FastQCFastQC Report
Wed 25 May 2016
SRR1780687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554413
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT301.293907E-436.69702537
CGTTTCG250.00234216535.22914536
CTCGCTA451.0255517E-634.2660413
CGTATTC401.8305174E-532.9677229
TGACGGT300.005706554729.37089313
CTGACGT300.00571160129.36558212
CCATGGA300.005719177429.3576240
TATGACC300.005782623329.2914284
TGACCGG300.005782623329.2914286
TAACTGG407.019277E-427.50536322
CAGCGAA407.056426E-427.4805425
CGAATTT508.225657E-526.45054819
CGAAGAG756.8694135E-826.45054618
TATCGGA959.713403E-1025.44876926
CGACAGC701.1222892E-625.16367136
GGCCTTA701.1436932E-625.109244
ATCAGGT450.001390536524.46468210
CGTCAGC450.00139567124.44921122
AATGCCT450.001408945824.4095215
TTCGGTA1001.7607817E-924.1763326
Produced by FastQC (version 0.11.5)