##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780696_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73230 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.257722244981565 34.0 31.0 34.0 12.0 34.0 2 31.308999044107605 34.0 32.0 34.0 23.0 34.0 3 31.45163184487232 34.0 33.0 34.0 23.0 34.0 4 31.911279530247167 34.0 34.0 34.0 23.0 34.0 5 31.816263826300695 34.0 34.0 34.0 23.0 34.0 6 34.37215622012836 38.0 35.0 38.0 24.0 38.0 7 33.81597705858255 37.0 34.0 38.0 22.0 38.0 8 32.44517274341117 37.0 31.0 38.0 11.0 38.0 9 33.34773999726888 37.0 34.0 38.0 21.0 38.0 10-11 33.90735354362966 37.0 34.0 38.0 22.5 38.0 12-13 34.3382834903728 38.0 34.5 38.0 24.5 38.0 14-15 33.89271473439847 37.0 34.0 38.0 22.0 38.0 16-17 33.7593609176567 37.0 34.0 38.0 21.5 38.0 18-19 33.74085074423051 37.0 34.0 38.0 22.0 38.0 20-21 33.81897446401748 37.0 34.0 38.0 22.0 38.0 22-23 33.8459715963403 37.0 34.0 38.0 22.0 38.0 24-25 34.2954526833265 38.0 34.5 38.0 23.5 38.0 26-27 34.34157449132869 38.0 34.5 38.0 24.0 38.0 28-29 34.510897173289635 38.0 35.5 38.0 24.5 38.0 30-31 34.32738631708316 38.0 35.0 38.0 24.0 38.0 32-33 34.07382903181757 38.0 34.0 38.0 23.0 38.0 34-35 33.90311347808275 37.0 34.0 38.0 23.0 38.0 36-37 33.88894578724566 37.0 34.0 38.0 22.5 38.0 38-39 34.4767786426328 38.0 35.0 38.0 25.0 38.0 40-41 33.91747234739861 37.5 34.0 38.0 22.5 38.0 42-43 34.30035504574628 38.0 34.5 38.0 24.0 38.0 44-45 33.30738768264372 37.0 34.0 38.0 21.5 38.0 46-47 34.05968182438891 37.0 34.0 38.0 24.0 38.0 48-49 33.77079748736857 37.0 34.0 38.0 22.5 38.0 50-51 33.71607947562474 37.0 34.0 38.0 22.0 38.0 52-53 33.75264918749147 37.0 34.0 38.0 22.5 38.0 54-55 33.46327324866858 37.0 34.0 38.0 21.5 38.0 56-57 33.58955346169603 37.0 34.0 38.0 22.0 38.0 58-59 33.43356547862898 37.0 33.0 38.0 22.0 38.0 60-61 33.471248122354226 37.0 33.5 38.0 21.5 38.0 62-63 33.54631981428376 37.0 33.5 38.0 22.5 38.0 64-65 33.5446128635805 37.0 33.5 38.0 22.0 38.0 66-67 33.44855250580363 37.0 33.5 38.0 21.5 38.0 68-69 33.042236788201556 37.0 32.0 38.0 20.5 38.0 70-71 32.8053871364195 37.0 31.5 38.0 19.5 38.0 72-73 32.898197460057354 37.0 32.0 38.0 19.5 38.0 74-75 32.46681005052574 37.0 31.0 38.0 15.0 38.0 76 31.6132322818517 36.0 28.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.2754950819429673 1101 2 -0.4752118141914714 1101 3 -0.18540823143671403 1101 4 -1.9670406855302645 1101 5 -0.7044115605942807 1101 6 -0.8032667669070008 1101 7 -1.0135215338026882 1101 8 -0.8411314566922883 1101 9 -0.17906851600466211 1101 10-11 -0.1154452463986928 1101 12-13 -0.9384537351900164 1101 14-15 0.18017794621108862 1101 16-17 -1.3960586690006664 1101 18-19 0.20624949103952872 1101 20-21 -1.0740062905629415 1101 22-23 -0.03965507499782461 1101 24-25 -1.305520379865392 1101 26-27 -0.9515767006670472 1101 28-29 0.09081257994470349 1101 30-31 -1.601336607049582 1101 32-33 0.22460261967165707 1101 34-35 -0.6606442087579012 1101 36-37 0.3509570665651509 1101 38-39 -0.9691566235633431 1101 40-41 0.45914726516646454 1101 42-43 -0.08488675979948823 1101 44-45 0.41884843939643446 1101 46-47 1.0742723045873745 1101 48-49 -0.6947426604899078 1101 50-51 -1.4025644969571331 1101 52-53 -0.045339326221444765 1101 54-55 -1.4434302874866987 1101 56-57 -0.9993692574845241 1101 58-59 0.291627757290172 1101 60-61 0.6511022001198938 1101 62-63 -1.1583858023485618 1101 64-65 -0.0443682567497774 1101 66-67 -0.4657986161163521 1101 68-69 0.7566608393459475 1101 70-71 