##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780692_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147561 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.10292014827766 34.0 31.0 34.0 12.0 34.0 2 31.677638400390347 34.0 33.0 34.0 23.0 34.0 3 31.751153760139875 34.0 33.0 34.0 23.0 34.0 4 32.2088559985362 34.0 34.0 34.0 27.0 34.0 5 32.17596112794031 34.0 34.0 34.0 27.0 34.0 6 34.80124829731433 38.0 36.0 38.0 27.0 38.0 7 34.35160374353657 38.0 35.0 38.0 24.0 38.0 8 32.95283984250581 37.0 33.0 38.0 21.0 38.0 9 33.88390563902386 37.0 34.0 38.0 23.0 38.0 10-11 34.35674737905002 37.0 34.5 38.0 25.0 38.0 12-13 34.77973854880355 38.0 35.5 38.0 26.0 38.0 14-15 34.37604787172762 38.0 34.5 38.0 25.0 38.0 16-17 34.254077974532564 38.0 34.0 38.0 24.0 38.0 18-19 34.21659517081071 37.5 34.5 38.0 24.5 38.0 20-21 34.28590887836217 38.0 34.0 38.0 24.0 38.0 22-23 34.324699615752124 37.5 34.5 38.0 25.0 38.0 24-25 34.757767973922654 38.0 35.5 38.0 26.5 38.0 26-27 34.84032366275642 38.0 36.0 38.0 26.5 38.0 28-29 34.98626669648485 38.0 36.0 38.0 27.0 38.0 30-31 34.81956614552625 38.0 36.0 38.0 27.0 38.0 32-33 34.53730321697468 38.0 35.0 38.0 25.0 38.0 34-35 34.37911778857557 37.5 34.5 38.0 25.0 38.0 36-37 34.37636299564248 37.5 34.5 38.0 25.0 38.0 38-39 34.965773476731655 38.0 36.0 38.0 27.0 38.0 40-41 34.424089020811735 38.0 34.5 38.0 24.5 38.0 42-43 34.78283896151422 38.0 36.0 38.0 26.5 38.0 44-45 33.7849228454673 37.0 34.0 38.0 23.0 38.0 46-47 34.53584619242211 37.0 35.0 38.0 26.0 38.0 48-49 34.23744078719987 37.0 34.0 38.0 24.0 38.0 50-51 34.20998095702794 37.0 34.0 38.0 25.0 38.0 52-53 34.24798557884536 37.0 34.0 38.0 24.0 38.0 54-55 33.980679176747245 37.0 34.0 38.0 23.5 38.0 56-57 34.08081742465828 37.0 34.0 38.0 24.0 38.0 58-59 33.94278298466397 37.0 34.0 38.0 23.5 38.0 60-61 33.929984887605805 37.0 34.0 38.0 23.5 38.0 62-63 34.0858865147295 37.0 34.0 38.0 24.0 38.0 64-65 34.08729610127337 37.0 34.0 38.0 24.0 38.0 66-67 33.96494670000881 37.0 34.0 38.0 23.5 38.0 68-69 33.538445117612376 37.0 33.5 38.0 22.5 38.0 70-71 33.34801539702225 37.0 33.0 38.0 21.5 38.0 72-73 33.44137678654929 37.0 33.0 38.0 22.5 38.0 74-75 33.00699371785228 37.0 32.0 38.0 21.0 38.0 76 32.22226062441973 36.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.468278025096865 1101 2 -1.114118699692245 1101 3 -0.4811988413198023 1101 4 -2.681769177233381 1101 5 -1.417995576696626 1101 6 -3.5746751307570577 1101 7 -2.1623228824759195 1101 8 -1.338174612585508 1101 9 -1.707258140065619 1101 10-11 -2.0408290394204656 1101 12-13 -1.5742083528711603 1101 14-15 -0.8263616833523244 1101 16-17 -2.2186582136949227 1101 18-19 -0.7116659102862286 1101 20-21 -0.8349794977482858 1101 22-23 -0.911199582840645 1101 24-25 -1.6544919203023483 1101 26-27 -1.550594302288637 1101 28-29 -0.6420390837512642 1101 30-31 -1.499151959885829 1101 32-33 0.12783232730050997 1101 34-35 -0.9833116692759134 1101 36-37 -0.5152029660174549 1101 38-39 -1.1146350990449676 1101 40-41 -0.5510660267703855 1101 42-43 -1.5040603979862226 1101 44-45 -0.3183383774822133 1101 46-47 0.2470148468404787 1101 48-49 -0.971243576625838 1101 50-51 -1.2413920353681718 1101 52-53 -0.5939840225519362 1101 54-55 -1.6695736371150787 1101 56-57 -0.9561677390155978 1101 58-59 -0.5115826373299655 1101 60-61 -0.16349628564598362 1101 62-63 -1.7977081881901213 1101 64-65 -0.3810131304476627 1101 66-67 -1.2540943795238348 1101 68-69 -0.0013826095108910863 1101 70-71 -0.07272786577133417 1101 72-73 -0.5329470253752149 1101 74-75 -1.6830229532465069 1101 76 -2.025697298443589 1102 1 0.5540375880784971 1102 2 -0.6546558872116286 1102 3 0.2381501444751244 1102 4 -0.23176607568881025 1102 5 -0.0996530421144044 1102 6 0.18676956870326933 1102 7 1.0901738609022829 1102 8 1.4794080435772585 1102 9 1.1475818202346062 1102 10-11 -0.392137115842516 1102 12-13 -4.779926050284473 1102 14-15 -1.6235167915962307 1102 16-17 -3.0048958850552587 1102 18-19 -1.0323501110181965 1102 20-21 -0.31623453775821275 1102 22-23 -1.065295947830407 1102 24-25 -0.08427295125576961 1102 26-27 -5.631544615544641 1102 28-29 -0.6445358271294666 1102 30-31 0.10340448822532977 1102 32-33 -7.800746855132342 1102 34-35 -0.5159360412131448 1102 36-37 0.026128992297785203 1102 38-39 0.19403837034278837 1102 40-41 0.33642156705133175 1102 42-43 0.13383815049091652 1102 44-45 -6.044886823608387 1102 46-47 -0.537392913224032 1102 48-49 -0.3063344518817246 1102 50-51 -7.07715749656785 1102 52-53 -1.8485665546529262 1102 54-55 0.6394099867295964 1102 56-57 -1.7808451783804031 1102 58-59 0.6438280071709954 1102 60-61 0.22744953037039295 1102 62-63 -4.612189299783701 1102 64-65 -1.046883734008233 1102 66-67 -0.05739442294546393 1102 68-69 -7.724352648590351 1102 70-71 -0.2950427035605543 1102 72-73 -0.09344652912808726 1102 74-75 -7.180731687196012 1102 76 -2.5257593293349707 1103 1 -0.1727328919297264 1103 2 -0.40493854730002354 1103 3 -0.255335825305945 1103 4 0.07838913039608997 1103 5 -0.25963865214626836 1103 6 -1.9310996881576585 1103 7 0.28202297180781954 1103 8 0.895406604580451 1103 9 -0.09133019538878528 1103 10-11 0.30684916229500914 1103 12-13 -1.42604846779912 1103 14-15 -1.0904776817185464 1103 16-17 -0.7895410181624669 1103 18-19 -0.9320571723811355 1103 20-21 -0.9168551051341041 1103 22-23 -0.8504293119993847 1103 24-25 -0.024271923400903006 1103 26-27 -1.041119083629745 1103 28-29 0.1261754484384241 1103 30-31 -0.2949470895318953 1103 32-33 -5.352101053919473 1103 34-35 -1.6352250226089708 1103 36-37 -0.1838167883579871 1103 38-39 -0.05777322386011008 1103 40-41 0.36361475237356444 1103 42-43 0.295847349791984 1103 44-45 -5.102735780335696 1103 46-47 -0.8063118668677802 1103 48-49 -0.20426846359926287 1103 50-51 -0.6264816819928711 1103 52-53 -0.4271589074127178 1103 54-55 -0.19349935647114336 1103 56-57 0.6787986699082182 1103 58-59 0.20173478073456863 1103 60-61 0.7552337322410878 1103 62-63 0.05334672652938366 1103 64-65 0.45052093245286073 1103 66-67 -0.44900712723161007 1103 68-69 -3.2736639858295327 1103 70-71 -0.11422376019535818 1103 72-73 0.5735092217198954 1103 74-75 -2.1148654625069376 1103 76 0.14311722529551218 1104 1 -1.6104116774345982 1104 2 0.02023563874372769 1104 3 0.19577338667283328 1104 4 0.23096067427198363 1104 5 -0.1872164275501298 1104 6 -0.5253559998052779 1104 7 0.7571977513304304 1104 8 1.2664199103763636 1104 9 -0.7117351853589895 1104 10-11 -0.25245717962003056 1104 12-13 -0.7997977111135839 1104 14-15 0.22890296374905716 1104 16-17 -0.6110730244770792 1104 18-19 0.2978684053705365 1104 20-21 1.1836772453982505 1104 22-23 0.26915370975754627 1104 24-25 0.6083142200548082 1104 26-27 0.9422961497854843 1104 28-29 0.02320686720897669 1104 30-31 -0.6631443381639244 1104 32-33 -1.0051681526387455 1104 34-35 -0.10441753527974384 1104 36-37 0.3210052273336004 1104 38-39 -0.10663730533288884 1104 40-41 0.8704961396897346 1104 42-43 0.622020360747598 1104 