FastQCFastQC Report
Wed 25 May 2016
SRR1780693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894103
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCT250.002336219735.24975214
GTCTCAC250.002347166635.2161811
CTCGGTT300.005731331729.34681734
AATCCAG901.6370905E-1129.29435
CCGTGCG1200.027.466489
CGGTCGT655.9045306E-727.08936910
TCTCGGG655.9800914E-727.04996943
TTCGGTA1650.026.65055526
GGTCGGT1006.002665E-1126.41213411
CGTGCGA1200.025.67846510
TGACCGT609.812102E-625.6454241
GTAATCC1200.025.6325133
CGGTAAG1550.025.53295128
AATTCAG959.440555E-1025.51397918
CCCGTGC1300.025.3536728
CATCGTT801.3181307E-724.73227932
TGACGCG450.001389645524.46938912
GCCTGGA450.001403773424.42694331
ATCCAGG1102.0372681E-1023.9680616
CCGGTCG752.085264E-623.438069
Produced by FastQC (version 0.11.5)