##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780698_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 84460 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.855837082642672 34.0 31.0 34.0 12.0 34.0 2 31.044683874023207 34.0 32.0 34.0 23.0 34.0 3 31.17367984844897 34.0 33.0 34.0 23.0 34.0 4 31.5154747809614 34.0 34.0 34.0 23.0 34.0 5 31.479552450864315 34.0 34.0 34.0 23.0 34.0 6 34.40850106559318 38.0 36.0 38.0 24.0 38.0 7 33.270530428605255 37.0 34.0 38.0 21.0 38.0 8 32.351787828557896 37.0 31.0 38.0 11.0 38.0 9 33.22415344541795 37.0 34.0 38.0 21.0 38.0 10-11 33.60105375325598 37.0 34.0 38.0 21.5 38.0 12-13 33.98601112952877 38.0 34.0 38.0 22.0 38.0 14-15 33.59528771015866 37.0 34.0 38.0 21.0 38.0 16-17 33.445050911674166 37.0 34.0 38.0 21.0 38.0 18-19 33.46939379587971 37.0 34.0 38.0 21.0 38.0 20-21 33.494447075538716 37.0 34.0 38.0 21.0 38.0 22-23 33.47198673928487 37.0 34.0 38.0 21.0 38.0 24-25 33.89575538716552 37.5 34.0 38.0 21.5 38.0 26-27 33.96579445891546 38.0 34.0 38.0 22.0 38.0 28-29 34.11206488278475 38.0 34.5 38.0 23.0 38.0 30-31 33.932737390480696 38.0 34.0 38.0 22.0 38.0 32-33 33.71905043807719 37.5 34.0 38.0 21.5 38.0 34-35 33.563266635093534 37.0 34.0 38.0 21.0 38.0 36-37 33.543340042623726 37.0 34.0 38.0 21.0 38.0 38-39 34.073981766516695 38.0 34.0 38.0 23.0 38.0 40-41 33.5793985318494 37.5 34.0 38.0 21.0 38.0 42-43 33.91304759649539 37.5 34.0 38.0 21.5 38.0 44-45 32.975923514089516 37.0 33.5 38.0 16.0 38.0 46-47 33.69994672034099 37.0 34.0 38.0 21.0 38.0 48-49 33.4267878285579 37.0 34.0 38.0 21.0 38.0 50-51 33.40844186597206 37.0 34.0 38.0 21.0 38.0 52-53 33.40616268055884 37.0 34.0 38.0 21.0 38.0 54-55 33.079759649538246 37.0 33.5 38.0 21.0 38.0 56-57 33.20945417949325 37.0 33.0 38.0 20.5 38.0 58-59 33.08028060620412 37.0 33.0 38.0 16.0 38.0 60-61 33.09104901728629 37.0 32.5 38.0 20.5 38.0 62-63 33.168038124556006 37.0 33.0 38.0 20.0 38.0 64-65 33.17493488041677 37.0 33.0 38.0 20.0 38.0 66-67 33.10936538006157 37.0 32.5 38.0 19.5 38.0 68-69 32.69823585129055 37.0 31.5 38.0 15.0 38.0 70-71 32.53461401847028 37.0 31.0 38.0 11.0 38.0 72-73 32.58152379824769 37.0 31.5 38.0 15.0 38.0 74-75 32.20942457968269 37.0 31.0 38.0 11.0 38.0 76 31.4827847501776 36.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.7325901832343895 1101 2 -1.2688817457139336 1101 3 -0.8243023192720855 1101 4 -2.840969861218362 1101 5 -0.8489579088008767 1101 6 -1.79884197926215 1101 7 -1.3285860590484937 1101 8 -0.5175154993912443 1101 9 -0.8905187887766033 1101 10-11 -1.494334148633115 1101 12-13 -1.5929965777555708 1101 14-15 -0.513026077949327 1101 16-17 -2.5758447899822556 1101 18-19 -1.2174831478184558 1101 20-21 -1.5576576806409435 1101 22-23 -0.3995445095625101 1101 24-25 -1.1686252130552859 1101 26-27 -1.4996445041631716 1101 28-29 -0.8695861203356543 1101 30-31 -1.8627999770796322 1101 32-33 -0.055564868995219285 1101 34-35 -1.7354794243664458 1101 36-37 -1.0195544332673023 1101 38-39 -1.9578594923804928 1101 40-41 -0.7212580154146124 1101 42-43 -0.9809171496529743 1101 44-45 -0.2960159651172347 1101 46-47 0.1375477926722013 1101 48-49 -1.8153437967881203 1101 50-51 -1.6727822237887864 1101 52-53 -1.2329730248748731 1101 54-55 -2.117915116567737 1101 56-57 -0.7655370290041645 1101 58-59 -1.0831250269948356 1101 60-61 -0.10850278447374961 1101 62-63 -2.0379155968965534 1101 64-65 -0.6223051361400067 1101 66-67 -2.2404122447404866 1101 68-69 -0.028835252823867563 1101 70-71 -0.534987707829643 1101 72-73 -1.2398906221992547 1101 74-75 -2.1724685231961587 1101 76 -1.8498841247939808 1102 1 0.3386538875870073 1102 2 -0.25418207915685187 1102 3 0.35902083857321543 1102 4 0.2826153545802548 1102 5 0.21289340383707867 1102 6 -0.014006587349321364 1102 7 1.0193921497525196 1102 8 1.735501924175182 1102 9 0.8073631442530314 1102 10-11 -1.243970179253509 1102 12-13 -4.533540418454393 1102 14-15 -0.9776623433886797 1102 16-17 -2.4330748655939587 1102 18-19 -0.6791489534822901 1102 20-21 -0.4992582066353961 1102 22-23 -1.245550239145004 1102 24-25 0.27386762496659856 1102 26-27 -5.572344452502996 1102 28-29 -0.4174891870712045 1102 30-31 0.8359742679774271 1102 32-33 -6.887152714766128 1102 34-35 -0.9192839610686434 1102 36-37 0.3547234984473491 1102 38-39 0.47412519742366754 1102 40-41 -0.0627434801213198 1102 42-43 -0.17986750892597314 1102 44-45 -6.114817918810935 1102 46-47 0.08559409897005565 1102 48-49 0.5019318337007732 1102 50-51 -6.572655421553307 1102 52-53 -1.7838348104382042 1102 54-55 1.0821741146350021 1102 56-57 -0.8724148761839885 1102 58-59 1.2463964119757236 1102 60-61 0.5159864843000292 1102 62-63 -4.123471882999965 1102 64-65 -0.8595662078537103 1102 66-67 0.18044202661629782 1102 68-69 -7.0475620644521015 1102 70-71 -0.43722083608775364 1102 72-73 -0.11704508610758069 1102 74-75 -6.649726307444503 1102 76 -2.4539558854665025 1103 1 -0.6649375093847922 1103 2 -0.7435915392229653 1103 3 -0.36813501989309216 1103 4 -0.583788759201731 1103 5 -0.3927467218618155 1103 6 -1.4542588013639275 1103 7 -0.4904433805907047 1103 8 0.7894340957460102 1103 9 0.41381949653642636 1103 10-11 -0.25129581284939206 1103 12-13 -2.446938777838959 1103 14-15 -1.5582851242726505 1103 16-17 -1.197654712959583 1103 18-19 -0.8707275356967088 1103 20-21 -0.9834438165607153 1103 22-23 -1.0964460478214946 1103 24-25 -0.7127870703768266 1103 26-27 -2.347310783156388 1103 28-29 -0.5682870485575506 1103 30-31 -0.8755712570603222 1103 32-33 -5.688401541440413 1103 34-35 -1.5877429252771122 1103 36-37 -1.6540917486652518 1103 38-39 -0.6548488057596131 1103 40-41 -0.6498858708489834 1103 42-43 -0.5645448953684493 1103 44-45 -5.579628350186688 1103 46-47 -1.3569333466488516 1103 48-49 -0.40139633019752097 1103 50-51 -0.8939426108770689 1103 52-53 -1.3869854450543713 1103 54-55 -0.5780103525730951 1103 56-57 -0.008959380938506456 1103 58-59 -0.5480369227179906 1103 60-61 -0.013387469909659444 1103 62-63 -0.6192173019282592 1103 64-65 -0.20329962825120873 1103 66-67 -0.7747981480561918 1103 68-69 -3.84767837674039 1103 70-71 -0.8544452575871659 1103 72-73 -0.29195224033359324 1103 74-75 -2.6255966090402225 1103 76 -1.1098959744352008 1104 1 -1.4650448293463043 1104 2 0.6007205028168556 1104 3 0.21681428067681097 1104 4 0.3792413644012598 1104 5 0.31989482946672965 1104 6 -0.3806706139610725 1104 7 1.0508748045917429 1104 8 1.602086749139545 1104 9 0.23683407724843875 1104 10-11 0.6084573512391245 1104 12-13 -0.10789096494576 1104 14-15 0.41692026392956194 1104 16-17 0.05563889965235802 1104 18-19 1.4473558778182039 1104 20-21 1.3339827247759715 1104 22-23 0.7601522794673841 1104 24-25 1.1877754342490334 1104 26-27 1.3089322780529997 1104 28-29 0.0868273027117823 1104 30-31 -0.4238467362994598 1104 32-33 -0.3959638980387368 1104 34-35 0.4988809367612248 1104 36-37 0.44556778800857444 1104 38-39 0.06289044374079111 1104 40-41 1.0848487897978885 1104 42-43 0.6199899281087156 1104 44-45 -2.9548503914863673 