FastQCFastQC Report
Wed 25 May 2016
SRR1780698_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780698_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84460
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACTA150.00221864769.9928969
CGGTTTA150.00221864769.9928949
CTAAATT150.00221864769.9928965
CCGGTGA150.00221864769.9928950
TGACTGC252.379523E-455.99431642
GCCATAG200.00673972952.8703081
CGGCGGC200.00693264652.49467522
ACTGCGC200.00693264652.49467544
GAGCTTG200.00693264652.49467552
GAATGAC200.00693264652.49467539
ATGGACC200.00693264652.49467525
CGGCATT200.00693264652.49467550
GCGCCGG200.00693264652.49467547
GGTTTAC200.00693264652.49467550
TCGGCAT200.00693264652.49467549
GACCATG200.00693264652.49467528
TCAATGA200.00693264652.49467519
GATGGAC200.00693264652.49467524
CTGCACG200.00693264652.49467519
TACCTGC200.00693264652.49467516
Produced by FastQC (version 0.11.5)