##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780690_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61345 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83506398239465 34.0 34.0 34.0 31.0 34.0 2 33.34451055505746 34.0 34.0 34.0 33.0 34.0 3 33.4639497921591 34.0 34.0 34.0 34.0 34.0 4 33.53277365718478 34.0 34.0 34.0 34.0 34.0 5 33.53988100089657 34.0 34.0 34.0 34.0 34.0 6 37.24924606732415 38.0 38.0 38.0 36.0 38.0 7 36.92282989648708 38.0 37.0 38.0 35.0 38.0 8 36.940076615861116 38.0 37.0 38.0 35.0 38.0 9 36.720042383242316 38.0 37.0 38.0 34.0 38.0 10-11 36.4520987855571 38.0 37.0 38.0 34.0 38.0 12-13 36.35679354470617 38.0 37.0 38.0 34.0 38.0 14-15 36.60393675116147 38.0 37.0 38.0 34.0 38.0 16-17 36.73701198141658 38.0 37.0 38.0 34.0 38.0 18-19 36.75759230581139 38.0 37.0 38.0 34.0 38.0 20-21 36.49951911321216 38.0 37.0 38.0 34.0 38.0 22-23 36.557144021517644 38.0 37.0 38.0 34.0 38.0 24-25 35.90063574863477 38.0 36.5 38.0 32.5 38.0 26-27 36.14147852310702 38.0 37.0 38.0 33.5 38.0 28-29 36.02157470046458 38.0 37.0 38.0 33.0 38.0 30-31 35.759801124786044 38.0 36.5 38.0 32.0 38.0 32-33 36.19665824435569 38.0 37.0 38.0 34.0 38.0 34-35 36.22737794441275 38.0 37.0 38.0 33.5 38.0 36-37 36.35226179802755 38.0 37.0 38.0 34.0 38.0 38-39 35.96641128046296 38.0 36.5 38.0 32.5 38.0 40-41 35.5486918249246 38.0 36.0 38.0 31.0 38.0 42-43 35.95988263102127 38.0 36.5 38.0 32.5 38.0 44-45 36.14974325535904 38.0 37.0 38.0 34.0 38.0 46-47 36.41685548944494 38.0 37.0 38.0 34.0 38.0 48-49 36.217947672996985 38.0 37.0 38.0 34.0 38.0 50-51 36.369826391718966 38.0 37.0 38.0 34.0 38.0 52-53 36.28271252750835 38.0 37.0 38.0 34.0 38.0 54-55 36.47377129350396 38.0 37.0 38.0 34.0 38.0 56-57 36.51411687994131 38.0 37.0 38.0 34.0 38.0 58-59 36.56462629391149 38.0 37.0 38.0 34.0 38.0 60-61 36.21766240117369 38.0 37.0 38.0 33.5 38.0 62-63 36.50519194718396 38.0 37.0 38.0 34.0 38.0 64-65 36.50861520906349 38.0 37.0 38.0 34.0 38.0 66-67 35.92580487407287 38.0 37.0 38.0 32.0 38.0 68-69 35.35658162849458 38.0 36.0 38.0 29.5 38.0 70-71 35.26774798272068 38.0 36.0 38.0 29.0 38.0 72-73 34.912682370201324 38.0 36.0 38.0 26.0 38.0 74-75 35.44914825984188 38.0 36.5 38.0 30.5 38.0 76 34.941070991930886 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4281702378540615 1101 2 0.43563012005449053 1101 3 0.27499883706814643 1101 4 0.25583200869493083 1101 5 0.2748863094696006 1101 6 -1.6819744955504703 1101 7 -0.08172083929717644 1101 8 0.16638691013018558 1101 9 -0.17920350018992792 1101 10-11 -0.3590132075470507 1101 12-13 0.5058728616225352 1101 14-15 0.3677088188992599 1101 16-17 -0.06449579091242441 1101 18-19 -0.4455197770936721 1101 20-21 -0.12800123782337636 1101 22-23 0.42740759874963885 1101 24-25 -1.8447794969876057 1101 26-27 -0.27479055332141655 1101 28-29 0.2676912225126955 1101 30-31 0.8658648724510201 1101 32-33 0.0874160878251331 1101 34-35 0.3287040641967991 1101 36-37 -0.17887870616580415 1101 38-39 -0.06241429658030739 1101 40-41 0.804316533227798 1101 42-43 0.567106666808975 1101 44-45 0.20184285085029074 1101 46-47 0.4559666572665151 1101 48-49 -1.9178812743517355 1101 50-51 -1.5176404952208173 1101 52-53 -1.6549790026178997 1101 54-55 -0.5163256077758049 1101 56-57 -0.7898427445543348 1101 58-59 -1.1962841587271242 1101 60-61 -1.8229054356723609 1101 62-63 -0.8965749952891002 1101 64-65 -1.2164803464273604 1101 66-67 -1.2873180048804613 1101 68-69 -1.5924157684608815 1101 70-71 -0.8402668831184528 1101 72-73 -1.482075639642133 1101 74-75 -1.4286764154975558 1101 76 -1.072428730628971 1102 1 -0.5238544100332589 1102 2 0.29531321864604365 1102 3 0.4441009497442039 1102 4 0.2881383467231018 1102 5 0.1681081404555158 1102 6 0.27189874388614754 1102 7 0.05287423112535805 1102 8 0.4435876143555362 1102 9 0.4988774857255649 1102 10-11 0.8555994685092827 1102 12-13 0.48571441091831247 1102 14-15 0.2255433259415156 1102 16-17 0.4548263921861704 1102 18-19 -0.3287504108964825 1102 20-21 0.38942833964140533 1102 22-23 0.38836710579189315 1102 24-25 -3.8156421730439405 1102 26-27 -1.0083292857157815 1102 28-29 -0.08565384790983899 1102 30-31 1.4243155766763707 1102 32-33 0.6131643272617566 1102 34-35 0.8118026557460922 1102 36-37 -2.547804762503837 1102 38-39 -0.13063612756621978 1102 40-41 0.7900119557630134 1102 42-43 1.1115168780765785 1102 44-45 1.0587090480333856 1102 46-47 1.1394173614918728 1102 48-49 -3.1039288095630013 1102 50-51 -0.5064609177560229 1102 52-53 -0.005991326561563426 1102 54-55 0.5157606598298301 1102 56-57 0.7507382413611623 1102 58-59 0.39557323563906266 1102 60-61 -5.93575754834842 1102 62-63 -2.303397178387698 1102 64-65 0.08105486484024027 1102 66-67 1.1698122768096795 1102 68-69 -0.8157872473341214 1102 70-71 -1.0554517422733838 1102 72-73 -5.275165428374525 1102 74-75 -1.9830338098637554 1102 76 -0.4330801390796779 1103 1 0.5495384471095974 1103 2 0.48424179007461277 1103 3 -0.002506193112942867 1103 4 0.38617406100881624 1103 5 0.28257242616979994 1103 6 0.19118445817186114 1103 7 0.5619813739825048 1103 8 0.6805519000698226 1103 9 0.6811989142969992 1103 10-11 0.12738518279499544 1103 12-13 -0.6469641605102581 1103 14-15 -0.0010638169156322874 1103 16-17 -0.11544146495669594 1103 18-19 0.31544601767493674 1103 20-21 0.17835691106998297 1103 22-23 0.6814921057918895 1103 24-25 -3.3583207444725147 1103 26-27 -0.3052935714300702 1103 28-29 -0.30601099076698546 1103 30-31 0.38860129096208595 1103 32-33 0.8505750415474722 1103 34-35 -0.1553402013967684 1103 36-37 -1.342983333932409 1103 38-39 -0.5972432704233626 1103 40-41 -0.26632732995127384 1103 42-43 -0.38750097906628156 1103 44-45 0.7519233337476692 1103 46-47 0.7229887900632903 1103 48-49 -2.7268752381344328 1103 50-51 -0.7169966320417416 1103 52-53 0.34856224486700427 1103 54-55 -0.13540005445589287 1103 56-57 0.35770252707544614 1103 58-59 -0.3032660500752229 1103 60-61 -4.301114691205562 1103 62-63 -1.358486464101972 1103 64-65 -0.058230849445479294 1103 66-67 -0.20536629461888367 1103 68-69 0.454123466951593 1103 70-71 0.3371375434409032 1103 72-73 -5.0709689998031 1103 74-75 -2.5274088098637577 1103 76 -3.677901567651105 1104 1 0.37790457200267014 1104 2 0.20167549409514152 1104 3 0.2668180156124649 1104 4 0.07260690959734717 1104 5 0.02368448776090304 1104 6 0.21606042053285535 1104 7 0.16092063831099068 1104 8 0.47603521914595603 1104 9 0.8794538330309578 1104 10-11 -0.05181071113143787 1104 12-13 0.34398536900214083 1104 14-15 0.5554460205522957 1104 16-17 0.4124798352999406 1104 18-19 0.47250333161847635 1104 20-21 -0.30241297772386133 1104 22-23 0.1899950998038662 1104 24-25 0.7447245934231219 1104 26-27 0.5957500556015773 1104 28-29 1.1313371700542305 1104 30-31 0.8791059958380458 1104 32-33 0.34323543504618215 1104 34-35 0.06419786532693195 1104 36-37 0.9449721836039515 1104 38-39 1.1124402197391703 1104 40-41 1.5191474348049283 1104 42-43 0.8519173271783771 1104 44-45 0.8132749162968622 1104 46-47 0.9138185590966614 1104 48-49 0.18085786708370932 1104 50-51 0.09537291457930053 1104 