1104 46-47 0.9514265082841646 1104 48-49 1.05123485920371 1104 50-51 1.8902980078780232 1104 52-53 0.4740259530656772 1104 54-55 -0.09777202824476916 1104 56-57 0.9408870404958805 1104 58-59 1.319459931696926 1104 60-61 0.42361756302646825 1104 62-63 0.9410576592145077 1104 64-65 0.8779397322698443 1104 66-67 0.4346793147518895 1104 68-69 -3.175947934878206 1104 70-71 0.27323774145065727 1104 72-73 1.104937331126699 1104 74-75 -0.7566287310149136 1104 76 1.0786355924362354 1105 1 -0.44106928761706143 1105 2 0.1467406701629166 1105 3 -0.48484130110105994 1105 4 -0.03815833523401224 1105 5 0.38872841310400474 1105 6 0.07071678502340717 1105 7 1.0840043190020978 1105 8 0.11751619918602785 1105 9 0.49630986928162457 1105 10-11 1.0209850634611115 1105 12-13 -1.2358946122317462 1105 14-15 -0.39964270539032754 1105 16-17 0.811217101754913 1105 18-19 -0.07353556178703258 1105 20-21 -0.646010838836979 1105 22-23 0.31389611126313355 1105 24-25 0.4805595292214946 1105 26-27 0.575827794036698 1105 28-29 -0.3989947669442273 1105 30-31 0.15026110894118716 1105 32-33 0.5711658309178489 1105 34-35 0.7561665693150417 1105 36-37 0.9475702195325653 1105 38-39 0.4338140652812328 1105 40-41 0.6558278357822971 1105 42-43 0.8579395812589681 1105 44-45 2.0388651917218112 1105 46-47 1.2634303701163887 1105 48-49 0.2478307256129142 1105 50-51 0.22911120962299947 1105 52-53 -0.5358163554518143 1105 54-55 -1.0468530552060784 1105 56-57 -0.06965254328443393 1105 58-59 -0.023724649580337598 1105 60-61 0.32239643737388946 1105 62-63 0.3541396158761714 1105 64-65 0.506694291377336 1105 66-67 0.5633534190213609 1105 68-69 1.4655989434740775 1105 70-71 1.6480053163630473 1105 72-73 0.549414553468111 1105 74-75 -0.6610930805822441 1105 76 -0.8603796403464266 1106 1 -0.6055687122430697 1106 2 -0.28340892707528553 1106 3 -0.5391949643154845 1106 4 -0.6035783582489707 1106 5 -0.1574549394084599 1106 6 -0.42458432736631124 1106 7 0.12984244711870474 1106 8 1.3043209556110398 1106 9 1.5735058800219406 1106 10-11 -0.04065667033252396 1106 12-13 0.10297190100185105 1106 14-15 0.06422380784326975 1106 16-17 0.46858188886653807 1106 18-19 -0.4539153086224772 1106 20-21 -0.5449463969497543 1106 22-23 -0.5184686758484887 1106 24-25 0.18896957908724232 1106 26-27 -0.22092134290231513 1106 28-29 -1.5598213875809748 1106 30-31 -0.24437832335647158 1106 32-33 0.6251455010367621 1106 34-35 0.34603039747001674 1106 36-37 -0.03212866807771775 1106 38-39 0.16497056700735868 1106 40-41 -0.45675367553338475 1106 42-43 0.04814863380979517 1106 44-45 0.6125610106707953 1106 46-47 0.34634176252145465 1106 48-49 0.012637016368572063 1106 50-51 -0.07006408765356298 1106 52-53 -0.4654193473965762 1106 54-55 -0.23906632716617793 1106 56-57 -0.018357951799963246 1106 58-59 -0.5925585582876636 1106 60-61 0.4842107833654481 1106 62-63 0.343754115300797 1106 64-65 0.7632247861989683 1106 66-67 0.7940783902526576 1106 68-69 1.4388440527951332 1106 70-71 0.16588026841521497 1106 72-73 -0.18123370123077365 1106 74-75 -1.6033159421089032 1106 76 -0.8725200316007431 1107 1 -0.3686154980932024 1107 2 -0.03852494076870627 1107 3 -0.3056841236916732 1107 4 0.049654810256164694 1107 5 -0.25357513720230074 1107 6 0.1169713972438089 1107 7 0.0029881298878180473 1107 8 0.2944312637129798 1107 9 -0.6858132527281597 1107 10-11 -0.007412090644422165 1107 12-13 0.24143709689383286 1107 14-15 -0.7047377745074286 1107 16-17 0.7280912079992845 1107 18-19 0.5985951516285724 1107 