##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780688_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73628 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.913402509914707 34.0 31.0 34.0 12.0 34.0 2 31.012685391427173 34.0 32.0 34.0 23.0 34.0 3 31.240533492692997 34.0 33.0 34.0 23.0 34.0 4 31.699299179659914 34.0 34.0 34.0 23.0 34.0 5 31.577185309936436 34.0 34.0 34.0 23.0 34.0 6 34.07839408920519 38.0 34.0 38.0 23.0 38.0 7 33.40506329113924 37.0 34.0 38.0 21.0 38.0 8 32.26051230510132 37.0 31.0 38.0 11.0 38.0 9 33.173629597435756 37.0 34.0 38.0 21.0 38.0 10-11 33.65704623241158 37.0 34.0 38.0 21.5 38.0 12-13 34.090271364154944 37.5 34.5 38.0 23.0 38.0 14-15 33.67223067311349 37.0 34.0 38.0 21.0 38.0 16-17 33.54539713152605 37.0 34.0 38.0 21.0 38.0 18-19 33.53260308578258 37.0 34.0 38.0 21.5 38.0 20-21 33.60589721301679 37.0 34.0 38.0 21.0 38.0 22-23 33.604124789482256 37.0 34.0 38.0 21.5 38.0 24-25 34.05081626555115 37.5 34.5 38.0 22.5 38.0 26-27 34.12030070082034 38.0 34.0 38.0 23.0 38.0 28-29 34.2712690824143 38.0 35.0 38.0 23.0 38.0 30-31 34.10712636496985 38.0 34.0 38.0 23.0 38.0 32-33 33.8450996903352 37.5 34.0 38.0 22.0 38.0 34-35 33.73514152224698 37.0 34.0 38.0 21.5 38.0 36-37 33.72362416471994 37.0 34.0 38.0 21.5 38.0 38-39 34.25195577769327 38.0 34.5 38.0 24.0 38.0 40-41 33.695224642798934 37.5 34.0 38.0 21.0 38.0 42-43 34.02719074265225 37.0 34.0 38.0 23.0 38.0 44-45 33.07555549519205 37.0 33.5 38.0 20.5 38.0 46-47 33.7931153908839 37.0 34.0 38.0 22.0 38.0 48-49 33.53595099690335 37.0 34.0 38.0 21.0 38.0 50-51 33.45251806378008 37.0 34.0 38.0 21.5 38.0 52-53 33.498635030151576 37.0 34.0 38.0 21.0 38.0 54-55 33.20903053186288 37.0 33.5 38.0 21.0 38.0 56-57 33.2794724832944 37.0 33.5 38.0 21.0 38.0 58-59 33.14514858477753 37.0 33.0 38.0 21.5 38.0 60-61 33.23120959417613 37.0 33.0 38.0 21.0 38.0 62-63 33.27414163090128 37.0 33.0 38.0 21.0 38.0 64-65 33.31143043407399 37.0 33.0 38.0 21.0 38.0 66-67 33.24698484272288 37.0 33.5 38.0 20.5 38.0 68-69 32.786494268484816 37.0 31.5 38.0 15.5 38.0 70-71 32.73161704786223 37.0 31.5 38.0 19.0 38.0 72-73 32.811797142391484 37.0 32.0 38.0 19.0 38.0 74-75 32.4300469929918 37.0 31.0 38.0 15.0 38.0 76 31.590020101048513 36.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.363489674108063 1101 2 -0.9146738771474006 1101 3 -0.4385066585845472 1101 4 -2.6350972645549113 1101 5 -1.5116487888846173 1101 6 -3.88572249341415 1101 7 -1.1475974818843326 1101 8 -1.1746890384103068 1101 9 -2.582436951789301 1101 10-11 -2.0729394225978623 1101 12-13 -1.580806473682344 1101 14-15 -1.0253469875102184 1101 16-17 -2.289948221811315 1101 18-19 -1.0668088489658984 1101 20-21 -1.3469230413086422 1101 22-23 -0.9635111774986385 1101 24-25 -1.8580898241849013 1101 26-27 -2.0254406742210733 1101 28-29 -1.0445462780751456 1101 30-31 -1.6143065763503728 1101 32-33 -0.8472369495044489 1101 34-35 -1.5876376773695924 1101 36-37 -0.21026859540739196 1101 38-39 -1.1793744262113606 1101 40-41 -0.5247274344148991 1101 42-43 -1.6120997170467035 1101 44-45 -0.8096978133527344 1101 46-47 -0.34356351863608126 1101 48-49 -1.2171770535427129 1101 50-51 -0.9081280127050491 1101 52-53 -0.664867704221173 1101 54-55 -1.1750997845591513 1101 56-57 -1.0219042321912326 1101 58-59 -0.5048103156677044 1101 60-61 -1.3953603174307077 1101 62-63 -2.2345738308610876 1101 64-65 -1.0626896374344597 1101 66-67 -0.9872216541988337 1101 68-69 -0.37716575838252453 1101 70-71 0.2850598615948101 1101 72-73 -0.7184564777741898 1101 74-75 -2.079323762412752 1101 76 -3.1180433280929236 1102 1 0.6955645616606496 1102 2 -0.4870069116610729 1102 3 0.29283578874848715 1102 4 0.1609583159022847 1102 5 0.3160069708643718 1102 6 0.9076437100820414 1102 7 1.040266704579139 1102 8 2.6454588773225467 1102 9 1.5115791844724669 1102 10-11 -0.06711243918235255 1102 12-13 -3.6966457834089255 1102 14-15 -1.2158445222480054 1102 16-17 -2.481426258566131 1102 18-19 -0.5651840170519549 1102 20-21 0.5981128170508399 1102 22-23 -0.6774006889284863 1102 24-25 0.6323012560481729 1102 26-27 -5.505270347013543 1102 28-29 0.2799998895626743 1102 30-31 0.3956665298800175 1102 32-33 -6.688675766178591 1102 34-35 -0.0931004743216306 1102 36-37 0.9487408174119736 1102 38-39 0.7561085410678814 1102 40-41 0.8134897327747055 1102 42-43 0.44233215207027854 1102 44-45 -5.542160834419526 1102 46-47 -0.22646356345903484 1102 48-49 0.3867281459194132 1102 50-51 -6.215193230096354 1102 52-53 -0.9813457409759891 1102 54-55 1.3528305157546043 1102 56-57 -0.8515209959742904 1102 58-59 1.151453691503967 1102 60-61 0.7403691760699687 1102 62-63 -4.262952360668347 1102 64-65 0.3740539752952614 1102 66-67 0.5573491660611438 1102 68-69 -7.336040702353841 1102 70-71 -0.10495582643746104 1102 72-73 0.9389561174745751 1102 74-75 -6.674992744931803 1102 76 -2.112048258617353 1103 1 0.020551125397396675 1103 2 -0.392342329651008 1103 3 0.5746553030886474 1103 4 0.5316037451298214 1103 5 0.8477165521415486 1103 6 -0.9328067711804806 1103 7 1.3694795838482037 1103 8 2.32907152206775 1103 9 1.6282059495088674 1103 10-11 1.1457912594544766 1103 12-13 -0.6965724947002769 1103 14-15 0.025446336207068043 1103 16-17 0.014335211116772939 1103 18-19 0.05262758210766805 1103 20-21 -0.582421701930933 1103 22-23 -0.46604826795814347 1103 24-25 0.7372506868392037 1103 26-27 -0.041719264783615984 1103 28-29 0.5786635921082421 1103 30-31 0.3486029641400421 1103 32-33 -5.356458049854751 1103 34-35 -0.7575969808931831 1103 36-37 0.15342396589489482 1103 38-39 0.991743954171902 1103 40-41 0.670808831812181 1103 42-43 0.39909181396504323 1103 44-45 -4.43938563531853 1103 46-47 -0.09196656829608685 1103 48-49 0.35616675441113443 1103 50-51 0.3902570068704705 1103 52-53 0.35090861970217446 1103 54-55 0.7883120225704516 1103 56-57 1.2667059058677026 1103 58-59 1.0139152149319273 1103 60-61 1.410777638472858 1103 62-63 0.13644667192069448 1103 64-65 0.7146388191903128 1103 66-67 0.09949017353659428 1103 68-69 -2.833842041094254 1103 70-71 -0.02982268528340626 1103 72-73 1.0813194340329417 1103 74-75 -0.9708470469790029 1103 76 0.6731425363208388 1104 1 -1.6211867824916695 1104 2 0.36005568390939047 1104 3 -0.08826222338587542 1104 4 -0.022241013254724606 1104 5 -0.6603902076489199 1104 6 -0.7551836958272986 1104 7 1.2716088838323287 1104 8 1.5299604311133024 1104 9 -0.5700071618995537 1104 10-11 -0.43740790605948376 1104 12-13 -1.8938785560622122 1104 14-15 -0.4626259556816379 1104 16-17 -1.5117991683476149 1104 18-19 0.7276315504516262 1104 20-21 0.8652415103868023 1104 22-23 0.6190897170301852 1104 24-25 0.5586071801837278 1104 26-27 0.8347631951361123 1104 28-29 -0.9253841187242031 1104 30-31 -1.4559957795956393 1104 32-33 -0.5869394666669265 1104 34-35 0.23461372683261317 1104 36-37 -0.32333287666389054 1104 38-39 -0.4152153873097646 1104 40-41 0.44434111145571364 1104 42-43 