52-53 0.15222349379771316 1104 54-55 0.1296641029436003 1104 56-57 -0.31052672869871856 1104 58-59 -0.35815056675614443 1104 60-61 0.23168257141206539 1104 62-63 0.2327368036482369 1104 64-65 0.4380907929839566 1104 66-67 0.23863712710908658 1104 68-69 1.1788796688335452 1104 70-71 1.263279545151768 1104 72-73 1.1859685536614037 1104 74-75 0.32899837576498214 1104 76 -0.46418043848087365 1105 1 1.0126351109248262 1105 2 0.333412021041255 1105 3 0.42250663836695423 1105 4 0.07859493354944647 1105 5 -0.07212389547262887 1105 6 0.18013227682028088 1105 7 0.8555314167541042 1105 8 -0.6377372359438667 1105 9 0.4303520366237734 1105 10-11 0.6068719235991011 1105 12-13 -0.15601463099785917 1105 14-15 0.306943026540317 1105 16-17 0.5771504939825789 1105 18-19 0.753940457366987 1105 20-21 0.46106007616835853 1105 22-23 0.14807893213919954 1105 24-25 0.7716707012075545 1105 26-27 0.19754646278720855 1105 28-29 0.715169505383578 1105 30-31 1.307249708412897 1105 32-33 0.8103013033096573 1105 34-35 -0.37292788317606096 1105 36-37 0.3042536207296962 1105 38-39 0.1453743514756951 1105 40-41 0.5550755785175099 1105 42-43 0.34892331520232034 1105 44-45 0.8731551558178126 1105 46-47 0.9228005950248033 1105 48-49 0.06708541199388662 1105 50-51 0.6612411780523573 1105 52-53 0.5174929548755642 1105 54-55 0.599723983183118 1105 56-57 0.3451618940557708 1105 58-59 0.3394542236630187 1105 60-61 0.956233469615654 1105 62-63 -0.39001768736972764 1105 64-65 0.5398872001695807 1105 66-67 0.25660119896537026 1105 68-69 0.28965811194731117 1105 70-71 0.9069921200020659 1105 72-73 0.8446511883919428 1105 74-75 -0.27279803142064196 1105 76 0.5298315375670342 1106 1 -0.11760441003325894 1106 2 0.41406321864604223 1106 3 0.34410094974420247 1106 4 0.10063834672310179 1106 5 -0.044391859544489876 1106 6 0.42814874388614754 1106 7 -0.4346257688746391 1106 8 0.18733761435553475 1106 9 -0.04487251427443084 1106 10-11 0.2837244685092912 1106 12-13 1.198214410918311 1106 14-15 -0.015081674058485817 1106 16-17 0.3892013921861661 1106 18-19 0.6524995891035132 1106 20-21 0.351928339641411 1106 22-23 0.7321171057918932 1106 24-25 -0.07189217304394191 1106 26-27 0.8072957142842156 1106 28-29 0.648721152090161 1106 30-31 0.6930655766763749 1106 32-33 0.3037893272617538 1106 34-35 0.7555526557460936 1106 36-37 1.2053202374961671 1106 38-39 0.4724888724337788 1106 40-41 0.783761955763012 1106 42-43 0.17714187807657567 1106 44-45 0.736834048033387 1106 46-47 0.7800423614918657 1106 48-49 1.0616961904369973 1106 50-51 0.10291408224397713 1106 52-53 0.7065086734384352 1106 54-55 0.2720106598298244 1106 56-57 0.14761324136116372 1106 58-59 0.32682323563906834 1106 60-61 0.620492451651586 1106 62-63 0.771602821612305 1106 64-65 -0.4220701351597569 1106 66-67 0.04793727680968374 1106 68-69 1.5092127526658814 1106 70-71 0.9289232577266162 1106 72-73 0.884209571625469 1106 74-75 0.3950911901362417 1106 76 0.4169198609203235 1107 1 0.5095384471095983 1107 2 -0.6529010670682425 1107 3 -0.05964905025579981 1107 4 0.1061740610088151 1107 5 0.12257242616979624 1107 6 0.41404160102900534 1107 7 0.6934099454110765 1107 8 0.22340904292696706 1107 9 -0.7130867999887158 1107 10-11 0.015956611366434004 1107 12-13 -0.21839273193883457 1107 14-15 0.07893618308437311 1107 16-17 0.5102728207575993 1107 18-19 0.34116030338922343 1107 20-21 0.20978548249855322 1107 22-23 -0.1270793227795366 1107 24-25 0.2845363983846312 1107 26-27 -0.371007857144356 1107 28-29 0.553989009233014 1107 30-31 0.1514584338192293 