FastQCFastQC Report
Wed 25 May 2016
SRR1780691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653413
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT160860.9728966688903499No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT66250.4006863378962183RNA PCR Primer, Index 19 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC30200.18265248912401197No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC20640.12483269455362937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT8950.038.59446344
TTACGGC301.3008894E-436.66883542
CTCGATA301.301121E-436.667732
TACACGG250.002354105735.1967618
GCGAGTT250.002354105735.1967631
GCCGTCT9900.034.89095343
CGAGCAC353.217255E-431.4304315
CGTGCGA2150.029.67617210
CGAGAAT2150.029.67617214
CGCCCAA300.00574395629.33506644
AGTTCCG300.005744805629.3341832
CGTCGAG300.00574650429.33240721
TTACCCG300.00574735329.33151837
TTGTACG1300.028.768329
TGCCGTC12400.027.85648342
ACCCGTG407.037022E-427.4974677
TTGCGAT905.14774E-1026.88885116
GTTGTAC1400.026.7118248
CCGTGCG2450.026.0399939
AGTCCGG609.751333E-625.66818412
Produced by FastQC (version 0.11.5)