0.9723474433443897 1104 44-45 -3.0812029100866667 1104 46-47 -0.0682358714548883 1104 48-49 0.5275311844435606 1104 50-51 1.1448546167936016 1104 52-53 -0.20071386606848307 1104 54-55 -0.6019122455535353 1104 56-57 0.3914252872595725 1104 58-59 0.7429793660464625 1104 60-61 -0.17847608610139076 1104 62-63 0.4232558966196649 1104 64-65 0.09793549258426282 1104 66-67 0.12697378994512576 1104 68-69 -3.556851633148497 1104 70-71 -0.46808659149443343 1104 72-73 0.7927641379944816 1104 74-75 -0.8994168558182025 1104 76 -0.5538920903146831 1105 1 -0.5845041668616879 1105 2 0.6722060983578046 1105 3 0.48803447225848373 1105 4 0.6384279446714629 1105 5 1.102149945550984 1105 6 0.8600419483086483 1105 7 1.6204419139107245 1105 8 1.4465091653047395 1105 9 1.5316169367593844 1105 10-11 1.0443294077756775 1105 12-13 -1.6039106786221282 1105 14-15 0.6710196179194554 1105 16-17 1.7183825599808884 1105 18-19 1.7072011530753386 1105 20-21 0.30461298645212054 1105 22-23 0.9128538970063644 1105 24-25 1.0252154421959858 1105 26-27 1.2813566313514926 1105 28-29 -0.2225290296571103 1105 30-31 0.5418477408572215 1105 32-33 1.0034978943412298 1105 34-35 1.3322056580049733 1105 36-37 1.4850773935699522 1105 38-39 1.365239270461423 1105 40-41 1.3155588484999896 1105 42-43 1.2638315954660513 1105 44-45 1.7091580756041935 1105 46-47 1.598363405225932 1105 48-49 0.5565650366797854 1105 50-51 0.22494761497407012 1105 52-53 -0.12660734496418513 1105 54-55 -0.1879467941529498 1105 56-57 0.6936244471310786 1105 58-59 0.6929086065613248 1105 60-61 0.9960078189236512 1105 62-63 1.481784099328749 1105 64-65 1.331497951869487 1105 66-67 1.18519873771789 1105 68-69 1.8967166666021598 1105 70-71 1.6912907250363247 1105 72-73 1.5240802644180889 1105 74-75 -1.2381189246907702 1105 76 -0.38108170327153346 1106 1 -0.35411200999894277 1106 2 0.0506927042677745 1106 3 -0.1455612195393634 1106 4 -0.04259939584220618 1106 5 0.3762454992654227 1106 6 0.17715048186291682 1106 7 0.9057912649187401 1106 8 1.0077491542580646 1106 9 1.4150055034536635 1106 10-11 0.935692290957121 1106 12-13 0.10083248624945185 1106 14-15 -0.34109720063895566 1106 16-17 0.6473386494589377 1106 18-19 -0.1519537765021326 1106 20-21 -0.3068548401151219 1106 22-23 0.37967935958576504 1106 24-25 0.49863438723879483 1106 26-27 0.5938048500755997 1106 28-29 -0.9934486651169223 1106 30-31 -0.592887139010216 1106 32-33 0.7582617717231699 1106 34-35 0.9240311205843739 1106 36-37 0.6779453858372833 1106 38-39 0.055759844888385146 1106 40-41 0.18227384435748206 1106 42-43 0.8541726614699172 1106 44-45 1.208950950500629 1106 46-47 0.7051363961124295 1106 48-49 0.29662303170877635 1106 50-51 0.5323557895114917 1106 52-53 0.24545838273535026 1106 54-55 0.19665000231211138 1106 56-57 1.466373687672366 1106 58-59 0.3144565215019455 1106 60-61 1.8652428368744935 1106 62-63 1.1109611222505933 1106 64-65 1.2116070377573607 1106 66-67 1.4063738274721018 1106 68-69 1.6471309168092816 1106 70-71 0.38395159220009845 1106 72-73 0.7868184582871933 1106 74-75 -1.030890258576438 1106 76 -0.5674252547766407 1107 1 -0.21167636275426105 1107 2 0.0815913196380933 1107 3 0.5414799476384076 1107 4 0.10980279726252107 1107 5 0.060986694321645274 1107 6 0.5837809065446393 1107 7 0.3931762663126577 1107 8 0.058929341972962845 1107 9 -1.3338793585480069 1107 10-11 -0.11487224765453163 1107 12-13 0.07356968539451714 1107 14-15 -0.04327404293985637 1107 16-17 