44-45 -3.755473498290808 1104 46-47 0.15268171169101663 1104 48-49 0.9155015039967935 1104 50-51 1.0307872470151906 1104 52-53 -0.3427573121415577 1104 54-55 -0.24854155281769152 1104 56-57 0.5463608961660924 1104 58-59 1.0478546159848392 1104 60-61 0.12506898251112375 1104 62-63 0.6064185730794946 1104 64-65 0.06117209745185903 1104 66-67 0.1922527096803961 1104 68-69 -3.9771886567455077 1104 70-71 -1.0427065908521058 1104 72-73 0.4886180063938994 1104 74-75 -1.228169477984867 1104 76 -0.11321816147465569 1105 1 -0.5209509865419264 1105 2 0.5486143103204917 1105 3 -0.3020481493348619 1105 4 0.6571538450952232 1105 5 0.47633842693551287 1105 6 1.0584371663267973 1105 7 1.3676074667520766 1105 8 0.9639330016842038 1105 9 0.9923807335540644 1105 10-11 1.3137323555432587 1105 12-13 -0.9539790132662418 1105 14-15 0.3234535785860686 1105 16-17 1.577427148509969 1105 18-19 1.0608175119950545 1105 20-21 0.09607250027560355 1105 22-23 0.7611112780874336 1105 24-25 1.0531870599157145 1105 26-27 0.815086166966914 1105 28-29 -0.31039425398164866 1105 30-31 0.4100585023420251 1105 32-33 0.9366788799004127 1105 34-35 0.8227725100487291 1105 36-37 1.3172698799228328 1105 38-39 0.7551958882425467 1105 40-41 0.8605449865404893 1105 42-43 1.0081684962990778 1105 44-45 1.9630062094490413 1105 46-47 1.1544614258812302 1105 48-49 0.4477152104348363 1105 50-51 0.4510695384191976 1105 52-53 -0.6045394891981957 1105 54-55 -0.3847673859408971 1105 56-57 0.15220844473441275 1105 58-59 0.5665987886669654 1105 60-61 0.4909600417196103 1105 62-63 0.7408676239963299 1105 64-65 0.6756572704095802 1105 66-67 0.5793038962542596 1105 68-69 1.3690815666130263 1105 70-71 1.9065612927833229 1105 72-73 1.1475813741878156 1105 74-75 -0.1335924752933053 1105 76 -0.2958045230586279 1106 1 -0.3039468548407456 1106 2 0.3027909212990103 1106 3 -0.9364952455958004 1106 4 -0.72112777781647 1106 5 0.10825132759504896 1106 6 -0.8778037554788369 1106 7 0.6002402072761086 1106 8 0.27447782166007784 1106 9 0.7177316715272184 1106 10-11 0.1742521543473714 1106 12-13 0.25353311237852694 1106 14-15 0.15260709309834652 1106 16-17 0.24760640275073342 1106 18-19 -0.061147868968319585 1106 20-21 -0.3738443524459285 1106 22-23 -0.2025214111111211 1106 24-25 0.03560808283253891 1106 26-27 0.1806551099186393 1106 28-29 -1.2419677649011405 1106 30-31 -0.8275752646916246 1106 32-33 0.786904712014767 1106 34-35 0.5240004721705205 1106 36-37 0.5064395619614857 1106 38-39 0.5354362198051561 1106 40-41 0.19238988093831466 1106 42-43 0.4370153410651625 1106 44-45 1.2198838238407106 1106 46-47 0.8599846891552829 1106 48-49 0.30373761400709043 1106 50-51 0.5013428466030447 1106 52-53 0.46560640348022275 1106 54-55 0.26103547288836637 1106 56-57 0.9330646820634314 1106 58-59 -0.47776888141284957 1106 60-61 1.0096361009492085 1106 62-63 0.4904473966244467 1106 64-65 0.8449030424731205 1106 66-67 1.0508759957230325 1106 68-69 1.5905638464908733 1106 70-71 0.41976798049343245 1106 72-73 0.7218846307895532 1106 74-75 -1.3613396716389303 1106 76 -0.4186328136634998 1107 1 -0.7719911275226075 1107 2 0.26856109244815585 1107 3 0.5382398807285718 1107 4 0.24097491167007945 1107 5 -0.12118974294153873 1107 6 0.6566363949010565 1107 7 0.3899449684297238 1107 8 0.8105348187944514 1107 9 -0.6367455480221693 1107 10-11 0.2741253041751648 1107 12-13 0.5438852694887188 1107 14-15 -0.31281801929221587 1107 16-17 1.1328069969090535 1107 18-19 1.355193456583116 1107 20-21 