FastQCFastQC Report
Wed 25 May 2016
SRR1780698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84460
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT15291.8103244139237509No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6570.7778830215486621Illumina PCR Primer Index 10 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2310.27350224958560265No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1870.2214065829978688No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1170.13852711342647409No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTAT207.886222E-570.000011
TCATACC150.00221775470.023
CCACGAC150.00221775470.09
AATTTAC352.9428338E-760.053
CGCCATT352.9428338E-760.017
GAGGCGC352.9428338E-760.013
GTCTCGA252.3783301E-455.99999660
TCTCGAG252.3783301E-455.99999661
ACGTCTC252.3783301E-455.99999658
CTCGAGT252.3783301E-455.99999662
GATGTTA200.00692986452.50000438
ATGATTC407.4013224E-752.50000440
CGAGTGA200.00692986452.50000464
ATGGCTC200.00692986452.5000047
TGAAGGT407.4013224E-752.50000428
CGACCAA200.00692986452.50000412
CGTTAAC200.00692986452.5000041
CGTAGTG200.00692986452.50000441
GAGCGTC200.00692986452.50000428
AACTGTA200.00692986452.5000047
Produced by FastQC (version 0.11.5)