FastQCFastQC Report
Wed 25 May 2016
SRR1780690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61345
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAAGG150.00221627769.9885822
GTGTTCA200.00692530752.49143615
TTCAGAT200.00692530752.49143618
CTGAGTT200.00692530752.49143666
GGGACTG200.00692530752.49143635
TAACACG200.00692530752.4914364
CAAGGCC200.00692530752.49143624
GTGTCAA200.00692530752.49143654
GCCACTT200.00692530752.49143633
ATGTTAA200.00692530752.49143625
GTAACAC200.00692530752.4914363
ACAGTTG400.002403594334.9942915
GGACTGT400.002403594334.9942936
TACATAC400.002403594334.9942945
GAATAGT450.00404611231.465351
AGTAGCC450.004280438631.10603749
TTTGGGG500.00716377927.99543251
AGTATAG700.001214508724.9959225
ATATAGT800.002470503822.1240711
GACGAAC3104.5474735E-1116.9327270
Produced by FastQC (version 0.11.5)