20-21 0.9227884832328215 1107 22-23 -0.7515135598347982 1107 24-25 -0.18390033342701884 1107 26-27 0.1573118256028181 1107 28-29 -1.0906601095284785 1107 30-31 0.2380389455670766 1107 32-33 0.5711040404053378 1107 34-35 0.3584210402999872 1107 36-37 -0.7158392044033164 1107 38-39 0.43413184505984503 1107 40-41 0.457091893123895 1107 42-43 -0.18259499494638476 1107 44-45 -0.43379692010135784 1107 46-47 -0.1747879445915217 1107 48-49 0.36202841994407464 1107 50-51 0.6137130139059579 1107 52-53 0.6121101314927415 1107 54-55 -0.09482897456063455 1107 56-57 0.6773153079946326 1107 58-59 0.7190237924160243 1107 60-61 -0.19131222918684898 1107 62-63 -0.4514056412606351 1107 64-65 0.5852388599912786 1107 66-67 0.953454578917551 1107 68-69 1.130076460554747 1107 70-71 0.17055885342853117 1107 72-73 0.06110178754823181 1107 74-75 -0.27995154499973296 1107 76 -0.6186021921180469 1108 1 -1.259828887214539 1108 2 -0.3523324721494596 1108 3 -0.9040104835243064 1108 4 -0.577960252505342 1108 5 -0.25357513720230074 1108 6 0.14207599975426888 1108 7 0.8272559123145982 1108 8 0.8969417239640265 1108 9 -0.3720057213474064 1108 10-11 -0.14548740445195563 1108 12-13 0.24980529773064575 1108 14-15 0.6153459075009309 1108 16-17 -1.0208627668961157 1108 18-19 -0.215212379752181 1108 20-21 0.4437089853248679 1108 22-23 -1.0590449405879312 1108 24-25 -1.3596325510002458 1108 26-27 -1.3489643250247951 1108 28-29 0.20641102017863489 1108 30-31 -0.13853009208983025 1108 32-33 0.5292630362212449 1108 34-35 0.21406957586484054 1108 36-37 -0.1823664010560293 1108 38-39 1.0136297530096385 1108 40-41 -0.030355805620878584 1108 42-43 0.3906267623757955 1108 44-45 -0.29781365650303115 1108 46-47 0.678768540764132 1108 48-49 0.29926691366792824 1108 50-51 0.5907004616047047 1108 52-53 -0.3711534668336185 1108 54-55 0.38843462376573257 1108 56-57 0.332127023475806 1108 58-59 -1.7998046594668153 1108 60-61 0.8296082729051975 1108 62-63 0.35612573949250503 1108 64-65 0.2170380231711917 1108 66-67 0.3614043697125311 1108 68-69 0.9794488454919801 1108 70-71 1.796081865980831 1108 72-73 0.27867500930555167 1108 74-75 0.7263246056278803 1108 76 0.36047730578989956 1109 1 -0.7700209266952811 1109 2 0.011773668107309732 1109 3 0.5953815702610505 1109 4 -0.14705155172216422 1109 5 0.8642233822698628 1109 6 1.038313885531899 1109 7 -0.2640969468206862 1109 8 1.3571570084470892 1109 9 1.5659301224461828 1109 10-11 1.57410634443049 1109 12-13 0.8992617222916692 1109 14-15 0.45132559494505387 1109 16-17 -0.1565152362305895 1109 18-19 0.2995034947963262 1109 20-21 1.5434568664535036 1109 22-23 0.9696626372828234 1109 24-25 0.9985461136637781 1109 26-27 1.2896652926843188 1109 28-29 0.8316121788525948 1109 30-31 0.96449891052076 1109 32-33 1.3479698988611588 1109 34-35 0.4392510656906836 1109 36-37 0.7150158540668059 1109 38-39 1.008113535150322 1109 40-41 1.2744040556243448 1109 42-43 0.9243141349752975 1109 44-45 0.5450590681688539 1109 46-47 -0.25964114346145806 1109 48-49 0.5418910956226526 1109 50-51 1.5544114368219581 1109 52-53 1.196954389977165 1109 54-55 0.6580587699589202 1109 56-57 0.9793304708884563 1109 58-59 0.30095348522438314 1109 60-61 0.7162620654167355 1109 62-63 -0.2974075008608139 1109 64-65 0.516701864676044 1109 66-67 0.511638607812877 1109 68-69 1.6441665081175927 1109 70-71 0.8816145341494845 1109 72-73 0.19817577817870102 1109 74-75 2.109679395886438 1109 76 -0.9252395343202444 1110 1 -0.331948582372938 1110 2 -0.09517840759476925 