0.9313968224911804 1107 18-19 1.0905422740507973 1107 20-21 0.44601431702641037 1107 22-23 -1.112967699237764 1107 24-25 -0.3551663747721676 1107 26-27 0.49382575891306857 1107 28-29 -0.9782084458064517 1107 30-31 0.4173233432869665 1107 32-33 1.1388026136523592 1107 34-35 0.631028611523881 1107 36-37 -0.25889830898661614 1107 38-39 0.7903221532240394 1107 40-41 0.5926097796794778 1107 42-43 0.15738565282759964 1107 44-45 -0.2308548296313333 1107 46-47 -0.12277225549987492 1107 48-49 -0.21729296122867936 1107 50-51 0.4281716988135926 1107 52-53 0.5144157287069149 1107 54-55 0.003951832996996529 1107 56-57 0.18613718548855474 1107 58-59 0.2864270632731589 1107 60-61 -0.4541512714797449 1107 62-63 -1.0223211005911494 1107 64-65 -0.24744648886656506 1107 66-67 1.1587318796977257 1107 68-69 0.82966506791049 1107 70-71 -0.18385112130236791 1107 72-73 0.5031203819002386 1107 74-75 -0.22203188110222527 1107 76 -1.502212913916125 1108 1 -0.5416907112041933 1108 2 -0.4078430035933529 1108 3 -0.6275274005071623 1108 4 -0.6512520312082799 1108 5 -0.46797108645143126 1108 6 0.4826460745954222 1108 7 1.129077827250093 1108 8 1.1205841965318513 1108 9 -0.062193633081683686 1108 10-11 0.0488196519932842 1108 12-13 -0.06203403650643935 1108 14-15 0.5473494624299491 1108 16-17 -0.6170074559557079 1108 18-19 -0.09996600891803098 1108 20-21 0.10695609710379728 1108 22-23 -0.817096139604736 1108 24-25 -0.5922092896815201 1108 26-27 -0.8222097208903989 1108 28-29 -0.2231212681030712 1108 30-31 -0.002020976031275268 1108 32-33 -0.30784724068913505 1108 34-35 -0.6752673143859411 1108 36-37 -0.539247454601643 1108 38-39 0.07633458854731856 1108 40-41 0.06374982924685213 1108 42-43 0.03975010345588714 1108 44-45 -0.020856568837380962 1108 46-47 -0.26448493866001144 1108 48-49 -0.08352627572061522 1108 50-51 -0.5202391016559815 1108 52-53 -0.6696792319435474 1108 54-55 0.2433535461149603 1108 56-57 0.132613119224807 1108 58-59 -1.746683071950109 1108 60-61 0.5084340583171993 1108 62-63 -0.598878777011862 1108 64-65 -0.6403222660742642 1108 66-67 -0.3734651723480056 1108 68-69 0.5486419799501405 1108 70-71 1.1265536789063404 1108 72-73 0.19406346638217542 1108 74-75 0.7229548574516258 1108 76 -0.3861426499916085 1109 1 0.4904193805389241 1109 2 -0.15554942561170293 1109 3 0.11100470958458075 1109 4 -0.04116028808901362 1109 5 0.6008362529981071 1109 6 0.9734717626688152 1109 7 -0.6599129984379815 1109 8 0.9049878662566258 1109 9 1.4056962751752025 1109 10-11 1.594691211626312 1109 12-13 0.3072320185394304 1109 14-15 1.08404671013637 1109 16-17 0.025194378906675752 1109 18-19 0.41838261493518303 1109 20-21 1.4601671062781136 1109 22-23 1.4260231264503105 1109 24-25 1.3183411690340847 1109 26-27 0.9759554167242825 1109 28-29 0.8456860713464636 1109 30-31 1.202107464335704 1109 32-33 1.6806848694026115 1109 34-35 0.8935400250635936 1109 36-37 0.928642453655236 1109 38-39 0.8951419279968533 1109 40-41 1.1738415723661149 1109 42-43 1.13608037868525 1109 44-45 0.8002443485938144 1109 46-47 0.08413891455099787 1109 48-49 0.4623452839124127 1109 50-51 1.440770072655944 1109 52-53 1.36472443778122 1109 54-55 0.8236287754727556 1109 56-57 0.6738975228945279 1109 58-59 0.27395912988475146 1109 60-61 0.7997184619869273 1109 62-63 0.37589186518997053 1109 64-65 0.20600800915509154 1109 66-67 0.7985531762758455 1109 68-69 1.8215777597666474 1109 70-71 0.5164619357870706 1109 72-73 0.08397172326290558 1109 