##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780698_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 84460 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.0764977504144 34.0 34.0 34.0 32.0 34.0 2 33.399372484016105 34.0 34.0 34.0 33.0 34.0 3 33.526580629883966 34.0 34.0 34.0 34.0 34.0 4 33.54619938432394 34.0 34.0 34.0 34.0 34.0 5 33.54690977977741 34.0 34.0 34.0 34.0 34.0 6 37.310513852711345 38.0 38.0 38.0 36.0 38.0 7 36.920577788302154 38.0 37.0 38.0 35.0 38.0 8 36.972034098981766 38.0 37.0 38.0 35.0 38.0 9 36.774248164811745 38.0 37.0 38.0 34.0 38.0 10-11 36.49941392375089 38.0 37.0 38.0 34.0 38.0 12-13 36.36950035519773 38.0 37.0 38.0 34.0 38.0 14-15 36.60763083116268 38.0 37.0 38.0 34.0 38.0 16-17 36.74824769121477 38.0 37.0 38.0 34.5 38.0 18-19 36.74443523561449 38.0 37.0 38.0 34.0 38.0 20-21 36.48663864551267 38.0 37.0 38.0 34.0 38.0 22-23 36.58415226142553 38.0 37.0 38.0 34.0 38.0 24-25 35.95819322756334 38.0 36.5 38.0 32.5 38.0 26-27 36.13877575183518 38.0 37.0 38.0 33.5 38.0 28-29 36.02934525219038 38.0 37.0 38.0 33.0 38.0 30-31 35.77073762727919 38.0 36.5 38.0 32.0 38.0 32-33 36.2332701870708 38.0 37.0 38.0 34.0 38.0 34-35 36.27997276817428 38.0 37.0 38.0 34.0 38.0 36-37 36.41367511247928 38.0 37.0 38.0 34.0 38.0 38-39 36.01982003315179 38.0 37.0 38.0 32.5 38.0 40-41 35.635549372484014 38.0 36.5 38.0 31.5 38.0 42-43 36.01766516694293 38.0 36.5 38.0 32.5 38.0 44-45 36.202012787118164 38.0 37.0 38.0 34.0 38.0 46-47 36.45382429552451 38.0 37.0 38.0 34.0 38.0 48-49 36.25887402320625 38.0 37.0 38.0 34.0 38.0 50-51 36.41699029126214 38.0 37.0 38.0 34.0 38.0 52-53 36.34826545110111 38.0 37.0 38.0 34.0 38.0 54-55 36.502794222116975 38.0 37.0 38.0 34.0 38.0 56-57 36.54511011129529 38.0 37.0 38.0 34.0 38.0 58-59 36.585146815060384 38.0 37.0 38.0 34.0 38.0 60-61 36.29425763675113 38.0 37.0 38.0 34.0 38.0 62-63 36.556032441392375 38.0 37.0 38.0 34.0 38.0 64-65 36.56046057305234 38.0 37.0 38.0 34.0 38.0 66-67 35.98654392611887 38.0 37.0 38.0 33.0 38.0 68-69 35.40738219275397 38.0 36.0 38.0 30.0 38.0 70-71 35.35925882074355 38.0 36.0 38.0 30.0 38.0 72-73 35.0142611887284 38.0 36.0 38.0 26.0 38.0 74-75 35.55354605730523 38.0 36.5 38.0 31.0 38.0 76 35.04894624674402 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24778137603941985 1101 2 0.4464122471737255 1101 3 0.21620774141367605 1101 4 0.29345906374530273 1101 5 0.3726740572437919 1101 6 -1.0978267681205125 1101 7 0.07672349065829565 1101 8 0.5906234688193095 1101 9 0.3695661787563367 1101 10-11 0.3670194863813592 1101 12-13 0.564290439805454 1101 14-15 0.771190389803941 1101 16-17 0.1178389934055204 1101 18-19 -0.1407797727701947 1101 20-21 0.10556876516487534 1101 22-23 0.6156690959971982 1101 24-25 -1.8584705678220885 1101 26-27 -0.5378834644500117 1101 28-29 0.7318551005787768 1101 30-31 0.928235391215992 1101 32-33 0.33491248909938065 1101 34-35 0.6949089925181084 1101 36-37 0.41342896990194333 1101 38-39 0.4354779985850641 1101 40-41 1.4121687256171285 1101 42-43 0.55502455075505 1101 44-45 0.23535342011479088 1101 46-47 0.40355459677247296 1101 48-49 -0.9098237582327116 1101 50-51 -1.3459142132274877 1101 52-53 -1.6424440049097342 1101 54-55 -0.6492603683407978 1101 56-57 -1.2494991144953147 1101 58-59 -0.6733089405487931 1101 60-61 -1.6253653781421846 1101 62-63 -0.37369616891001556 1101 64-65 -0.539373143956233 1101 66-67 -0.8843093789137342 1101 68-69 -0.9153208956162544 1101 70-71 -0.8134267460499842 1101 72-73 -1.3370835937842642 1101 74-75 -0.7953912103571597 1101 76 -0.7591795407397157 1102 1 -1.096486693279136 1102 2 0.26574254351524473 1102 3 0.19176308824127375 1102 4 0.25121513381527905 1102 5 -0.2144437749076218 1102 6 0.12419455344056018 1102 7 -0.07757604440017474 1102 8 0.276881863596941 1102 9 0.2252464492677717 1102 10-11 0.6901421088394457 1102 12-13 0.551809812369207 1102 14-15 -0.01510087962732598 1102 16-17 -0.11224835924557652 1102 18-19 -0.4733890809935488 1102 20-21 0.4328946469100501 1102 22-23 0.4586574020132659 1102 24-25 -4.64932906592006 1102 26-27 -0.9205679147388324 1102 28-29 -0.027114983486406175 1102 30-31 1.2725504243254662 1102 32-33 0.1682379951342341 1102 34-35 0.8591648512510233 1102 36-37 -2.3386139550029625 1102 38-39 -0.35107157169625935 1102 40-41 0.8085313800105922 1102 42-43 0.6321743182842852 1102 44-45 0.8732860740386172 1102 46-47 0.8773135785974873 1102 48-49 -3.1847850131051985 1102 50-51 -0.3684333509722819 1102 52-53 0.0708537924884709 1102 54-55 0.5890456477208161 1102 56-57 0.6919840020218615 1102 58-59 -0.07783155361411787 1102 60-61 -6.279207016239212 1102 62-63 -2.589999648926927 1102 64-65 -0.09879408041422977 1102 66-67 0.727147437880511 1102 68-69 -1.3949503513059227 1102 70-71 -1.7610503782295694 1102 72-73 -6.358757383292556 1102 74-75 -2.2918806669860956 1102 76 -0.4600906382835106 1103 1 0.613079482291198 1103 2 0.532322146012902 1103 3 0.14868673024429313 1103 4 0.336776085535476 1103 5 0.21421802160708836 1103 6 0.33023054903294025 1103 7 0.6573339666188858 1103 8 0.6417963638622126 1103 9 0.2919729725506315 1103 10-11 -0.09456792666640723 1103 12-13 -0.5158043573242992 1103 14-15 -0.3403059565566835 1103 16-17 0.0808198364855528 1103 18-19 0.5694526118603846 1103 20-21 -0.32632871349479586 1103 22-23 0.41610138846390754 1103 24-25 -3.124791254223531 1103 26-27 -0.38849020996799055 1103 28-29 -0.0949536154906454 1103 30-31 0.7529813919620736 1103 32-33 0.9622632165356961 1103 34-35 0.1503740909359621 1103 36-37 -0.7759787674749035 1103 38-39 -0.5433133380105843 1103 40-41 -0.45620150983962304 1103 42-43 0.09004682369179307 1103 44-45 1.0521009130379184 1103 46-47 0.8064753138952057 1103 48-49 -2.1202316931035696 1103 50-51 -0.519006750147895 1103 52-53 0.30424358149389263 1103 54-55 0.10835960927654043 1103 56-57 0.3747575399559864 1103 58-59 -0.5821983658411938 1103 60-61 -3.9652964746728756 1103 62-63 -0.9286214503389942 1103 64-65 0.04389252938865695 1103 66-67 -0.90962152918474 1103 68-69 -0.18074188703624117 1103 70-71 0.2320627099637136 1103 72-73 -4.94964421347661 1103 74-75 -2.3914501074627665 1103 76 -3.112847443572658 1104 1 -0.11462387053242651 1104 2 -0.10543597699798113 1104 3 0.15390470078681773 1104 4 -0.06741309365185799 1104 5 -0.46907707128018217 1104 6 0.11346273021864306 1104 7 0.5149314797563278 1104 8 0.20892685013265577 1104 9 0.7778363431375652 1104 10-11 -0.35840850101323696 1104 12-13 0.2827024149353434 1104 14-15 0.42278522840371835 1104 16-17 0.09274530462263186 1104 18-19 0.6953500287799343 1104 20-21 -0.2720396157226119 1104 22-23 -0.17634180597026017 1104 24-25 0.3700555372796899 1104 26-27 0.6297449317683714 1104 28-29 0.8540231441866482 1104 30-31 0.5545874906964414 1104 32-33 0.4053281266089641 1104 34-35 0.11173890479133775 1104 36-37 0.9063171395638037 1104 38-39 1.2455346377129004 1104 40-41 1.5046544593706486 1104 42-43 0.33168326807044224 1104 44-45 0.44726041270486405 1104 46-47 0.5524878222217566 1104 48-49 -0.03020319780343783 1104 50-51 0.23775625777157217 