1110 3 -0.1693086006791198 1110 4 0.15640540798479563 1110 5 0.08434700864348699 1110 6 0.07582151678953153 1110 7 0.6347125769888322 1110 8 1.4195806958707777 1110 9 -1.892403210887153 1110 10-11 -1.0242605664364177 1110 12-13 -0.5965410860111362 1110 14-15 -0.8875213220268634 1110 16-17 -0.37902363061398603 1110 18-19 0.44474540566323384 1110 20-21 -1.0360989943523577 1110 22-23 -0.1052381563679674 1110 24-25 -0.05409048584782283 1110 26-27 -1.6131697195256933 1110 28-29 0.15681822280863855 1110 30-31 -0.39600169997984835 1110 32-33 1.0392282932445553 1110 34-35 0.34164728058689775 1110 36-37 -0.2338737979478509 1110 38-39 -0.03490767974589204 1110 40-41 0.3376862595315444 1110 42-43 0.5765577247188887 1110 44-45 1.3660106859954766 1110 46-47 0.659734619664313 1110 48-49 0.8810623410438936 1110 50-51 0.07518753572305314 1110 52-53 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0.2906797734807256 2116 38-39 0.5030387488255386 2116 40-41 0.4202755452458362 2116 42-43 0.9828077247188887 2116 44-45 2.343689257424046 2116 46-47 1.3115203339500212 2116 48-49 1.0417766267581854 2116 50-51 1.3497411071516296 2116 52-53 1.266398834421608 2116 54-55 0.6173460104961563 2116 56-57 0.19970211313510333 2116 58-59 -0.22510725958636613 2116 60-61 -0.8872177880631185 2116 62-63 0.8459456737423565 2116 64-65 1.214198812172988 2116 66-67 0.4572277409020131 2116 68-69 0.7077158060418896 2116 70-71 -2.868900575740625 2116 72-73 1.1938068831848128 2116 74-75 -1.2865545784725398 2116 76 -0.6733317199124293 2117 1 -0.10142551031225722 2117 2 0.6490930841877862 2117 3 0.9421405143651249 2117 4 0.35816741809857433 2117 5 -0.5169883263754755 2117 6 -0.755839355523996 2117 7 -3.044332935021277 2117 8 -2.356177837631119 2117 9 0.2325967891128471 2117 10-11 -0.5760146751595556 2117 12-13 0.6415278141152854 2117 14-15 -0.14832647373355456 2117 16-17 -0.04710596310449944 2117 18-19 0.04033247898814096 2117 20-21 -0.01962459485804402 2117 22-23 0.751865193821665 2117 24-25 0.9028271816616638 2117 26-27 0.9414881502593886 2117 28-29 0.517793254414201 2117 30-31 -0.34258829922131184 2117 32-33 1.0261712452040967 2117 34-35 0.2300006307765301 2117 36-37 -0.666139126645696 2117 38-39 0.4496648550202238 2117 40-41 -1.2059491703041019 2117 42-43 -1.5722254611218247 2117 44-45 0.41193751911809784 2117 46-47 -0.3691449631422685 2117 48-49 -0.24050055402563686 2117 50-51 1.268672839136464 2117 52-53 1.5551156485808946 2117 54-55 0.014272369535341056 2117 56-57 -0.5061093596841175 2117 58-59 -0.2497991053512152 2117 60-61 0.8213789249583741 2117 62-63 0.31050793670063825 2117 64-65 -0.04528285660071418 2117 66-67 -0.8217703627642337 2117 68-69 -0.7377448450326973 2117 70-71 1.1396131726790912 2117 72-73 0.773472654360539 2117 74-75 0.2879752887840965 2117 76 -0.49894802838272057 2118 1 0.0894989860161175 2118 2 0.26281931276997383 2118 3 0.030824378044282952 2118 4 0.27253382743768384 2118 5 -0.4176096782866061 2118 6 0.38537698791415664 2118 7 1.1323569538885323 2118 8 -3.9900697600562722 2118 9 -0.403573423653107 2118 10-11 -0.2777940010868747 2118 12-13 1.0488616495511707 2118 14-15 -0.8659502733946383 2118 16-17 -0.2574335850212748 2118 18-19 -0.689021691601198 2118 20-21 -2.845768447239891 2118 22-23 -0.45787873387556743 2118 24-25 0.1265421129363773 2118 26-27 0.5652972257630609 2118 28-29 0.1640180708329524 2118 30-31 -0.2624150738400317 2118 32-33 0.324420162545465 2118 34-35 0.4713205328665282 2118 36-37 -0.013898493996478578 