FastQCFastQC Report
Wed 25 May 2016
SRR1780692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147561
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT9110.6173717987815208TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT4110.2785288795820034TruSeq Adapter, Index 5 (96% over 26bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1650.11181816333584078No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGC150.002221052870.036
CAGAAGA200.00694009352.50000423
CGTTAAA200.00694009352.5000041
ATTCCCG200.00694009352.50000431
ACGGCTC200.00694009352.5000045
GGACGTC200.00694009352.50000437
TCCCGGA200.00694009352.50000433
AGCCTTA200.00694009352.5000046
CTGGGTG200.00694009352.50000416
CCCGGAC200.00694009352.50000434
CGGACGT200.00694009352.50000436
ATTGGCG451.6757458E-646.66666821
GAGGCGC451.6757458E-646.66666813
CCGGGAA305.862667E-446.66666447
CGTGCGA305.862667E-446.66666410
CCGTGCG305.862667E-446.6666649
CGAGAAT305.862667E-446.66666414
CATGTAC404.575265E-543.7500043
GCTCGGA404.575265E-543.75000411
CCCGTGC350.001252215640.08
Produced by FastQC (version 0.11.5)