##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780694_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 131241 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.06975716430079 34.0 31.0 34.0 12.0 34.0 2 31.355856782560327 34.0 32.0 34.0 23.0 34.0 3 31.60397284385215 34.0 33.0 34.0 23.0 34.0 4 31.983473152444738 34.0 34.0 34.0 27.0 34.0 5 31.981133944422854 34.0 34.0 34.0 27.0 34.0 6 34.92078694920033 38.0 36.0 38.0 27.0 38.0 7 34.16394267035454 38.0 34.0 38.0 24.0 38.0 8 33.288530261122666 37.0 34.0 38.0 21.0 38.0 9 33.80343795003086 37.0 34.0 38.0 21.0 38.0 10-11 34.20898194923842 37.5 34.5 38.0 24.0 38.0 12-13 34.58333142844081 38.0 35.5 38.0 25.0 38.0 14-15 34.129890811560415 38.0 34.0 38.0 23.5 38.0 16-17 34.00349357289262 37.5 34.0 38.0 23.0 38.0 18-19 33.97383439626336 37.5 34.0 38.0 22.5 38.0 20-21 34.05680008533919 38.0 34.0 38.0 23.0 38.0 22-23 34.04895192813221 37.5 34.0 38.0 23.0 38.0 24-25 34.45758947280194 38.0 35.0 38.0 25.0 38.0 26-27 34.54962245030136 38.0 35.5 38.0 25.0 38.0 28-29 34.67816840773844 38.0 36.0 38.0 26.0 38.0 30-31 34.52847814326316 38.0 35.5 38.0 25.0 38.0 32-33 34.238915430391415 38.0 34.0 38.0 23.5 38.0 34-35 34.10025449364147 37.0 34.0 38.0 23.5 38.0 36-37 34.08628401185605 37.5 34.0 38.0 23.5 38.0 38-39 34.650589373747536 38.0 36.0 38.0 26.0 38.0 40-41 34.160601488864 37.5 34.5 38.0 23.5 38.0 42-43 34.47712605054823 38.0 35.0 38.0 24.5 38.0 44-45 33.55873164636051 37.0 34.0 38.0 22.0 38.0 46-47 34.29015703933984 37.0 34.5 38.0 24.0 38.0 48-49 33.98356839707104 37.0 34.0 38.0 23.5 38.0 50-51 33.94549340526208 37.0 34.0 38.0 23.0 38.0 52-53 33.955829352107955 37.0 34.0 38.0 23.0 38.0 54-55 33.645122332198014 37.0 34.0 38.0 22.5 38.0 56-57 33.76511151240847 37.0 34.0 38.0 22.5 38.0 58-59 33.650193156102134 37.0 33.5 38.0 22.5 38.0 60-61 33.666647617741404 37.0 34.0 38.0 22.5 38.0 62-63 33.772178663679796 37.0 34.0 38.0 23.0 38.0 64-65 33.78044208745743 37.0 34.0 38.0 23.0 38.0 66-67 33.670068804718035 37.0 34.0 38.0 22.5 38.0 68-69 33.251499150417935 37.0 32.5 38.0 21.0 38.0 70-71 33.10151172270861 37.0 32.5 38.0 20.5 38.0 72-73 33.182694432380124 37.0 32.0 38.0 21.0 38.0 74-75 32.793041046624154 37.0 31.5 38.0 19.5 38.0 76 32.11363064895878 36.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.099152829821726 1101 2 -0.8040816025785382 1101 3 -0.15829271553040059 1101 4 -2.7031036461230684 1101 5 -1.0508548270457077 1101 6 -2.0055844649947474 1101 7 -0.8725567946012518 1101 8 -0.30055783931235425 1101 9 -0.9581448763058447 1101 10-11 -1.0844551167799281 1101 12-13 -0.9599355755805021 1101 14-15 -0.6065731736383242 1101 16-17 -1.5170200165258194 1101 18-19 0.445484000033872 1101 20-21 -0.9040807807492399 1101 22-23 -0.13851321376873216 1101 24-25 -1.27280674470488 1101 26-27 -0.7864167150799304 1101 28-29 -0.4567957120336956 1101 30-31 -0.8704458297072151 1101 32-33 0.0345906835208325 1101 34-35 -1.1793018283896828 1101 36-37 -0.16753623103222992 1101 38-39 -0.7714992296805718 1101 40-41 0.30622861493752396 1101 42-43 -0.5496109487620942 1101 44-45 -0.39875054090068573 1101 46-47 0.5671466541413253 1101 48-49 -1.261099909556556 1101 50-51 -1.3231782765048692 1101 52-53 -0.05430332940279925 1101 54-55 -1.5098161884048977 1101 56-57 -0.7468227535979111 1101 58-59 -0.35025404658760806 1101 60-61 -0.195150505491668 1101 62-63 -1.7744058533900429 1101 64-65 -0.22947506761325087 1101 66-67 -1.141260927703847 1101 68-69 0.31462331720024395 1101 