1107 32-33 0.916289327261758 1107 34-35 -0.3867687728253344 1107 36-37 0.3998738089247382 1107 38-39 0.17132815814807145 1107 40-41 0.07367267004872957 1107 42-43 0.7496418780765808 1107 44-45 0.34049476231910347 1107 46-47 0.6144173614918671 1107 48-49 0.5445533332941395 1107 50-51 0.5744319393868338 1107 52-53 0.2257051020098615 1107 54-55 -0.24397148302731608 1107 56-57 -0.1537260443531281 1107 58-59 0.1253053784962077 1107 60-61 0.8017424516515845 1107 62-63 0.27579925018373785 1107 64-65 0.21319772198309295 1107 66-67 -0.765366294618886 1107 68-69 0.579837752665874 1107 70-71 -1.048576742273383 1107 72-73 -0.030968999803100417 1107 74-75 -1.2588373812923237 1107 76 -0.2950444247939643 1108 1 -0.7203651462295753 1108 2 0.11747579533315644 1108 3 0.003418434406782467 1108 4 0.22329938966788632 1108 5 -0.09688419083283151 1108 6 -0.36261045856784335 1108 7 -0.0022254621261765806 1108 8 -0.26872066785305293 1108 9 -0.33313938544007726 1108 10-11 0.22009333353996396 1108 12-13 0.8836055152127926 1108 14-15 0.43394056520531876 1108 16-17 -0.5633292826604546 1108 18-19 0.1856475952384855 1108 20-21 0.726314842708895 1108 22-23 -0.058507894208105427 1108 24-25 0.5285679496554465 1108 26-27 -0.38068358019430804 1108 28-29 -1.3685526209159704 1108 30-31 -0.3821261411150374 1108 32-33 0.3132218425991766 1108 34-35 0.4489192201632761 1108 36-37 1.0918808203182522 1108 38-39 0.7434052834767257 1108 40-41 0.3042949312231329 1108 42-43 -1.1391924777516422 1108 44-45 0.1437550603033273 1108 46-47 0.06840509155321683 1108 48-49 0.3629231843020335 1108 50-51 0.3458780392991869 1108 52-53 -0.38568841245113816 1108 54-55 -0.8522799843419548 1108 56-57 -0.19377862980448413 1108 58-59 -0.17288918767381745 1108 60-61 0.6741350896883915 1108 62-63 0.6498045087288702 1108 64-65 -0.14210464436221315 1108 66-67 -0.0015450851535092625 1108 68-69 0.672230390702687 1108 70-71 -0.8729939201874899 1108 72-73 -0.42763858788372744 1108 74-75 -1.1729302822563952 1108 76 0.18428949282216678 1109 1 -1.7647472671761157 1109 2 0.20424179007461163 1109 3 0.32892237831563165 1109 4 0.17474548958023917 1109 5 0.07685814045551354 1109 6 -0.3459583989709998 1109 7 -0.31801862601749775 1109 8 0.11483761435553674 1109 9 0.41834177143985585 1109 10-11 0.31595661136643116 1109 12-13 0.11589298234687817 1109 14-15 0.41322189737008586 1109 16-17 0.5617013921861655 1109 18-19 0.09830316053208321 1109 20-21 0.938356911069981 1109 22-23 0.11006353436332006 1109 24-25 -0.2411778873296555 1109 26-27 -0.0567221428586393 1109 28-29 -0.9545824193384149 1109 30-31 0.43431557667636866 1109 32-33 0.8420036129760433 1109 34-35 0.004659798603242393 1109 36-37 0.7598738089247377 1109 38-39 0.7970424438623525 1109 40-41 0.4222440986201548 1109 42-43 0.5639275923622904 1109 44-45 0.26049476231909807 1109 46-47 0.39441736149186823 1109 48-49 0.6045533332941417 1109 50-51 -0.07128234632745745 1109 52-53 -0.23429489799013226 1109 54-55 -0.34111434017017217 1109 56-57 -0.08515461578169692 1109 58-59 -0.048980335789508445 1109 60-61 0.6074567373658653 1109 62-63 0.15865639304087864 1109 64-65 0.6617691505545267 1109 66-67 0.21749084823825626 1109 68-69 -0.603019390191271 1109 70-71 0.22570897201233464 1109 72-73 -0.2338261426602415 1109 74-75 -0.17598023843518718 1109 76 0.44209843234889945 1110 1 -0.7264279394450242 1110 2 -0.5730691342951317 1110 3 -0.2761196384910889 1110 4 -0.1975234179827865 1110 5 0.348622846337868 1110 6 0.13219286153320553 1110 7 0.5061830546547696 1110 8 0.6218964378849492 