0.3988409329885698 1101 72-73 -0.1767723104103638 1101 74-75 -0.229583062958163 1101 76 -2.117025240116206 1102 1 0.26845628583818737 1102 2 -0.19247625188144113 1102 3 0.32480453452073377 1102 4 0.5049957886338703 1102 5 0.49276169168535233 1102 6 1.793754509688739 1102 7 2.2534389525195024 1102 8 2.800965807745399 1102 9 1.9668889308038544 1102 10-11 0.18423560466514743 1102 12-13 -3.40032303610187 1102 14-15 -0.5910074641232512 1102 16-17 -1.6263170276632835 1102 18-19 0.20094553967174988 1102 20-21 0.7380149860328018 1102 22-23 -0.6000806069127194 1102 24-25 0.553127036547977 1102 26-27 -4.704023509177688 1102 28-29 0.33705878055260996 1102 30-31 0.6496968276008701 1102 32-33 -6.581400419842023 1102 34-35 -0.14730074371230728 1102 36-37 0.7599844221882464 1102 38-39 1.4191412487770876 1102 40-41 0.7996943776284695 1102 42-43 0.9386238784983902 1102 44-45 -5.064631803764666 1102 46-47 0.7759896298153421 1102 48-49 0.9134944215769636 1102 50-51 -5.645041700604551 1102 52-53 -0.45327245692053 1102 54-55 1.3387885574981055 1102 56-57 0.1155699522419198 1102 58-59 1.5961718302384966 1102 60-61 1.154430467597095 1102 62-63 -4.492884282591728 1102 64-65 -0.2181372537102675 1102 66-67 1.0251436331237684 1102 68-69 -7.102533689529434 1102 70-71 0.22650050745665595 1102 72-73 1.3076197868540689 1102 74-75 -6.036467561438407 1102 76 -1.1953535075934099 1103 1 -0.4080172965704456 1103 2 0.039784768173532825 1103 3 -0.5272264132548976 1103 4 0.16665719553603964 1103 5 0.21878734576252157 1103 6 -1.7251806425050873 1103 7 1.0300601476737299 1103 8 1.645539766821038 1103 9 0.32294105337333434 1103 10-11 0.30378920336207926 1103 12-13 -1.3726710967757967 1103 14-15 -0.5674912267212378 1103 16-17 -0.737726474879004 1103 18-19 -0.4319186566022992 1103 20-21 -0.6965900226203559 1103 22-23 -0.2740091419834698 1103 24-25 -0.03372270385719389 1103 26-27 -0.6801891408584311 1103 28-29 0.4288713632666443 1103 30-31 -0.31256012037835745 1103 32-33 -4.831514946972227 1103 34-35 -0.5440481732145059 1103 36-37 -0.3544154556494803 1103 38-39 0.13610653433139674 1103 40-41 0.38490256250069876 1103 42-43 0.46803190048759546 1103 44-45 -4.588109865456616 1103 46-47 -0.33394368994440526 1103 48-49 0.7860639013692889 1103 50-51 -0.5278549959318468 1103 52-53 -0.10386290790291497 1103 54-55 0.278852692691018 1103 56-57 0.67129376370481 1103 58-59 0.5042319951575678 1103 60-61 0.7248137517261881 1103 62-63 -0.09898730610796491 1103 64-65 0.38745676033839516 1103 66-67 0.5307427372671043 1103 68-69 -3.462136153135255 1103 70-71 -0.0016375359109517262 1103 72-73 0.9755038344973528 1103 74-75 -1.2456322769021142 1103 76 0.6236172752631539 1104 1 -2.1932037099486266 1104 2 0.27961138241390415 1104 3 -0.15233892450602227 1104 4 0.5504982112165564 1104 5 -0.29357704857165245 1104 6 -1.0113855787881931 1104 7 1.05315456237836 1104 8 1.5584442151606126 1104 9 -0.5317417833313911 1104 10-11 -0.41069277115116165 1104 12-13 -0.5553419954446142 1104 14-15 -0.11439065350602107 1104 16-17 -1.1280812998351806 1104 18-19 0.22893690263783384 1104 20-21 1.0133204421103272 1104 22-23 -0.08391250074039647 1104 24-25 0.258072265113384 1104 26-27 0.30664112111513475 1104 28-29 -0.13674046107367843 1104 30-31 -0.7764144005432172 1104 32-33 -0.7176180309648359 1104 34-35 -0.42178989475507933 1104 36-37 0.026784506451996037 1104 38-39 -0.16470117801878814 1104 40-41 0.6579732906261455 1104 42-43 0.6115488837648684 1104 44-45 -3.716964856218844 1104 46-47 -0.41205018480442135 1104 48-49 0.5038146238099515 1104 50-51 0.8598824620244798 1104 52-53 -0.14707907162455314 1104 54-55 -0.18770185750084067 1104 56-57 0.07809891790444112 1104 58-59 0.10041134993515044 1104 60-61 -0.1501566400498362 1104 62-63 0.31841759227661015 1104 64-65 -0.09332158065359408 1104 66-67 0.3540882297110812 1104 68-69 -3.6187281281222283 1104 70-71 -1.4481887699817264 1104 72-73 0.28068172070702957 1104 74-75 -1.2401488338209639 1104 76 0.0659167683703572 1105 1 -2.1010575388097728 1105 2 -0.971333277269455 1105 3 -1.6545869753980682 1105 4 -0.6635210167932044 1105 5 -0.4326931340932454 1105 6 0.1260085954118395 1105 7 0.5851534144374781 1105 8 0.029123891823928716 1105 9 0.8458590202272234 1105 10-11 0.44619726567860596 1105 12-13 -2.0464454536165135 1105 14-15 -0.6564818191443251 1105 16-17 0.3052663827591644 1105 18-19 0.38242616460750867 1105 20-21 -0.49072126640835734 1105 22-23 0.13889564963986345 1105 24-25 -0.1590607525361989 1105 26-27 0.22745711575807093 1105 28-29 -1.2315890763699926 1105 30-31 -0.46673343244641075 1105 32-33 0.41694216418511587 1105 34-35 0.8074993385160738 1105 36-37 0.20424899203098335 1105 38-39 0.0380897532482507 1105 40-41 0.2719422962223632 1105 42-43 0.407287153243999 1105 44-45 1.7103322213149923 1105 46-47 0.8778885916819235 1105 48-49 0.12489847132513887 1105 50-51 -0.9076714672814958 1105 52-53 -1.4047320108315162 1105 54-55 -1.1517808740981508 1105 56-57 -0.03412426093517951 1105 58-59 -0.31367244974916986 1105 60-61 -0.6188632933238551 1105 62-63 -0.0316087147019104 1105 64-65 -0.21656977503825203 1105 66-67 -0.19656466166500763 1105 68-69 0.34272019062269266 1105 70-71 0.9828989040030649 1105 72-73 -0.3276073690715151 1105 74-75 -1.434234546740079 1105 76 -1.2330500848367016 1106 1 -0.10308340759581114 1106 2 0.609857310232492 1106 3 -0.5335802744474663 1106 4 -0.3346290111831074 1106 5 0.44271593556547373 1106 6 -0.9738044013156042 1106 7 0.7069392956904039 1106 8 0.22952906558113284 1106 9 1.0503938495867402 1106 10-11 0.7796085170421776 1106 12-13 0.5726522555933968 1106 14-15 0.519379175089739 1106 16-17 0.7936955552696858 1106 18-19 -0.08757539375309875 1106 20-21 -0.3050815593801417 1106 22-23 -0.33223572015911174 1106 24-25 0.6215610333450527 1106 26-27 0.5773480305157506 1106 28-29 -0.5790645321904719 1106 30-31 -0.06261156864712092 1106 32-33 1.001561145017277 1106 34-35 1.2992789863265841 1106 36-37 1.2082228115728242 1106 38-39 0.5174025162216012 1106 40-41 0.32091377822330003 1106 42-43 0.9513497993403064 1106 44-45 1.5338868418541836 1106 46-47 0.9860388176442072 1106 48-49 0.8053955883580244 1106 50-51 1.2159454876818856 1106 52-53 0.5038311806910016 1106 54-55 0.561792446768294 1106 56-57 1.168618453728989 1106 58-59 0.04712698924101488 1106 60-61 1.5883986670937844 1106 62-63 0.9058231070216394 1106 64-65 1.027859085800145 1106 66-67 1.374262827816061 1106 68-69 1.41265162275608 1106 70-71 0.7224968469670685 1106 72-73 1.1674058769936266 1106 74-75 -1.1312113271670725 1106 76 0.46610840043679147 1107 1 -0.7545427009905872 1107 2 -0.40473562371527905 1107 3 0.026020339991855934 1107 4 0.10914979066021147 1107 5 -0.2625067986895182 1107 6 0.4767332330929932 1107 7 -0.39828343856459014 1107 8 -0.18398859954942992 1107 9 -1.4324018493379924 1107 10-11 -0.6759214368748729 1107 12-13 -0.11797754471382405 1107 14-15 -1.1760125299793884 1107 16-17 0.13632228338028085 1107 18-19 0.8357733005633463 1107 20-21 0.6650413284846763 