70-71 0.4407773880257011 1101 72-73 -1.0107882402312853 1101 74-75 -1.5859682430374633 1101 76 -2.1452989800033713 1102 1 0.4698986860383023 1102 2 -0.39167220346701015 1102 3 0.2972154288075295 1102 4 0.3459864481797936 1102 5 0.2952015987979486 1102 6 0.5207385028175864 1102 7 1.3376762347807158 1102 8 2.1018659780050797 1102 9 1.0097356474257495 1102 10-11 -0.23067813135401138 1102 12-13 -4.046084355878218 1102 14-15 -1.0804353044153459 1102 16-17 -2.3147559685170904 1102 18-19 -1.0969961262228551 1102 20-21 0.11087323686435724 1102 22-23 -1.0191782048060674 1102 24-25 0.3367326520685623 1102 26-27 -5.8635541166854175 1102 28-29 0.1532746254302566 1102 30-31 0.40242845912452907 1102 32-33 -6.591037677479868 1102 34-35 -0.3432504684021609 1102 36-37 0.5887132818662195 1102 38-39 0.5883665251319954 1102 40-41 0.341952642682827 1102 42-43 0.5471761294079585 1102 44-45 -5.779430144984545 1102 46-47 -0.06289971792631377 1102 48-49 0.38229304500661243 1102 50-51 -5.870948325604701 1102 52-53 -1.708276129652198 1102 54-55 0.5899957903964435 1102 56-57 -0.5178363145050895 1102 58-59 0.9511692641442124 1102 60-61 0.036402958774559124 1102 62-63 -4.6496250490879625 1102 64-65 -0.6251379154037622 1102 66-67 0.03417356687958062 1102 68-69 -8.051029204037384 1102 70-71 -0.5237957161750657 1102 72-73 0.25122368401468265 1102 74-75 -6.510633117170421 1102 76 -1.61316976170394 1103 1 0.03503971466105682 1103 2 -0.06719810349187227 1103 3 -0.13753126501961077 1103 4 0.3704789937524495 1103 5 0.25600871398669867 1103 6 -1.260320274479895 1103 7 0.22462878147614873 1103 8 1.1254282238768738 1103 9 0.5234615779259428 1103 10-11 0.5479065299286816 1103 12-13 -0.9341676978996958 1103 14-15 -0.12804499557188365 1103 16-17 -0.32269960518694063 1103 18-19 -0.005918983526100874 1103 20-21 -0.3914886016003294 1103 22-23 -0.3247616069762316 1103 24-25 0.6777717005965229 1103 26-27 -0.6051874342464316 1103 28-29 0.45369326029565116 1103 30-31 0.3492449895878238 1103 32-33 -5.602802760765755 1103 34-35 -0.8809035468119859 1103 36-37 0.021274573378839534 1103 38-39 0.7756093894926863 1103 40-41 0.83904946603014 1103 42-43 1.0974443925544648 1103 44-45 -5.073874010219406 1103 46-47 0.08606452167397549 1103 48-49 0.5539413596403833 1103 50-51 0.5349630874082649 1103 52-53 -0.1790157981126299 1103 54-55 0.4874142087265625 1103 56-57 1.1828758459555644 1103 58-59 0.7775240130835925 1103 60-61 1.0599531749088484 1103 62-63 0.6401592978175898 1103 64-65 1.2818401343351908 1103 66-67 0.18290523155466332 1103 68-69 -3.0196275792208347 1103 70-71 0.5546862913475294 1103 72-73 1.5338383073636024 1103 74-75 -1.0719045802270983 1103 76 0.7100051266199969 1104 1 -1.6632553939242882 1104 2 -0.06094973444666962 1104 3 0.09491241267472716 1104 4 -0.5680303860498057 1104 5 -0.32374859993948135 1104 6 -0.619137578547857 1104 7 0.5816556596112079 1104 8 0.922885384130872 1104 9 -0.06940861256957476 1104 10-11 -0.4564789263037383 1104 12-13 -0.8683604840054073 1104 14-15 -0.633129950195098 1104 16-17 -0.7912874157932137 1104 18-19 0.5752916923415654 1104 20-21 0.7983001716317162 1104 22-23 0.29944466168913664 1104 24-25 0.0019185300203972133 1104 26-27 0.4365686329054199 1104 28-29 -0.8347719025098854 1104 30-31 -0.8919659762273611 1104 32-33 -1.0622499758198245 1104 34-35 -0.303386077473931 1104 36-37 -0.07252707352307652 1104 38-39 -0.682442453123798 1104 40-41 0.45961689332580846 1104 42-43 0.28051725636611025 1104 44-45 -3.5727432148933573 