1104 52-53 0.27588705718037687 1104 54-55 0.1555156302964491 1104 56-57 -0.025582923370187416 1104 58-59 -0.5291027400547961 1104 60-61 0.1638667996961587 1104 62-63 0.08232333539114478 1104 64-65 0.33661697613575114 1104 66-67 -0.10379664575642522 1104 68-69 1.641541807730981 1104 70-71 1.5363518077358975 1104 72-73 0.9022912465189492 1104 74-75 0.39438180727336913 1104 76 -0.41534170750574617 1105 1 0.6577425744364689 1105 2 0.2983936732916561 1105 3 0.2986876515126937 1105 4 -0.34382015937451627 1105 5 -0.22885680381254758 1105 6 0.06545801020147479 1105 7 0.572429870659569 1105 8 -0.34871457159372454 1105 9 0.6236848734958542 1105 10-11 0.15791489167788342 1105 12-13 -0.9078593386225506 1105 14-15 6.923298840959546E-4 1105 16-17 -0.3368924698177622 1105 18-19 0.42791814720946064 1105 20-21 -0.06593219957442642 1105 22-23 -0.07035596602175076 1105 24-25 0.29490177914480853 1105 26-27 0.11176774518465749 1105 28-29 0.4009283147509066 1105 30-31 0.9075518045060633 1105 32-33 0.6726348562091999 1105 34-35 -1.3351251444112648 1105 36-37 0.06107649020386674 1105 38-39 -0.21549840240724194 1105 40-41 0.4028075738668164 1105 42-43 0.22700258438310783 1105 44-45 0.5605765823394151 1105 46-47 0.6030223999232476 1105 48-49 -0.23597985517310605 1105 50-51 0.3678338519931259 1105 52-53 0.10862144894180403 1105 54-55 0.4058463890749735 1105 56-57 0.41945748283734474 1105 58-59 0.09938363625930435 1105 60-61 0.8751859014625936 1105 62-63 -0.18198868634953413 1105 64-65 0.28630588409541957 1105 66-67 0.15179943519901684 1105 68-69 -0.06562940016431895 1105 70-71 0.42916808464357104 1105 72-73 0.5250116927751165 1105 74-75 -0.3461513401198957 1105 76 0.7761765646819114 1106 1 -1.204685503814396 1106 2 -0.13417249471754644 1106 3 0.285645841002534 1106 4 0.1104759664405961 1106 5 -0.15960095417695186 1106 6 0.05907135887811421 1106 7 -0.8806771489031462 1106 8 0.13881899188920954 1106 9 -0.7196983255835434 1106 10-11 0.2579628395106468 1106 12-13 1.1468395489877352 1106 14-15 -0.5927771752942803 1106 16-17 0.0731382167952006 1106 18-19 0.4912668238492657 1106 20-21 0.300418011396026 1106 22-23 0.2877568072808927 1106 24-25 -0.6368396861409593 1106 26-27 0.5964244387021225 1106 28-29 0.5819122042790994 1106 30-31 0.8639480453959791 1106 32-33 0.3933866782268467 1106 34-35 0.8731410619562112 1106 36-37 0.8933741503496364 1106 38-39 -0.6602049616707646 1106 40-41 0.8396698676911356 1106 42-43 0.5791794159903247 1106 44-45 0.6895562524583312 1106 46-47 0.5314554647487171 1106 48-49 1.0120926419500265 1106 50-51 -0.29749027535631 1106 52-53 0.32030154100164054 1106 54-55 -0.16154908464961437 1106 56-57 0.2444478932707952 1106 58-59 0.3789866282040606 1106 60-61 0.46240300924932143 1106 62-63 0.5221923646669566 1106 64-65 -1.0462027465144814 1106 66-67 0.11338363159334364 1106 68-69 0.9669655365445422 1106 70-71 0.36108216212726774 1106 72-73 -0.2652357180419145 1106 74-75 -0.7756104885663859 1106 76 -0.3891475626675387 1107 1 0.4634202398143614 1107 2 -0.1425787328308985 1107 3 0.15784059185837407 1107 4 0.31532571919829877 1107 5 0.1953438917720831 1107 6 0.20914743343117692 1107 7 0.7780333874224539 1107 8 0.12740780892990955 1107 9 -0.4255180288927889 1107 10-11 0.49768326552814557 1107 12-13 0.14676347443467108 1107 14-15 -0.11323172074882137 1107 16-17 0.5468354400740125 1107 18-19 0.5378434689614764 1107 20-21 0.7072171745759448 1107 22-23 -0.04932953733683121 1107 24-25 0.3766064911127458 1107 26-27 -0.042982179783997765 1107 28-29 