FastQCFastQC Report
Wed 25 May 2016
SRR1780692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147561
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC200.006943282752.493924
GTCAGTG200.006943282752.493917
GGGAACC200.006943282752.493970
CGGTATG200.006943282752.493943
TCTGGTC200.006943282752.493910
TTTAGCG305.8660243E-446.66124726
GGCTCGT404.362771E-544.1036071
TCCGAAG350.00125293139.99535467
TTCCGAA350.00125293139.99535466
CCCGTGC350.00125293139.9953548
CTGTTGG350.00125293139.99535442
CCGAAGC459.1692345E-538.88437368
CTACTGG501.7044452E-434.9959379
GTAACCA501.7044452E-434.99593733
CGTGCGA400.002414003534.99593410
CTCCAAT400.002414003534.99593412
CGCGAGC400.002414003534.99593414
CACTCAG400.002414003534.99593427
TCGCTGA400.002414003534.99593416
TCTCGCG400.002414003534.99593411
Produced by FastQC (version 0.11.5)