1107 22-23 -0.7406074559502045 1107 24-25 -0.880282284627306 1107 26-27 -0.2382433673337161 1107 28-29 -1.4610921819600549 1107 30-31 -0.6675270832400528 1107 32-33 0.23174547681450974 1107 34-35 -0.3815965897102842 1107 36-37 -0.5595191239110449 1107 38-39 -0.07629948070620429 1107 40-41 -0.08085273483354172 1107 42-43 0.11463704972432254 1107 44-45 -0.36496108441308905 1107 46-47 -0.6157276954126303 1107 48-49 -0.08093313668038604 1107 50-51 0.43791169351905523 1107 52-53 0.26227972139761135 1107 54-55 0.6446649506085436 1107 56-57 0.136821218705947 1107 58-59 -0.4259912903288807 1107 60-61 -1.011754942737248 1107 62-63 -1.4417191356819004 1107 64-65 -0.521987304368821 1107 66-67 0.371344241026506 1107 68-69 0.6423751250602336 1107 70-71 0.18217426632190126 1107 72-73 -0.2739151675532199 1107 74-75 -0.4419640153391171 1107 76 -0.7970252401162057 1108 1 -0.8403240557860272 1108 2 -0.3564458852846961 1108 3 -0.1623096398874182 1108 4 -0.3418227110165155 1108 5 -0.18162148681829393 1108 6 -0.013407611977427791 1108 7 0.9558413099263561 1108 8 1.012657946392892 1108 9 -0.29437368032390765 1108 10-11 -0.01760487081183726 1108 12-13 0.19982929633245305 1108 14-15 0.6852886101949949 1108 16-17 -0.4151867709456738 1108 18-19 -0.5945419241180829 1108 20-21 0.0018622540380022201 1108 22-23 -0.3793733848569758 1108 24-25 -0.614051566988131 1108 26-27 -0.38096637202855277 1108 28-29 -0.024505998190775813 1108 30-31 0.5927344861764396 1108 32-33 0.47664822664684436 1108 34-35 -0.20855836033403108 1108 36-37 0.2005747728025753 1108 38-39 0.7303738928685775 1108 40-41 0.5622056152670609 1108 42-43 0.25746065804089113 1108 44-45 -0.13296913270283284 1108 46-47 0.4937625795706069 1108 48-49 0.3246671315959446 1108 50-51 -0.007930023449425505 1108 52-53 -0.11297178765671845 1108 54-55 0.17219680842209328 1108 56-57 0.9484912388266693 1108 58-59 -0.3121415250706647 1108 60-61 0.9045428439830729 1108 62-63 -0.0398076668690166 1108 64-65 0.8549073971737613 1108 66-67 0.4044763671163736 1108 68-69 1.2903965871661995 1108 70-71 1.4227845949604045 1108 72-73 0.8510345306359071 1108 74-75 0.9237636841913996 1108 76 -0.35450344266483214 1109 1 0.14572184927396137 1109 2 -1.3280160469957032 1109 3 0.13607325004476678 1109 4 0.10862069013111153 1109 5 0.21183182564910652 1109 6 0.5378443442041103 1109 7 -0.857278147559299 1109 8 1.0232865327256988 1109 9 1.0203759284397833 1109 10-11 1.4164066054531617 1109 12-13 0.4810303917941141 1109 14-15 0.24951657054971577 1109 16-17 -1.0694316848736847 1109 18-19 0.45237382966386974 1109 20-21 1.1742344501777993 1109 22-23 1.0088634435206956 1109 24-25 0.7674690381240268 1109 26-27 0.5126825585922106 1109 28-29 0.16152686565899188 1109 30-31 0.3179887897758178 1109 32-33 0.9902110852801229 1109 34-35 -0.18616008177377807 1109 36-37 0.3770549501630285 1109 38-39 0.567880413473695 1109 40-41 0.1411049371241333 1109 42-43 0.3268724994597747 1109 44-45 -0.21297695742897105 1109 46-47 -0.6220107641956929 1109 48-49 0.25835257760532926 1109 50-51 1.0748164554238144 1109 52-53 0.6842373933552821 1109 54-55 0.33785278129636964 1109 56-57 0.003818573203304254 1109 58-59 0.22215685781927164 1109 60-61 0.09399902551672312 1109 62-63 -0.06804188700464664 1109 64-65 0.14262909774757304 1109 66-67 0.024651119333384486 1109 68-69 1.4319915271766348 1109 70-71 0.45995204409968693 1109 72-73 -0.41637548501353905 1109 74-75 1.5918322809571848 1109 76 -0.5353850284759929 1110 1 0.9250960510619564 