1110 9 0.9396863092549808 1110 10-11 -0.803959355020126 1110 12-13 0.29858205797713566 1110 14-15 0.437675678882691 1110 16-17 0.3840543333626343 1110 18-19 -0.19841952854354616 1110 20-21 0.6357518690531734 1110 22-23 -0.6320373059728155 1110 24-25 -0.732186290690997 1110 26-27 0.9427736554606909 1110 28-29 -0.07609502438042881 1110 30-31 -0.4550961880295077 1110 32-33 0.7574657978499886 1110 34-35 0.6412144204519734 1110 36-37 1.0437393551432237 1110 38-39 0.8079668136102498 1110 40-41 -0.9829659854134576 1110 42-43 -0.11918165133518954 1110 44-45 0.5491502245039754 1110 46-47 0.7173585379624612 1110 48-49 0.8881667786722929 1110 50-51 -0.4274168001089649 1110 52-53 0.7706630852031466 1110 54-55 -0.30758492840546836 1110 56-57 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2116 40-41 -0.5062734215954734 2116 42-43 -0.513502776011471 2116 44-45 0.05061156375666087 2116 46-47 -0.6780354686968124 2116 48-49 0.1547779514432861 2116 50-51 -0.8847234963723807 2116 52-53 0.33235379922459884 2116 54-55 -0.5125608810506748 2116 56-57 0.2244410715498404 2116 58-59 -2.0128976763106152 2116 60-61 -0.9837921395433824 2116 62-63 0.5878567524299143 2116 64-65 -1.339542619436486 2116 66-67 -0.7380886665865418 2116 68-69 -1.2309798573970099 2116 70-71 0.9461402388586961 2116 72-73 0.21718913137390672 2116 74-75 0.28730817126832164 2116 76 -1.3610282522872197 2117 1 0.8871125710988181 2117 2 0.41897674065862134 2117 3 0.2047534654674763 2117 4 0.05452985615706041 2117 5 0.17270719705928883 2117 6 0.38809371243960555 2117 7 0.6943443569115217 2117 8 0.559114343915283 2117 9 -0.6759259733939302 2117 10-11 -0.1641332358932317 2117 12-13 -0.8609443941131332 2117 14-15 0.2560071624195075 2117 16-17 -0.9561602430339562 2117 18-19 -0.694257486368187 2117 20-21 0.28793462894958566 2117 22-23 0.5672782693139027 2117 24-25 -1.170162613295517 2117 26-27 -0.7553025561560318 2117 28-29 -0.10743057746958584 2117 30-31 0.5258879037204025 2117 32-33 -0.9950314274552241 2117 34-35 0.6548844167523811 2117 36-37 -0.5840075926925152 2117 38-39 0.7205824258928999 2117 40-41 0.8585064526183572 2117 42-43 0.4676292994602207 2117 44-45 -0.4965582475640957 2117 46-47 -0.6245763491999554 2117 48-49 0.01955782565712383 2117 50-51 0.2756538621181832 2117 52-53 0.8763789564573088 2117 54-55 0.25159006234554937 2117 56-57 0.5514850967070757 2117 58-59 -0.11667126121627547 2117 60-61 0.30551603655724335 2117 62-63 0.09729071469406847 2117 64-65 -0.38985708484528914 2117 66-67 -0.06198803765572336 2117 68-69 0.6495232872570753 2117 70-71 0.11595155961341419 2117 72-73 0.9058683766569189 2117 74-75 1.1269308127777578 2117 76 1.3873994206687499 2118 1 -0.2970522627939971 2118 2 9.113781552443356E-4 2118 3 -0.6775631606852457 2118 4 0.06379018721389684 2118 5 0.21599924474999455 2118 6 0.14659199541988954 2118 7 0.6726211636406987 2118 8 0.6821995775457239 2118 9 -1.6337528823725904 2118 10-11 -1.037575378116486 2118 12-13 -1.2912411105540826 2118 14-15 0.44927798851820455 2118 16-17 -3.296458117016286 2118 18-19 -0.47386160721550397 2118 20-21 -0.22153791802730183 2118 22-23 0.6868908787980246 2118 24-25 0.47335322572906335 2118 26-27 -2.3438737642433907 2118 28-29 -0.12315384790984041 2118 30-31 -0.7655617239371253 2118 32-33 0.05862061560531373 2118 34-35 0.2526002017583693 2118 36-37 -2.9878737809087426 2118 38-39 0.08082859636016337 2118 40-41 -1.1251529215375982 2118 42-43 0.40068482286185514 2118 44-45 -2.2979627311077166 2118 46-47 0.246319201982665 2118 48-49 0.4886900554676785 