478 2117 4 0.3182155323823679 2117 5 0.38783200273908136 2117 6 0.2178211436452102 2117 7 0.509209542718196 2117 8 0.03972697928323754 2117 9 -1.2661625764349083 2117 10-11 -0.3540434876515022 2117 12-13 -1.852085696488814 2117 14-15 -0.19388418881749914 2117 16-17 -0.6759457320134814 2117 18-19 -0.9174843844997724 2117 20-21 0.028904660041618513 2117 22-23 0.17882266204871655 2117 24-25 -1.96972145949956 2117 26-27 -1.9476777496560231 2117 28-29 -0.922115661515754 2117 30-31 -0.22840087506438778 2117 32-33 -1.43754742728742 2117 34-35 0.6802825724133399 2117 36-37 -0.8486982298776624 2117 38-39 -0.1457390505553562 2117 40-41 -1.0188093835956238 2117 42-43 -0.3285233968457675 2117 44-45 -0.7169453326319655 2117 46-47 -0.6002835758213081 2117 48-49 -0.3291993856005746 2117 50-51 -0.2082540988866981 2117 52-53 0.2979049364327153 2117 54-55 -0.6155786821359541 2117 56-57 0.18167142720005103 2117 58-59 -1.7801396267700937 2117 60-61 -0.2519647967203227 2117 62-63 -0.006228670574337514 2117 64-65 -0.3024463547529592 2117 66-67 -1.0874171568109077 2117 68-69 -0.07024081577115027 2117 70-71 -0.25943269793080503 2117 72-73 1.2029985595966153 2117 74-75 1.3163056555509343 2117 76 0.700051316483524 2118 1 0.6629041695239763 2118 2 0.5506688351970155 2118 3 -0.45607803107035494 2118 4 0.21971929178086214 2118 5 0.11457743775519447 2118 6 0.16411545084177703 2118 7 0.6309066426107819 2118 8 0.6232930372853858 2118 9 -2.0121346494746533 2118 10-11 -1.4849242610134823 2118 12-13 -1.3566506803771006 2118 14-15 0.4847409454467311 2118 16-17 -2.866600941465684 2118 18-19 -0.7944446422871039 2118 20-21 -0.23269576960392158 2118 22-23 0.6232909756899616 2118 24-25 1.1060572730460905 2118 26-27 -2.576517706691469 2118 28-29 -0.12093413627408012 2118 30-31 -0.44934609525773084 2118 32-33 -0.08539919957528497 2118 34-35 -0.129115923827257 2118 36-37 -3.2805457057101037 2118 38-39 0.06376685707085272 2118 40-41 -0.975844829352873 2118 42-43 0.8294787513819557 2118 44-45 -2.229136524898344 2118 46-47 0.057503749635195334 2118 48-49 0.11967279485055116 2118 50-51 -0.3924109195096861 2118 52-53 -1.1713217015909123 2118 54-55 -1.0901221837471269 2118 56-57 0.495420084557729 2118 58-59 -0.4212781874575242 2118 60-61 -0.46861356148938427 2118 62-63 -1.3982802280394324 2118 64-65 0.3990036989527326 2118 66-67 0.3358374081729778 2118 68-69 -0.8613793764585864 2118 70-71 0.20812906361591388 2118 72-73 1.2770587099725574 2118 74-75 0.21840017757027397 2118 76 0.17126506514087936 2119 1 0.31922201581142673 2119 2 -0.7204992064877445 2119 3 0.001802993679973497 2119 4 0.008473091172959357 2119 5 0.15300557496752276 2119 6 -0.4479123389975612 2119 7 -0.944429874974972 2119 8 0.3050125335945424 2119 9 -0.1239453311941503 2119 10-11 -0.6897657720859982 2119 12-13 -0.013619851024081697 2119 14-15 0.13411133189918445 2119 16-17 -0.2568527868846999 2119 18-19 -0.07017195448857194 2119 20-21 -1.2489788785921974 2119 22-23 -0.298332993046472 2119 24-25 0.6085540164242929 2119 26-27 0.4517063488882087 2119 28-29 -0.1987673972380435 2119 30-31 0.8076013645772662 2119 32-33 0.4950393588268014 2119 34-35 0.9917047448657499 