FastQCFastQC Report
Wed 25 May 2016
SRR1780689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484814
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT161243.325811548346376No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT55071.1358995408548433Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC21870.4511008345468572No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG10830.22338463823239427No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC6130.1264402430622878No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT5480.11303303947493265No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4890.10086342391102568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT254.442092E-543.99484319
CCGTCTT5950.039.9322144
TTGGTAA404.1217572E-738.49548733
GCCGTCT6650.035.7288243
TTGTTCG250.0023527635.1958779
AGGTACT451.0331241E-634.2323344
TAGGTAC451.0331241E-634.2323343
TCTATAT353.2094755E-431.4378572
CGTTCGG701.033186E-931.42812724
CGGTAAG701.033186E-931.42488728
TGCCGTC7850.030.2670942
ATGCCGT23600.029.83009144
ACGCCCA300.00573916329.33594911
CGTTACA300.00573916329.33594914
TGCCGAG300.00573916329.33594915
GGTCAGA300.00574205729.33292224
TCGTTCG752.1609594E-929.3329223
TTCGGTA752.1609594E-929.3329226
AGTTATA300.005744952729.32989735
CACCGTA300.005744952729.32989730
Produced by FastQC (version 0.11.5)