2118 50-51 -0.8749808564063315 2118 52-53 -1.2997988418989834 2118 54-55 -0.3062677144032975 2118 56-57 0.5178778119133085 2118 58-59 -0.9581652612934448 2118 60-61 -0.7491777937471937 2118 62-63 -1.1385390495533372 2118 64-65 0.3272205090120224 2118 66-67 0.33894571239250126 2118 68-69 -1.4412665418126451 2118 70-71 -0.9374110981015988 2118 72-73 0.45272951027578046 2118 74-75 -0.022623533790138595 2118 76 -0.26356326791403006 2119 1 0.7282860009256424 2119 2 -0.832940206011493 2119 3 0.014049579881188379 2119 4 -0.4859198724549856 2119 5 -0.028895284202022253 2119 6 -0.7578957766617975 2119 7 -0.6326565907924504 2119 8 0.11593350476649533 2119 9 -0.022441007425115345 2119 10-11 -0.16785943560030603 2119 12-13 0.15583427393200822 2119 14-15 -0.21409708501738578 2119 16-17 0.10307125519987181 2119 18-19 -0.08641308212936138 2119 20-21 -1.038140153509282 2119 22-23 -0.1543983051670068 2119 24-25 0.909272210517706 2119 26-27 0.2706946868869551 2119 28-29 -0.058085354759157326 2119 30-31 0.9523977684571889 2119 32-33 0.3950564505494327 2119 34-35 0.5597478612255458 2119 36-37 -0.08607531044903993 2119 38-39 -1.1284957166073184 2119 40-41 -0.9499281127301415 2119 42-43 0.5033405082135616 2119 44-45 0.8667569932388659 2119 46-47 0.10414338888912766 2119 48-49 -0.18008463148080978 2119 50-51 0.8324945616960306 2119 52-53 0.7485035364521337 2119 54-55 0.7380637420216019 2119 56-57 -0.9002035394607546 2119 58-59 -0.24659286025134008 2119 60-61 0.5750301228844563 2119 62-63 -0.382635192086326 2119 64-65 -0.8555889707761963 2119 66-67 0.3685280302343372 2119 68-69 0.8128514512960194 2119 70-71 -0.4695356463829725 2119 72-73 0.635665051077531 2119 74-75 0.1524542038348784 2119 76 0.275053422564163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 3.0 23 7.0 24 25.0 25 52.0 26 86.0 27 156.0 28 280.0 29 481.0 30 817.0 31 1372.0 32 2078.0 33 3355.0 34 4869.0 35 7949.0 36 13752.0 37 26061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.64993071970006 24.60184204091613 12.387317629798678 28.360909609585132 2 17.067405656532724 19.54193495802429 39.942945635341104 23.447713750101883 3 16.99568016953297 25.86355856223001 30.913684896894612 26.227076371342406 4 13.422446817181513 20.31298394327166 37.89061863232537 28.373950607221452 5 12.938299779933166 37.054364658896404 37.30866411280463 12.6986714483658 6 27.138316081180207 40.22332708452196 17.277691743418373 15.360665090879452 7 26.430841959409896 29.043931860787346 23.923710163827533 20.60151601597522 8 22.767951748308747 37.5319911973266 21.08892330263265 18.611133751732005 9 22.733719129513407 16.80169532969272 22.658733393104573 37.8058521476893 10-11 24.390740891678213 26.81473632732904 27.529546010269783 21.26497677072296 12-13 23.78433450158937 23.20401010677317 26.864455130817504 26.147200260819954 14-15 21.173689787268728 25.22617980275491 23.530035047681146 30.07009536229522 16-17 22.749205314206534 28.5760860705844 25.28567935447062 23.389029260738447 18-19 22.22185997228788 27.246719374032114 26.83348276143125 23.697937892248756 20-21 20.858260656940256 26.86853044257886 26.35015078653517 25.923058113945718 22-23 21.846931290243703 28.491319585948325 26.333849539489773 23.327899584318203 24-25 22.34248920042383 28.805118591572253 26.209960061944738 22.642432146059175 26-27 21.805363110277938 28.347053549596545 27.662401173689787 22.185182166435734 28-29 21.162278914336945 26.293911484228545 29.393593609911157 23.15021599152335 30-31 21.547803406960632 25.809764446980193 