0.544228839446177 1107 22-23 -0.4610295742154662 1107 24-25 0.08341679540188096 1107 26-27 0.6037464420426275 1107 28-29 -0.6295147586177876 1107 30-31 0.15273666842613665 1107 32-33 1.1173395517760554 1107 34-35 0.8931344732747064 1107 36-37 0.02533892310983532 1107 38-39 0.8184271233990259 1107 40-41 0.9401092068578123 1107 42-43 0.6700220187737074 1107 44-45 0.7614551885645326 1107 46-47 0.49915510217418557 1107 48-49 1.0715722224147015 1107 50-51 1.3160758697121295 1107 52-53 1.473385013780721 1107 54-55 0.9902202140614378 1107 56-57 1.5740096829835863 1107 58-59 1.2142579608722883 1107 60-61 0.06142120492691561 1107 62-63 -0.22555609997097292 1107 64-65 0.8482418967465861 1107 66-67 1.5953392814711265 1107 68-69 1.7744768785386142 1107 70-71 0.8473680803961656 1107 72-73 1.3179194127124205 1107 74-75 0.6060309148952356 1107 76 0.05731823400061842 1108 1 -1.397961278543523 1108 2 0.11877155083896085 1108 3 -0.3291506128274371 1108 4 0.16386011564600267 1108 5 0.11790401316353183 1108 6 0.8022582091648687 1108 7 1.063204616659327 1108 8 1.2994198925288885 1108 9 -0.1959669407771898 1108 10-11 0.16792225770856106 1108 12-13 0.4428149213295569 1108 14-15 0.7066471618322439 1108 16-17 -1.0814343168722758 1108 18-19 0.14009077301662387 1108 20-21 0.6386331918796131 1108 22-23 -0.13867895231240368 1108 24-25 -0.6884149326065483 1108 26-27 -0.8141215049372548 1108 28-29 0.3406658626340757 1108 30-31 0.4666038339571301 1108 32-33 0.6432990466759136 1108 34-35 0.6437575603529879 1108 36-37 0.604824706068328 1108 38-39 0.9358664575098601 1108 40-41 0.32549670485978766 1108 42-43 0.6132988943990156 1108 44-45 0.3347722614464814 1108 46-47 0.8951099202209178 1108 48-49 0.719372621427226 1108 50-51 0.5890176103302949 1108 52-53 0.11343308472680746 1108 54-55 0.6871986117360365 1108 56-57 0.9856467591807672 1108 58-59 -0.6708453739015852 1108 60-61 1.0400912026180933 1108 62-63 0.46517662675280036 1108 64-65 0.6065344309497789 1108 66-67 0.4333840016591495 1108 68-69 1.4460404275005274 1108 70-71 2.37768474479347 1108 72-73 0.6533948237130858 1108 74-75 1.4596365318550397 1108 76 1.2990049795620493 1109 1 -0.2227872108627622 1109 2 -0.47711338492108624 1109 3 0.19189390910914383 1109 4 -0.2737593567638399 1109 5 0.17887693854324027 1109 6 0.3858686219456615 1109 7 -0.7335561604761125 1109 8 0.6958134187172362 1109 9 1.3796265112658617 1109 10-11 0.9145220005170458 1109 12-13 0.0994005301899179 1109 14-15 -0.14035864235464857 1109 16-17 -0.30459429694572293 1109 18-19 -0.07365622931199312 1109 20-21 0.6090619602566463 1109 22-23 0.7086338597640136 1109 24-25 0.7153554718329076 1109 26-27 0.3918952704372387 1109 28-29 0.010533907167591394 1109 30-31 0.3077272511287177 1109 32-33 1.016556921717907 1109 34-35 0.44042275956055477 1109 36-37 0.37040390831122494 1109 38-39 0.5092297579025953 1109 40-41 0.5130669401552694 1109 42-43 0.6034311974215996 1109 44-45 0.16457693491751968 1109 46-47 -0.17413194407000532 1109 48-49 -0.16085954604847075 1109 50-51 0.9392962344715485 1109 52-53 1.1109799179114646 1109 54-55 0.2540770945377986 1109 56-57 0.47087272857266527 1109 58-59 0.3452812292009284 1109 60-61 1.068428610081277 1109 62-63 -0.3374259891861229 1109 64-65 1.9964174255449052E-4 1109 66-67 0.2813513777264376 1109 68-69 1.1750161796698535 1109 70-71 0.4285206172172096 1109 72-73 -0.5197228703680352 1109 74-75 1.6385381387227511 1109 76 -0.41555877960474774 1110 1 0.6232405257607532 1110 2 