FastQCFastQC Report
Wed 25 May 2016
SRR1780689_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780689_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484814
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGC207.822756E-444.033334
GGTACAC207.8706874E-443.9787227
ACGGTGC301.2879184E-436.724815
CGAGAAT502.3305129E-630.84563814
GACTTAG300.0057146829.36160712
AGATCTT300.005720460329.35553440
GACTGCC300.005720460329.35553435
AGACTTA300.005737830429.3373311
CGAGCTA300.00575524229.31914930
CGTGCGA554.9477367E-628.00381510
CATCGTA804.2491592E-927.53219824
GCTCGGA804.296453E-927.50374611
ATCGTAC804.300091E-927.50090225
CTCGCTA508.2593935E-526.43364713
GCTATAC508.435404E-526.3518523
GACATCG858.134521E-925.9046222
ACATCGT858.134521E-925.9046223
TCGTACT858.250936E-925.86983926
CATGTAC858.370989E-925.8351483
CGGTGGT609.797359E-625.64630943
Produced by FastQC (version 0.11.5)