0.6609251369531179 1107 30-31 0.2991800727828178 1107 32-33 0.8637176025326596 1107 34-35 -0.0586390825854437 1107 36-37 0.7139903542294306 1107 38-39 0.8384447150123862 1107 40-41 0.7100388292734934 1107 42-43 0.7273821546742312 1107 44-45 0.5835273441281643 1107 46-47 0.5835760429153254 1107 48-49 0.6505198005654691 1107 50-51 0.8443887661043163 1107 52-53 0.7155278627970034 1107 54-55 0.6993866323530469 1107 56-57 0.4992025528371684 1107 58-59 0.3698671911557554 1107 60-61 1.037288897419721 1107 62-63 0.6265666514680248 1107 64-65 0.682762639563876 1107 66-67 -0.16769282333248015 1107 68-69 1.1444759967955918 1107 70-71 0.43924876539847446 1107 72-73 1.0142336619504775 1107 74-75 -0.41741345547395525 1107 76 0.5811833616382813 1108 1 0.08266710173904812 1108 2 0.2841995098469283 1108 3 0.1620246922767805 1108 4 0.3195098196167052 1108 5 -0.0975431375166238 1108 6 -0.1297647004600364 1108 7 -0.05460259584114624 1108 8 -0.7638055801914305 1108 9 -0.3041791167588954 1108 10-11 0.384712554231065 1108 12-13 0.8915333489116577 1108 14-15 0.2717055177448984 1108 16-17 -0.05567502017702708 1108 18-19 0.32654639783176975 1108 20-21 1.0879703126512652 1108 22-23 0.26238594383472247 1108 24-25 1.3452257379746726 1108 26-27 0.18505129301934886 1108 28-29 -0.5022547793648684 1108 30-31 0.33265287613010486 1108 32-33 0.8741778535786864 1108 34-35 0.7949174027702028 1108 36-37 1.1470447475348706 1108 38-39 1.4493233761002458 1108 40-41 1.2748923857588466 1108 42-43 0.25248675718468405 1108 44-45 0.9308076788561976 1108 46-47 0.8806471726224387 1108 48-49 1.1651641520299023 1108 50-51 0.9029661719620563 1108 52-53 0.24481656572586985 1108 54-55 -0.24203596178921316 1108 56-57 0.24188037710495536 1108 58-59 0.2966454338335822 1108 60-61 0.9828955919803946 1108 62-63 0.8797047267818314 1108 64-65 0.5593316772207757 1108 66-67 0.6816795616047528 1108 68-69 0.8118400135319916 1108 70-71 -1.0921319877396058 1108 72-73 -0.06317219579010924 1108 74-75 -0.6098820747208151 1108 76 0.6816017716801284 1109 1 -0.918477267606157 1109 2 0.2560372954922414 1109 3 0.19823325358994737 1109 4 -0.003238097273467133 1109 5 0.01868187410588007 1109 6 -0.2722029223961684 1109 7 -0.3536763719023668 1109 8 0.13337998645020122 1109 9 0.4661602602750392 1109 10-11 0.11279966934747421 1109 12-13 0.20717365932184606 1109 14-15 0.4252298177127187 1109 16-17 0.5955158391728261 1109 18-19 0.6451906777731224 1109 20-21 0.9151616011396229 1109 22-23 0.44016550968959933 1109 24-25 -0.09950479880607332 1109 26-27 0.294365386643058 1109 28-29 -1.0422719449050462 1109 30-31 0.21140336785130387 1109 32-33 0.5668521016922625 1109 34-35 -0.26726297844783176 1109 36-37 0.9368473438228264 1109 38-39 0.7435246420588371 1109 40-41 0.4310451590664286 1109 42-43 1.1056945675054664 1109 44-45 0.6493464622485376 1109 46-47 0.7877297460230039 1109 48-49 0.662267467124849 1109 50-51 0.19648796862193052 1109 52-53 -0.06478004407993865 1109 54-55 0.07792255482202393 1109 56-57 -0.41639126756836475 1109 58-59 -0.24449822028078927 1109 60-61 0.8405110123573252 1109 62-63 -0.15364291116831197 1109 64-65 0.3274180771063442 1109 66-67 0.20538052359023595 1109 68-69 -0.4729917284127154 1109 70-71 0.1466299476750521 1109 72-73 0.02977205196585686 1109 74-75 -0.19103395398985157 1109 76 0.2608135872936117 1110 1 -0.8450264129053053 1110 2 -0.7281660012110578 1110 3 -0.032535977179286135 1110 4 0.0312551872198128 1110 5 0.21759060426460763 1110 6 -0.16544162813487162 1110 7 0.3384786952526966 1110 8 