2119 36-37 0.39889415534070594 2119 38-39 -1.6139361390211988 2119 40-41 -0.8777119591835287 2119 42-43 0.5917453601531122 2119 44-45 0.06743316680812939 2119 46-47 0.17821586426828162 2119 48-49 -0.03838191639565025 2119 50-51 0.6802117465780313 2119 52-53 0.12763617943383565 2119 54-55 0.3064257971473552 2119 56-57 -1.0780710140093532 2119 58-59 -0.4278999851127594 2119 60-61 0.0928280364263756 2119 62-63 -0.11289160450490243 2119 64-65 -0.2572055932747972 2119 66-67 0.45166459010957993 2119 68-69 1.4245520173755395 2119 70-71 0.5887195977018891 2119 72-73 0.9077577981184604 2119 74-75 0.013450112437837447 2119 76 0.5859415740423088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 6.0 23 3.0 24 16.0 25 35.0 26 66.0 27 109.0 28 224.0 29 373.0 30 607.0 31 865.0 32 1427.0 33 2235.0 34 3418.0 35 5023.0 36 8521.0 37 14347.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.13963033505915 23.937012098613085 11.967164739651796 28.956192826675963 2 17.04536309252354 19.53751643104327 38.903345226278944 24.51377525015425 3 17.276068353140005 25.482200820881506 30.563081793062747 26.67864903291574 4 13.289696059232234 20.125010059822408 37.264264828178234 29.321029052767123 5 12.388335971242322 35.737854441076266 36.87260240899214 15.001207178689272 6 26.415752340585346 38.85237545939856 17.756257209539395 16.9756149904767 7 25.97312015451887 29.94339673256968 23.057112965099122 21.026370147812322 8 22.57424148939024 35.99538589478767 21.039783244359793 20.390589371462294 9 22.25500979156048 16.21643372588996 22.244279314322505 39.28427716822706 10-11 23.73983957936529 26.209190653754327 27.924725702175607 22.12624406470478 12-13 23.574858491831424 23.28379429675135 26.971054537650563 26.170292673766664 14-15 21.467661024224054 24.858491831424203 23.14429809265767 30.529549051694072 16-17 22.631917804544358 28.59135660058481 24.861174450733696 23.915551144137133 18-19 22.24293800466776 27.298334093408805 26.16895136411192 24.28977653781152 20-21 21.094776940204415 26.980443705233792 25.83496526007994 26.08981409448185 22-23 22.221477050191808 28.127263460042386 26.305764948896105 23.345494540869705 24-25 22.627893875580117 28.695978753655073 25.89264157523406 22.783485795530755 26-27 21.67958794967406 29.621482415430428 26.337956380610027 22.360973254285486 28-29 21.536067816616146 28.191646323470238 27.423075891300265 22.849209968613355 30-31 20.594468438983824 26.1340773130885 29.671110872656058 23.600343375271613 32-33 22.767390079673795 26.48281782332269 26.839606191485366 23.910185905518148 34-35 22.749953054162084 26.732301419105614 27.276873138932856 23.240872387799445 36-37 23.309279180191538 27.54513506988223 26.42648281782332 22.719102932102906 38-39 21.102824798132897 26.82216916597366 28.325777288944927 23.74922874694852 40-41 20.6910427341256 25.6820559594388 29.531614668562383 24.09528663787322 42-43 22.49778683906967 25.29575877887169 29.538321216836117 22.668133165222525 44-45 19.795047884754673 26.127370764814767 29.689889207822517 24.387692142608042 46-47 21.937119403385466 23.898114118625426 29.321029052767123 24.84373742522199 48-49 20.78895833892212 