FastQCFastQC Report
Wed 25 May 2016
SRR1780693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894103
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT128551.4377538158355356No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT50240.5619039417158873TruSeq Adapter, Index 7 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC21660.24225396850251035No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC16190.18107533472094378No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG12200.13644960368100767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCA207.8515103E-444.00458510
TGCGGAA207.855837E-443.99966416
GCGATTC207.855837E-443.99966427
CCGTCTT6500.037.22840544
CCGCTCG250.002353199835.1977638
TAGTGCC250.002353199835.1977635
GCCGTCT7100.034.0823443
CGTTGAG608.00901E-932.99790630
GCCGTTG608.00901E-932.99790628
TACATGT353.2183965E-431.4265737
TATAAGC353.2183965E-431.42657344
TGCCGTC7850.030.82606542
ATGATTC2900.030.34289740
ATTGGCG3500.029.54263121
GTGTACA300.00572092429.3577371
CTCGGAA300.005741297729.33639114
AGGTCCA300.00574443729.333115
CGGAAAC300.005746007429.3314718
ATAGAAC300.005746007429.3314738
TGAGTAC300.005746007429.3314743
Produced by FastQC (version 0.11.5)