FastQCFastQC Report
Wed 25 May 2016
SRR1780696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780696_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73230
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT9011.2303700669124675No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4400.6008466475488188RNA PCR Primer, Index 24 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1530.2089307660794756No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1030.14065273794892805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTC150.00221656970.0000134
GATACTA150.00221656970.0000152
GGAAAGT150.00221656970.0000119
TTGGCTC200.006926191552.526
AGATCAA200.006926191552.531
GGTCGTA200.006926191552.511
GAATAGA200.006926191552.55
ATTCAGC200.006926191552.543
ATTCAAC200.006926191552.559
GTATGTA200.006926191552.522
GTCGTAA200.006926191552.512
ACGTCTC200.006926191552.558
TTACGTC200.006926191552.556
GTCCACA200.006926191552.511
ACCTACA305.843202E-446.6666687
TGCCTAC305.843202E-446.66666845
TCTATAC305.843202E-446.6666683
CCCTAAG400.00240471535.01
ATGTGTA400.00240471535.020
CGTATGC2100.033.33333241
Produced by FastQC (version 0.11.5)