FastQCFastQC Report
Wed 25 May 2016
SRR1780694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131241
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT12290.9364451657637477No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5630.42898179684702187TruSeq Adapter, Index 12 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2440.18591751053405567No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1490.11353159454743564No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG207.902508E-570.05
CTATGGA150.002220502470.04
CCTATGG150.002220502470.03
GCGCCCG200.006938386752.555
AATATCG200.006938386752.536
ATATCGT200.006938386752.537
CGAGCCT200.006938386752.58
TCCCGAA200.006938386752.532
CCGAATC200.006938386752.534
GCCCGCT200.006938386752.557
CCCGCTC200.006938386752.558
GTAGCCA352.0772826E-550.025
ACGGCTC352.0772826E-550.08
GTGTCCA352.0772826E-550.048
AGTACGG305.860276E-446.6666645
TCTGCAA404.5724766E-543.7529
CAAGGTT404.5724766E-543.7533
CTGCAAG404.5724766E-543.7530
TGCCTGA404.5724766E-543.7513
TAGTGTC404.5724766E-543.7546
Produced by FastQC (version 0.11.5)