FastQCFastQC Report
Wed 25 May 2016
SRR1780697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524107
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT6130.11696084959750584No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT5830.11123682759436528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT250.002333899535.2541820
ACGTGAT353.2080273E-431.44085937
TTGCGAT502.3334323E-630.84150716
GTACATG502.3631928E-630.7914431
TACATGC502.3773118E-630.7679422
ATGTGAC300.005739154329.33638811
ATCTTAA300.005739154329.33638810
AGTTCCA300.005739154329.33638810
GTGACTT454.0702507E-529.32798625
ATAGAGC300.00577407129.3000056
CGGTAAC406.967768E-427.53969419
AGCGGTA406.975564E-427.53442617
CTACGTG407.0107356E-427.5107535
TACGTGA407.0107356E-427.5107536
AGAACGG508.353754E-526.39014627
TCGTTGT609.709709E-625.67669934
AGACTTC609.73133E-625.66933811
CGTTGAG609.752994E-625.66198530
GCGAACT609.76746E-625.65708528
CATCGTT609.789192E-625.6497432
Produced by FastQC (version 0.11.5)