1104 46-47 -0.26023429823962374 1104 48-49 0.38404661058996936 1104 50-51 0.7722887564621672 1104 52-53 -0.48928501438448535 1104 54-55 -0.6556495217382263 1104 56-57 0.03614427936912534 1104 58-59 0.7232349644013993 1104 60-61 0.18145205861089408 1104 62-63 0.09853370704951914 1104 64-65 -0.19925528739347698 1104 66-67 -0.37431953576246 1104 68-69 -3.7291037890268584 1104 70-71 -0.9110999013515908 1104 72-73 0.3724535180104809 1104 74-75 -1.5964993785685948 1104 76 0.023199188494800183 1105 1 -0.11590220947433139 1105 2 0.22042211950583024 1105 3 -0.35235805878929227 1105 4 0.10303779308289052 1105 5 0.31349587601467377 1105 6 0.3147215730532267 1105 7 1.5145399795922998 1105 8 0.6630484468745763 1105 9 0.6013050823376673 1105 10-11 1.0709936011687873 1105 12-13 -0.8759818948526288 1105 14-15 0.08190904306970026 1105 16-17 1.2919129859555767 1105 18-19 1.3142288304722456 1105 20-21 -0.21611552020333136 1105 22-23 0.4716501443785006 1105 24-25 0.49756132808255416 1105 26-27 0.5713169491053449 1105 28-29 -0.5182617997094638 1105 30-31 0.3846203406815434 1105 32-33 0.8711808406755068 1105 34-35 0.49150048757392284 1105 36-37 0.7206461511017608 1105 38-39 0.5329362541903961 1105 40-41 0.38666699376300073 1105 42-43 0.7374031124455129 1105 44-45 1.209706861911556 1105 46-47 1.0331309386739989 1105 48-49 0.3847851193929941 1105 50-51 0.1511290022379015 1105 52-53 -0.8882570101306726 1105 54-55 -0.7742392653279779 1105 56-57 -0.3061693210089942 1105 58-59 0.11536836863157873 1105 60-61 0.2844297261046904 1105 62-63 0.6139833112087985 1105 64-65 0.33679871237020365 1105 66-67 0.871559585116664 1105 68-69 1.215005864760215 1105 70-71 1.514753814824708 1105 72-73 0.6079048945908809 1105 74-75 -0.8019525275701334 1105 76 -0.8649019576708632 1106 1 -0.30499120912948996 1106 2 0.15221465218710506 1106 3 -1.1495930818307691 1106 4 -0.058330901860525586 1106 5 0.501268219975298 1106 6 0.20946748985312524 1106 7 1.2348290168661933 1106 8 1.6772024955908407 1106 9 1.5061688711711696 1106 10-11 0.6212851463582965 1106 12-13 0.49991155008295607 1106 14-15 0.29019927160350534 1106 16-17 0.5150312655254652 1106 18-19 0.2982957291211079 1106 20-21 0.049617731622745964 1106 22-23 0.039689111117262144 1106 24-25 0.6351322550708574 1106 26-27 0.20145425748491874 1106 28-29 -0.9186191776836949 1106 30-31 -0.5686031145991919 1106 32-33 1.2724943614755233 1106 34-35 1.3576365733446352 1106 36-37 0.49315469368930565 1106 38-39 0.18798813669454972 1106 40-41 0.5738241146758796 1106 42-43 0.5415623337377156 1106 44-45 0.9534958521620354 1106 46-47 0.9282608800514751 1106 48-49 0.6600053457323796 1106 50-51 0.8295044999024057 1106 52-53 0.38235100265969635 1106 54-55 0.5644390631462741 1106 56-57 1.0802965556642548 1106 58-59 0.04362630775192855 1106 60-61 1.1918523725826375 1106 62-63 1.0414300963744765 1106 64-65 1.117744039809935 1106 66-67 1.3730173110641815 1106 68-69 1.7824178525968222 1106 70-71 0.7409521282514575 1106 72-73 0.6239673103405963 1106 74-75 -1.2940212444578094 1106 76 0.38757460899266505 1107 1 -0.42941742338419076 1107 2 -0.10747767459163171 1107 3 0.5175801704488663 1107 4 0.4318527095779672 1107 5 -0.20251876594369023 1107 6 0.1377597794133294 1107 7 0.6948190919235699 1107 8 0.9761060995856283 1107 9 -0.8386686078934034 1107 10-11 -0.6643787392567404 1107 12-13 -0.06052204584781862 1107 14-15 -0.7548274016797905 1107 16-17 0.38870147829142354 1107 18-19 1.4385281898865685 1107 20-21 