0.47201704383726195 1110 9 0.7037594666242484 1110 10-11 -0.7643180046451974 1110 12-13 0.3851918217150043 1110 14-15 0.739446850679748 1110 16-17 0.35564633367831533 1110 18-19 -0.04314876056631789 1110 20-21 0.9169358685388929 1110 22-23 -0.2876084524593665 1110 24-25 -0.753885140686414 1110 26-27 1.0499552828579581 1110 28-29 0.15135213934403424 1110 30-31 0.16695129214922844 1110 32-33 0.5806837561489218 1110 34-35 0.5263878152029591 1110 36-37 1.1624082412587242 1110 38-39 0.9293648435240414 1110 40-41 -1.2870752621789876 1110 42-43 0.029057662743568358 1110 44-45 0.6960497589518297 1110 46-47 0.4064148803331378 1110 48-49 1.2208182913006738 1110 50-51 -0.27090748314851965 1110 52-53 0.16742004749514194 1110 54-55 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-0.924505041939895 2116 36-37 0.3699975269730089 2116 38-39 -3.0081351564759586 2116 40-41 -0.2803788336075641 2116 42-43 -1.0468351943992928 2116 44-45 0.14917475895183685 2116 46-47 -0.47081726252400813 2116 48-49 0.12037186272925027 2116 50-51 -0.8691217688628115 2116 52-53 -0.17409780964771215 2116 54-55 -0.5652016820522121 2116 56-57 -0.26306022361232095 2116 58-59 -1.7809565536141179 2116 60-61 -1.5102025102312027 2116 62-63 0.36448132570591696 2116 64-65 -1.78354040885214 2116 66-67 -0.3132397450300388 2116 68-69 -0.6812487491697397 2116 70-71 1.0534522919974023 2116 72-73 0.17900129494510253 2116 74-75 0.2965080179271169 2116 76 -0.9139040561740259 2117 1 0.7326837640858486 2117 2 0.44289910624785733 2117 3 0.28162331485611247 2117 4 0.20729817078492374 2117 5 0.08259534509899424 2117 6 0.47970691800924925 2117 7 0.7393952565674908 2117 8 0.5213612284137454 2117 9 -0.8622566522126718 2117 10-11 -0.27073788454406156 2117 12-13 -1.1304331782849957 2117 14-15 0.07924615535737445 2117 16-17 -0.815478034210436 2117 18-19 -0.6461828945738972 2117 20-21 0.011258719360633052 2117 22-23 0.01531111138387331 2117 24-25 -1.4900577070580923 2117 26-27 -1.6020872266237092 2117 28-29 -0.8745841754473673 2117 30-31 0.42836880163089575 2117 32-33 -0.9022689935350243 2117 34-35 0.5567291552788092 2117 36-37 -1.2298760507766744 2117 38-39 0.07583687260115823 2117 40-41 -0.2547388209653363 2117 42-43 0.3719187562960329 2117 44-45 -0.10609941930353983 2117 46-47 -0.9287818642939243 2117 48-49 -0.32581966697998155 2117 50-51 -0.2811990444633139 2117 52-53 0.7711060462309263 2117 54-55 -0.3391033887525978 2117 56-57 0.1481083920640387 2117 58-59 -0.49597314653446745 2117 60-61 -0.11633794006684894 2117 62-63 -0.19900634812467644 2117 64-65 -0.9145454657421581 2117 66-67 -0.13372014958122946 2117 68-69 0.3052398728277339 2117 70-71 0.2178802313147159 2117 72-73 1.14411665524851 2117 74-75 0.97209667153016 2117 76 0.9612144646856393 2118 1 5.337432873631087E-5 2118 2 0.11708475866736734 2118 3 -0.872961509094182 2118 4 -0.10780256354006212 2118 5 0.2461050419545785 2118 6 0.17889727764020336 2118 7 0.7505607621219994 2118 8 0.49897373076735363 2118 9 -0.8794662172663337 2118 10-11 -0.6004027310889697 2118 12-13 -0.9123480719020165 2118 14-15 0.6399597686128828 2118 16-17 -2.7994923441332347 2118 18-19 -0.5817505842745234 2118 20-21 -0.43096496732737677 2118 22-23 0.49549375118805017 2118 24-25 0.6713808167603972 2118 26-27 -1.7946780758046543 2118 28-29 -0.21668547463772825 2118 30-31 -0.7566999236562495 2118 32-33 0.08893793548023154 2118 34-35 -0.3248279902681688 2118 36-37 -2.4476316523582966 2118 38-39 0.30410838455747324 2118 40-41 -0.2181637880148557 2118 42-43 0.5351271916189404 