FastQCFastQC Report
Wed 25 May 2016
SRR1780699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577883
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT156332.7052188764853784No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT57310.9917232380949085TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC21810.37741203669254847No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG13540.23430348357712547No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC8390.14518509802157184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGT207.8587834E-443.99368323
CGTGATT207.8587834E-443.99368327
AAGCGCT207.8587834E-443.99368330
GTAGACG207.8587834E-443.99368322
GGACGTT207.8587834E-443.99368321
TTAAGCG301.3013932E-436.661428
CCGTCTT7700.035.4296244
GAGATAT250.00235243835.1979935
GACGTTT250.00235343735.19494622
AGAGTCG250.00235343735.19494622
CGGATTA250.00235343735.19494627
ACGGATT250.00235343735.19494626
AGTCGTG250.00235343735.19494624
GCCGTCT8050.033.88920243
GCAAGCG300.005736879529.33927715
AGCTTCG300.00574659429.3291223
GCTTCGT300.00574659429.3291224
TTAGTCG300.00574659429.3291219
CGTTTTA300.00574659429.3291224
TCGCGCG300.00574659429.3291227
Produced by FastQC (version 0.11.5)