0.4098853949190655 1107 22-23 -0.4523089038942132 1107 24-25 -0.7286168920043821 1107 26-27 0.08800363407446099 1107 28-29 -0.9694457393113822 1107 30-31 -0.26341500592106826 1107 32-33 0.971272352915264 1107 34-35 0.6149562185279365 1107 36-37 0.11986829706378899 1107 38-39 0.6185716923052453 1107 40-41 0.434467840251223 1107 42-43 0.32757126618607657 1107 44-45 0.20347259057776768 1107 46-47 0.3573130480311306 1107 48-49 0.22822769546254307 1107 50-51 0.7952750786506186 1107 52-53 0.6433576089496285 1107 54-55 0.6663635715514573 1107 56-57 0.8180299468930841 1107 58-59 0.6249914525940596 1107 60-61 -0.38380980718288527 1107 62-63 -0.357261827203466 1107 64-65 0.37220251012815453 1107 66-67 1.055070223414539 1107 68-69 0.9299738354762965 1107 70-71 0.1012042838249343 1107 72-73 0.6041872098505507 1107 74-75 -0.16336867948044898 1107 76 -0.4995679379957316 1108 1 -0.8210803276112912 1108 2 -0.28054048852018454 1108 3 -0.5251141125242107 1108 4 -0.5772194728250994 1108 5 0.08464473086764102 1108 6 -0.26531037127237767 1108 7 0.8201929843554225 1108 8 1.2383457857981668 1108 9 -0.016168748984888737 1108 10-11 0.23213418472316505 1108 12-13 0.2881855561613236 1108 14-15 0.8119723612865215 1108 16-17 -1.15340374773713 1108 18-19 -0.5463926577910598 1108 20-21 -0.0686475358332359 1108 22-23 -0.5320255921949126 1108 24-25 -0.8775812805138941 1108 26-27 -0.5846041598191007 1108 28-29 0.6069200262510108 1108 30-31 0.20802455048730195 1108 32-33 -0.030561836373067308 1108 34-35 -0.3509419698574163 1108 36-37 -0.20538990035142035 1108 38-39 0.5544844131134141 1108 40-41 0.25925690848306004 1108 42-43 0.6810477603448888 1108 44-45 0.41115220003653974 1108 46-47 0.33317229516697466 1108 48-49 0.06991246886962443 1108 50-51 0.23633763861450063 1108 52-53 -0.3514960791817572 1108 54-55 0.04494729523789687 1108 56-57 0.7157104029007542 1108 58-59 -1.1130192985770009 1108 60-61 0.7069825625896726 1108 62-63 -0.03849736532638559 1108 64-65 0.1292782480366128 1108 66-67 0.3463606461246229 1108 68-69 1.1285600988094444 1108 70-71 1.3499838424907793 1108 72-73 0.7362276748880774 1108 74-75 1.0273164607644816 1108 76 0.27357811990820835 1109 1 -0.18686141753031293 1109 2 -0.36274053623747093 1109 3 -0.019963452201139376 1109 4 -0.31904788706730614 1109 5 0.16129413510325108 1109 6 0.5639108545005769 1109 7 -1.2614456834901375 1109 8 0.935705896951383 1109 9 0.8495983064373434 1109 10-11 0.8831629133380972 1109 12-13 0.11123862059369571 1109 14-15 0.16942397735882508 1109 16-17 -0.6397306392364399 1109 18-19 0.18553792758779508 1109 20-21 0.6603779087094495 1109 22-23 0.7034943157387943 1109 24-25 0.6435546716565383 1109 26-27 0.5757118970486772 1109 28-29 0.21462777188979487 1109 30-31 0.27617606691467955 1109 32-33 0.8697300811602275 1109 34-35 0.3152545411666807 1109 36-37 0.23959136359536615 1109 38-39 0.19708542640407956 1109 40-41 -0.12931269560378666 1109 42-43 0.3527598286086828 1109 44-45 0.0661456739955284 1109 46-47 -0.6538036918090171 1109 48-49 -0.5267185501751968 1109 50-51 0.9343257934992053 1109 52-53 0.25944310934363557 1109 54-55 -0.15122339231210447 1109 56-57 0.08879995702479704 1109 58-59 -0.013791897625459626 1109 60-61 0.20337920553804167 1109 62-63 -0.3563401228243137 1109 64-65 -0.3504559385941235 1109 66-67 -0.04907533551826049 1109 68-69 0.9226564877334198 1109 70-71 0.1873941971425097 1109 72-73 -0.6072982117412522 1109 74-75 0.9881567060874872 1109 76 -0.5155676002719893 1110 1 