FastQCFastQC Report
Wed 25 May 2016
SRR1780691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653413
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC300.005727355429.35244638
ACTAGTG300.00578526629.2920825
GTCAAGC300.00578697629.2903083
CATCGTT2600.026.20894232
TGTTCGT1900.025.48719410
CCCGCAA450.001384546924.48634315
TTAACGC450.001393133724.46037135
TCGTTGT2950.023.88140334
CGTGCGA2550.023.3064710
CGCAAAG1850.022.61685612
CCACGAC3500.022.5974319
CGTTACA2150.022.54405814
CCAACGT2100.022.00366422
GCGAACT2300.021.9830228
TCGTTCA2600.021.97770141
TTGTTCG2200.021.9697279
CGAGAAT2850.021.64517214
CGTTCAT2650.021.56302842
AAAGCGA2450.021.5376525
CAATCGA1551.8189894E-1221.30419538
Produced by FastQC (version 0.11.5)