FastQCFastQC Report
Wed 25 May 2016
SRR1780694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131241
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG200.00673437952.8974651
TAACGGT200.00694137652.49428636
AAGCCAG200.00694137652.49428612
GTTGTTC200.00694137652.4942868
CCTGTAC305.86342E-446.66158740
TTAACGG305.86342E-446.66158735
TGGTACA305.86342E-446.66158746
CTGCTAC404.5754172E-543.74523567
GTTCTTA350.001206101440.302831
GCCACTA350.001252378539.99564440
TATGTCA350.001252378539.99564452
GTCGCCC400.002412945334.9961937
AAGAGCG400.002412945334.9961927
CGGGGCG400.002412945334.9961951
CGCCATT400.002412945334.9961933
AGAAGTC652.091491E-532.3041735
GGAAGAG552.981029E-431.8147186
CGATGGT552.981029E-431.81471832
ATGTAGA450.004296986431.10772361
TAGCAAC450.004296986431.10772366