FastQCFastQC Report
Wed 25 May 2016
SRR1780690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61345
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT11811.9251772760616188No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5190.8460347216562067RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1280.20865596218110682No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC860.14019072459043116No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT800.1304099763631918No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG780.12714972695411197No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG207.868989E-570.0000123
CGATTAG207.868989E-570.0000152
CGTTCGG207.868989E-570.0000124
TTGCGAT207.868989E-570.0000149
GCGATTA207.868989E-570.0000151
TTCGGTA207.868989E-570.0000126
GTAAGCA207.868989E-570.0000130
ATGCCAT207.868989E-570.0000143
TCGGTAA207.868989E-570.0000127
CCATTGC207.868989E-570.0000146
GGGTACA252.809018E-670.062
GGTAAGC252.3731633E-455.99999629
GTTCGGT252.3731633E-455.99999625
TGCGATT252.3731633E-455.99999650
ATTGCGA252.3731633E-455.99999648
CGGTAAG252.3731633E-455.99999628
GGTACAA200.006920839752.50000463
CCAGGGA200.006920839752.5000044
GTTAGTG200.006920839752.50000430
TCAATCC200.006920839752.50000437