0.4477585920896985 1110 2 0.8779551107081716 1110 3 0.3280854895978038 1110 4 0.38095812543870267 1110 5 0.6348240773831968 1110 6 1.0325856981754171 1110 7 1.341458356913904 1110 8 2.9370587108041946 1110 9 -0.3613354357463976 1110 10-11 -0.7301427624675725 1110 12-13 0.12383482068989338 1110 14-15 0.20141050934535798 1110 16-17 0.4780994473436522 1110 18-19 1.137573160873032 1110 20-21 0.5896182285747713 1110 22-23 0.5314314249259056 1110 24-25 0.8035700035468665 1110 26-27 -0.8629755728887858 1110 28-29 0.7622092413462553 1110 30-31 0.7121954873091028 1110 32-33 1.5851807184858728 1110 34-35 1.2184982881604327 1110 36-37 0.35122105335005216 1110 38-39 1.189201527895186 1110 40-41 1.0773897454736527 1110 42-43 1.1009936549675103 1110 44-45 1.8381739928988523 1110 46-47 0.6213060364257146 1110 48-49 1.3203759062942666 1110 50-51 1.2988715486699576 1110 52-53 0.8320967288722585 1110 54-55 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0.09057757645284426 2116 48-49 0.44189473261719314 2116 50-51 -0.04951294929475836 2116 52-53 0.7193380789400976 2116 54-55 0.07600949089093234 2116 56-57 -0.5101694814968809 2116 58-59 -0.5438677090445552 2116 60-61 -1.5789669999424945 2116 62-63 0.678134330306861 2116 64-65 0.6763228657966138 2116 66-67 0.0820073463106965 2116 68-69 0.6208757090869668 2116 70-71 -2.9551707558702063 2116 72-73 0.6461290961636692 2116 74-75 -1.7586528966922614 2116 76 -0.48619067537267924 2117 1 -0.3377943053053052 2117 2 0.19754907668372113 2117 3 0.49201092819429704 2117 4 0.037955733094207744 2117 5 -1.017903859884214 2117 6 -1.1442484326060836 2117 7 -2.0012117653614823 2117 8 -2.1683041281112736 2117 9 0.11512322985700507 2117 10-11 -0.13368476140431085 2117 12-13 0.9022439115989869 2117 14-15 -0.5099132546099838 2117 16-17 -0.05660616137511454 2117 18-19 0.4713218317927499 2117 20-21 0.13162547206228226 2117 22-23 0.22695574709496213 2117 24-25 0.8632706282146003 2117 26-27 0.7136957455588444 2117 28-29 0.7151847198151557 2117 30-31 -0.15789522241976073 2117 32-33 0.9259818216225 2117 34-35 0.29297662415192605 2117 36-37 -0.8021797308073104 2117 38-39 0.6521118814305353 2117 40-41 -1.239936477280203 2117 42-43 -0.9322582721988937 2117 44-45 0.6945917892837556 2117 46-47 -0.12532972912453033 2117 48-49 -0.5955016189582594 2117 50-51 0.6606555066710271 2117 52-53 1.1493066571019241 2117 54-55 -0.4730696746846945 2117 56-57 -0.7535141616051106 2117 58-59 -0.8170500429888676 2117 60-61 0.8112878660782812 2117 62-63 -0.27662695100588053 2117 64-65 -0.37106301183278134 2117 66-67 -1.6050486020178383 2117 68-69 0.010445404469699326 2117 70-71 0.9685832809631734 2117 72-73 1.2226238155491913 2117 74-75 0.1942274384740088 2117 76 -0.5849076169804306 2118 1 0.6279533972845037 2118 2 0.5964129029159686 2118 3 0.7275497753120916 2118 4 0.3687828007633769 2118 5 0.19526238907150528 2118 6 0.9545986851884081 2118 7 1.4179193958749394 2118 8 -3.513817912572428 2118 9 -0.49622179938276645 2118 10-11 -0.2318635416883552 2118 12-13 0.452243911598984 2118 14-15 -0.5741739062390607 2118 16-17 -0.18211159161738522 2118 18-19 -0.5280599560100825 2118 20-21 -2.552743784412243 2118 22-23 -0.1597861075416276 2118 24-25 -0.09390564580377969 2118 26-27 0.700579621916404 2118 28-29 -0.3638378365758186 2118 30-31 -0.26927366853504964 2118 32-33 0.288354419784568 2118 34-35 1.0199106258227673 2118 36-37 0.46752787153187114 2118 38-39 0.3633817227003746 2118 40-41 -0.9500534363445254 2118 42-43 