1110 2 0.050108382852865674 1110 3 -0.030137295476123427 1110 4 -0.19068600572730787 1110 5 -0.05002732414108024 1110 6 0.6373243660979213 1110 7 0.5655917666899271 1110 8 2.6169966221254413 1110 9 -0.6044872352164816 1110 10-11 -1.0068738178272554 1110 12-13 -0.4880103854363185 1110 14-15 -0.03923092078397872 1110 16-17 0.16226645415203933 1110 18-19 0.5149194417784457 1110 20-21 0.140082379387799 1110 22-23 -0.18330039519486974 1110 24-25 0.11979981717893651 1110 26-27 -1.0981776858887216 1110 28-29 0.3271672597476609 1110 30-31 0.07541216142332274 1110 32-33 1.4802676443022023 1110 34-35 0.8195528355770705 1110 36-37 0.02209007149125597 1110 38-39 0.7919271202790199 1110 40-41 0.9851571173832099 1110 42-43 1.0161969840428782 1110 44-45 1.6770750403816521 1110 46-47 0.6441245213361384 1110 48-49 1.201907585815512 1110 50-51 0.5565488035354704 1110 52-53 0.5348577181135354 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2116 44-45 1.2036897092377004 2116 46-47 0.18863738395245377 2116 48-49 0.1444636887164421 2116 50-51 0.033149788757150134 2116 52-53 1.0499781340960155 2116 54-55 -0.3172398112962256 2116 56-57 -0.25960735272261815 2116 58-59 -1.2009912903288793 2116 60-61 -2.6740565300388326 2116 62-63 0.40788403892127434 2116 64-65 0.7458698384883178 2116 66-67 -0.5123859177036536 2116 68-69 -0.32032328763818185 2116 70-71 -3.556714622566986 2116 72-73 0.061402292764235256 2116 74-75 -1.20029734867245 2116 76 -1.4835331766241424 2117 1 0.26053666408877874 2117 2 0.49050247152281656 2117 3 0.7879251018966187 2117 4 0.1512132827237025 2117 5 -1.391871878054598 2117 6 -1.6510445446847797 2117 7 -2.394315184596337 2117 8 -2.4943060598668936 2117 9 0.49537592843978473 2117 10-11 -0.15766746862090741 2117 12-13 0.990752614016337 2117 14-15 -0.6208537998206509 2117 16-17 -0.27915390709591037 2117 18-19 0.29450345929349453 2117 20-21 -0.37345073500738124 2117 22-23 0.5570115916688394 2117 24-25 0.5498764455314316 2117 26-27 1.0589788548885082 2117 28-29 0.6004157545478748 2117 30-31 0.11937767866470494 2117 32-33 1.128174048243082 2117 34-35 0.1957843626706648 2117 36-37 -0.798408012799932 2117 38-39 0.4252878208811026 2117 40-41 -1.8987098776906777 2117 42-43 -0.6448867597994905 2117 44-45 0.5564674870154747 2117 46-47 -0.028029282714214787 2117 48-49 -1.033314089061335 2117 50-51 0.6164831220904858 2117 52-53 0.9860892452071326 2117 54-55 -1.158906477962887 2117 56-57 -0.648496241611511 2117 58-59 -0.6426579569955422 2117 60-61 0.5481656921833888 2117 62-63 -0.4837826277453914 2117 64-65 -1.2624634948450222 2117 66-67 -2.301274806592545 2117 68-69 -0.5703232876381819 2117 70-71 0.7210631552107927 2117 72-73 1.0475134038753495 2117 74-75 0.013591540216438602 2117 76 -0.9224220655130324 2118 1 -0.34766439411228234 2118 2 -0.15076736974702598 2118 3 -0.38773627376475517 2118 4 -0.15857507706465768 2118 5 -0.45086658704930827 2118 6 0.13175968811945182 2118 7 0.45885941857826396 2118 8 -4.185046800607633 2118 9 -3.0715552884914317 2118 10-11 -1.4116357225891605 2118 12-13 0.15265737592109474 2118 14-15 -2.0421500961169485 2118 16-17 -1.092249145191147 2118 18-19 -1.0446499798599405 2118 20-21 -4.46035549691215 2118 22-23 -1.291401106743855 2118 24-25 -0.48583784018286025 2118 26-27 -0.048825377915726165 2118 28-29 -0.8576530285209003 2118 30-31 -1.2127651784781577 2118 32-33 -0.6500005549315233 2118 34-35 0.2374510293373362 2118 36-37 -0.3574027217946494 2118 38-39 -0.3606910150977356 2118 