0.36370462688276817 1110 3 -0.1403869545636489 1110 4 0.428618415076933 1110 5 0.4624486967342847 1110 6 0.1616318648475641 1110 7 0.7631407039738889 1110 8 2.3221826412659965 1110 9 -1.3611259235822217 1110 10-11 -0.9407047257355927 1110 12-13 0.27778806571077297 1110 14-15 0.043408632315639295 1110 16-17 0.7014776434035355 1110 18-19 0.8495215813891903 1110 20-21 0.16520919266623224 1110 22-23 0.7512406651947572 1110 24-25 0.8124761577192885 1110 26-27 -1.0347739059647694 1110 28-29 0.47691357345374996 1110 30-31 -0.12004701841685517 1110 32-33 1.5603477555167586 1110 34-35 1.0129885186745398 1110 36-37 -0.057728605714956416 1110 38-39 0.6096475073986269 1110 40-41 0.49596228419140687 1110 42-43 0.5309990209952957 1110 44-45 1.6861383950977356 1110 46-47 0.6993834665145968 1110 48-49 1.0680040295957625 1110 50-51 0.4954070352390403 1110 52-53 0.5799219430250062 1110 54-55 -0.11084652045260412 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48-49 0.9131489874690146 2116 50-51 0.3065889390539951 2116 52-53 1.3199462771084285 2116 54-55 0.6104562688089956 2116 56-57 0.03687800652856765 2116 58-59 -0.8066819730878336 2116 60-61 -2.1151283375831014 2116 62-63 0.6776494806506079 2116 64-65 0.9782862594113766 2116 66-67 -0.10721346220845618 2116 68-69 0.5206298037018158 2116 70-71 -2.9219208566643076 2116 72-73 0.16451848512942746 2116 74-75 -2.0027458350354514 2116 76 -1.0684221939982734 2117 1 -0.22446185201108904 2117 2 0.005422500246378803 2117 3 0.6880661579129743 2117 4 0.18413538007826702 2117 5 -0.9689699514089156 2117 6 -1.15264812446582 2117 7 -2.7433647729163084 2117 8 -2.9462918444406547 2117 9 -0.06845961313604931 2117 10-11 -0.6347602961336705 2117 12-13 0.4320560325822669 2117 14-15 -0.546459120823549 2117 16-17 0.05975977003992483 2117 18-19 0.3116308520277187 2117 20-21 -0.4966796665376094 2117 22-23 0.14140897938123942 2117 24-25 0.22468561537357346 2117 26-27 1.2549335479492 2117 28-29 0.5482133330049166 2117 30-31 -0.05232059576123049 2117 32-33 1.0068679265025935 2117 34-35 -0.41161073326241393 2117 36-37 -1.3309118811624572 2117 38-39 0.3598278440270022 2117 40-41 -1.6067895191748676 2117 42-43 -1.0050616255449114 2117 44-45 0.7406534899414439 2117 46-47 -0.24087835555325654 2117 48-49 -0.4719839479623431 2117 50-51 0.7578369043280091 2117 52-53 1.0307167600167233 2117 54-55 -0.9796549628784703 2117 56-57 -0.6560411470254195 2117 58-59 -1.00424030189955 2117 60-61 0.8564668875932355 2117 62-63 -0.47187574995167125 2117 64-65 -0.2962118415110311 2117 66-67 -2.1318065170104106 2117 68-69 -0.21684984903827598 2117 70-71 0.838089995207639 2117 72-73 0.9991766511630686 2117 74-75 -0.005241765583470226 2117 76 -0.8543147604659893 2118 1 0.11909686229292049 2118 2 0.030284838573948036 2118 3 0.045256579221717885 2118 4 0.017315044567958182 2118 5 -0.17259397497146978 2118 6 0.6762736324812906 2118 7 0.3766313853626002 2118 8 -4.336777181871824 2118 9 -1.3994687052093013 2118 10-11 -1.0328842556675468 2118 12-13 0.3708414084306426 2118 14-15 -1.2495100876566951 2118 16-17 -0.5259298188959178 2118 18-19 -0.8556793029217005 2118 20-21 -3.3846166623117178 2118 22-23 -0.8982183736729255 2118 24-25 -0.49598284409626814 2118 26-27 0.3536497728179455 2118 28-29 -1.049606490275913 2118 30-31 -1.0064760574080438 2118 32-33 -0.26986084798837595 2118 34-35 0.23377599999408716 2118 36-37 -0.7820483903185647 2118 38-39 -0.6706209970538097 2118 40-41 -1.9143128896448403 2118 42-43 -0.8384045631809727 2118 44-45 -0.4383258928220357 2118 46-47 -0.8426583529921103 2118 48-49 -1.4149952170108833 2118 50-51 -1.0182003603665777 2118 52-53 -1.8206054323254435 2118 54-55 0.012546343306702568 2118 56-57 0.4651363404889821 2118 58-59 0.29023402259782927 2118 60-61 -1.9067166186175406 2118 62-63 -0.24651398788225976 2118 64-65 -0.5292826572364788 2118 66-67 -2.7159594175098327 2118 68-69 0.8691918976642441 2118 70-71 -1.322314666080615 2118 72-73 -1.8280035255561025 2118 74-75 -0.0873521510361499 2118 76 -0.9270897636674249 2119 1 -0.12106420695505449 2119 2 0.42408472183809565 2119 3 0.11157303832154142 2119 4 -0.09713855948763239 2119 5 0.4944858579257385 2119 6 0.7796897958065614 2119 7 -1.5319801793569354 2119 8 0.18616666031179108 2119 9 -0.5717721397355717 2119 10-11 -0.17348210064495362 2119 12-13 0.14172761720419658 2119 14-15 -0.8084289037714925 2119 16-17 -1.0421908612552926 2119 18-19 -0.9542260071012407 2119 20-21 -1.7742225804261054 2119 22-23 0.40017618040266 2119 24-25 -0.49466464707499824 2119 26-27 -0.32885493268634747 2119 28-29 0.33004408914053585 2119 30-31 -0.09849735842259832 2119 32-33 -0.46716739765685844 2119 34-35 0.02906524914643427 2119 36-37 -0.8725383530024402 2119 38-39 -0.5374306323526312 2119 40-41 -0.2413405193167364 2119 42-43 -0.8210745351774875 2119 44-45 -1.73156242720993 2119 46-47 -0.7414883711583116 2119 48-49 -0.9025379272501937 2119 50-51 0.8157068149536286 2119 52-53 -1.2415168901464142 2119 54-55 -1.9271510616158771 2119 56-57 0.5408342962998773 2119 58-59 0.9573925538246613 2119 60-61 0.2465647738182426 2119 62-63 -0.5607039525335935 2119 64-65 0.030423142174598183 2119 66-67 0.44421135786546273 2119 68-69 -0.9388104108200892 2119 70-71 -1.3196061319025851 2119 72-73 -2.300646018719185 2119 74-75 -0.5674775437080228 2119 76 0.4326337428678144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 13.0 13 107.0 14 289.0 15 520.0 16 722.0 17 790.0 18 819.0 19 865.0 20 864.0 21 856.0 22 863.0 23 1086.0 24 1214.0 25 1502.0 26 1865.0 27 2281.0 28 2792.0 29 3461.0 30 4274.0 31 5473.0 32 6784.0 33 9229.0 34 12815.0 35 18885.0 36 31340.0 37 37847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.93325775631876 21.7163266839219 11.098211419869015 30.25220413989032 2 18.337501101239486 20.484409837287632 35.117680145837994 26.06040891563489 3 17.426691334431187 23.756277065078173 28.301515983220497 30.515515617270143 4 13.081369738616571 17.243038472225045 38.68298534165531 30.992606447503068 5 12.850278867722501 36.47847330934326 36.483217110212045 14.188030712722197 6 28.683730796077555 37.51465495625538 18.108443287860613 15.693170959806451 7 28.875515888344484 29.38174720962856 22.126442623728494 19.616294278298465 8 23.981268763426648 33.75756466817113 20.2262115328576 22.034955035544623 9 23.30561598254281 16.133666754765827 22.419880591755273 38.14083667093609 10-11 24.549169496005042 25.455235461944554 27.574359078618333 22.421235963432075 12-13 23.86775638549481 23.887409274808384 26.63644187827407 25.608392461422735 14-15 21.613773286979622 25.394921422327037 23.90435142076836 29.08695386992498 16-17 21.885525308177638 27.91218546906025 26.702177404598775 23.500111818163337 18-19 21.232575002880164 27.293797141521132 27.34055746437067 24.133070391228035 20-21 21.070608087502794 27.929127615020228 26.690995588265192 24.309268709211786 22-23 22.327037631894605 27.16097071719492 27.312772344996304 23.199219305914166 24-25 22.055624453615792 