0.06106670691556104 2118 44-45 0.738685356535207 2118 46-47 -0.22946506747039308 2118 48-49 -0.7423270157836583 2118 50-51 0.06728875646216892 2118 52-53 -0.8879113880108633 2118 54-55 0.5638559727672643 2118 56-57 1.26070471892956 2118 58-59 0.6749382611046997 2118 60-61 -1.2079435457847083 2118 62-63 0.46836887188467813 2118 64-65 0.20404141590322666 2118 66-67 -1.714154700597625 2118 68-69 1.3955665406434719 2118 70-71 -0.5660449562966434 2118 72-73 -1.436557471000512 2118 74-75 0.9520720500028261 2118 76 -0.28921839392278414 2119 1 0.51247007820837 2119 2 0.41131452823675474 2119 3 0.3559672261247506 2119 4 -0.012312152187860192 2119 5 0.08207162773703303 2119 6 0.3958818331781373 2119 7 -1.8476066948008523 2119 8 0.5769947478210753 2119 9 -1.013706829322885 2119 10-11 0.3030295293125036 2119 12-13 0.3169145702816252 2119 14-15 -0.6910772424238303 2119 16-17 -1.0418549620194426 2119 18-19 -1.3047750971563659 2119 20-21 -1.3338558460033454 2119 22-23 0.4615654750075606 2119 24-25 -0.16813147984997556 2119 26-27 0.0028892882979292267 2119 28-29 0.5494897938775622 2119 30-31 -0.12800762918518416 2119 32-33 -0.191773895014407 2119 34-35 0.18990207150283567 2119 36-37 -0.8512745236777093 2119 38-39 -0.26987747319355293 2119 40-41 -0.4359216998175768 2119 42-43 -0.9327485197739236 2119 44-45 -1.2408356015486177 2119 46-47 -0.14644539253454525 2119 48-49 -0.8533193168957212 2119 50-51 0.4471176700635411 2119 52-53 -1.4898361099954656 2119 54-55 -1.004279185487654 2119 56-57 0.9516456941220355 2119 58-59 1.2856568239789539 2119 60-61 0.8374285671323136 2119 62-63 -0.40611359175945694 2119 64-65 0.060713785449848956 2119 66-67 0.4673679683501959 2119 68-69 -0.7838432112812512 2119 70-71 -0.719680542267561 2119 72-73 -2.540210165611292 2119 74-75 -0.03405284306816725 2119 76 0.012424035504075448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 23.0 13 137.0 14 392.0 15 569.0 16 765.0 17 960.0 18 964.0 19 1033.0 20 968.0 21 1012.0 22 997.0 23 1048.0 24 1219.0 25 1426.0 26 1734.0 27 2044.0 28 2473.0 29 2943.0 30 3766.0 31 4711.0 32 6003.0 33 7941.0 34 10878.0 35 16134.0 36 27079.0 37 34019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.226094768607034 23.31974230801717 13.086544884936998 28.367618038438795 2 17.100601184081192 20.258912992128984 38.27462454568313 24.36586127810669 3 16.807247735082786 25.4562217599683 29.39096776159889 28.345562743350023 4 12.337607912161594 19.702684374547587 39.28497954145428 28.674728171836545 5 11.995489214498518 38.37977461311633 35.8005501329615 13.824186039423655 6 27.49750459079099 39.02439024390244 17.985995230149115 15.492109935157458 7 25.722144756592837 30.103397566309308 24.696550620613987 19.477907056483872 8 22.067036977773714 36.71261267439291 20.072233524584544 21.148116823248834 9 23.091869156742177 16.450651854222382 23.053009349212516 37.40446963982292 10-11 24.663024512157026 26.418954442590348 27.095953246317844 21.822067798934782 12-13 23.477038425492033 24.08317522725368 26.623920878384045 25.815865468870246 14-15 21.443375164773204 25.468794050639666 24.133845368444312 28.953985416142817 16-17 22.01065215900519 28.16193110384712 25.945017182130588 23.882399555017106 18-19 21.347368581464636 26.813267195464828 27.322635456907523 24.516728766163013 20-21 21.219740782225067 27.821336320204814 26.544296370798758 24.41462652677136 22-23 22.422108944613345 26.945847715271903 26.922608026455148 23.7094353136596 24-25 21.55271599576352 