40-41 -2.243815697796503 2118 42-43 -0.8718708867836114 2118 44-45 -0.5205166399686476 2118 46-47 -0.5403308700158007 2118 48-49 -1.2886050943523415 2118 50-51 -0.2829877773804199 2118 52-53 -2.187455728337845 2118 54-55 -0.3873456314020416 2118 56-57 0.9690963509810828 2118 58-59 0.7023155879779992 2118 60-61 -2.0524956834779893 2118 62-63 0.5331485891858208 2118 64-65 -0.622251854633376 2118 66-67 -2.4244229547406917 2118 68-69 0.8132745959597045 2118 70-71 -0.8217939876463518 2118 72-73 -2.1273543209923815 2118 74-75 0.3262899529148555 2118 76 -0.46792471101567656 2119 1 0.7713684455909515 2119 2 0.5508376670535426 2119 3 0.21883757861915143 2119 4 0.5426161380930381 2119 5 0.9403131002197682 2119 6 0.9057835124226017 2119 7 -1.4807521802512085 2119 8 -0.18670208717912118 2119 9 0.1255124895819293 2119 10-11 -0.13376926874505557 2119 12-13 0.35599159601509456 2119 14-15 -0.5245626762948916 2119 16-17 -0.9584369610996362 2119 18-19 -1.319959607124872 2119 20-21 -1.6143414860936645 2119 22-23 0.6369929696949157 2119 24-25 -0.020731872283590747 2119 26-27 0.40775290827150457 2119 28-29 0.9443480947092766 2119 30-31 0.2812491870445868 2119 32-33 0.09842544489113436 2119 34-35 0.3082145302684296 2119 36-37 -0.3563533883430736 2119 38-39 0.07274281777743852 2119 40-41 -0.7309724475230865 2119 42-43 -1.0381828491849632 2119 44-45 -1.1346870753681344 2119 46-47 -0.7236686371524499 2119 48-49 -0.7805207929719487 2119 50-51 1.3222559340085454 2119 52-53 -1.9150280732286262 2119 54-55 -2.082137390439609 2119 56-57 0.38177998433510396 2119 58-59 1.3193532161888157 2119 60-61 1.2800247859139944 2119 62-63 0.10051284090761925 2119 64-65 0.45448250143059 2119 66-67 0.8503546161262676 2119 68-69 -0.2996839331999439 2119 70-71 -1.032443983212545 2119 72-73 -2.0700843614877797 2119 74-75 0.1001216457409626 2119 76 0.9814264757656765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 11.0 13 51.0 14 183.0 15 319.0 16 511.0 17 602.0 18 675.0 19 683.0 20 602.0 21 548.0 22 587.0 23 652.0 24 735.0 25 883.0 26 1083.0 27 1208.0 28 1498.0 29 1804.0 30 2143.0 31 2706.0 32 3462.0 33 4499.0 34 6350.0 35 9097.0 36 14995.0 37 17342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.05242462156105 20.946475465057127 11.247989220848858 28.753110692532964 2 17.995357094087122 19.863443943738904 34.35340707360371 27.787791888570258 3 17.899767854704358 23.0547589785607 29.24074832718831 29.80472483954663 4 13.741635941554009 18.628977195138603 36.38672675133142 31.24266011197597 5 13.758022668305339 35.361190768810594 35.50594018844736 15.374846374436707 6 28.745049842960533 35.85962037416359 17.055851427010786 18.339478355865083 7 27.30301788884337 30.581728799672263 21.513041103372935 20.60221220811143 8 23.120305885566026 33.349720060084664 19.203878191997813 24.326095862351497 9 22.75433565478629 16.758159224361602 21.315034821794345 39.172470299057764 10-11 24.327461422914105 25.15499112385634 26.911102007374026 23.606445445855524 12-13 23.77304383449406 23.56479584869589 26.366926123173563 26.295234193636485 14-15 21.81551276799126 25.136556056261096 23.63990167963949 29.408029496108153 16-17 22.018298511538987 27.616414037962585 26.106786835996175 24.258500614502253 18-19 21.736310255359825 26.79434657927079 26.719240748327188 24.750102417042196 20-21 21.77537740937737 27.176889095241673 26.115158508524573 24.93257498685639 22-23 22.592516728116895 27.18831080158405 26.172333743001502 24.046838727297555 24-25 