30.546091776020866 22.09634037003831 32-33 21.973265954845544 27.376314288043034 26.932105306055913 23.718314451055505 34-35 21.43287961529057 26.97774879778303 28.855652457412994 22.733719129513407 36-37 21.530687097562964 28.7578449751406 27.249164561088925 22.462303366207514 38-39 21.11908060966664 27.15380226587334 28.002282174586355 23.724834949873667 40-41 20.94873257804222 26.071399462058846 29.145814654821095 23.834053305077838 42-43 22.038470943027143 25.386747086152088 29.656858749694354 22.917923221126415 44-45 20.118184041079143 26.67617572744315 29.762816855489444 23.442823375988265 46-47 22.17621648056076 24.58309560681392 28.907001385606 24.333686527019317 48-49 20.61374195125927 24.80316244192681 29.70331730377374 24.879778303040183 50-51 19.14499959246882 25.398973021436138 30.82239791344038 24.633629472654658 52-53 18.998288369060234 25.42423995435651 32.55440541201402 23.023066264569238 54-55 18.037329855733965 25.976037166843263 30.879452278099272 25.1071806993235 56-57 19.70413236612601 25.496780503708532 29.55416089330834 25.24492623685712 58-59 17.985980927540957 26.219740810171977 31.212812780177686 24.581465482109383 60-61 19.620180943842204 27.60534680903089 28.065857038063413 24.708615209063495 62-63 17.828673893552857 29.624256255603555 27.49449832912218 25.05257152172141 64-65 18.40166272719863 30.130409976363193 26.071399462058846 25.396527834379327 66-67 18.180780829733475 29.764446980193988 25.806504197571112 26.24826799250143 68-69 17.855570951177764 29.940500448284297 25.65734778710571 26.54658081343223 70-71 18.365799983698754 28.874398891515202 25.756785394082648 27.0030157307034 72-73 19.0480071725487 27.685222919553347 25.55383486836743 27.712935039530525 74-75 18.86054283152661 27.758578531257644 26.452033580568912 26.928845056646832 76 18.792077593935936 27.49205314206537 26.758497025022415 26.95737223897628 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 3.5 17 7.0 18 8.0 19 11.0 20 15.5 21 18.0 22 23.5 23 40.0 24 78.5 25 106.0 26 150.5 27 206.0 28 291.0 29 365.0 30 418.0 31 570.5 32 813.0 33 956.0 34 1075.0 35 1399.0 36 1746.0 37 1888.0 38 2191.0 39 2973.0 40 3878.0 41 4419.5 42 4535.0 43 4592.5 44 4638.0 45 4724.0 46 4822.0 47 4692.0 48 4306.0 49 3911.5 50 3773.0 51 3394.0 52 2697.0 53 2135.0 54 1891.0 55 1751.5 56 1366.5 57 973.5 58 826.0 59 652.5 60 405.5 61 268.0 62 204.0 63 174.5 64 128.5 65 85.0 66 52.0 67 46.0 68 36.5 69 18.0 70 10.0 71 11.0 72 8.0 73 3.5 74 3.0 75 4.0 76 2.5 77 2.0 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 61345.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.04654006031461 #Duplication Level Percentage of deduplicated Percentage of total 1 56.695790309769656 23.271660282011574 2 16.485305798252583 13.533295297090229 3 9.444003177124701 11.629309642187627 4 5.734710087370929 9.415600293422447 5 3.443208895949166 7.066590594180456 6 2.1803018268467036 5.369630776754422 7 1.5607625099285147 4.484473062189257 8 1.068308181096108 3.5080283641698586 9 0.7029388403494837 2.5967886543320566 >10 2.652899126290707 15.569321053060559 >50 0.019857029388403495 0.5754340207025838 >100 0.003971405877680699 0.20865596218110682 >500 0.003971405877680699 0.8460347216562067 >1k 0.003971405877680699 1.9251772760616188 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 1181 1.9251772760616188 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 519 0.8460347216562067 RNA PCR Primer, Index 48 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 128 0.20865596218110682 