74-75 1.8789181602039235 1109 76 0.5909215701918811 1110 1 -0.4843399632492087 1110 2 -0.06226972208207826 1110 3 -1.1159804217029858 1110 4 -0.4635401859513557 1110 5 -0.03788567044383839 1110 6 -1.0338270125831386 1110 7 -0.08283522015805289 1110 8 1.141599246018906 1110 9 -1.4442381940253242 1110 10-11 -1.4529993926128526 1110 12-13 -1.0904043533139571 1110 14-15 -0.802279949600603 1110 16-17 -0.7657058786971547 1110 18-19 -0.8350408044167423 1110 20-21 -1.0344096551445787 1110 22-23 -0.9996672066269312 1110 24-25 -1.0945028676631665 1110 26-27 -2.629210218020603 1110 28-29 -0.6399762416647832 1110 30-31 -0.9550647686827816 1110 32-33 0.35939187893846736 1110 34-35 -0.7675075638549202 1110 36-37 -1.0661263808004051 1110 38-39 -0.29750732404815494 1110 40-41 -0.40469370128145954 1110 42-43 -0.6736091409062084 1110 44-45 0.580026967114172 1110 46-47 -0.28241552120622515 1110 48-49 -0.33466741550459034 1110 50-51 -0.6696267276333003 1110 52-53 -1.2902559029789344 1110 54-55 -0.9467987565017566 1110 56-57 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48-49 0.7397926072312941 2116 50-51 0.04030127539814998 2116 52-53 0.5449089321859049 2116 54-55 0.3771876019673641 2116 56-57 -0.043941977055062864 2116 58-59 -0.6760358292845225 2116 60-61 -1.4957246270341429 2116 62-63 0.6703771963725487 2116 64-65 1.3057408451264578 2116 66-67 0.07910061049300054 2116 68-69 0.13824274615640775 2116 70-71 -2.7971785521653416 2116 72-73 0.861721496425524 2116 74-75 -1.2759613639416614 2116 76 -1.6182224967503558 2117 1 0.28322070086318263 2117 2 0.27307054429267197 2117 3 0.7225526926921972 2117 4 0.10150268943634799 2117 5 -0.5762910709182059 2117 6 -1.2801324313023486 2117 7 -3.0644079258686894 2117 8 -3.285512594886036 2117 9 0.7779840281968973 2117 10-11 -0.06730335174439972 2117 12-13 1.3795828752939627 2117 14-15 0.08323364280953882 2117 16-17 -0.16599779623044952 2117 18-19 0.5517499272015769 2117 20-21 0.23068795478061332 2117 22-23 0.8738047875347235 2117 24-25 0.947825174664878 2117 26-27 1.4611017688431076 2117 28-29 0.7675223899717736 2117 30-31 0.19372194906945595 2117 32-33 1.6944584726257546 2117 34-35 0.4227861781629585 2117 36-37 -1.0479727591985082 2117 38-39 0.4594713294822199 2117 40-41 -1.4410921687144125 2117 42-43 -0.4346928847485714 2117 44-45 0.8593555567875839 2117 46-47 -0.29007835645526825 2117 48-49 -0.175754808316114 2117 50-51 0.8866586217554939 2117 52-53 1.3235007107776795 2117 54-55 -0.5933048685251094 2117 56-57 -0.8749350580481448 2117 58-59 -0.6071510103996971 2117 60-61 0.745728364418845 2117 62-63 -0.3880273620320054 2117 64-65 -0.28430799492237924 2117 66-67 -1.7169921855997927 2117 68-69 0.024893132806795393 2117 70-71 1.4592317042449139 2117 72-73 1.7115383462423708 2117 74-75 -0.14958773756803367 2117 76 0.1548747763469187 2118 1 -0.47459425695177515 2118 2 0.7172003884225155 2118 3 0.9684557385952459 2118 4 0.8137864017200584 2118 5 0.26649457186743675 2118 6 0.7623386111686941 2118 7 1.1483190868583222 2118 8 -5.0124681218415645 2118 9 -1.0558212056083391 2118 10-11 -0.17340945785051076 2118 12-13 1.2300190257301082 2118 14-15 -1.0642424046665084 2118 16-17 -0.31543294566559865 2118 18-19 -0.12853035307870186 2118 20-21 -1.875389551296891 2118 22-23 0.5951404088703427 2118 24-25 0.051443078282780164 2118 26-27 1.1847825925239306 2118 28-29 0.10300072545011574 2118 30-31 0.5913338466813514 2118 32-33 0.9045256826929631 2118 