2118 44-45 -2.0819137668840355 2118 46-47 0.2462040140717363 2118 48-49 0.31349496303320024 2118 50-51 -0.7317262521454779 2118 52-53 -0.8171259251492273 2118 54-55 -0.39269028387592897 2118 56-57 0.41282313505028867 2118 58-59 -0.6076187876566692 2118 60-61 -0.6535060816597777 2118 62-63 -0.7638811363001565 2118 64-65 0.4341647583211099 2118 66-67 0.029921349194886204 2118 68-69 -1.117608931540559 2118 70-71 0.20402220081199118 2118 72-73 1.5402284985925192 2118 74-75 0.5029194920912587 2118 76 0.4808717796922295 2119 1 0.6838426347137414 2119 2 -0.716856477401528 2119 3 0.015083070439764867 2119 4 -0.17588766992304272 2119 5 -0.4035403481163442 2119 6 -1.3588940090872512 2119 7 -0.619854638080632 2119 8 0.10505275812297299 2119 9 -0.15130005718527428 2119 10-11 -0.16595490940709823 2119 12-13 0.019566821715002902 2119 14-15 0.20581589829880187 2119 16-17 -0.30521676155977673 2119 18-19 -0.18124399866155017 2119 20-21 -1.393629607651583 2119 22-23 0.06611178563587572 2119 24-25 0.9848053355040634 2119 26-27 0.5667707590484383 2119 28-29 0.25626285362974954 2119 30-31 0.5833203397682709 2119 32-33 0.6379754228155861 2119 34-35 0.612102100917248 2119 36-37 0.3533308603063432 2119 38-39 -1.4121530136188198 2119 40-41 -0.5449621669408984 2119 42-43 0.08902790083880774 2119 44-45 0.4603354732375493 2119 46-47 0.3363255946188488 2119 48-49 -0.3802233753659934 2119 50-51 0.818527040661003 2119 52-53 0.286318857018955 2119 54-55 0.3577149846144536 2119 56-57 -0.5510066521837516 2119 58-59 -0.04449822028078643 2119 60-61 0.7703034421497534 2119 62-63 0.007040849515448144 2119 64-65 0.1295548292430979 2119 66-67 0.6629507311604428 2119 68-69 1.0729179174969303 2119 70-71 0.18380943485453827 2119 72-73 0.7037036758974793 2119 74-75 0.09993063697474014 2119 76 -0.5207492942692653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 3.0 21 0.0 22 8.0 23 12.0 24 23.0 25 68.0 26 97.0 27 189.0 28 374.0 29 660.0 30 1046.0 31 1814.0 32 2747.0 33 4329.0 34 6782.0 35 10753.0 36 18794.0 37 36760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.112952877101584 23.76983187307601 11.664693345962586 28.452521903859818 2 16.98555529244613 18.93085484252901 40.42742126450391 23.656168600520957 3 15.91404215013024 26.317783566185177 31.598389770305467 26.169784513379113 4 12.338385034335781 20.329149893440682 39.820033151787825 27.51243192043571 5 11.458678664456547 37.75988633672745 38.713000236798486 12.068434762017523 6 26.136632725550555 41.476438550793276 17.896045465309022 14.490883258347145 7 25.23087852237746 30.02486384087142 24.58086668245323 20.163390954297892 8 21.92280369405636 37.854605730523325 20.73170731707317 19.490883258347147 9 23.01562869997632 14.569026758228748 23.12573999526403 39.2896045465309 10-11 24.141013497513615 26.66646933459626 27.95583708264267 21.236680085247457 12-13 23.476201752308786 23.02273265451101 27.048898887047123 26.452166706133077 14-15 21.23312810798011 24.957376272791855 23.68458441865972 30.124911200568317 16-17 22.665166942931563 28.060028415818138 25.63639592706607 23.638408714184227 18-19 22.695950745915226 27.13710632251954 26.44387875917594 23.723064172389297 20-21 21.146696661141366 27.075538716552217 25.698555529244615 26.079209093061806 22-23 21.812100402557423 27.721406582997872 26.992067250769598 23.474425763675114 24-25 22.781790196542744 28.771607861709686 25.950745915226143 22.49585602652143 26-27 22.235377693582763 28.83495145631068 26.47051858868103 22.459152261425526 28-29 