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 86 0.14019072459043116 No Hit CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT 80 0.1304099763631918 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 78 0.12714972695411197 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008150623522699487 0.0 2 0.0 0.0 0.0 0.009780748227239384 0.0 3 0.0 0.0 0.0 0.011410872931779281 0.0 4 0.0 0.0 0.0 0.014671122340859075 0.0 5 0.0 0.0 0.0 0.021191621159018664 0.0 6 0.0 0.0 0.0 0.021191621159018664 0.0 7 0.0 0.0 0.0 0.021191621159018664 0.0 8 0.0 0.0 0.0 0.021191621159018664 0.0 9 0.0 0.0 0.0 0.04727361643165702 0.0 10 0.0 0.0 0.0 0.07661586111337518 0.0 11 0.0 0.0 0.0 0.08476648463607465 0.0 12 0.0 0.0 0.0 0.09454723286331404 0.0 13 0.0 0.0 0.0 0.09454723286331404 0.0 14 0.0 0.0 0.0 0.10432798109055343 0.0 15 0.0 0.0 0.0 0.11410872931779281 0.0 16 0.0 0.0 0.0 0.11573885402233271 0.0 17 0.0 0.0 0.0 0.127149726954112 0.0 18 0.0 0.0 0.0 0.13367022577227158 0.0 19 0.0 0.0 0.0 0.15486184693129024 0.0 20 0.0 0.0 0.0 0.16627271986306952 0.0 21 0.0 0.0 0.0 0.17116309397668922 0.0 22 0.0 0.0 0.0 0.1793137174993887 0.0 23 0.0 0.0 0.0 0.19724508924932757 0.0 24 0.0 0.0 0.0 0.21028608688564676 0.0 25 0.0 0.0 0.0 0.21191621159018664 0.0 26 0.0 0.0 0.0 0.21517646099926643 0.0 27 0.0 0.0 0.0 0.24940907979460428 0.0 28 0.0 0.0 0.0 0.2869019479990219 0.0 29 0.0 0.0 0.0 0.3292851903170593 0.0 30 0.0 0.0 0.0 0.3684081832260168 0.0 31 0.0 0.0 0.0 0.4075311761349743 0.0 32 0.0 0.0 0.0 0.4645855407938707 0.0 33 0.0 0.0 0.0 0.5330507783845464 0.0 34 0.0 0.0 0.0 0.6096666394979215 0.0 35 0.0 0.0 0.0 0.7400766158611134 0.0 36 0.0 0.0 0.0 0.9405819545195208 0.0 37 0.0 0.0 0.0 1.3269215094954765 0.0 38 0.0 0.0 0.0 1.7197815632895916 0.0 39 0.0 0.0 0.0 2.288695085174016 0.0 40 0.0 0.0 0.0 2.9782378351943923 0.0 41 0.0 0.0 0.0 3.8666557991686363 0.0 42 0.0 0.0 0.0 4.960469475914907 0.0 43 0.0 0.0 0.0 6.139049637297253 0.0 44 0.0 0.0 0.0 7.099193088271253 0.0 45 0.0 0.0 0.0 8.043035292199853 0.0 46 0.0 0.0 0.0 9.114027223082566 0.0 47 0.0 0.0 0.0 10.118184041079143 0.0 48 0.0 0.0 0.0 10.95443801450811 0.0 49 0.0 0.0 0.0 11.860787350232293 0.0 50 0.0 0.0 0.0 12.623685711956965 0.0 51 0.0 0.0 0.0 13.575678539408266 0.0 52 0.0 0.0 0.0 14.570054609177602 0.0 53 0.0 0.0 0.0 15.672018909446573 0.0 54 0.0 0.0 0.0 16.610970739261553 0.0 55 0.0 0.0 0.0 17.543402070258374 0.0 56 0.0 0.0 0.0 18.428559784823538 0.0 57 0.0 0.0 0.0 19.101801287798516 0.0 58 0.0 0.0 0.0 19.86306952481865 0.0 59 0.0 0.0 0.0 20.66346075474774 0.0 60 0.0 0.0 0.0 21.397016871790694 0.0 61 0.0 0.0 0.0 22.151764609992664 0.0 62 0.0 0.0 0.0 22.88532072703562 0.0 63 0.0 0.0 0.0 23.574863477055995 0.0 64 0.0 0.0 0.0 24.44698019398484 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTCG 20 7.868989E-5 70.00001 23 CGATTAG 20 7.868989E-5 70.00001 52 CGTTCGG 20 7.868989E-5 70.00001 24 TTGCGAT 20 7.868989E-5 70.00001 49 GCGATTA 20 7.868989E-5 70.00001 51 TTCGGTA 20 7.868989E-5 70.00001 26 GTAAGCA 20 7.868989E-5 70.00001 30 ATGCCAT 20 7.868989E-5 70.00001 43 TCGGTAA 20 7.868989E-5 70.00001 27 CCATTGC 20 7.868989E-5 70.00001 46 GGGTACA 25 2.809018E-6 70.0 62 GGTAAGC 25 2.3731633E-4 55.999996 29 GTTCGGT 25 2.3731633E-4 55.999996 25 TGCGATT 25 2.3731633E-4 55.999996 50 ATTGCGA 25 2.3731633E-4 55.999996 48 CGGTAAG 25 2.3731633E-4 55.999996 28 GGTACAA 20 0.0069208397 52.500004 63 CCAGGGA 20 0.0069208397 52.500004 4 GTTAGTG 20 0.0069208397 52.500004 30 TCAATCC 20 0.0069208397 52.500004 37 >>END_MODULE