34-35 1.0982759536527382 2118 36-37 0.42181131058556076 2118 38-39 0.0906167606276469 2118 40-41 -0.5498008774231238 2118 42-43 -0.01963496969065659 2118 44-45 0.40754660497863426 2118 46-47 -0.28668925306616444 2118 48-49 -0.8297945623558718 2118 50-51 0.34291487801175435 2118 52-53 -0.677514590237621 2118 54-55 0.3005318253115803 2118 56-57 1.8794764963634094 2118 58-59 1.2267114234627385 2118 60-61 -0.5757216570311741 2118 62-63 1.3027205287158807 2118 64-65 0.387163476549091 2118 66-67 -1.9941693627769723 2118 68-69 1.823363031276692 2118 70-71 -0.43935259433938256 2118 72-73 -1.7997872650832392 2118 74-75 1.0025358145555145 2118 76 0.3658285873007294 2119 1 0.33826006078059123 2119 2 1.1223091557264055 2119 3 0.17880511235924956 2119 4 0.5913078255829447 2119 5 0.26804368195313444 2119 6 1.4933867324607135 2119 7 -1.6469347035174167 2119 8 1.6593512585143984 2119 9 -0.3292556156281137 2119 10-11 0.7303479581020298 2119 12-13 0.6658133566463889 2119 14-15 -0.5197796492281412 2119 16-17 0.281963230998862 2119 18-19 -0.24443345129887462 2119 20-21 -1.4150566574274066 2119 22-23 1.198007910518335 2119 24-25 0.1585459128246356 2119 26-27 1.0152539175074509 2119 28-29 1.2699420570255882 2119 30-31 0.42307412354357865 2119 32-33 1.0306792753099643 2119 34-35 1.5137011349315728 2119 36-37 0.5232945010931402 2119 38-39 0.726312212176758 2119 40-41 -0.14807048849760918 2119 42-43 -0.042706822030794456 2119 44-45 -5.779830240015826E-4 2119 46-47 0.6080536605791877 2119 48-49 -0.06361130592871689 2119 50-51 1.3701726235621763 2119 52-53 -0.5289318611670879 2119 54-55 -0.8052031779843958 2119 56-57 0.8920671357833143 2119 58-59 1.8632967893163936 2119 60-61 1.2438894174579502 2119 62-63 0.40573634941463155 2119 64-65 0.7211120592781626 2119 66-67 0.8433059173812367 2119 68-69 -0.584217720206496 2119 70-71 -0.17331765696298618 2119 72-73 -1.542091154338351 2119 74-75 -0.05725324279451627 2119 76 0.4775293124160882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 14.0 13 107.0 14 261.0 15 512.0 16 701.0 17 724.0 18 730.0 19 762.0 20 676.0 21 605.0 22 640.0 23 648.0 24 712.0 25 839.0 26 958.0 27 1148.0 28 1349.0 29 1799.0 30 2194.0 31 2728.0 32 3482.0 33 4789.0 34 6421.0 35 9571.0 36 15075.0 37 16180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.918507985123604 25.940713191861736 11.298129512141763 27.842649310872897 2 16.79252458303906 20.431085999891348 36.29597435758136 26.480415059488237 3 17.938827619927203 25.604389634378226 29.12343130330852 27.33335144238605 4 12.673167816591512 18.641006138968873 39.725376215570165 28.96044982886945 5 13.873798011626013 36.33400336828381 35.2922801108274 14.49991850926278 6 26.561905796707773 37.60390068995491 17.58298473406856 18.251208779268758 7 25.191503232465912 29.837833432933124 24.627858966697453 20.342804367903515 8 21.567881784103875 34.07806812625632 19.954365187157062 24.39968490248275 9 23.34573803444342 15.39360026077036 22.668006736567612 38.59265496821862 10-11 23.99358939533873 25.43529635464769 27.35644048459825 23.21467376541533 12-13 23.18071929157386 23.591568425055684 26.41318520128212 26.814527082088336 14-15 21.524420057586788 25.344977454229365 24.14910088553268 28.981501602651168 16-17 22.251724887271145 27.48343021676536 25.568397892106265 24.696447003857227 18-19 21.621529852773403 26.513011354376054 26.824034334763947 25.0414244580866 20-21 20.90101591785734 27.3829249741946 26.599934807410225 