FastQCFastQC Report
Wed 25 May 2016
SRR1780697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524107
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT147472.8137384160104713No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT50070.9553392723241629No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC27520.5250836184214293No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG12530.23907331899783824No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC12230.23334929699469764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT7250.035.5021444
TCGCATA250.00235090535.2020640
TGCAGAT250.00235310735.19534319
CGTTGTT451.0360254E-634.2209635
GCCGTCT7650.033.9333943
CCGAAAT401.819762E-532.99563624
GGTACAA551.3728095E-731.99576630
GTACAAT551.3728095E-731.99576631
TTGCGAT551.3728095E-731.99576616
CCCGCAG353.2178895E-431.42441630
ATGATTC1800.030.55734340
CCAACGT651.7451384E-830.4575122
AGCGGAT1900.030.10415335
CTCGCTA454.055644E-529.34344913
CTACCCG602.9311195E-729.34344910
TCGGAGC300.005740439529.3350537
TAAGATC300.005740439529.3350538
ATGCAGG454.0635747E-529.33504936
ATCGCAT454.0635747E-529.33504939
TGATTCG300.00574311729.33225343
Produced by FastQC (version 0.11.5)