21.92885429468797 27.707223815376214 26.614092585006144 23.749829304929673 26-27 20.992079748736856 27.951659156083576 26.96231052847194 24.093950566707633 28-29 21.95411716509627 26.78683599617643 28.07455960671856 23.184487232008742 30-31 22.255906049433293 26.751331421548546 27.459374573262323 23.53338795575584 32-33 20.89512494879148 27.68332650553052 27.270927215622017 24.150621330055987 34-35 22.04356138194729 27.113887750921755 27.04083026082207 23.80172060630889 36-37 22.35149528881606 27.378123719786974 26.337566571077424 23.93281442031954 38-39 21.58473303291001 27.341253584596476 27.854704356138193 23.21930902635532 40-41 21.97255223269152 25.809777413628293 27.94141745186399 24.276252901816196 42-43 21.393554554144476 25.303837225180935 28.31831216714461 24.984296053529974 44-45 20.55236924757613 25.176840092858114 28.905503209067323 25.365287450498432 46-47 20.714870954526834 24.99385497746825 29.878465109927628 24.41280895807729 48-49 19.526150484774 25.621330055987983 29.814966543766214 25.0375529154718 50-51 19.5514133551823 26.00573535436296 29.396422231325957 25.046429059128773 52-53 18.839956302061996 26.102690154308345 29.69070053256862 25.36665301106104 54-55 18.0547589785607 26.378533387955756 29.548682234057082 26.018025399426463 56-57 18.506759524784925 26.685784514543222 29.005871910419227 25.80158405025263 58-59 18.702717465519598 25.996859210705995 28.743001502116616 26.55742182165779 60-61 18.551823023351087 25.742864946060358 29.005871910419227 26.69944012016933 62-63 18.88638536119077 25.672538577085895 28.592789840229415 26.84828622149392 64-65 20.073057490099686 25.577632117984432 27.467567936637987 26.88174245527789 66-67 19.904410760617232 25.537348081387414 26.79707770039601 27.76116345759935 68-69 20.026628430970913 25.277208794210022 26.58473303291001 28.111429741909056 70-71 21.09859347262051 25.046429059128773 26.35395329782876 27.501024170421957 72-73 21.87150075105831 24.81769766489144 26.063088897992625 27.24771268605763 74-75 22.252492148026764 25.854840912194454 25.454731667349446 26.437935272429332 76 23.09982247712686 25.60835723064318 25.171377850607673 26.120442441622288 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 3.5 16 5.5 17 5.0 18 5.0 19 8.5 20 14.5 21 17.0 22 29.5 23 59.0 24 74.5 25 73.0 26 131.0 27 208.5 28 285.0 29 342.0 30 420.0 31 612.5 32 810.0 33 893.0 34 1052.5 35 1429.5 36 1864.0 37 2081.0 38 2335.0 39 3045.0 40 3735.5 41 4193.5 42 4417.0 43 4451.5 44 4797.0 45 5120.0 46 5132.0 47 5222.5 48 5071.5 49 4620.5 50 4411.0 51 4327.0 52 3863.5 53 3246.5 54 3009.0 55 2730.0 56 2215.0 57 1767.5 58 1556.0 59 1359.5 60 1031.5 61 775.5 62 651.0 63 579.0 64 469.5 65 375.0 66 261.0 67 204.0 68 191.0 69 146.5 70 102.5 71 90.0 72 64.0 73 31.0 74 19.5 75 15.0 76 13.5 77 9.0 78 9.0 79 12.0 80 9.0 81 5.5 82 3.0 83 1.0 84 2.0 85 1.5 86 0.0 87 0.0 88 1.0 89 2.0 90 1.5 91 2.0 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 1.0 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.678683599617643 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.827802813054759E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 73230.