25.11612430053784 22-23 22.636089531156625 26.997881240832296 26.359537132612594 24.006492095398492 24-25 21.925082848916173 27.731976965284943 26.3113217797577 24.03161840604118 26-27 20.611723800727987 28.47286358450589 27.22741348400065 23.68799913076547 28-29 22.014043570380835 26.97411310914326 27.94589015048623 23.06595316998968 30-31 21.797414027272232 26.92318139838105 27.628076275330038 23.651328299016676 32-33 20.789645243657304 28.616830553593744 26.515727712283372 24.077796490465584 34-35 21.86192752757103 27.45083392187755 27.66610528603249 23.021133264518934 36-37 22.177025044819906 27.1778399521921 26.801624382028578 23.843510620959417 38-39 21.554979084044113 27.45151301135438 27.796490465583744 23.197017439017763 40-41 21.641902537078288 26.27193459010159 28.049111750964308 24.037051121855814 42-43 21.02596838159396 26.558510349323626 28.32889661541805 24.08662465366437 44-45 20.42904873146086 26.154452110610094 28.469468137121744 24.9470310208073 46-47 21.077579181833 25.882137230401476 29.013418808062152 24.026864779703374 48-49 19.949611560819253 26.701798228934642 29.27079371978052 24.077796490465584 50-51 20.10648122996686 26.190443852882055 28.751290270005974 24.951784647145107 52-53 19.35880371597762 26.52930950181996 29.011381539631664 25.100505242570758 54-55 18.878008366382353 27.14116912044331 28.634486879991307 25.346335633183024 56-57 19.412451784647146 26.684820992013908 28.800863801814526 25.101863421524417 58-59 19.452518063780083 26.81113163470419 28.119057967077744 25.617292334437984 60-61 19.60870864345086 26.297060900744285 28.16116151464117 25.93306894116369 62-63 19.771011028413106 26.307247242896725 28.03552996142772 25.886211767262456 64-65 20.073205845602217 26.56598033356875 27.464415711414137 25.896398109414896 66-67 20.317678057260824 26.69500733416635 26.620307491715106 26.367007116857717 68-69 20.086108545661975 26.79958711359809 26.462079643613844 26.652224697126094 70-71 20.734638996034118 26.737110881729777 26.079752268158853 26.448497854077253 72-73 20.874531428260987 26.93268865105666 25.38096919650133 26.81181072418102 74-75 20.36453523116206 27.27970337371652 25.70082034008801 26.65494105503341 76 21.745803227033196 26.523876786005324 25.58673330798066 26.143586678980824 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.5 15 2.0 16 1.5 17 1.0 18 3.0 19 8.0 20 11.5 21 12.0 22 24.0 23 41.0 24 53.5 25 61.0 26 113.5 27 206.0 28 289.5 29 333.0 30 421.0 31 584.0 32 817.5 33 976.0 34 1183.0 35 1619.0 36 2021.5 37 2195.0 38 2511.0 39 3171.5 40 3930.5 41 4395.0 42 4445.0 43 4722.0 44 5294.0 45 5593.5 46 5598.0 47 5631.5 48 5370.5 49 4800.0 50 4524.0 51 4298.0 52 3688.5 53 2992.5 54 2680.0 55 2540.0 56 2036.5 57 1470.5 58 1268.0 59 1071.0 60 753.5 61 536.0 62 439.0 63 376.5 64 275.5 65 212.0 66 151.0 67 115.0 68 104.0 69 78.0 70 61.5 71 60.0 72 47.0 73 26.0 74 19.0 75 20.0 76 17.0 77 10.0 78 5.5 79 5.0 80 6.0 81 5.0 82 4.0 83 5.0 84 5.5 85 4.0 86 1.0 87 0.0 88 1.0 89 1.0 90 1.0 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6682240452001955 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 73628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.744716683870266 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14360938247965 42.640028250122235 2 10.292005744375299 11.680339001466834 3 5.205840114887506 8.862117672624544 4 2.8267113451412156 6.4160373770848045 5 1.8477740545715653 5.242570761123486 