27.897912999748552 26.65173230926311 23.897638695224817 26-27 21.047919476383143 27.859053192218898 27.410641491607045 23.68238583979092 28-29 21.55423990978429 27.30320555314269 28.775306497207424 22.367248039865594 30-31 21.759587324083174 27.275013143758432 27.41254638413301 23.552853148025388 32-33 20.74808939279646 28.37680298077582 27.29063326247133 23.58447436395639 34-35 22.025891299212898 27.463978482334028 27.45902576176652 23.051104456686552 36-37 22.325340404294387 27.33520774757888 26.716879633651068 23.62257221447566 38-39 21.593861674324337 27.451025213157475 27.943630420371683 23.011482692146508 40-41 21.55043012473236 26.332853300416794 28.187075685189843 23.929640889661005 42-43 21.396133830129305 26.201415716125297 28.198886018850818 24.203564434894584 44-45 20.425019620393016 26.316090246188313 28.549386243628135 24.709503889790536 46-47 20.60255560381283 26.04483355049108 29.38411014850542 23.968500697190663 48-49 20.121379751754407 26.480672960431573 29.814615859373216 23.583331428440808 50-51 19.733924611973393 26.37018919392568 29.349822083038074 24.546064111062854 52-53 19.432570614365936 26.716498655145877 29.191335024877894 24.65959570561029 54-55 18.940727364162115 27.550460603012777 28.793974443961872 24.714837588863237 56-57 19.17007642428814 26.721451375713386 28.983320761042663 25.12515143895581 58-59 19.3925678713207 26.68449646070969 28.36651656113562 25.556419106833992 60-61 19.646680534284254 26.61134858771268 28.20802950297544 25.53394137502762 62-63 19.384186344206462 26.252847814326312 28.680061870909245 25.68290397055798 64-65 20.464641384933064 26.12445805807636 27.41597519067974 25.994925366310834 66-67 20.514930547618505 26.265420104997677 26.868128100212584 26.351521247171235 68-69 20.38349296332701 26.08521727204151 27.031186900435078 26.5001028641964 70-71 21.346225645949055 26.33932993500507 26.503912649248328 25.810531769797546 72-73 21.8053047447063 25.820818189437748 25.921777493313826 26.452099572542114 74-75 21.91312166167585 26.44867076599538 26.102742283280378 25.535465289048393 76 22.716224350622138 25.70690561638512 26.058167798172825 25.518702234819912 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 1.0 11 1.5 12 1.0 13 1.0 14 1.5 15 3.0 16 6.5 17 9.0 18 10.0 19 14.0 20 16.0 21 15.0 22 26.5 23 55.0 24 98.5 25 125.0 26 213.5 27 360.0 28 536.5 29 655.0 30 779.0 31 1088.5 32 1589.0 33 1904.0 34 2257.0 35 2946.5 36 3625.5 37 3968.0 38 4574.0 39 5814.0 40 7196.5 41 8231.0 42 8517.0 43 8866.5 44 9678.5 45 10090.5 46 10040.0 47 10112.5 48 9699.5 49 8708.5 50 8203.0 51 7677.0 52 6419.0 53 5148.0 54 4609.0 55 4320.0 56 3399.5 57 2392.5 58 2017.0 59 1666.5 60 1113.5 61 751.5 62 592.0 63 513.0 64 364.0 65 253.5 66 188.0 67 163.0 68 137.0 69 87.0 70 59.0 71 55.0 72 39.0 73 23.0 74 19.0 75 15.0 76 13.0 77 7.5 78 5.0 79 6.0 80 6.5 81 6.0 82 5.0 83 5.0 84 4.5 85 4.0 86 2.5 87 1.0 88 1.5 89 1.5 90 1.5 91 2.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 3.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7680526664685579 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 131241.