28.0199375173657 26.74182202614512 23.182616002873388 26-27 20.46170736170126 28.335400275140447 27.663813609287008 23.53907875387128 28-29 21.643930306788377 27.287020283137142 28.49770603343702 22.571343376637458 30-31 21.35625266838799 27.306334329531516 27.876268119625102 23.461144882455393 32-33 20.61926931912904 28.10024328921599 27.571309492345534 23.709177899309438 34-35 21.646979893061175 27.715656575924534 27.636367332831846 23.000996198182445 36-37 22.21826905483156 27.270078137177165 27.406631833614576 23.105020974376696 38-39 21.315930361003247 27.889821836393086 28.088722629963204 22.705525172640467 40-41 21.86180630383367 26.21966508765866 28.210706080875024 23.70782252763264 42-43 21.063492386199606 26.143086587919573 28.366573823706805 24.426847202174017 44-45 20.501690826166804 25.60161560303874 28.99682165341791 24.899871917376544 46-47 20.61926931912904 25.345789199043107 29.753457891990433 24.281483589837425 48-49 19.43738521696112 25.94994612397585 30.415556956106286 24.197111702956743 50-51 19.461781907143486 26.32707829304491 29.626730640209814 24.58440915960179 52-53 18.838649778735576 26.393152662288816 29.906953734387816 24.8612438245878 54-55 18.116914360840603 26.744871612417914 29.60063973543145 25.537574291310033 56-57 18.39171596831141 26.695061703295586 29.398350512669335 25.514871815723666 58-59 18.61602998082149 26.176970879839523 29.30211912361667 25.90488001572231 60-61 18.851864652584354 25.99128496011819 29.54100338165233 25.615847005645122 62-63 18.910145634686675 26.32199564925692 28.902962164799646 25.864896551256766 64-65 20.04831900027785 25.96824364161262 27.658053279660617 26.32538407844891 66-67 20.10524461070337 25.80119408244726 26.953598850644816 27.139962456204554 68-69 19.877203326082093 25.275987557688005 27.14301204247735 27.70379707375255 70-71 20.922194888893404 25.086235522936278 27.07795420199104 26.913615386179274 72-73 21.78963276204417 25.40034290903423 26.155623775929953 26.654400552991643 74-75 22.245376488367523 25.69513624873781 25.8408387039936 26.218648558901066 76 22.912558196271373 25.189582613292128 25.963499840743825 25.93435934969267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 1.0 14 2.0 15 2.0 16 5.0 17 8.0 18 10.5 19 16.0 20 26.0 21 33.0 22 40.5 23 69.5 24 116.5 25 142.0 26 235.0 27 395.0 28 597.5 29 733.0 30 872.5 31 1170.5 32 1597.0 33 1865.0 34 2255.5 35 3089.0 36 3971.0 37 4410.0 38 4990.5 39 6276.0 40 7853.5 41 9139.5 42 9553.0 43 9714.0 44 10487.0 45 11196.5 46 11294.0 47 11377.5 48 11030.0 49 10074.0 50 9549.0 51 9052.0 52 7707.5 53 6204.5 54 5549.0 55 5056.0 56 3924.5 57 2830.0 58 2374.0 59 1956.5 60 1358.0 61 956.5 62 736.0 63 623.5 64 414.5 65 276.5 66 192.0 67 149.0 68 123.5 69 79.0 70 51.5 71 43.0 72 33.0 73 19.5 74 11.5 75 7.0 76 6.0 77 7.0 78 7.5 79 6.0 80 7.0 81 5.5 82 3.0 83 3.0 84 3.0 85 3.0 86 4.0 87 5.0 88 4.0 89 3.5 90 3.5 91 2.5 92 2.0 93 2.0 94 1.5 95 1.5 96 2.0 97 1.5 98 1.5 99 6.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7671403690677076 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147561.