21.064409187781198 28.25953113900071 28.18671560502013 22.489344068197965 30-31 23.073644328676295 27.135922330097088 28.072460336253847 21.71797300497277 32-33 22.01337911437367 28.48922566895572 27.8321098744968 21.66528534217381 34-35 21.722708974662563 28.395690267582285 28.797655695003556 21.0839450627516 36-37 21.598981766516694 27.23478569737154 28.33767463888231 22.828557897229455 38-39 21.40835898650249 26.93997158418186 28.104428131659958 23.547241297655695 40-41 21.14847264977504 25.561212408240586 29.358276107032914 23.932038834951456 42-43 22.260241534454178 25.87733838503434 29.27835661851764 22.584063461993846 44-45 20.319085957849868 26.74046886099929 29.30262846317784 23.637816717973003 46-47 22.022851053753257 25.207198673928488 28.80712763438314 23.962822637935115 48-49 20.930618044044518 25.428013260715133 29.109045702107505 24.532322993132844 50-51 19.964480227326543 25.7275633435946 29.740113663272556 24.5678427658063 52-53 19.686834004262373 25.66244376035993 31.016457494672032 23.63426474070566 54-55 18.389770305470048 26.285223774567847 30.247454416291735 25.077551503670374 56-57 20.133199147525456 26.008761543926116 28.84205541084537 25.01598389770305 58-59 18.852711342647407 26.54688609992896 30.163982950509116 24.436419606914516 60-61 20.226734548898886 27.973004972768173 27.662206961875448 24.138053516457493 62-63 18.17191569973952 29.41747572815534 27.34548898887047 25.06511958323467 64-65 19.18896519062278 30.218446601941746 25.763083116268053 24.829505091167416 66-67 18.836135448733128 30.007695950745916 25.359933696424342 25.796234904096615 68-69 18.930262846317785 30.011247928013262 25.306654037414162 25.751835188254795 70-71 19.73419370116031 29.763793511721527 24.985792090930616 25.516220696187546 72-73 19.837201041913332 28.912502959981058 24.77859341700213 26.47170258110348 74-75 20.051503670376512 29.156405399005447 24.65841818612361 26.133672744494437 76 20.432157234193703 29.162917357328915 24.785697371536823 25.619228036940562 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 1.0 17 2.0 18 3.5 19 4.5 20 12.0 21 20.0 22 21.0 23 39.0 24 65.0 25 74.0 26 128.5 27 223.0 28 327.0 29 391.0 30 493.5 31 712.0 32 1045.5 33 1263.0 34 1413.5 35 1855.5 36 2356.5 37 2566.0 38 3192.0 39 4505.5 40 5599.0 41 6057.5 42 6110.0 43 6200.0 44 6315.5 45 6608.0 46 6875.0 47 6552.0 48 6023.5 49 5458.5 50 5099.0 51 4674.5 52 3734.5 53 2876.5 54 2534.0 55 2397.5 56 1890.0 57 1284.0 58 1049.0 59 866.0 60 563.5 61 361.5 62 279.0 63 230.0 64 150.0 65 95.5 66 51.0 67 30.0 68 27.5 69 20.0 70 12.5 71 10.0 72 6.0 73 2.5 74 2.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 84460.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.359696897939855 #Duplication Level Percentage of deduplicated Percentage of total 1 54.644908711719296 17.682926829268293 2 13.563352969155904 8.778119820033153 3 8.832461307672606 8.57447312337201 4 5.9273352603271015 7.672270897466256 5 3.7905674874684423 6.133080748283211 6 2.79536057956167 5.4274212645039075 7 1.8660129523251985 4.226852948141132 8 1.489151512934031 3.8550793274923043 9 1.233032088105082 3.5910490172862892 >10 5.784640152208116 29.760833530665405 >50 0.054882733891917605 1.0762491120056832 >100 0.010976546778383521 0.6334359460099456 >500 0.00365884892612784 0.7778830215486621 >1k 0.00365884892612784 1.8103244139237509 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 1529 1.8103244139237509 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 657 0.7778830215486621 