FastQCFastQC Report
Wed 25 May 2016
SRR1780699_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780699_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577883
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA357.184415E-637.78993216
CACGACT301.2837349E-436.74659718
ACGACTC250.002325791635.27979719
TCACGAC250.002329760535.2675417
ACTCGCT250.002340702835.23387540
CTAAGTA250.002360701835.17283228
GTACCAA250.002362709335.1667432
GTACATG1150.030.5983281
TAAGACT1100.029.9483099
CTTCATG300.00567452429.40494220
AATCACG300.005686586329.3921715
TGATAAC300.00571561829.36156535
TAAAGCA300.00571561829.36156539
ATCTTGA300.00574476729.33102211
AAAGCGA1150.028.68841725
TCCGTCT406.92818E-427.56713520
GCGAACT1200.027.47877928
AGACTTC1250.026.39791911
TACATGC1250.026.3545132
GTACAAG858.245479E-925.8735851
Produced by FastQC (version 0.11.5)