2118 38-39 -0.05161550649361146 2118 40-41 -1.1143939076417055 2118 42-43 -0.3263678071960072 2118 44-45 -0.1229805754027069 2118 46-47 -1.0547220672657858 2118 48-49 -1.2491618230898425 2118 50-51 -0.5131008381371274 2118 52-53 -1.5692394634507316 2118 54-55 -0.05125201381995481 2118 56-57 0.8068354717977115 2118 58-59 0.459182254091445 2118 60-61 -2.1849381528047616 2118 62-63 -0.23511435665277958 2118 64-65 -0.3710595464896258 2118 66-67 -1.69296222870285 2118 68-69 0.566406915464384 2118 70-71 -0.9153576426099264 2118 72-73 -2.8475779952346443 2118 74-75 -0.438083833791687 2118 76 -0.5701022366297543 2119 1 0.2790633223889678 2119 2 0.16464302097666206 2119 3 -0.05174907686959429 2119 4 0.37456016988955554 2119 5 0.528692246738725 2119 6 1.241000088218108 2119 7 -1.4545731372968795 2119 8 1.8398187911088755 2119 9 -0.03406987755381863 2119 10-11 0.25371562403977066 2119 12-13 0.48143510446505644 2119 14-15 -0.7965987029792387 2119 16-17 -0.291374821090173 2119 18-19 -0.7158200705272364 2119 20-21 -1.3602061372094951 2119 22-23 1.1593451769653669 2119 24-25 0.22760594272360635 2119 26-27 0.41078861380763954 2119 28-29 1.0089015561419714 2119 30-31 0.3682840143058641 2119 32-33 1.056639007530272 2119 34-35 0.7374806139202263 2119 36-37 -0.24176070270975458 2119 38-39 0.1003601773969649 2119 40-41 -0.10502207380178419 2119 42-43 -0.26451370385253625 2119 44-45 -1.110923837814049 2119 46-47 -0.5227058565261657 2119 48-49 -0.6507828970513359 2119 50-51 0.900931583342107 2119 52-53 -0.9014583084355365 2119 54-55 -0.7037849418847983 2119 56-57 0.5239580655160552 2119 58-59 1.3370951213660192 2119 60-61 0.4175441166987852 2119 62-63 0.0010051975518763356 2119 64-65 0.29991309788727705 2119 66-67 0.5066325028067737 2119 68-69 -0.5031472891962068 2119 70-71 -1.2116089090739592 2119 72-73 -1.6968181168151943 2119 74-75 -0.11467957847253984 2119 76 -0.16321267229338332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 16.0 13 113.0 14 315.0 15 597.0 16 844.0 17 959.0 18 1058.0 19 1019.0 20 955.0 21 825.0 22 735.0 23 774.0 24 917.0 25 964.0 26 1155.0 27 1405.0 28 1555.0 29 1974.0 30 2431.0 31 3013.0 32 3794.0 33 5061.0 34 6580.0 35 10019.0 36 16700.0 37 20682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.20224316907291 25.902636916835696 11.364992244362247 27.530127669729147 2 17.041202936301207 22.60478332938669 36.70613308074828 23.647880653563817 3 15.790906938195596 27.02225905754203 30.44991712053043 26.736916883731944 4 11.83400426237272 19.23395690267582 39.08003788775752 29.85200094719394 5 11.438550793274924 37.56689557186834 36.97726734548899 14.017286289367748 6 25.327965901018235 41.43855079327493 17.57281553398058 15.660667771726262 7 24.360644091877813 30.03433578025101 23.665640539900544 21.939379587970638 8 21.49538242955245 35.67605967321809 20.775515036703766 22.053042860525693 9 22.886573525929435 16.115320861946483 22.467440208382666 38.530665403741416 10-11 23.749112005683163 25.999881600757757 27.620175230878523 22.630831162680558 12-13 22.918541321335542 24.033862183282025 26.498342410608572 26.54925408477386 14-15 20.941273975846556 25.560028415818138 23.927894861472886 29.57080274686242 16-17 21.792564527587025 27.63142315889178 25.80156286999763 24.77444944352356 18-19 20.932394032678193 27.147170258110346 26.325479516931093 25.59495619228037 20-21 20.980345725787355 27.22116978451338 26.435590812218802 25.362893677480464 22-23 22.301681269239875 27.119346436182806 