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.0370829690772 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03645392472208 35.564952799181356 2 9.579030528751199 9.202973685458895 3 5.033575983296654 7.253949214223271 4 2.941425427458947 5.651899892247951 5 1.8537328593194518 4.4523959582816595 6 1.1878562157891766 3.423668855591925 7 0.9127588736527285 3.0692391621092296 8 0.7208961119575644 2.770379707375255 9 0.5431409062693979 2.348181430052656 >10 3.164324812369505 25.487086696349305 >50 0.02680435641329496 0.775272599128496 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00677685838399035 0.0 2 0.0 0.0 0.0 0.007454544222389385 0.0 3 0.0 0.0 0.0 0.007454544222389385 0.0 4 0.0 0.0 0.0 0.01084297341438456 0.0 5 0.0 0.0 0.0 0.011520659252783595 0.0 6 0.0 0.0 0.0 0.012876030929581665 0.0 7 0.0 0.0 0.0 0.012876030929581665 0.0 8 0.0 0.0 0.0 0.012876030929581665 0.0 9 0.0 0.0 0.0 0.02033057515197105 0.0 10 0.0 0.0 0.0 0.031851234404754646 0.0 11 0.0 0.0 0.0 0.037272721111946926 0.0 12 0.0 0.0 0.0 0.038628092788744996 0.0 13 0.0 0.0 0.0 0.039983464465543066 0.0 14 0.0 0.0 0.0 0.041338836142341136 0.0 15 0.0 0.0 0.0 0.04608263701113438 0.0 16 0.0 0.0 0.0 0.04743800868793245 0.0 17 0.0 0.0 0.0 0.050826437879927626 0.0 18 0.0 0.0 0.0 0.053537181233523766 0.0 19 0.0 0.0 0.0 0.058958667940716046 0.0 20 0.0 0.0 0.0 0.058958667940716046 0.0 21 0.0 0.0 0.0 0.06099172545591315 0.0 22 0.0 0.0 0.0 0.06438015464790832 0.0 23 0.0 0.0 0.0 0.07115701303189867 0.0 24 0.0 0.0 0.0 0.07522312806229288 0.0 25 0.0 0.0 0.0 0.07928924309268709 0.0 26 0.0 0.0 0.0 0.08674378731507648 0.0 27 0.0 0.0 0.0 0.11859502171983112 0.0 28 0.0 0.0 0.0 0.16332228705416743 0.0 29 0.0 0.0 0.0 0.19110740642852786 0.0 30 0.0 0.0 0.0 0.23109087089407093 0.0 31 0.0 0.0 0.0 0.2744627645516092 0.0 32 0.0 0.0 0.0 0.3185123440475464 0.0 33 0.0 0.0 0.0 0.3584958085130895 0.0 34 0.0 0.0 0.0 0.41406604726181034 0.0 35 0.0 0.0 0.0 0.5313056973048434 0.0 36 0.0 0.0 0.0 0.7020825285814002 0.0 37 0.0 0.0 0.0 1.0659998238016821 0.0 38 0.0 0.0 0.0 1.566809658378569 0.0 39 0.0 0.0 0.0 2.269569872798368 0.0 40 0.0 0.0 0.0 3.0943135381299935 0.0 41 0.0 0.0 0.0 4.120329897466132 0.0 42 0.0 0.0 0.0 5.411999105454694 0.0 43 0.0 0.0 0.0 6.6711393932001 0.0 44 0.0 0.0 0.0 7.837436721084839 0.0 45 0.0 0.0 0.0 8.866163823774574 0.0 46 0.0 0.0 0.0 9.877271094665934 0.0 47 0.0 0.0 0.0 10.83551887016217 0.0 48 0.0 0.0 0.0 11.771403012991238 0.0 49 0.0 0.0 0.0 12.651039231233185 0.0 50 0.0 0.0 0.0 13.551006024627103 0.0 51 0.0 0.0 0.0 14.553981065457675 0.0 52 0.0 0.0 0.0 15.63217923435054 0.0 53 0.0 0.0 0.0 17.026179003937354 0.0 54 0.0 0.0 0.0 18.232459796287635 0.0 55 0.0 0.0 0.0 19.175120797500696 0.0 56 0.0 0.0 0.0 20.043914042328257 0.0 57 0.0 0.0 0.0 20.833418044063134 0.0 58 0.0 0.0 0.0 21.58158320965567 0.0 59 0.0 0.0 0.0 22.244359959609923 0.0 60 0.0 0.0 0.0 22.959996204959307 0.0 61 0.0 0.0 0.0 23.70138451216785 0.0 62 0.0 0.0 0.0 24.484789341357132 0.0 63 0.0 0.0 0.0 25.384078448912653 0.0 64 0.0 0.0 0.0 26.224408888527456 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGATC 20 0.0069432827 52.4939 24 GTCAGTG 20 0.0069432827 52.4939 17 GGGAACC 20 0.0069432827 52.4939 70 CGGTATG 20 0.0069432827 52.4939 43 TCTGGTC 20 0.0069432827 52.4939 10 TTTAGCG 30 5.8660243E-4 46.661247 26 GGCTCGT 40 4.362771E-5 44.103607 1 TCCGAAG 35 0.001252931 39.995354 67 TTCCGAA 35 0.001252931 39.995354 66 CCCGTGC 35 0.001252931 39.995354 8 CTGTTGG 35 0.001252931 39.995354 42 CCGAAGC 45 9.1692345E-5 38.884373 68 CTACTGG 50 1.7044452E-4 34.995937 9 GTAACCA 50 1.7044452E-4 34.995937 33 CGTGCGA 40 0.0024140035 34.995934 10 CTCCAAT 40 0.0024140035 34.995934 12 CGCGAGC 40 0.0024140035 34.995934 14 CACTCAG 40 0.0024140035 34.995934 27 TCGCTGA 40 0.0024140035 34.995934 16 TCTCGCG 40 0.0024140035 34.995934 11 >>END_MODULE