FastQCFastQC Report
Wed 25 May 2016
SRR1780696_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780696_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73230
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAA150.002217512869.9924852
ATGTGTG150.002217512869.9924857
TACTGTG150.002217512869.99248539
CTCGCTT150.002217512869.99248545
GAGCTAT200.006929132652.4943611
GCGGTCA200.006929132652.4943626
TTATCGT200.006929132652.4943641
CAGTCAG200.006929132652.4943662
TATCGTG200.006929132652.4943642
TTACAAC200.006929132652.4943657
CTCCTTT305.846295E-446.66165526
CCCTACC400.002405981834.99624336
GAGAAAA400.002405981834.99624333
TATCAAT450.00428466131.10777520
TGGTCTG450.00428466131.1077755
AGAACAG500.007170794527.9969945
TGAGCTA500.007170794527.99699410
ATTTCCT657.8929286E-426.92018760
GTTGAAC800.002645242321.87265232
TAGGTCT800.002645242321.87265227
Produced by FastQC (version 0.11.5)