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.20247483636973 #Duplication Level Percentage of deduplicated Percentage of total 1 74.40313806518265 35.12012252268727 2 8.661963873508855 8.177322635456907 3 4.908876656604626 6.95133380574668 4 2.8765597507627243 5.431229570027659 5 1.925777655813653 4.545073566949353 6 1.3607967844517264 3.8539785585297275 7 1.0524786517942177 3.4775717953993035 8 0.7570743676249819 2.8588627029662987 9 0.6505351175967328 2.7636180766681147 >10 3.380199841805356 26.198367888083755 >50 0.02259923485447707 0.6225188774849323 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0038097850519273704 0.0 2 0.0 0.0 0.0 0.0038097850519273704 0.0 3 0.0 0.0 0.0 0.0038097850519273704 0.0 4 0.0 0.0 0.0 0.010667398145396637 0.0 5 0.0 0.0 0.0 0.011429355155782112 0.0 6 0.0 0.0 0.0 0.012953269176553059 0.0 7 0.0 0.0 0.0 0.016001097218094954 0.0 8 0.0 0.0 0.0 0.01676305422848043 0.0 9 0.0 0.0 0.0 0.031240237425804437 0.0 10 0.0 0.0 0.0 0.038859807529659174 0.0 11 0.0 0.0 0.0 0.04647937763351392 0.0 12 0.0 0.0 0.0 0.05714677577891055 0.0 13 0.0 0.0 0.0 0.0617185178412234 0.0 14 0.0 0.0 0.0 0.06705221691392171 0.0 15 0.0 0.0 0.0 0.07238591598662003 0.0 16 0.0 0.0 0.0 0.07390983000739099 0.0 17 0.0 0.0 0.0 0.0822913571216312 0.0 18 0.0 0.0 0.0 0.08762505619432952 0.0 19 0.0 0.0 0.0 0.09676854031895521 0.0 20 0.0 0.0 0.0 0.10286419640203899 0.0 21 0.0 0.0 0.0 0.10819789547473732 0.0 22 0.0 0.0 0.0 0.1142935515578211 0.0 23 0.0 0.0 0.0 0.11810333660974848 0.0 24 0.0 0.0 0.0 0.1287707347551451 0.0 25 0.0 0.0 0.0 0.13791421887977082 0.0 26 0.0 0.0 0.0 0.1493435740355529 0.0 27 0.0 0.0 0.0 0.16763054228480428 0.0 28 0.0 0.0 0.0 0.18058381146135735 0.0 29 0.0 0.0 0.0 0.19810882270022326 0.0 30 0.0 0.0 0.0 0.21944361899101653 0.0 31 0.0 0.0 0.0 0.2552555984791338 0.0 32 0.0 0.0 0.0 0.3024969331230332 0.0 33 0.0 0.0 0.0 0.35659588086040184 0.0 34 0.0 0.0 0.0 0.4076470005562286 0.0 35 0.0 0.0 0.0 0.541751434384072 0.0 36 0.0 0.0 0.0 0.704048277596178 0.0 37 0.0 0.0 0.0 1.031689792061932 0.0 38 0.0 0.0 0.0 1.4431465776700878 0.0 39 0.0 0.0 0.0 1.9925175821580146 0.0 40 0.0 0.0 0.0 2.671421278411472 0.0 41 0.0 0.0 0.0 3.433378288796946 0.0 42 0.0 0.0 0.0 4.351536486311442 0.0 43 0.0 0.0 0.0 5.2224533491820395 0.0 44 0.0 0.0 0.0 6.017174511014089 0.0 45 0.0 0.0 0.0 6.805800016763055 0.0 46 0.0 0.0 0.0 7.7475788815995 0.0 47 0.0 0.0 0.0 8.572778323846968 0.0 48 0.0 0.0 0.0 9.365975571658247 0.0 49 0.0 0.0 0.0 10.054784709046716 0.0 50 0.0 0.0 0.0 10.79312105211024 0.0 51 0.0 0.0 0.0 11.58784221394229 0.0 52 0.0 0.0 0.0 12.418375355262457 0.0 53 0.0 0.0 0.0 13.186428021731015 0.0 54 0.0 0.0 0.0 13.946099161085332 0.0 55 0.0 0.0 0.0 14.698150730335794 0.0 56 0.0 0.0 0.0 15.272666316166442 0.0 57 0.0 0.0 0.0 15.908900419838313 0.0 58 0.0 0.0 0.0 16.52227581319862 0.0 59 0.0 0.0 0.0 17.14479469068355 0.0 60 0.0 0.0 0.0 17.7193102765142 0.0 61 0.0 0.0 0.0 18.367735692352237 0.0 62 0.0 0.0 0.0 19.012351323138347 0.0 63 0.0 0.0 0.0 19.598296264124777 0.0 64 0.0 0.0 0.0 20.190336861194293 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 20 0.006734379 52.897465 1 TAACGGT 20 0.006941376 52.494286 36 AAGCCAG 20 0.006941376 52.494286 12 GTTGTTC 20 0.006941376 52.494286 8 CCTGTAC 30 5.86342E-4 46.661587 40 TTAACGG 30 5.86342E-4 46.661587 35 TGGTACA 30 5.86342E-4 46.661587 46 CTGCTAC 40 4.5754172E-5 43.745235 67 GTTCTTA 35 0.0012061014 40.30283 1 GCCACTA 35 0.0012523785 39.995644 40 TATGTCA 35 0.0012523785 39.995644 52 GTCGCCC 40 0.0024129453 34.99619 37 AAGAGCG 40 0.0024129453 34.99619 27 CGGGGCG 40 0.0024129453 34.99619 51 CGCCATT 40 0.0024129453 34.99619 33 AGAAGTC 65 2.091491E-5 32.304173 5 GGAAGAG 55 2.981029E-4 31.814718 6 CGATGGT 55 2.981029E-4 31.814718 32 ATGTAGA 45 0.0042969864 31.107723 61 TAGCAAC 45 0.0042969864 31.107723 66 >>END_MODULE