Illumina PCR Primer Index 10 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 231 0.27350224958560265 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 187 0.2214065829978688 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 117 0.13852711342647409 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004735969689793985 0.0 2 0.0 0.0 0.0 0.00947193937958797 0.0 3 0.0 0.0 0.0 0.014207909069381956 0.0 4 0.0 0.0 0.0 0.020127871181624436 0.0 5 0.0 0.0 0.0 0.02249585602652143 0.0 6 0.0 0.0 0.0 0.02249585602652143 0.0 7 0.0 0.0 0.0 0.027231825716315414 0.0 8 0.0 0.0 0.0 0.030783802983660905 0.0 9 0.0 0.0 0.0 0.08406346199384324 0.0 10 0.0 0.0 0.0 0.11958323466729813 0.0 11 0.0 0.0 0.0 0.1349751361591286 0.0 12 0.0 0.0 0.0 0.13852711342647406 0.0 13 0.0 0.0 0.0 0.14207909069381955 0.0 14 0.0 0.0 0.0 0.14444707553871655 0.0 15 0.0 0.0 0.0 0.159838977030547 0.0 16 0.0 0.0 0.0 0.17286289367748045 0.0 17 0.0 0.0 0.0 0.17878285578972294 0.0 18 0.0 0.0 0.0 0.1941747572815534 0.0 19 0.0 0.0 0.0 0.21075065119583233 0.0 20 0.0 0.0 0.0 0.2285105375325598 0.0 21 0.0 0.0 0.0 0.23206251479990528 0.0 22 0.0 0.0 0.0 0.23561449206725077 0.0 23 0.0 0.0 0.0 0.24982240113663273 0.0 24 0.0 0.0 0.0 0.2545583708264267 0.0 25 0.0 0.0 0.0 0.2569263556713237 0.0 26 0.0 0.0 0.0 0.27823821927539666 0.0 27 0.0 0.0 0.0 0.319677954061094 0.0 28 0.0 0.0 0.0 0.3528297418896519 0.0 29 0.0 0.0 0.0 0.3800615676059673 0.0 30 0.0 0.0 0.0 0.4226852948141132 0.0 31 0.0 0.0 0.0 0.4499171205304286 0.0 32 0.0 0.0 0.0 0.47714894624674403 0.0 33 0.0 0.0 0.0 0.5126687189201989 0.0 34 0.0 0.0 0.0 0.5671323703528297 0.0 35 0.0 0.0 0.0 0.6784276580629884 0.0 36 0.0 0.0 0.0 0.8382666350935354 0.0 37 0.0 0.0 0.0 1.130712763438314 0.0 38 0.0 0.0 0.0 1.62206961875444 0.0 39 0.0 0.0 0.0 2.263793511721525 0.0 40 0.0 0.0 0.0 2.874733601704949 0.0 41 0.0 0.0 0.0 3.659720577788302 0.0 42 0.0 0.0 0.0 4.396163864551267 0.0 43 0.0 0.0 0.0 5.124319204357092 0.0 44 0.0 0.0 0.0 5.850106559318021 0.0 45 0.0 0.0 0.0 6.551030073407531 0.0 46 0.0 0.0 0.0 7.3265451101112955 0.0 47 0.0 0.0 0.0 8.126923987686478 0.0 48 0.0 0.0 0.0 8.901255031967795 0.0 49 0.0 0.0 0.0 9.667298129291973 0.0 50 0.0 0.0 0.0 10.344541794932512 0.0 51 0.0 0.0 0.0 11.071513142315888 0.0 52 0.0 0.0 0.0 11.843476201752308 0.0 53 0.0 0.0 0.0 12.555055647643854 0.0 54 0.0 0.0 0.0 13.299786881363959 0.0 55 0.0 0.0 0.0 13.96874260004736 0.0 56 0.0 0.0 0.0 14.55955481884916 0.0 57 0.0 0.0 0.0 15.209566658773383 0.0 58 0.0 0.0 0.0 15.828794695713947 0.0 59 0.0 0.0 0.0 16.360407293393322 0.0 60 0.0 0.0 0.0 16.892019891072696 0.0 61 0.0 0.0 0.0 17.49585602652143 0.0 62 0.0 0.0 0.0 18.11153208619465 0.0 63 0.0 0.0 0.0 18.701160312574 0.0 64 0.0 0.0 0.0 19.248164811745205 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTTAT 20 7.886222E-5 70.00001 1 TCATACC 15 0.002217754 70.0 23 CCACGAC 15 0.002217754 70.0 9 AATTTAC 35 2.9428338E-7 60.0 53 CGCCATT 35 2.9428338E-7 60.0 17 GAGGCGC 35 2.9428338E-7 60.0 13 GTCTCGA 25 2.3783301E-4 55.999996 60 TCTCGAG 25 2.3783301E-4 55.999996 61 ACGTCTC 25 2.3783301E-4 55.999996 58 CTCGAGT 25 2.3783301E-4 55.999996 62 GATGTTA 20 0.006929864 52.500004 38 ATGATTC 40 7.4013224E-7 52.500004 40 CGAGTGA 20 0.006929864 52.500004 64 ATGGCTC 20 0.006929864 52.500004 7 TGAAGGT 40 7.4013224E-7 52.500004 28 CGACCAA 20 0.006929864 52.500004 12 CGTTAAC 20 0.006929864 52.500004 1 CGTAGTG 20 0.006929864 52.500004 41 GAGCGTC 20 0.006929864 52.500004 28 AACTGTA 20 0.006929864 52.500004 7 >>END_MODULE