FastQCFastQC Report
Wed 25 May 2016
SRR1780688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73628
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT10211.3867007116857717No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4210.5717933394904112TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1660.22545770630738307No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1620.22002499049274732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCT150.00221661770.000019
CCCACGC150.00221661770.000017
ATCGTAT150.00221661770.0000139
AGTCTGC150.00221661770.0000144
AAATTCG150.00221661770.0000151
TGAATTG150.00221661770.0000143
GTACGGG150.00221661770.000011
TGCAAAT150.00221661770.0000148
GCCGTAG308.302746E-658.3333361
GGTGTAC252.3763122E-455.99999610
TACGGGC252.3763122E-455.9999968
GGCCCTA252.3763122E-455.99999612
ACGGGCC252.3763122E-455.9999969
CCCGTGC252.3763122E-455.9999968
GTACTCG252.3763122E-455.99999613
CGGTGTA252.3763122E-455.9999969
TACTCGT252.3763122E-455.99999614
TCGGTGT252.3763122E-455.9999968
AATACGG252.3763122E-455.9999966
GTCGGTG252.3763122E-455.9999967
Produced by FastQC (version 0.11.5)