6 1.1943513642891335 4.066387787254849 7 0.8688367640019148 3.451132721247351 8 0.5600765916706558 2.5425110012495247 9 0.4739109621828626 2.4202748954202207 >10 1.5868836764001915 12.67860053240615 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.005432715814635737 0.0 3 0.0 0.0 0.0 0.0067908947682946705 0.0 4 0.0 0.0 0.0 0.021730863258542947 0.0 5 0.0 0.0 0.0 0.023089042212201878 0.0 6 0.0 0.0 0.0 0.023089042212201878 0.0 7 0.0 0.0 0.0 0.02987993698049655 0.0 8 0.0 0.0 0.0 0.033954473841473355 0.0 9 0.0 0.0 0.0 0.05704351605367523 0.0 10 0.0 0.0 0.0 0.09235616884880751 0.0 11 0.0 0.0 0.0 0.10322160047807899 0.0 12 0.0 0.0 0.0 0.11272885315369154 0.0 13 0.0 0.0 0.0 0.1195197479219862 0.0 14 0.0 0.0 0.0 0.123594284782963 0.0 15 0.0 0.0 0.0 0.13310153745857553 0.0 16 0.0 0.0 0.0 0.13717607431955234 0.0 17 0.0 0.0 0.0 0.14532514804150595 0.0 18 0.0 0.0 0.0 0.15619057967077743 0.0 19 0.0 0.0 0.0 0.17248872711468463 0.0 20 0.0 0.0 0.0 0.18607051665127397 0.0 21 0.0 0.0 0.0 0.20372684304884012 0.0 22 0.0 0.0 0.0 0.20915955886347584 0.0 23 0.0 0.0 0.0 0.2145922746781116 0.0 24 0.0 0.0 0.0 0.22274134840006518 0.0 25 0.0 0.0 0.0 0.22817406421470093 0.0 26 0.0 0.0 0.0 0.23360678002933666 0.0 27 0.0 0.0 0.0 0.26077035910251534 0.0 28 0.0 0.0 0.0 0.2906502960830119 0.0 29 0.0 0.0 0.0 0.3191720541098495 0.0 30 0.0 0.0 0.0 0.34769381213668715 0.0 31 0.0 0.0 0.0 0.391155538653773 0.0 32 0.0 0.0 0.0 0.43597544412451783 0.0 33 0.0 0.0 0.0 0.47672081273428585 0.0 34 0.0 0.0 0.0 0.5351225077416201 0.0 35 0.0 0.0 0.0 0.660074971478242 0.0 36 0.0 0.0 0.0 0.8502200249904928 0.0 37 0.0 0.0 0.0 1.1598848264247297 0.0 38 0.0 0.0 0.0 1.565980333568751 0.0 39 0.0 0.0 0.0 2.0291193567664476 0.0 40 0.0 0.0 0.0 2.6280762753300375 0.0 41 0.0 0.0 0.0 3.3682838050741566 0.0 42 0.0 0.0 0.0 4.020209702830445 0.0 43 0.0 0.0 0.0 4.771282664203835 0.0 44 0.0 0.0 0.0 5.484326614874776 0.0 45 0.0 0.0 0.0 6.3209648503286795 0.0 46 0.0 0.0 0.0 7.07203781170207 0.0 47 0.0 0.0 0.0 7.798663551909599 0.0 48 0.0 0.0 0.0 8.617645460965937 0.0 49 0.0 0.0 0.0 9.420329222578367 0.0 50 0.0 0.0 0.0 10.133373173249307 0.0 51 0.0 0.0 0.0 10.786657249959255 0.0 52 0.0 0.0 0.0 11.612430053783887 0.0 53 0.0 0.0 0.0 12.32004128864019 0.0 54 0.0 0.0 0.0 13.062965176291629 0.0 55 0.0 0.0 0.0 13.704025642418646 0.0 56 0.0 0.0 0.0 14.361384255989568 0.0 57 0.0 0.0 0.0 15.009235616884881 0.0 58 0.0 0.0 0.0 15.68560873580703 0.0 59 0.0 0.0 0.0 16.21801488564133 0.0 60 0.0 0.0 0.0 16.829195414787854 0.0 61 0.0 0.0 0.0 17.422719617536806 0.0 62 0.0 0.0 0.0 18.067854620524802 0.0 63 0.0 0.0 0.0 18.73064594991036 0.0 64 0.0 0.0 0.0 19.30108111044711 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCT 15 0.0022176416 69.991844 47 GGTACCC 15 0.0022176416 69.991844 46 ATTAGAG 15 0.0022176416 69.991844 3 CGCGGTA 15 0.0022176416 69.991844 43 TTTAACG 15 0.0022176416 69.991844 34 GACAATG 15 0.0022176416 69.991844 18 GGCCGCG 15 0.0022176416 69.991844 40 TAACGGC 15 0.0022176416 69.991844 36 GCCGCGG 15 0.0022176416 69.991844 41 ACGGCCG 15 0.0022176416 69.991844 38 AGGCACC 15 0.0022176416 69.991844 8 GGGCGCA 25 2.3776796E-4 55.99348 11 CATATTC 20 0.006929532 52.49389 3 CGGCCGC 20 0.006929532 52.49389 39 GACACAT 20 0.006929532 52.49389 26 TTGCTAA 20 0.006929532 52.49389 27 TGGAATT 20 0.006929532 52.49389 13 CCCAGAA 20 0.006929532 52.49389 7 AACGGCC 20 0.006929532 52.49389 37 ATTCATA 20 0.006929532 52.49389 2 >>END_MODULE