FastQCFastQC Report
Wed 25 May 2016
SRR1780695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415989
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT147743.5515362185057775No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT65751.5805706400890407No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC27930.671411984451512No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG17410.4185206820372654No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT5610.13485933522280638No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC4830.11610883941642687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT301.3007755E-436.66135825
CCGTCTT8600.036.06888244
GGGCTAA250.002351307835.19913544
CGCGTGT250.002351307835.19913542
ACGAGTG250.002352695235.1949045
CACTTGC250.002352695235.19490432
CGAAGGA250.002352695235.1949044
GCCGTCT9200.033.47743643
GAGTCCC353.2147256E-431.4277979
TATCTTG353.2169934E-431.4240229
TTTTCGA353.2169934E-431.4240221
ATCTACG300.00572121229.3537851
CCGCTTT300.005731312629.34319512
CTTATAT300.005731312629.34319512
ATCGTAT300.00573805429.33613839
TCGAGAT300.005741426729.3326119
TGCCGTC10500.029.33261142
CGGGTTT300.005744801429.32908631
GTCTAGA300.005744801429.32908632
CGTCCGC300.005744801429.32908624