23.90482066689916 29.966198996700378 25.340021997478342 50-51 18.939292325026155 24.588888590820076 31.24849102663841 25.22332805751536 52-53 18.475199184483728 25.007377203101104 33.10218096949862 23.415242642916546 54-55 17.30021192692545 25.306489256109664 31.345065321780186 26.048233495184697 56-57 19.332296053866997 25.35209378437106 29.42967513480162 25.885935026960322 58-59 17.36191217104381 26.541835448131557 31.043270649462134 25.0529817313625 60-61 19.042573168441667 27.9475279663063 28.045443571102822 24.964455294149207 62-63 16.667113769884917 29.75427207125037 27.329184215467983 26.249429943396734 64-65 17.328379429675135 30.57381227030072 26.2628430399442 25.83496526007994 66-67 17.587252193041287 29.89779220430829 25.173699600289723 27.3412560023607 68-69 17.754915899884647 29.91120530085575 24.633151809426725 27.700726989832873 70-71 17.919896987418515 29.58392574509751 24.547307991522924 27.948869275961048 72-73 18.161332725272956 28.568554336454117 24.52182310808273 28.7482898301902 74-75 18.805161359551466 28.964240684604448 24.315261421251712 27.915336534592377 76 18.9634358988116 28.623548032298736 23.872629235185236 28.540386833704428 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 2.0 17 4.0 18 3.5 19 2.0 20 5.5 21 10.0 22 14.5 23 18.5 24 30.0 25 42.0 26 73.0 27 115.0 28 147.0 29 168.0 30 197.0 31 285.5 32 443.0 33 541.0 34 607.0 35 747.5 36 960.0 37 1098.0 38 1219.5 39 1547.0 40 1873.5 41 2295.5 42 2597.0 43 2797.5 44 3163.0 45 3249.5 46 3171.0 47 3045.0 48 2807.0 49 2542.5 50 2390.0 51 2184.0 52 1759.0 53 1352.0 54 1164.0 55 1107.0 56 855.0 57 626.0 58 592.0 59 456.5 60 266.5 61 174.0 62 136.0 63 119.0 64 84.5 65 56.0 66 29.0 67 13.0 68 8.5 69 3.0 70 1.5 71 1.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 37277.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.68809185288515 #Duplication Level Percentage of deduplicated Percentage of total 1 62.91611537443769 31.890978351262174 2 17.226779571315163 17.46385170480457 3 8.45197142101085 12.852429111784748 4 4.472082561524212 9.06725326608901 5 2.55093940195819 6.465112535880032 6 1.5295051600952634 4.651661882662231 7 0.804445620534533 2.854306945301392 8 0.5980418100026462 2.425087855782386 9 0.460439269648055 2.1004909193336374 >10 0.9632177824821382 6.690452557877512 >50 0.010584810796507011 0.31118383990127957 >100 0.010584810796507011 1.2205917858196742 >500 0.005292405398253506 2.0065992435013547 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 748 2.0065992435013547 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 351 0.9415993776323202 RNA PCR Primer, Index 30 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 104 0.27899240818735416 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 59 0.1582745392601336 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT 57 0.15290930064114602 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 47 0.12608310754620813 No Hit CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC 42 0.11267001099873916 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT 41 0.10998739168924537 No Hit CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG 40 0.1073047723797516 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.026826193094937896 0.0 2 0.0 0.0 0.0 0.03487405102341927 0.0 3 0.0 0.0 0.0 0.03487405102341927 0.0 4 0.0 0.0 0.0 0.042921908951900636 0.0 5 0.0 0.0 0.0 0.045604528261394425 0.0 6 0.0 0.0 0.0 0.045604528261394425 0.0 7 0.0 0.0 0.0 0.050969766880382 0.0 8 0.0 0.0 0.0 0.061700244118357166 0.0 9 0.0 0.0 0.0 0.0804785792848137 0.0 10 0.0 0.0 0.0 0.10730477237975158 0.0 11 0.0 0.0 0.0 0.1260831075462081 0.0 12 0.0 0.0 0.0 0.1368135847841833 0.0 13 0.0 0.0 0.0 0.14754406202215845 0.0 14 0.0 0.0 0.0 0.15022668133165223 0.0 15 0.0 0.0 0.0 0.1609571585696274 0.0 16 0.0 0.0 0.0 0.17437025511709633 0.0 17 0.0 0.0 0.0 0.17705287442659012 0.0 18 0.0 0.0 0.0 0.1824181130455777 0.0 19 0.0 0.0 0.0 0.19046597097405907 0.0 20 0.0 0.0 0.0 0.21192692545000938 0.0 21 0.0 0.0 0.0 0.21192692545000938 0.0 22 0.0 0.0 0.0 0.21729216406899696 0.0 23 0.0 0.0 0.0 0.23070526061646593 0.0 24 0.0 0.0 0.0 0.2360704992354535 0.0 25 0.0 0.0 0.0 0.24948359578292245 0.0 26 0.0 0.0 0.0 0.2736271695683666 0.0 27 0.0 0.0 0.0 0.29777074335381065 0.0 28 0.0 0.0 0.0 0.3353274136867237 0.0 29 0.0 0.0 0.0 0.3648362260911554 0.0 30 0.0 0.0 0.0 0.4184886122810312 0.0 31 0.0 0.0 0.0 0.44531480537596907 0.0 32 0.0 0.0 0.0 0.48555409501837593 0.0 33 0.0 0.0 0.0 0.5821283901601524 0.0 34 0.0 0.0 0.0 0.6465112535880033 0.0 35 0.0 0.0 0.0 0.8021031735386431 0.0 36 0.0 0.0 0.0 0.944281996941814 0.0 37 0.0 0.0 0.0 1.403009898865252 0.0 38 0.0 0.0 0.0 2.001234004882367 0.0 39 0.0 0.0 0.0 2.663840974327333 0.0 40 0.0 0.0 0.0 3.5035008181988894 0.0 41 0.0 0.0 0.0 4.38876519033184 0.0 42 0.0 0.0 0.0 5.518147919628725 0.0 43 0.0 0.0 0.0 6.888966386780052 0.0 44 0.0 0.0 0.0 8.080049360195295 0.0 45 0.0 0.0 0.0 9.201384231563699 0.0 46 0.0 0.0 0.0 10.454167449097298 0.0 47 0.0 0.0 0.0 11.841081632105588 0.0 48 0.0 0.0 0.0 13.085816991710706 0.0 49 0.0 0.0 0.0 14.132038522413284 0.0 50 0.0 0.0 0.0 15.113877189688012 0.0 51 0.0 0.0 0.0 16.036698232153874 0.0 52 0.0 0.0 0.0 17.289481449687475 0.0 53 0.0 0.0 0.0 18.389355366579927 0.0 54 0.0 0.0 0.0 19.489229283472383 0.0 55 0.0 0.0 0.0 20.58373796174585 0.0 56 0.0 0.0 0.0 21.603133299353487 0.0 57 0.0 0.0 0.0 22.598385063175684 0.0 58 0.0 0.0 0.0 23.596319446307376 0.0 59 0.0 0.0 0.0 24.33672237572766 0.0 60 0.0 0.0 0.0 25.117364594790352 0.0 61 0.0 0.0 0.0 25.911419910400515 0.0 62 0.0 0.0 0.0 26.81277999839043 0.0 63 0.0 0.0 0.0 27.808031762212625 0.0 64 0.0 0.0 0.0 28.84352281567723 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGTA 20 0.0068996106 52.5 38 GGATATC 20 0.0068996106 52.5 35 AGAGTAT 20 0.0068996106 52.5 8 ATCGTAT 20 0.0068996106 52.5 39 TGTATGG 20 0.0068996106 52.5 2 ATATCGT 20 0.0068996106 52.5 37 ATGAGCG 20 0.0068996106 52.5 18 TTGAAAA 235 0.0 49.148937 58 TCTTCTG 250 0.0 47.6 50 TGCTTGA 240 0.0 46.666664 55 CTTGAAA 240 0.0 46.666664 57 TTCTGCT 250 0.0 46.2 52 TCTGCTT 250 0.0 46.2 53 CTGCTTG 250 0.0 46.2 54 CTTCTGC 250 0.0 46.2 51 CCGTCTT 260 0.0 45.769234 47 GTCTTCT 260 0.0 45.769234 49 CGTCTTC 260 0.0 45.769234 48 GCCGTCT 270 0.0 44.074074 46 GTATGCC 255 0.0 43.92157 42 >>END_MODULE