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.30452000546225 #Duplication Level Percentage of deduplicated Percentage of total 1 74.5771155544485 44.973371569029084 2 11.716220194289079 14.130820701898127 3 5.724508050089446 10.356411306841458 4 2.8214940784855416 6.805953844052984 5 1.827404270736623 5.51003687013519 6 0.8921899413509657 3.22818517001229 7 0.6363080546183284 2.6860576266557423 8 0.4642105024795634 2.2395193226819607 9 0.3011707162428387 1.6345759934453092 >10 1.03937863725912 8.43506759524785 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.019117847876553327 0.0 2 0.0 0.0 0.0 0.019117847876553327 0.0 3 0.0 0.0 0.0 0.02184896900177523 0.0 4 0.0 0.0 0.0 0.027311211252219036 0.0 5 0.0 0.0 0.0 0.03277345350266284 0.0 6 0.0 0.0 0.0 0.03277345350266284 0.0 7 0.0 0.0 0.0 0.038235695753106654 0.0 8 0.0 0.0 0.0 0.0396012563157176 0.0 9 0.0 0.0 0.0 0.06554690700532569 0.0 10 0.0 0.0 0.0 0.0710091492557695 0.0 11 0.0 0.0 0.0 0.0819336337566571 0.0 12 0.0 0.0 0.0 0.08329919431926806 0.0 13 0.0 0.0 0.0 0.09149255769493377 0.0 14 0.0 0.0 0.0 0.09832036050798854 0.0 15 0.0 0.0 0.0 0.10241704219582139 0.0 16 0.0 0.0 0.0 0.10924484500887614 0.0 17 0.0 0.0 0.0 0.1160726478219309 0.0 18 0.0 0.0 0.0 0.12016932950976376 0.0 19 0.0 0.0 0.0 0.12426601119759662 0.0 20 0.0 0.0 0.0 0.12426601119759662 0.0 21 0.0 0.0 0.0 0.12972825344804043 0.0 22 0.0 0.0 0.0 0.13792161682370613 0.0 23 0.0 0.0 0.0 0.1474805407619828 0.0 24 0.0 0.0 0.0 0.15157722244981564 0.0 25 0.0 0.0 0.0 0.15703946470025945 0.0 26 0.0 0.0 0.0 0.16250170695070326 0.0 27 0.0 0.0 0.0 0.20756520551686466 0.0 28 0.0 0.0 0.0 0.24443534070736037 0.0 29 0.0 0.0 0.0 0.2717465519595794 0.0 30 0.0 0.0 0.0 0.340024580090127 0.0 31 0.0 0.0 0.0 0.3878191997815103 0.0 32 0.0 0.0 0.0 0.46565615185033454 0.0 33 0.0 0.0 0.0 0.5339341799808821 0.0 34 0.0 0.0 0.0 0.6158678137375393 0.0 35 0.0 0.0 0.0 0.7401338249351359 0.0 36 0.0 0.0 0.0 0.9695479994537758 0.0 37 0.0 0.0 0.0 1.418817424552779 0.0 38 0.0 0.0 0.0 2.012836269288543 0.0 39 0.0 0.0 0.0 2.684692066093131 0.0 40 0.0 0.0 0.0 3.6050798852929127 0.0 41 0.0 0.0 0.0 4.681141608630343 0.0 42 0.0 0.0 0.0 5.940188447357641 0.0 43 0.0 0.0 0.0 7.017615731257681 0.0 44 0.0 0.0 0.0 8.209750102417042 0.0 45 0.0 0.0 0.0 9.483818107333061 0.0 46 0.0 0.0 0.0 10.654103509490646 0.0 47 0.0 0.0 0.0 11.661887204697528 0.0 48 0.0 0.0 0.0 12.643725249214803 0.0 49 0.0 0.0 0.0 13.714324730301788 0.0 50 0.0 0.0 0.0 14.611498019937184 0.0 51 0.0 0.0 0.0 15.60972279120579 0.0 52 0.0 0.0 0.0 16.57244298784651 0.0 53 0.0 0.0 0.0 17.64713915062133 0.0 54 0.0 0.0 0.0 18.7901133415267 0.0 55 0.0 0.0 0.0 19.878465109927625 0.0 56 0.0 0.0 0.0 20.84528198825618 0.0 57 0.0 0.0 0.0 21.675542810323638 0.0 58 0.0 0.0 0.0 22.505803632391096 0.0 59 0.0 0.0 0.0 23.35518230233511 0.0 60 0.0 0.0 0.0 24.15949747371296 0.0 61 0.0 0.0 0.0 24.90782466202376 0.0 62 0.0 0.0 0.0 25.736719923528607 0.0 63 0.0 0.0 0.0 26.509627201966406 0.0 64 0.0 0.0 0.0 27.2442987846511 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTCAA 15 0.0022175128 69.992485 2 ATGTGTG 15 0.0022175128 69.992485 7 TACTGTG 15 0.0022175128 69.992485 39 CTCGCTT 15 0.0022175128 69.992485 45 GAGCTAT 20 0.0069291326 52.49436 11 GCGGTCA 20 0.0069291326 52.49436 26 TTATCGT 20 0.0069291326 52.49436 41 CAGTCAG 20 0.0069291326 52.49436 62 TATCGTG 20 0.0069291326 52.49436 42 TTACAAC 20 0.0069291326 52.49436 57 CTCCTTT 30 5.846295E-4 46.661655 26 CCCTACC 40 0.0024059818 34.996243 36 GAGAAAA 40 0.0024059818 34.996243 33 TATCAAT 45 0.004284661 31.107775 20 TGGTCTG 45 0.004284661 31.107775 5 AGAACAG 50 0.0071707945 27.996994 5 TGAGCTA 50 0.0071707945 27.996994 10 ATTTCCT 65 7.8929286E-4 26.920187 60 GTTGAAC 80 0.0026452423 21.872652 32 TAGGTCT 80 0.0026452423 21.872652 27 >>END_MODULE