26.616149656642197 23.962822637935115 24-25 21.733364906464598 28.255387165522137 25.872010419133318 24.139237508879944 26-27 20.20068671560502 28.026876627989584 27.373312810798012 24.399123845607388 28-29 21.776580629883966 26.943523561449208 28.173691688373193 23.10620412029363 30-31 21.24852000947194 27.30760123135212 27.486976083353063 23.956902675822874 32-33 20.044399715841816 28.51290551740469 27.09270660667772 24.349988160075775 34-35 21.37520719867393 27.98602888941511 27.247217617807244 23.39154629410372 36-37 22.1631541558134 27.233009708737864 26.68718920198911 23.916646933459624 38-39 21.18458441865972 27.494672034098983 27.85519772673455 23.465545820506748 40-41 21.336135448733128 26.63035756571158 27.978332938669194 24.055174046886098 42-43 20.76249112005683 26.350343357802508 28.278475017759884 24.608690504380775 44-45 19.924224484963297 26.497158418186125 28.453113900071042 25.12550319677954 46-47 20.406701397111057 26.311863604072933 29.063461993843237 24.217973004972766 48-49 20.075775515036703 26.59720577788302 29.10194174757282 24.22507695950746 50-51 19.811153208619466 26.703765095903385 28.251243192043574 25.233838503433574 52-53 19.06760596732181 26.855316125976792 28.642552687662796 25.4345252190386 54-55 18.96696661141369 27.16966611413687 28.653208619464838 25.21015865498461 56-57 19.145157470992185 26.86834004262373 28.966966611413685 25.0195358749704 58-59 19.52758702344305 26.75467677006867 27.91558134027942 25.802154866208856 60-61 19.516931091641013 26.396519062278003 27.772318257163153 26.31423158891783 62-63 19.15818138763912 26.504262372720817 28.153563817191568 26.183992422448497 64-65 20.253966374615203 26.541558134027945 27.073170731707318 26.131304759649538 66-67 20.42505327965901 26.70554108453706 26.513142315889176 26.356263319914753 68-69 20.194766753492775 26.627989580866686 26.22780014207909 26.94944352356145 70-71 21.221288183755625 26.518470281790197 26.406582997868817 25.853658536585368 72-73 21.805588444233955 26.17629647170258 25.7838029836609 26.23431210040256 74-75 21.51728628936775 26.71678901255032 25.822282737390477 25.943641960691448 76 22.18209803457258 25.873786407766993 25.846554582050672 26.097560975609756 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 2.0 14 3.5 15 3.0 16 4.5 17 7.0 18 4.5 19 4.0 20 12.0 21 18.0 22 20.5 23 36.0 24 69.0 25 89.0 26 141.0 27 225.0 28 349.0 29 441.0 30 494.0 31 756.0 32 1042.5 33 1120.0 34 1338.0 35 1851.5 36 2349.5 37 2552.0 38 2937.0 39 3693.0 40 4473.5 41 5208.5 42 5534.0 43 5783.5 44 6160.0 45 6345.5 46 6404.0 47 6382.5 48 6237.5 49 5775.5 50 5437.0 51 4963.5 52 4063.5 53 3333.5 54 3030.0 55 2804.0 56 2182.5 57 1582.0 58 1377.0 59 1162.5 60 816.5 61 573.0 62 461.0 63 388.0 64 272.5 65 184.0 66 120.5 67 103.0 68 88.0 69 60.5 70 36.5 71 25.0 72 23.0 73 19.5 74 15.5 75 13.0 76 11.5 77 8.5 78 6.5 79 6.0 80 4.0 81 3.0 82 4.0 83 4.0 84 3.5 85 3.0 86 2.5 87 2.0 88 2.0 89 1.0 90 0.5 91 2.0 92 3.0 93 2.0 94 1.0 95 1.0 96 1.0 97 1.5 98 1.0 99 4.