FastQCFastQC Report
Wed 25 May 2016
SRR1780688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73628
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCT150.002217641669.99184447
GGTACCC150.002217641669.99184446
ATTAGAG150.002217641669.9918443
CGCGGTA150.002217641669.99184443
TTTAACG150.002217641669.99184434
GACAATG150.002217641669.99184418
GGCCGCG150.002217641669.99184440
TAACGGC150.002217641669.99184436
GCCGCGG150.002217641669.99184441
ACGGCCG150.002217641669.99184438
AGGCACC150.002217641669.9918448
GGGCGCA252.3776796E-455.9934811
CATATTC200.00692953252.493893
CGGCCGC200.00692953252.4938939
GACACAT200.00692953252.4938926
TTGCTAA200.00692953252.4938927
TGGAATT200.00692953252.4938913
CCCAGAA200.00692953252.493897
AACGGCC200.00692953252.4938937
ATTCATA200.00692953252.493892
Produced by FastQC (version 0.11.5)