FastQCFastQC Report
Wed 25 May 2016
SRR1780695_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780695_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415989
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCAT301.3051147E-436.64060641
TTAGACC250.002342576435.22582636
AAACGAT250.002352284335.19614825
CTCGTAA401.8155803E-533.0043331
CGTTGAG401.8215718E-532.9884530
CCGAATC551.3612589E-732.0234834
AGATCAA502.351906E-630.80775322
AACGCTG300.005720187429.35485334
CGAATCA602.9186958E-729.35485335
GCGCGCT300.00577427529.2983888
ACCCGGT300.00577427529.2983888
CCCGTGT508.355199E-526.38758533
GCCGTTG508.355199E-526.38758528
AGAACGG450.001391691424.45943324
TCGTAAG551.6040841E-423.9714092
CGTAAGG551.6053929E-423.9685273
CACGTAC651.7994305E-523.7096939
TCCCGAA752.067285E-623.45563132
CATGTAC752.0824773E-623.4358943
TAAGATG500.002559484422.02675219
Produced by FastQC (version 0.11.5)