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7695950745915227 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 84460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.14965664219749 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8570634939006 38.28913094956192 2 8.562301798569477 8.759175941273977 3 4.911923335107984 7.537295761307128 4 3.3425152195551027 6.8387402320625155 5 2.0161570334020045 5.156286999763202 6 1.3934862618920858 4.276580629883968 7 0.9721997175991296 3.480937721998579 8 0.849517372283049 3.476201752308785 9 0.6296150552070554 2.898413450153919 >10 2.458276428786371 19.068197963533034 >50 0.00694428369713664 0.21903859815297183 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005919962112242482 0.0 2 0.0 0.0 0.0 0.00947193937958797 0.0 3 0.0 0.0 0.0 0.014207909069381956 0.0 4 0.0 0.0 0.0 0.01894387875917594 0.0 5 0.0 0.0 0.0 0.021311863604072934 0.0 6 0.0 0.0 0.0 0.021311863604072934 0.0 7 0.0 0.0 0.0 0.023679848448969927 0.0 8 0.0 0.0 0.0 0.02604783329386692 0.0 9 0.0 0.0 0.0 0.07459152261425527 0.0 10 0.0 0.0 0.0 0.10182334833057069 0.0 11 0.0 0.0 0.0 0.11366327255505565 0.0 12 0.0 0.0 0.0 0.11721524982240114 0.0 13 0.0 0.0 0.0 0.11958323466729813 0.0 14 0.0 0.0 0.0 0.12076722708974663 0.0 15 0.0 0.0 0.0 0.13852711342647406 0.0 16 0.0 0.0 0.0 0.15391901491830454 0.0 17 0.0 0.0 0.0 0.159838977030547 0.0 18 0.0 0.0 0.0 0.17286289367748045 0.0 19 0.0 0.0 0.0 0.18351882547951692 0.0 20 0.0 0.0 0.0 0.20719867392848687 0.0 21 0.0 0.0 0.0 0.21075065119583233 0.0 22 0.0 0.0 0.0 0.21311863604072934 0.0 23 0.0 0.0 0.0 0.2237745678427658 0.0 24 0.0 0.0 0.0 0.23324650722235377 0.0 25 0.0 0.0 0.0 0.23561449206725077 0.0 26 0.0 0.0 0.0 0.2581103480937722 0.0 27 0.0 0.0 0.0 0.29836609045702106 0.0 28 0.0 0.0 0.0 0.33033388586313045 0.0 29 0.0 0.0 0.0 0.3563817191569974 0.0 30 0.0 0.0 0.0 0.3990054463651433 0.0 31 0.0 0.0 0.0 0.42742126450390716 0.0 32 0.0 0.0 0.0 0.4558370826426711 0.0 33 0.0 0.0 0.0 0.4878048780487805 0.0 34 0.0 0.0 0.0 0.5363485673691688 0.0 35 0.0 0.0 0.0 0.636987923277291 0.0 36 0.0 0.0 0.0 0.7897229457731471 0.0 37 0.0 0.0 0.0 1.0798010892730288 0.0 38 0.0 0.0 0.0 1.560502012787118 0.0 39 0.0 0.0 0.0 2.1572341937011603 0.0 40 0.0 0.0 0.0 2.7444944352356146 0.0 41 0.0 0.0 0.0 3.4773857447312335 0.0 42 0.0 0.0 0.0 4.172389296708501 0.0 43 0.0 0.0 0.0 4.8413450153919015 0.0 44 0.0 0.0 0.0 5.520956665877338 0.0 45 0.0 0.0 0.0 6.199384323940326 0.0 46 0.0 0.0 0.0 6.931091641013498 0.0 47 0.0 0.0 0.0 7.663982950509117 0.0 48 0.0 0.0 0.0 8.40161022969453 0.0 49 0.0 0.0 0.0 9.097797774094246 0.0 50 0.0 0.0 0.0 9.738337674638883 0.0 51 0.0 0.0 0.0 10.403741416054936 0.0 52 0.0 0.0 0.0 11.118872839213829 0.0 53 0.0 0.0 0.0 11.78782855789723 0.0 54 0.0 0.0 0.0 12.4804641250296 0.0 55 0.0 0.0 0.0 13.080748283210987 0.0 56 0.0 0.0 0.0 13.63012076722709 0.0 57 0.0 0.0 0.0 14.224484963296234 0.0 58 0.0 0.0 0.0 14.801089273028653 0.0 59 0.0 0.0 0.0 15.279422211697845 0.0 60 0.0 0.0 0.0 15.775515036703766 0.0 61 0.0 0.0 0.0 16.34738337674639 0.0 62 0.0 0.0 0.0 16.9310916410135 0.0 63 0.0 0.0 0.0 17.484016102296945 0.0 64 0.0 0.0 0.0 18.004972768174284 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAACTA 15 0.002218647 69.99289 69 CGGTTTA 15 0.002218647 69.99289 49 CTAAATT 15 0.002218647 69.99289 65 CCGGTGA 15 0.002218647 69.99289 50 TGACTGC 25 2.379523E-4 55.994316 42 GCCATAG 20 0.006739729 52.870308 1 CGGCGGC 20 0.006932646 52.494675 22 ACTGCGC 20 0.006932646 52.494675 44 GAGCTTG 20 0.006932646 52.494675 52 GAATGAC 20 0.006932646 52.494675 39 ATGGACC 20 0.006932646 52.494675 25 CGGCATT 20 0.006932646 52.494675 50 GCGCCGG 20 0.006932646 52.494675 47 GGTTTAC 20 0.006932646 52.494675 50 TCGGCAT 20 0.006932646 52.494675 49 GACCATG 20 0.006932646 52.494675 28 TCAATGA 20 0.006932646 52.494675 19 GATGGAC 20 0.006932646 52.494675 24 CTGCACG 20 0.006932646 52.494675 19 TACCTGC 20 0.006932646 52.494675 16 >>END_MODULE